Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-1258
(620 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC14F5.09c |ade8||adenylosuccinate lyase Ade8|Schizosaccharomy... 210 2e-55
SPBC947.02 |apl2||AP-1 adaptor complex subunit Apl2 |Schizosacch... 28 1.3
SPBC119.09c |||ORMDL family protein|Schizosaccharomyces pombe|ch... 27 1.7
SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyc... 27 2.9
SPBC27B12.03c |||lathosterol oxidase |Schizosaccharomyces pombe|... 27 2.9
SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyc... 26 3.8
SPCC1442.07c |||ubiquitin/metalloprotease fusion protein|Schizos... 26 5.1
SPAC1952.16 |rga9||RhoGAp, GTPase activator towards Rho/Rac/Cdc4... 26 5.1
SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|ch... 25 6.7
SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 25 6.7
SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual 25 6.7
SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr 3||... 25 8.8
SPBC1539.03c |||argininosuccinate lyase|Schizosaccharomyces pomb... 25 8.8
>SPBC14F5.09c |ade8||adenylosuccinate lyase Ade8|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 482
Score = 210 bits (512), Expect = 2e-55
Identities = 102/190 (53%), Positives = 139/190 (73%), Gaps = 3/190 (1%)
Frame = +3
Query: 60 EYCNYRSPLSTRYASKEMQYNFSDQKKFSTWRKLWIYLAKAEKELGL-DISAAQIAELEA 236
+Y +Y +PL+ RYAS EM + FS + + +TWR+LW+ LA AEK+LGL I+ I +L+A
Sbjct: 3 DYGSYSTPLTARYASAEMSHLFSREMRINTWRQLWLNLAIAEKQLGLTQITDEAIEQLKA 62
Query: 237 AVHDI--DFPAAAEHEKRVRHDVMAHVHTLGERCPLAAPIIHLGATSCYVGDNTDLIVLK 410
V +F AA+ EKR RHDVMAH++T G P A+ IIHLGATSC+V DN DLI L+
Sbjct: 63 HVKITAPEFEIAAKEEKRQRHDVMAHIYTYGLAAPAASGIIHLGATSCFVTDNADLIFLR 122
Query: 411 NGLELLLPRLVAVIKQLGSFAKQYKSLPILGFTHLQPAQLTTVGKRASLWLNDLLMDERA 590
+ ++LL+P+LV VI +L ++ +YK +P LGFTH QPAQLTTVGKRA+LW+ +LL D R
Sbjct: 123 SAMDLLIPKLVNVINRLSQWSLRYKDIPTLGFTHYQPAQLTTVGKRATLWIQELLWDLRN 182
Query: 591 LSRAKDDLGF 620
RA++D+GF
Sbjct: 183 FVRARNDIGF 192
>SPBC947.02 |apl2||AP-1 adaptor complex subunit Apl2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 683
Score = 27.9 bits (59), Expect = 1.3
Identities = 20/63 (31%), Positives = 32/63 (50%), Gaps = 1/63 (1%)
Frame = +3
Query: 417 LELLLPRLVAVIKQLGSFAKQYKSLPILG-FTHLQPAQLTTVGKRASLWLNDLLMDERAL 593
++LLLP L VI++LG + ILG F+H+ P + + S ++ L + AL
Sbjct: 427 IDLLLPYLSRVIEELGDPRARSSMAWILGEFSHVIPTSSKLLSEMISTMADEDLQIQLAL 486
Query: 594 SRA 602
A
Sbjct: 487 LTA 489
>SPBC119.09c |||ORMDL family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 186
Score = 27.5 bits (58), Expect = 1.7
Identities = 14/38 (36%), Positives = 23/38 (60%)
Frame = -1
Query: 227 FGYLSGAYIQSELLFSFSQINPEFTPSRKFLLVTEIVL 114
F + GAY + + + N ++TP+RK+LLV I+L
Sbjct: 94 FEFNGGAYDRLTMWEQLDEGN-QYTPARKYLLVLPIIL 130
>SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 781
Score = 26.6 bits (56), Expect = 2.9
Identities = 12/32 (37%), Positives = 17/32 (53%)
Frame = +3
Query: 252 DFPAAAEHEKRVRHDVMAHVHTLGERCPLAAP 347
D+ ++A HE+RV H + H G PL P
Sbjct: 61 DYKSSA-HEQRVNHTTDSMAHVPGNNSPLQTP 91
>SPBC27B12.03c |||lathosterol oxidase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 329
Score = 26.6 bits (56), Expect = 2.9
Identities = 20/58 (34%), Positives = 25/58 (43%)
Frame = -1
Query: 308 MRHHVVTNPLLMFSSRWEVDVVYSSF*FGYLSGAYIQSELLFSFSQINPEFTPSRKFL 135
+ H + PL +W V YSS F YL G Y QS F P F P K++
Sbjct: 173 LHHRWLYAPLHKLHHKWIVPTPYSSHAFHYLDG-YSQSLPYHMF----PFFFPLNKYV 225
>SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1428
Score = 26.2 bits (55), Expect = 3.8
Identities = 20/74 (27%), Positives = 31/74 (41%)
Frame = -2
Query: 610 SSLARDKARSSISKSLSHSDALLPTVVSCAG*RWVKPRMGKDLYCFAKLPSCLMTATSLG 431
+ + +KAR S +K + SD +L CA +G D FAK + A S+
Sbjct: 1154 NEFSANKARLSFAKENTRSDLVLMYYAYCAWREICLSPLGPDEDSFAKEKYLNLEALSMT 1213
Query: 430 RSSSSPFLRTIKSV 389
S L +K +
Sbjct: 1214 ESLKIQLLSELKDM 1227
>SPCC1442.07c |||ubiquitin/metalloprotease fusion
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 282
Score = 25.8 bits (54), Expect = 5.1
Identities = 10/29 (34%), Positives = 15/29 (51%)
Frame = +2
Query: 314 HARREVSPRRAHHTPGSHLVLRRRQHRFD 400
H R + +H G+H+ LR R R+D
Sbjct: 160 HTRHDSKTLGLNHNQGAHIELRLRTDRYD 188
>SPAC1952.16 |rga9||RhoGAp, GTPase activator towards
Rho/Rac/Cdc42-like small GTPases|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 684
Score = 25.8 bits (54), Expect = 5.1
Identities = 21/81 (25%), Positives = 35/81 (43%)
Frame = +3
Query: 30 IDAPVELQNTEYCNYRSPLSTRYASKEMQYNFSDQKKFSTWRKLWIYLAKAEKELGLDIS 209
+D V + NY S S + K N +D+K T RK + A LDI+
Sbjct: 119 VDEIVSAAIVKINNYNSRFSHVNSKKS---NLTDRKPIPTSRKSNKSDSLASALSQLDIN 175
Query: 210 AAQIAELEAAVHDIDFPAAAE 272
+ + + + ++ +D P AE
Sbjct: 176 PSNVNKFDGLINIVDHPYTAE 196
>SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 589
Score = 25.4 bits (53), Expect = 6.7
Identities = 16/47 (34%), Positives = 22/47 (46%), Gaps = 1/47 (2%)
Frame = -1
Query: 227 FGYLSGAYIQSEL-LFSFSQINPEFTPSRKFLLVTEIVLHFFGGISC 90
+ ++ AY +E + F I E RK +L T IVL FG C
Sbjct: 127 YSWIGTAYSLAETSILPFCGIMSEVV-GRKIVLYTSIVLFLFGSAMC 172
>SPCC1322.12c |bub1||serine/threonine protein kinase
Bub1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1044
Score = 25.4 bits (53), Expect = 6.7
Identities = 13/41 (31%), Positives = 25/41 (60%), Gaps = 3/41 (7%)
Frame = -1
Query: 500 EDGQRLVLFREASKLFDDGDEP---WQKQLESVLKDYQIGV 387
E+ QRL LF+E + ++ D+P W + +E +L+ +G+
Sbjct: 28 EEIQRLALFQEELDIIEELDDPVDVWYRCIEWLLETRFLGM 68
>SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 1279
Score = 25.4 bits (53), Expect = 6.7
Identities = 18/48 (37%), Positives = 26/48 (54%)
Frame = +3
Query: 348 IIHLGATSCYVGDNTDLIVLKNGLELLLPRLVAVIKQLGSFAKQYKSL 491
I+ LG S Y D+TD V G + R+V+ IKQ+ AK ++ L
Sbjct: 681 IVQLGKVS-YRPDSTDDDVYIRGFSKDVERVVSEIKQVVRDAKNHEIL 727
>SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1315
Score = 25.0 bits (52), Expect = 8.8
Identities = 13/43 (30%), Positives = 21/43 (48%), Gaps = 1/43 (2%)
Frame = -1
Query: 356 VYDGRGEGTPLAERVDMRHHVVTNPLLMFS-SRWEVDVVYSSF 231
++DG G GT + V HH P S S++ +D + S +
Sbjct: 36 IFDGPGLGTSIPSSVSSFHHQTLRPSSDASVSQFSMDYLQSEY 78
>SPBC1539.03c |||argininosuccinate lyase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 460
Score = 25.0 bits (52), Expect = 8.8
Identities = 14/47 (29%), Positives = 29/47 (61%), Gaps = 1/47 (2%)
Frame = +3
Query: 390 TDL-IVLKNGLELLLPRLVAVIKQLGSFAKQYKSLPILGFTHLQPAQ 527
TD+ + L++ L+++ LV +++ + A+ + + G+THLQ AQ
Sbjct: 119 TDMRLWLRDELDIIQKSLVGLLQVFVARAESDLDIIMPGYTHLQRAQ 165
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,524,304
Number of Sequences: 5004
Number of extensions: 50929
Number of successful extensions: 176
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 175
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 273658928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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