Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-1181
(345 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g35830.1 68417.m05090 aconitate hydratase, cytoplasmic / citr... 53 6e-08
At2g05710.1 68415.m00611 aconitate hydratase, cytoplasmic, putat... 50 5e-07
At4g26970.1 68417.m03881 aconitate hydratase, cytoplasmic, putat... 49 1e-06
At4g13430.1 68417.m02096 aconitase family protein / aconitate hy... 45 1e-05
At2g20870.1 68415.m02459 cell wall protein precursor, putative i... 27 4.4
At5g12440.1 68418.m01462 zinc finger (CCCH-type) family protein ... 26 7.7
At4g16420.2 68417.m02485 transcriptional adaptor (ADA2b) identic... 26 7.7
At2g17500.3 68415.m02024 auxin efflux carrier family protein con... 26 7.7
At2g17500.2 68415.m02023 auxin efflux carrier family protein con... 26 7.7
At2g17500.1 68415.m02022 auxin efflux carrier family protein con... 26 7.7
>At4g35830.1 68417.m05090 aconitate hydratase, cytoplasmic / citrate
hydro-lyase / aconitase (ACO) identical to SP|Q42560
Aconitate hydratase, cytoplasmic (EC 4.2.1.3) (Citrate
hydro-lyase) (Aconitase) {Arabidopsis thaliana};
contains Pfam profiles PF00330: Aconitase family
(aconitate hydratase), PF00694: Aconitase C-terminal
domain
Length = 898
Score = 52.8 bits (121), Expect = 6e-08
Identities = 41/122 (33%), Positives = 57/122 (46%), Gaps = 10/122 (8%)
Frame = +2
Query: 2 KEVYKFLET-AGAKYGVGFWKPGSGIIHQIILENYA-----FPGLL----MIGTDSHTPN 151
KE + FL+ + A + + PGSGI+HQ+ LE A GLL ++GTDSHT
Sbjct: 155 KERFAFLKWGSNAFHNMLVVPPGSGIVHQVNLEYLARVVFNTNGLLYPDSVVGTDSHTTM 214
Query: 152 XXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVKLTGKLTGWTSPKDVILKVAGILTV 331
+A M P + P V+G KLTGKL + D++L V +L
Sbjct: 215 IDGLGVAGWGVGGIEAEATMLGQPMSMVLPGVVGFKLTGKLRDGMTATDLVLTVTQMLRK 274
Query: 332 KG 337
G
Sbjct: 275 HG 276
>At2g05710.1 68415.m00611 aconitate hydratase, cytoplasmic, putative
/ citrate hydro-lyase/aconitase, putative nearly
identical to SP|P49608 Aconitate hydratase, cytoplasmic
(EC 4.2.1.3) (Citrate hydro-lyase) (Aconitase)
{Cucurbita maxima}; contains Pfam profiles PF00330:
Aconitase family (aconitate hydratase), PF00694:
Aconitase C-terminal domain
Length = 990
Score = 49.6 bits (113), Expect = 5e-07
Identities = 37/122 (30%), Positives = 54/122 (44%), Gaps = 10/122 (8%)
Frame = +2
Query: 2 KEVYKFLETAGAKY-GVGFWKPGSGIIHQIILEN-----YAFPGLL----MIGTDSHTPN 151
KE + FL+ + + PGSGI+HQ+ LE + GLL ++GTDSHT
Sbjct: 247 KERFAFLKWGSTAFQNMLVVPPGSGIVHQVNLEYLGRVVFNTKGLLYPDSVVGTDSHTTM 306
Query: 152 XXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVKLTGKLTGWTSPKDVILKVAGILTV 331
+A M P + P V+G KL GK+ + D++L V +L
Sbjct: 307 IDGLGVAGWGVGGIEAEATMLGQPMSMVLPGVVGFKLAGKMRNGVTATDLVLTVTQMLRK 366
Query: 332 KG 337
G
Sbjct: 367 HG 368
>At4g26970.1 68417.m03881 aconitate hydratase, cytoplasmic, putative
/ citrate hydro-lyase/aconitase, putative strong
similarity to SP|P49608 Aconitate hydratase, cytoplasmic
(EC 4.2.1.3) (Citrate hydro-lyase) (Aconitase)
{Cucurbita maxima}; contains Pfam profiles PF00330:
Aconitase family (aconitate hydratase), PF00694:
Aconitase C-terminal domain
Length = 995
Score = 48.8 bits (111), Expect = 1e-06
Identities = 38/122 (31%), Positives = 53/122 (43%), Gaps = 10/122 (8%)
Frame = +2
Query: 2 KEVYKFLETAGAKY-GVGFWKPGSGIIHQIILEN-----YAFPGLL----MIGTDSHTPN 151
KE + FL+ + + PGSGI+HQ+ LE + G L ++GTDSHT
Sbjct: 252 KERFTFLKWGSTAFQNMLVVPPGSGIVHQVNLEYLGRVVFNSKGFLYPDSVVGTDSHTTM 311
Query: 152 XXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVKLTGKLTGWTSPKDVILKVAGILTV 331
+A M P + P V+G KL GKL + D++L V IL
Sbjct: 312 IDGLGVAGWGVGGIEAEAAMLGQPMSMVLPGVVGFKLDGKLKEGVTATDLVLTVTQILRK 371
Query: 332 KG 337
G
Sbjct: 372 HG 373
>At4g13430.1 68417.m02096 aconitase family protein / aconitate
hydratase family protein contains Pfam profile PF00330:
Aconitase family (aconitate hydratase
Length = 509
Score = 45.2 bits (102), Expect = 1e-05
Identities = 27/92 (29%), Positives = 43/92 (46%), Gaps = 1/92 (1%)
Frame = +2
Query: 71 GIIHQIIL-ENYAFPGLLMIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKV 247
G+ H + E + PG +++GTDSHT DA V+ LK P
Sbjct: 177 GVCHVALAQEGHCRPGEVLLGTDSHTCTAGAFGQFATGIGNTDAGFVLGTGKILLKVPPT 236
Query: 248 IGVKLTGKLTGWTSPKDVILKVAGILTVKGGT 343
+ L G++ + KD+IL++ G ++V G T
Sbjct: 237 MRFILDGEMPSYLQAKDLILQIIGEISVAGAT 268
>At2g20870.1 68415.m02459 cell wall protein precursor, putative
identical to Putative cell wall protein precursor
(Swiss-Prot:P47925) [Arabidopsis thaliana]; weak
similarity to mu-protocadherin (GI:7861967) [Rattus
norvegicus]
Length = 140
Score = 26.6 bits (56), Expect = 4.4
Identities = 8/21 (38%), Positives = 12/21 (57%)
Frame = -1
Query: 78 IIPEPGFQKPTPYLAPAVSKN 16
++P PGF+ PTP + N
Sbjct: 111 LVPNPGFEAPTPTIGAGTGSN 131
>At5g12440.1 68418.m01462 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 552
Score = 25.8 bits (54), Expect = 7.7
Identities = 18/59 (30%), Positives = 29/59 (49%)
Frame = -3
Query: 256 DADNFRTLQLPRDVGHYVHSISTTDTNTQSTKATSIRCVGVCADHE*TRERIVLQNDLM 80
D D+ T++L + + ST D T + T+ + VGV + +E E V N+LM
Sbjct: 494 DPDSGSTIELVLPSNLFPSASSTDDHKTDDSAETNAK-VGVSSTNENDHEPPVTTNNLM 551
>At4g16420.2 68417.m02485 transcriptional adaptor (ADA2b) identical
to transcriptional adaptor ADA2b [Arabidopsis thaliana]
gi|13591700|gb|AAK31320
Length = 483
Score = 25.8 bits (54), Expect = 7.7
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = -1
Query: 345 PVPPFTVRMPATFKITSFGLVQPVSLPVNLTPITLGHFS 229
P P V++ T K SFG +PVS VN + + L +++
Sbjct: 192 PFSPPKVKVEDTQKDRSFGGKKPVSTSVNNSLVELSNYN 230
>At2g17500.3 68415.m02024 auxin efflux carrier family protein
contains auxin efflux carrier domain, Pfam:PF03547
Length = 396
Score = 25.8 bits (54), Expect = 7.7
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = +2
Query: 23 ETAGAKYGVGFWKPGSGIIHQIILENYAFPGL 118
E K GFW+ G +H+I+ E A P L
Sbjct: 209 ENKVVKEKTGFWRKGVDFLHEILEELLAPPTL 240
>At2g17500.2 68415.m02023 auxin efflux carrier family protein
contains auxin efflux carrier domain, Pfam:PF03547
Length = 396
Score = 25.8 bits (54), Expect = 7.7
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = +2
Query: 23 ETAGAKYGVGFWKPGSGIIHQIILENYAFPGL 118
E K GFW+ G +H+I+ E A P L
Sbjct: 209 ENKVVKEKTGFWRKGVDFLHEILEELLAPPTL 240
>At2g17500.1 68415.m02022 auxin efflux carrier family protein
contains auxin efflux carrier domain, Pfam:PF03547
Length = 396
Score = 25.8 bits (54), Expect = 7.7
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = +2
Query: 23 ETAGAKYGVGFWKPGSGIIHQIILENYAFPGL 118
E K GFW+ G +H+I+ E A P L
Sbjct: 209 ENKVVKEKTGFWRKGVDFLHEILEELLAPPTL 240
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,431,988
Number of Sequences: 28952
Number of extensions: 135468
Number of successful extensions: 359
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 349
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 356
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 419412672
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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