Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-1196
(421 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 139 2e-34
SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 46 2e-06
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 35 0.006
SPAC1783.08c |rpl1502|rpl15-2|60S ribosomal protein L15b|Schizos... 28 0.51
SPCC576.11 |rpl15||60S ribosomal protein L15|Schizosaccharomyces... 28 0.68
SPBC365.14c |||UDP-glucose 4-epimerase |Schizosaccharomyces pomb... 27 1.6
SPAC1093.04c |||tRNA nucleotidyltransferase |Schizosaccharomyces... 26 2.1
SPAC959.09c |apc5|SPAP32A8.01c|anaphase-promoting complex subuni... 25 6.3
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 25 6.3
SPBC530.01 |gyp1||GTPase activating protein Gyp1 |Schizosaccharo... 24 8.4
SPBC1921.05 |ape2||aminopeptidase Ape2|Schizosaccharomyces pombe... 24 8.4
SPCC1919.04 |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 24 8.4
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 139 bits (336), Expect = 2e-34
Identities = 71/140 (50%), Positives = 97/140 (69%), Gaps = 3/140 (2%)
Frame = +3
Query: 3 FTAWCNSHLRKAGT-GIENIEDDFRNGLKLMLLLEVISGETLPKPDRG-KMRFHKIANVN 176
FT W N+ L + ++ D +G+ L+ LLE+I E L + +R +MR H++ NVN
Sbjct: 14 FTKWFNTKLSSRDLPSVFDLRKDLSDGILLIQLLEIIGDENLGRYNRNPRMRVHRLENVN 73
Query: 177 KALDFIASKGVKLVSIGAEEIVDGNLKMTLGMIWTIILRFAIQDISVEEMTAKEGLLLWC 356
KAL++I SKG+ L +IG +IVDGNLK+ LG+IWT+ILRF I DI+ E +TAKEGLLLWC
Sbjct: 74 KALEYIKSKGMPLTNIGPADIVDGNLKLILGLIWTLILRFTIADINEEGLTAKEGLLLWC 133
Query: 357 QRKTAPY-KNVNVQNLHLSF 413
QRKTA Y V+VQ+ S+
Sbjct: 134 QRKTANYHPEVDVQDFTRSW 153
>SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 614
Score = 46.0 bits (104), Expect = 2e-06
Identities = 30/103 (29%), Positives = 49/103 (47%), Gaps = 8/103 (7%)
Frame = +3
Query: 3 FTAWCNSHLRKAGTGIENIEDDFRNGLKLMLLLEVISGETL--------PKPDRGKMRFH 158
FT W NS I + ++ R+GL L+ + I+ T+ P MRF
Sbjct: 391 FTLWLNS--LDVTPSIHDFFNNLRDGLILLQAYDKITPNTVNWKKVNKAPASGDEMMRFK 448
Query: 159 KIANVNKALDFIASKGVKLVSIGAEEIVDGNLKMTLGMIWTII 287
+ N N A+D ++G LV I +I DG+ +TL ++W ++
Sbjct: 449 AVENCNYAVDLGKNQGFSLVGIQGADITDGSRTLTLALVWQMM 491
Score = 35.5 bits (78), Expect = 0.003
Identities = 26/93 (27%), Positives = 46/93 (49%), Gaps = 8/93 (8%)
Frame = +3
Query: 63 DDFRNGLKLMLLLEVISGETLPKPDRGKMRFHK-------IANVNKALDFI-ASKGVKLV 218
D ++GL L L+ +T+ + K R +K I N N ++ A G+ +
Sbjct: 147 DQCKDGLILSKLINDSVPDTIDERVLNKQRNNKPLDNFKCIENNNVVINSAKAMGGISIT 206
Query: 219 SIGAEEIVDGNLKMTLGMIWTIILRFAIQDISV 317
+IGA +I++G + LG++W II R + I +
Sbjct: 207 NIGAGDILEGREHLILGLVWQIIRRGLLGKIDI 239
>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1489
Score = 34.7 bits (76), Expect = 0.006
Identities = 27/98 (27%), Positives = 48/98 (48%), Gaps = 3/98 (3%)
Frame = +3
Query: 60 EDDFRNGLKLMLLLEVISGETLPKPD-RGKMRFHKIANVNKALDFIASKGV-KLVSIGAE 233
E RNG+ L LL++ + L K +++F N+NK LDFI G+ ++
Sbjct: 66 EQSLRNGVVLALLVQKFQPDKLIKIFYSNELQFRHSDNINKFLDFIHGIGLPEIFHFELT 125
Query: 234 EIVDG-NLKMTLGMIWTIILRFAIQDISVEEMTAKEGL 344
+I +G NL + I + ++QD++ + + E L
Sbjct: 126 DIYEGKNLPKVIYCIHALSYFLSMQDLAPPLIKSDENL 163
>SPAC1783.08c |rpl1502|rpl15-2|60S ribosomal protein
L15b|Schizosaccharomyces pombe|chr 1|||Manual
Length = 201
Score = 28.3 bits (60), Expect = 0.51
Identities = 14/51 (27%), Positives = 23/51 (45%), Gaps = 5/51 (9%)
Frame = +2
Query: 5 HSMVQQSPQKSW-----HRHREHRGRLPQWPEAHVAAGGHLRRDSAQARPW 142
H +++ P+ +W H+HRE RG ++ GH +S Q W
Sbjct: 139 HKAIRRDPRINWIVNPVHKHRESRGLTSIGKKSRGIGKGHRYNNSPQHATW 189
>SPCC576.11 |rpl15||60S ribosomal protein L15|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 201
Score = 27.9 bits (59), Expect = 0.68
Identities = 14/51 (27%), Positives = 23/51 (45%), Gaps = 5/51 (9%)
Frame = +2
Query: 5 HSMVQQSPQKSW-----HRHREHRGRLPQWPEAHVAAGGHLRRDSAQARPW 142
H +++ P+ +W H+HRE RG ++ GH +S Q W
Sbjct: 139 HKAIRRDPRINWIVNPVHKHRESRGLTSIGKKSRGIGKGHRFNNSPQHATW 189
>SPBC365.14c |||UDP-glucose 4-epimerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 355
Score = 26.6 bits (56), Expect = 1.6
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = +2
Query: 83 EAHVAAGGHLRRDSAQARPW 142
EAHVAA +LR+ RPW
Sbjct: 250 EAHVAALDYLRQHFVSCRPW 269
>SPAC1093.04c |||tRNA nucleotidyltransferase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 500
Score = 26.2 bits (55), Expect = 2.1
Identities = 22/85 (25%), Positives = 38/85 (44%), Gaps = 7/85 (8%)
Frame = +3
Query: 159 KIANVNKALDFIASKGVKLVSIGAEEIVDGNLKM----TLGMIWTIILRFAI---QDISV 317
K N N+AL I S G+ G EI L+ +L +I I F +D+S+
Sbjct: 242 KHCNTNRALKIIHSLGMFACIFGPLEIHTKKLQSKNIESLSLIPYAIDLFGYLQKKDVSI 301
Query: 318 EEMTAKEGLLLWCQRKTAPYKNVNV 392
+ +++ + W T P+ N ++
Sbjct: 302 KNLSSSSKYIFWLAIATLPWYNWSI 326
>SPAC959.09c |apc5|SPAP32A8.01c|anaphase-promoting complex subunit
Apc5 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 737
Score = 24.6 bits (51), Expect = 6.3
Identities = 12/48 (25%), Positives = 20/48 (41%)
Frame = -1
Query: 184 SALLTLAILWNRILPRSGLGRVSPEMTSSSNMSFRPLRKSSSMFSMPV 41
+A L A+ W RS SPE + + L ++S +P+
Sbjct: 329 TACLNFALAWMFEFKRSQYSNPSPEKNENDDQILEHLARNSQKAQLPI 376
>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1133
Score = 24.6 bits (51), Expect = 6.3
Identities = 17/55 (30%), Positives = 27/55 (49%), Gaps = 1/55 (1%)
Frame = -1
Query: 247 PSTISSAPIDTSFTPFEAMKSS-ALLTLAILWNRILPRSGLGRVSPEMTSSSNMS 86
P + S D S T + + ++ ++ L+I NR + L VSP + SSN S
Sbjct: 118 PDSESQKDTDASLTQMDQLSNTVSVKDLSI--NRNTNKKALNAVSPSLNLSSNSS 170
>SPBC530.01 |gyp1||GTPase activating protein Gyp1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 514
Score = 24.2 bits (50), Expect = 8.4
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = -1
Query: 199 EAMKSSALLTLAILWN 152
++ KSSAL TL+ LWN
Sbjct: 4 QSKKSSALKTLSSLWN 19
>SPBC1921.05 |ape2||aminopeptidase Ape2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 882
Score = 24.2 bits (50), Expect = 8.4
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = -1
Query: 265 RVILRLPSTISSAPIDTSFTPFEAMKSSAL 176
R++L+ PST+ + + PF A SS +
Sbjct: 91 RIVLQFPSTVPANSVAVLTLPFTARISSGM 120
>SPCC1919.04 |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 256
Score = 24.2 bits (50), Expect = 8.4
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = +1
Query: 151 DSTKSLTSTRHSTSSPQKV*SLCQLVPKKSSMV 249
+ TKSL S HS+SS +K + ++ P ++ +V
Sbjct: 198 EKTKSLDSISHSSSSSRKSSTELKIPPVETRIV 230
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,664,615
Number of Sequences: 5004
Number of extensions: 32777
Number of successful extensions: 136
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 126
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 136
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 148351622
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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