Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-1218
(613 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g24140.1 68414.m03045 matrixin family protein similar to matr... 92 2e-19
At4g16640.1 68417.m02515 matrix metalloproteinase, putative meta... 82 3e-16
At2g45040.1 68415.m05607 matrix metalloproteinase nearly identic... 80 1e-15
At1g70170.1 68414.m08074 matrixin family protein similar to SP|P... 79 3e-15
At1g59970.1 68414.m06755 matrixin family protein similar to SP|P... 76 2e-14
At4g25940.1 68417.m03731 epsin N-terminal homology (ENTH) domain... 31 0.46
At1g67880.1 68414.m07751 glycosyl transferase family 17 protein ... 30 1.1
At2g44470.1 68415.m05529 glycosyl hydrolase family 1 protein con... 29 2.4
At3g43590.1 68416.m04638 zinc knuckle (CCHC-type) family protein... 29 3.2
At3g17170.1 68416.m02190 ribosomal protein S6 family protein (RF... 28 5.6
At4g29230.1 68417.m04181 no apical meristem (NAM) family protein... 27 9.8
At1g14110.1 68414.m01668 xyloglucan fucosyltransferase family pr... 27 9.8
At1g12990.1 68414.m01508 glycosyl transferase family 17 protein ... 27 9.8
>At1g24140.1 68414.m03045 matrixin family protein similar to matrix
metalloproteinase [Cucumis sativus] GI:7159629; contains
InterPro accession IPR001818: Matrixin
Length = 384
Score = 92.3 bits (219), Expect = 2e-19
Identities = 52/121 (42%), Positives = 66/121 (54%), Gaps = 6/121 (4%)
Frame = +2
Query: 254 ETRSVLARALDVWEQASRLTFTEVNS-EEADILVSFAKRYHDDAYLFDGRGTILAHAFFP 430
E +SV +RA WE+ + LTFT V +DI + F H D FDG LAHAF P
Sbjct: 188 EVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRTLAHAFSP 247
Query: 431 GVDRGGDAHFDDDELWLLEPKDDD-----EEGTSLFAVAVHEFGHSLGLSHSSVKGALMY 595
G H D +E W++ + D E L +VAVHE GH LGL HSSV+G++MY
Sbjct: 248 PT---GHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGHSSVEGSIMY 304
Query: 596 P 598
P
Sbjct: 305 P 305
>At4g16640.1 68417.m02515 matrix metalloproteinase, putative
metalloproteinase [Arabidopsis thaliana] GI:3128477;
contains InterPro accession IPR001818: Matrixin
Length = 364
Score = 81.8 bits (193), Expect = 3e-16
Identities = 48/131 (36%), Positives = 64/131 (48%), Gaps = 3/131 (2%)
Frame = +2
Query: 215 LSSTRRPSLLDPYETRSVLARALDVWEQASRLTFTEVNS-EEADILVSFAKRYHDDAYLF 391
+S T + L + ++V RA W ++F EV+ AD+ + F H D F
Sbjct: 168 ISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPF 227
Query: 392 DGRGTILAHAFFPGVDRGGDAHFDDDELWLLEP--KDDDEEGTSLFAVAVHEFGHSLGLS 565
DG LAHAF P G H D E W+++ K E L +VA HE GH LGL
Sbjct: 228 DGVLGTLAHAFAP---ENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLG 284
Query: 566 HSSVKGALMYP 598
HSS + A+MYP
Sbjct: 285 HSSQESAVMYP 295
>At2g45040.1 68415.m05607 matrix metalloproteinase nearly identical
to metalloproteinase [Arabidopsis thaliana] GI:3128477;
contains InterPro accession IPR001818: Matrixin
Length = 342
Score = 79.8 bits (188), Expect = 1e-15
Identities = 48/121 (39%), Positives = 58/121 (47%), Gaps = 2/121 (1%)
Frame = +2
Query: 242 LDPYETRSVLARALDVWEQASRLTFTEVNSEE-ADILVSFAKRYHDDAYLFDGRGTILAH 418
L P + R V RA W ++F E ADI + F H D FDG +LAH
Sbjct: 155 LAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAH 214
Query: 419 AFFPGVDRGGDAHFDDDELWLLE-PKDDDEEGTSLFAVAVHEFGHSLGLSHSSVKGALMY 595
F P G H D E W ++ ++ L +VAVHE GH LGL HSSVK A MY
Sbjct: 215 TFSP---ENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMY 271
Query: 596 P 598
P
Sbjct: 272 P 272
>At1g70170.1 68414.m08074 matrixin family protein similar to
SP|P29136 Metalloendoproteinase 1 precursor (EC
3.4.24.-) (SMEP1) {Glycine max}; contains InterPro
accession IPR001818: Matrixin
Length = 378
Score = 78.6 bits (185), Expect = 3e-15
Identities = 49/120 (40%), Positives = 60/120 (50%), Gaps = 5/120 (4%)
Frame = +2
Query: 254 ETRSVLARALDVWEQASRLTFTEVNS-EEADILVSFAKRYHDDAYLFDGRGTILAHAFFP 430
E +SV +RA W + L FT S +DI + F H D FDG LAHAF P
Sbjct: 184 EVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSP 243
Query: 431 GVDRGGDAHFDDDELWLLEPKDDD----EEGTSLFAVAVHEFGHSLGLSHSSVKGALMYP 598
G H D DE W++ D L +VAVHE GH LGL HSSV+ ++MYP
Sbjct: 244 P---SGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYP 300
>At1g59970.1 68414.m06755 matrixin family protein similar to
SP|P29136 Metalloendoproteinase 1 precursor (EC
3.4.24.-) (SMEP1) {Glycine max}; contains InterPro
accession IPR001818: Matrixin
Length = 360
Score = 76.2 bits (179), Expect = 2e-14
Identities = 49/126 (38%), Positives = 61/126 (48%), Gaps = 7/126 (5%)
Frame = +2
Query: 254 ETRSVLARALDVWEQASRLTFTEVNSE-EADILVSFAKRYHDDAYLFDGRGTILAHAFFP 430
E + V +RA W + + L FT S ADI++ F H D FDG LAHA P
Sbjct: 172 EVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSP 231
Query: 431 GVDRGGDAHFDDDELWLLEPKDDDEE------GTSLFAVAVHEFGHSLGLSHSSVKGALM 592
G H D DE WL+ + L +VAVHE GH LGL HSSV+ A+M
Sbjct: 232 PT---GMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIM 288
Query: 593 YPWYQG 610
+P G
Sbjct: 289 FPAISG 294
>At4g25940.1 68417.m03731 epsin N-terminal homology (ENTH)
domain-containing protein contains Pfam PF01417: ENTH
domain. ENTH (Epsin N-terminal homology) domain; similar
to Chain B, Crystal Structure Of N-Terminal Domain Of
Drosophila Ap180 (GP:13399617) [Drosophila
melanogaster]; supporting cDNA
gi|20465326|gb|AY096427.1|
Length = 601
Score = 31.5 bits (68), Expect = 0.46
Identities = 15/39 (38%), Positives = 24/39 (61%), Gaps = 1/39 (2%)
Frame = -3
Query: 554 TNVQ-TREQQQQKVMCLLHRHLWALTTRVRHHQSARHHR 441
TNVQ +QQQQ+ M ++H+ + T +HQ+ HH+
Sbjct: 526 TNVQMAMQQQQQQQMTMMHQSPYNYTHPHDYHQNHHHHQ 564
>At1g67880.1 68414.m07751 glycosyl transferase family 17 protein low
similarity to beta-1,4-mannosyl-glycoprotein
beta-1,4-N-acetylglucosaminyltransferase (EC 2.4.1.144)
from Mus musculus [SP|Q10470], Rattus norvegicus
[SP|Q02527], Homo sapiens [SP|Q09327] ; contains Pfam
profile PF04724 :Glycosyltransferase family 17
Length = 390
Score = 30.3 bits (65), Expect = 1.1
Identities = 14/39 (35%), Positives = 21/39 (53%)
Frame = +3
Query: 96 FCFMRVPLFNCTRKASNRFCYVLFADFLRPKENECQLCN 212
FCF R+ F KA + + V FA +L PK + +C+
Sbjct: 295 FCFRRISEFVFKMKAYSHYDRVRFAHYLNPKRVQRVICS 333
>At2g44470.1 68415.m05529 glycosyl hydrolase family 1 protein
contains Pfam PF00232 : Glycosyl hydrolase family 1
domain; TIGRFAM TIGR01233: 6-phospho-beta-galactosidase;
similar to anther-specific protein ATA27 (GI:2746341)
[Arabidopsis thaliana]
Length = 451
Score = 29.1 bits (62), Expect = 2.4
Identities = 15/37 (40%), Positives = 20/37 (54%), Gaps = 1/37 (2%)
Frame = +2
Query: 287 VWEQASRLTFTE-VNSEEADILVSFAKRYHDDAYLFD 394
+W+ S TF E N + AD+ V F RY DD L +
Sbjct: 62 IWDYFSH-TFPERTNMQNADVAVDFYHRYKDDIKLIE 97
>At3g43590.1 68416.m04638 zinc knuckle (CCHC-type) family protein
contains Pfam domain, PF00098: Zinc knuckle
Length = 551
Score = 28.7 bits (61), Expect = 3.2
Identities = 14/44 (31%), Positives = 18/44 (40%)
Frame = -3
Query: 461 QSARHHRGPRQERKRELESYLVHRIDMHRHGNVWRTRQGYRPLP 330
QS+RHH GP R + + H +H H Y P P
Sbjct: 493 QSSRHHSGPSPSRWQPSHQHHHHHQHLHHH----HQNHSYEPAP 532
>At3g17170.1 68416.m02190 ribosomal protein S6 family protein (RFC3)
annotation temporarily based on supporting cDNA
gi|15620809|dbj|AB057424.1|; contains TIGRfam TIGR00166
and Pfam PF01250 profiles ribosomal protein S6.
Length = 314
Score = 27.9 bits (59), Expect = 5.6
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +2
Query: 440 RGGDAHFDDDELWLLEPKDDDEEG 511
R GD ++DDDE +E +D+ EG
Sbjct: 229 RAGDEYYDDDEEEEIEEDEDEGEG 252
>At4g29230.1 68417.m04181 no apical meristem (NAM) family protein
similar to NAM family proteins GP|12751304|,
GP|6223650|, GP|9758909 - Arabidopsis
thaliana,PIR2:T04621
Length = 498
Score = 27.1 bits (57), Expect = 9.8
Identities = 12/48 (25%), Positives = 22/48 (45%)
Frame = -3
Query: 545 QTREQQQQKVMCLLHRHLWALTTRVRHHQSARHHRGPRQERKRELESY 402
QT + VM HRH ++ HH + HH Q++++ ++
Sbjct: 329 QTGHATCEDVMAEQHRHRHQPSSSTSHHMAHDHHHHHHQQQQQRHHAF 376
>At1g14110.1 68414.m01668 xyloglucan fucosyltransferase family
protein contains Pfam profile: PF03254 xyloglucan
fucosyltransferase
Length = 907
Score = 27.1 bits (57), Expect = 9.8
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = +3
Query: 99 CFMRVPLFNCTRKASNRFCYVLFAD 173
CF+R PL+ C K N +V++ +
Sbjct: 480 CFLRAPLYGCQAKKVNITPFVMYCE 504
>At1g12990.1 68414.m01508 glycosyl transferase family 17 protein low
similarity to beta-1,4-mannosyl-glycoprotein
beta-1,4-N-acetylglucosaminyltransferase (EC 2.4.1.144)
from Mus musculus [SP|Q10470], Rattus norvegicus
[SP|Q02527], Homo sapiens [SP|Q09327]; contains Pfam
profile PF04724 :Glycosyltransferase family 17
Length = 392
Score = 27.1 bits (57), Expect = 9.8
Identities = 14/38 (36%), Positives = 18/38 (47%)
Frame = +3
Query: 96 FCFMRVPLFNCTRKASNRFCYVLFADFLRPKENECQLC 209
FCF R+ F KA + V F FL PK + +C
Sbjct: 297 FCFRRISEFIFKMKAYSHNDRVRFGHFLNPKRVQRVIC 334
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,415,364
Number of Sequences: 28952
Number of extensions: 282225
Number of successful extensions: 896
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 861
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 890
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1226538000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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