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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-1258
         (620 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g36280.1 68414.m04509 adenylosuccinate lyase, putative / aden...    56   2e-08
At4g18440.1 68417.m02736 adenylosuccinate lyase, putative / aden...    52   2e-07
At5g15020.1 68418.m01761 paired amphipathic helix repeat-contain...    30   1.1  
At3g15190.1 68416.m01920 chloroplast 30S ribosomal protein S20, ...    29   3.3  
At5g52280.1 68418.m06488 protein transport protein-related low s...    27   7.6  
At3g12480.1 68416.m01553 transcription factor, putative                27   7.6  
At1g26850.3 68414.m03275 dehydration-responsive family protein s...    27   7.6  
At1g26850.2 68414.m03274 dehydration-responsive family protein s...    27   7.6  
At1g26850.1 68414.m03273 dehydration-responsive family protein s...    27   7.6  
At1g20910.1 68414.m02618 ARID/BRIGHT DNA-binding domain-containi...    27   7.6  

>At1g36280.1 68414.m04509 adenylosuccinate lyase, putative /
           adenylosuccinase, putative similar to SP|P25739
           Adenylosuccinate lyase (EC 4.3.2.2) (Adenylosuccinase)
           {Escherichia coli}; contains Pfam profile PF00206: Lyase
          Length = 527

 Score = 56.0 bits (129), Expect = 2e-08
 Identities = 36/117 (30%), Positives = 62/117 (52%), Gaps = 7/117 (5%)
 Frame = +3

Query: 276 EKRVRHDVMAHVHTLGERC---PLAAPII---HLGATSCYVGDNTDLIVLKNGLE-LLLP 434
           E+   HDV A  + L ++C   P  A ++   H   TS  + + +  ++L+  L  ++LP
Sbjct: 150 ERVTNHDVKAVEYFLKQKCESHPEIAKVLEFFHFACTSEDINNLSHALMLQEALSSVILP 209

Query: 435 RLVAVIKQLGSFAKQYKSLPILGFTHLQPAQLTTVGKRASLWLNDLLMDERALSRAK 605
            +  +IK +   AK +  +P+L  TH QPA  TT+GK  +++   L ++ R LS  K
Sbjct: 210 TMDELIKSISLMAKSFAYVPMLSRTHGQPASPTTLGKEMAIFAVRLSVERRYLSETK 266


>At4g18440.1 68417.m02736 adenylosuccinate lyase, putative /
           adenylosuccinase, putative similar to SP|P25739
           Adenylosuccinate lyase (EC 4.3.2.2) (Adenylosuccinase)
           {Escherichia coli}; contains Pfam profile PF00206: Lyase
          Length = 536

 Score = 52.4 bits (120), Expect = 2e-07
 Identities = 36/117 (30%), Positives = 60/117 (51%), Gaps = 7/117 (5%)
 Frame = +3

Query: 276 EKRVRHDVMAHVHTLGERC---PLAAPII---HLGATSCYVGDNTDLIVLKNGLE-LLLP 434
           E+   HDV A  + L ++C   P  A ++   H   TS  + + +  ++L+  L  ++LP
Sbjct: 159 ERVTNHDVKAVEYFLKQKCESQPEIAKVLEFFHFACTSEDINNLSHALMLQEALSSVILP 218

Query: 435 RLVAVIKQLGSFAKQYKSLPILGFTHLQPAQLTTVGKRASLWLNDLLMDERALSRAK 605
            +  +IK +   AK +  +P+L  TH QPA  TT+GK  + +   L  + R LS  K
Sbjct: 219 TMDELIKSISLIAKNFAYVPMLSRTHGQPATPTTLGKEMANFAVRLSEERRYLSETK 275


>At5g15020.1 68418.m01761 paired amphipathic helix repeat-containing
           protein similar to transcription co-repressor Sin3
           [Xenopus laevis] GI:4960210; contains Pfam profile
           PF02671: Paired amphipathic helix repeat
          Length = 1377

 Score = 30.3 bits (65), Expect = 1.1
 Identities = 19/74 (25%), Positives = 32/74 (43%)
 Frame = -1

Query: 527 LRRLKMGEAEDGQRLVLFREASKLFDDGDEPWQKQLESVLKDYQIGVVSDVARGGSQVYD 348
           L  L M   ++     ++ E S LF+D  +  ++    +         + + R  +Q YD
Sbjct: 159 LEILNMYRKDNKDITEVYNEVSTLFEDHSDLLEEFTRFLPDSLAPHTEAQLLRSQAQRYD 218

Query: 347 GRGEGTPLAERVDM 306
            RG G PL  R+ M
Sbjct: 219 DRGSGPPLVRRMFM 232


>At3g15190.1 68416.m01920 chloroplast 30S ribosomal protein S20,
           putative contains Pfam profile: PF01649 ribosomal
           protein S20
          Length = 202

 Score = 28.7 bits (61), Expect = 3.3
 Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 1/55 (1%)
 Frame = -2

Query: 619 NPRSSLA-RDKARSSISKSLSHSDALLPTVVSCAG*RWVKPRMGKDLYCFAKLPS 458
           +P SS + R  A +++S SLS S ++   V    G  WV+  M + + C A  P+
Sbjct: 30  SPSSSFSNRRGASATLSSSLSFSQSVSQCVAFSTGNLWVQKPMRQLIVCEAAAPT 84


>At5g52280.1 68418.m06488 protein transport protein-related low
           similarity to  SP|P25386 Intracellular protein transport
           protein USO1 {Saccharomyces cerevisiae}
          Length = 853

 Score = 27.5 bits (58), Expect = 7.6
 Identities = 19/96 (19%), Positives = 43/96 (44%)
 Frame = +3

Query: 15  DKMTTIDAPVELQNTEYCNYRSPLSTRYASKEMQYNFSDQKKFSTWRKLWIYLAKAEKEL 194
           +K  T +  ++LQ T+  N    L+ R  ++ ++       + S+   L     K E+  
Sbjct: 361 EKDLTSNLKLQLQRTQESNSNLILAVRDLNEMLEQK---NNEISSLNSLLEEAKKLEEHK 417

Query: 195 GLDISAAQIAELEAAVHDIDFPAAAEHEKRVRHDVM 302
           G+D    +I  L+  + D+D+   +  +K    +++
Sbjct: 418 GMDSGNNEIDTLKQQIEDLDWELDSYKKKNEEQEIL 453


>At3g12480.1 68416.m01553 transcription factor, putative
          Length = 293

 Score = 27.5 bits (58), Expect = 7.6
 Identities = 9/26 (34%), Positives = 18/26 (69%)
 Frame = +3

Query: 471 AKQYKSLPILGFTHLQPAQLTTVGKR 548
           +++Y   P++  + + PAQL ++GKR
Sbjct: 256 SEEYSGWPMMDISKMDPAQLASLGKR 281


>At1g26850.3 68414.m03275 dehydration-responsive family protein
           similar to early-responsive to dehydration stress ERD3
           protein [Arabidopsis thaliana] GI:15320410; contains
           Pfam profile PF03141: Putative methyltransferase
          Length = 506

 Score = 27.5 bits (58), Expect = 7.6
 Identities = 14/43 (32%), Positives = 20/43 (46%), Gaps = 1/43 (2%)
 Frame = -1

Query: 452 DDGDEPWQKQLESVLKDYQIGVVSDVARGGS-QVYDGRGEGTP 327
           DD D+ W K++E+ +  Y     SD   GG  Q +  R    P
Sbjct: 380 DDTDDVWYKKMEACITPYPETSSSDEVAGGELQAFPDRLNAVP 422


>At1g26850.2 68414.m03274 dehydration-responsive family protein
           similar to early-responsive to dehydration stress ERD3
           protein [Arabidopsis thaliana] GI:15320410; contains
           Pfam profile PF03141: Putative methyltransferase
          Length = 616

 Score = 27.5 bits (58), Expect = 7.6
 Identities = 14/43 (32%), Positives = 20/43 (46%), Gaps = 1/43 (2%)
 Frame = -1

Query: 452 DDGDEPWQKQLESVLKDYQIGVVSDVARGGS-QVYDGRGEGTP 327
           DD D+ W K++E+ +  Y     SD   GG  Q +  R    P
Sbjct: 380 DDTDDVWYKKMEACITPYPETSSSDEVAGGELQAFPDRLNAVP 422


>At1g26850.1 68414.m03273 dehydration-responsive family protein
           similar to early-responsive to dehydration stress ERD3
           protein [Arabidopsis thaliana] GI:15320410; contains
           Pfam profile PF03141: Putative methyltransferase
          Length = 616

 Score = 27.5 bits (58), Expect = 7.6
 Identities = 14/43 (32%), Positives = 20/43 (46%), Gaps = 1/43 (2%)
 Frame = -1

Query: 452 DDGDEPWQKQLESVLKDYQIGVVSDVARGGS-QVYDGRGEGTP 327
           DD D+ W K++E+ +  Y     SD   GG  Q +  R    P
Sbjct: 380 DDTDDVWYKKMEACITPYPETSSSDEVAGGELQAFPDRLNAVP 422


>At1g20910.1 68414.m02618 ARID/BRIGHT DNA-binding domain-containing
           protein low similarity to Chain A, Human Mrf-2 Domain
           [Homo sapiens] GI:14278238; contains Pfam profile
           PF01388: ARID/BRIGHT DNA binding domain
          Length = 398

 Score = 27.5 bits (58), Expect = 7.6
 Identities = 10/15 (66%), Positives = 12/15 (80%)
 Frame = +1

Query: 100 PPKKCSTISVTKRNF 144
           PPK C+T+S T RNF
Sbjct: 170 PPKTCTTVSYTFRNF 184


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,367,684
Number of Sequences: 28952
Number of extensions: 278259
Number of successful extensions: 904
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 858
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 904
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1255974912
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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