Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-1181
         (345 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|ch...   194   4e-51
SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr...   162   2e-41
SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc...    56   2e-09
SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2 |Schizosacc...    41   6e-05
SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces...    27   0.62 
SPBC17G9.06c |||N-acetyltransferase |Schizosaccharomyces pombe|c...    26   1.9  
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    25   3.3  
SPAC144.13c |srw1|ste9|CDK inhibitor Srw1|Schizosaccharomyces po...    24   7.6  
SPAC323.07c |||MatE family transporter|Schizosaccharomyces pombe...    24   7.6  

>SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 778

 Score =  194 bits (472), Expect = 4e-51
 Identities = 89/114 (78%), Positives = 96/114 (84%)
 Frame = +2

Query: 2   KEVYKFLETAGAKYGVGFWKPGSGIIHQIILENYAFPGLLMIGTDSHTPNXXXXXXXXXX 181
           KEVY FL+TA AKY +GFW+PGSGIIHQI+LENYAFPG L+IGTDSHTPN          
Sbjct: 144 KEVYDFLQTACAKYNIGFWRPGSGIIHQIVLENYAFPGGLLIGTDSHTPNAGGLGMVAIG 203

Query: 182 XXXADAVDVMANIPWELKCPKVIGVKLTGKLTGWTSPKDVILKVAGILTVKGGT 343
              ADAVDVMAN+PWELKCPKVIGVKLTG+L GWTSPKDVILKVAGILTVKGGT
Sbjct: 204 VGGADAVDVMANLPWELKCPKVIGVKLTGQLKGWTSPKDVILKVAGILTVKGGT 257


>SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 905

 Score =  162 bits (393), Expect = 2e-41
 Identities = 71/114 (62%), Positives = 86/114 (75%)
 Frame = +2

Query: 2   KEVYKFLETAGAKYGVGFWKPGSGIIHQIILENYAFPGLLMIGTDSHTPNXXXXXXXXXX 181
           KE++ FL++A  KYG+ FW PGSGIIHQI+LENYA PG +M+GTDSHTPN          
Sbjct: 148 KEIFDFLQSAAKKYGIQFWGPGSGIIHQIVLENYAAPGGMMLGTDSHTPNAGGLGMIAIG 207

Query: 182 XXXADAVDVMANIPWELKCPKVIGVKLTGKLTGWTSPKDVILKVAGILTVKGGT 343
              ADAVD M N PWELK PK+IGV LTG ++GWT+PKD+IL +AG LTV+GGT
Sbjct: 208 VGGADAVDAMTNTPWELKAPKIIGVNLTGAMSGWTTPKDLILHLAGKLTVRGGT 261


>SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 721

 Score = 56.0 bits (129), Expect = 2e-09
 Identities = 32/104 (30%), Positives = 49/104 (47%), Gaps = 1/104 (0%)
 Frame = +2

Query: 11  YKFLETAGAKYGVGFWKPGSGIIHQIILEN-YAFPGLLMIGTDSHTPNXXXXXXXXXXXX 187
           YK +E+     G+ F+  G GI HQI++E  YA PG + + +DSH+              
Sbjct: 140 YKNIESFAKGQGIDFYPAGRGIGHQIMVEQGYAMPGSMAVASDSHSNTYGGVGCLGTPIV 199

Query: 188 XADAVDVMANIPWELKCPKVIGVKLTGKLTGWTSPKDVILKVAG 319
             DA  + A      + P +  V L G+L    S KD+I+ + G
Sbjct: 200 RTDAAAIWATGQTWWQIPPIARVNLVGQLPKGLSGKDIIVSLCG 243


>SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 758

 Score = 40.7 bits (91), Expect = 6e-05
 Identities = 25/92 (27%), Positives = 42/92 (45%), Gaps = 1/92 (1%)
 Frame = +2

Query: 71  GIIHQIILEN-YAFPGLLMIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKV 247
           GI+H I  E  +  PG  ++  DSHT               ++   V+A      +  K 
Sbjct: 118 GIVHVIGPEQGFTLPGTTLVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTILQRKSKN 177

Query: 248 IGVKLTGKLTGWTSPKDVILKVAGILTVKGGT 343
           + +++ GKL    + KD+IL + G++   GGT
Sbjct: 178 MRIRVNGKLPEGIASKDLILHIIGVIGTAGGT 209


>SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 404

 Score = 27.5 bits (58), Expect = 0.62
 Identities = 12/26 (46%), Positives = 15/26 (57%)
 Frame = -3

Query: 256 DADNFRTLQLPRDVGHYVHSISTTDT 179
           D+ N    +LP D+ HY H IS  DT
Sbjct: 186 DSQNPSQYRLPDDLVHYFHGISRGDT 211


>SPBC17G9.06c |||N-acetyltransferase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 334

 Score = 25.8 bits (54), Expect = 1.9
 Identities = 9/26 (34%), Positives = 12/26 (46%)
 Frame = +1

Query: 52  FLEARLWYYPSDHSGELCVPWSTHDR 129
           F    +++ P D       PW THDR
Sbjct: 220 FAYFEVYWVPEDRIAPFAQPWHTHDR 245


>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 800

 Score = 25.0 bits (52), Expect = 3.3
 Identities = 11/46 (23%), Positives = 21/46 (45%)
 Frame = -1

Query: 336 PFTVRMPATFKITSFGLVQPVSLPVNLTPITLGHFSSHGMLAITST 199
           P+T     +  +T+       S+P   TP+T  +++      +TST
Sbjct: 480 PYTSTPVTSTPLTTTNCTTSTSVPYTSTPVTSSNYTISSSTPVTST 525



 Score = 24.6 bits (51), Expect = 4.4
 Identities = 15/49 (30%), Positives = 21/49 (42%)
 Frame = -1

Query: 339 PPFTVRMPATFKITSFGLVQPVSLPVNLTPITLGHFSSHGMLAITSTAS 193
           PP T   P+TF  +        S+P N T +     SS   +   ST+S
Sbjct: 692 PPPTTSFPSTFTTSFITSSSLSSIPNNSTEVKTASTSSGTEIKTASTSS 740


>SPAC144.13c |srw1|ste9|CDK inhibitor Srw1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 556

 Score = 23.8 bits (49), Expect = 7.6
 Identities = 8/30 (26%), Positives = 15/30 (50%)
 Frame = +1

Query: 40  IWSRFLEARLWYYPSDHSGELCVPWSTHDR 129
           +W +F E  L+ + +  +    + WS H R
Sbjct: 400 VWDKFEEKPLYSFHNHIAAVKAITWSPHQR 429


>SPAC323.07c |||MatE family transporter|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 533

 Score = 23.8 bits (49), Expect = 7.6
 Identities = 12/24 (50%), Positives = 16/24 (66%)
 Frame = -1

Query: 261 NLTPITLGHFSSHGMLAITSTASA 190
           NL+P+   HFS HGML I +  +A
Sbjct: 312 NLSPML--HFSFHGMLMIVTEWAA 333


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,397,783
Number of Sequences: 5004
Number of extensions: 24988
Number of successful extensions: 99
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 90
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 99
length of database: 2,362,478
effective HSP length: 64
effective length of database: 2,042,222
effective search space used: 102111100
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)