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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-1139
         (664 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g17920.1 68418.m02101 5-methyltetrahydropteroyltriglutamate--...    30   1.2  
At4g13620.1 68417.m02120 AP2 domain-containing transcription fac...    29   2.8  
At3g26085.1 68416.m03248 CAAX amino terminal protease family pro...    29   2.8  
At3g03780.2 68416.m00387 5-methyltetrahydropteroyltriglutamate--...    29   3.6  
At3g03780.1 68416.m00386 5-methyltetrahydropteroyltriglutamate--...    29   3.6  
At5g39840.1 68418.m04828 ATP-dependent RNA helicase, mitochondri...    28   4.8  
At3g54860.1 68416.m06078 vacuolar protein sorting protein, putat...    28   4.8  
At3g09040.1 68416.m01063 pentatricopeptide (PPR) repeat-containi...    28   4.8  

>At5g17920.1 68418.m02101
           5-methyltetrahydropteroyltriglutamate--homocysteine
           methyltransferase / vitamin-B12-independent methionine
           synthase / cobalamin-independent methionine synthase
           (CIMS) identical to SP|O50008
           5-methyltetrahydropteroyltriglutamate--homocysteine
           methyltransferase (EC 2.1.1.14) (Vitamin-B12-independent
           methionine synthase isozyme) (Cobalamin-independent
           methionine synthase isozyme) {Arabidopsis thaliana}
          Length = 765

 Score = 30.3 bits (65), Expect = 1.2
 Identities = 20/70 (28%), Positives = 38/70 (54%), Gaps = 4/70 (5%)
 Frame = +1

Query: 205 KIKKYLPHSYFL-LLESYLSDRRFQVKLRDARSS---LFACRAGVPQGSVLGPVLYNIFT 372
           +I  ++ +S+F  ++ S +      + + ++RS    L   R GV  G+ +GP +Y+I +
Sbjct: 643 QIHTHMCYSHFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHS 702

Query: 373 SDLPQSPEIA 402
             +P S EIA
Sbjct: 703 PRIPSSEEIA 712


>At4g13620.1 68417.m02120 AP2 domain-containing transcription
           factor, putative similar to AP2 domain containing
           protein RAP2.4 [Arabidopsis thaliana] GI:2281633;
           contains Pfam profile PF00847: AP2 domain
          Length = 388

 Score = 29.1 bits (62), Expect = 2.8
 Identities = 16/31 (51%), Positives = 18/31 (58%)
 Frame = +2

Query: 269 GFKSSSVMPDLLSLPAEPECPKAPYLVRSST 361
           GF SSS  P LL +P   E  K+P L  SST
Sbjct: 69  GFLSSSTPPQLLGVPIFLEGMKSPLLPASST 99


>At3g26085.1 68416.m03248 CAAX amino terminal protease family
           protein contains Pfam profile PF02517 CAAX amino
           terminal protease family protein
          Length = 293

 Score = 29.1 bits (62), Expect = 2.8
 Identities = 14/34 (41%), Positives = 21/34 (61%)
 Frame = +1

Query: 154 LDVQQAFDRVWHRGLLCKIKKYLPHSYFLLLESY 255
           +DV   F+  WH GL+  I  ++  S FLLL+S+
Sbjct: 139 IDVPFGFE-TWHLGLIAGIVVFISSSRFLLLKSW 171


>At3g03780.2 68416.m00387
           5-methyltetrahydropteroyltriglutamate--homocysteine
           methyltransferase, putative / vitamin-B12-independent
           methionine synthase, putative / cobalamin-independent
           methionine synthase, putative very strong similarity to
           SP|O50008
           5-methyltetrahydropteroyltriglutamate--homocysteine
           methyltransferase (EC 2.1.1.14) (Vitamin-B12-independent
           methionine synthase isozyme) (Cobalamin-independent
           methionine synthase isozyme) {Arabidopsis thaliana};
           contains Pfam profile PF01717: Methionine synthase,
           vitamin-B12 independent
          Length = 765

 Score = 28.7 bits (61), Expect = 3.6
 Identities = 14/39 (35%), Positives = 21/39 (53%)
 Frame = +1

Query: 286 RDARSSLFACRAGVPQGSVLGPVLYNIFTSDLPQSPEIA 402
           R     L   R GV  G+ +GP +Y+I +  +P + EIA
Sbjct: 674 RSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTDEIA 712


>At3g03780.1 68416.m00386
           5-methyltetrahydropteroyltriglutamate--homocysteine
           methyltransferase, putative / vitamin-B12-independent
           methionine synthase, putative / cobalamin-independent
           methionine synthase, putative very strong similarity to
           SP|O50008
           5-methyltetrahydropteroyltriglutamate--homocysteine
           methyltransferase (EC 2.1.1.14) (Vitamin-B12-independent
           methionine synthase isozyme) (Cobalamin-independent
           methionine synthase isozyme) {Arabidopsis thaliana};
           contains Pfam profile PF01717: Methionine synthase,
           vitamin-B12 independent
          Length = 765

 Score = 28.7 bits (61), Expect = 3.6
 Identities = 14/39 (35%), Positives = 21/39 (53%)
 Frame = +1

Query: 286 RDARSSLFACRAGVPQGSVLGPVLYNIFTSDLPQSPEIA 402
           R     L   R GV  G+ +GP +Y+I +  +P + EIA
Sbjct: 674 RSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTDEIA 712


>At5g39840.1 68418.m04828 ATP-dependent RNA helicase, mitochondrial,
           putative similar to mitochondrial RNA helicase
           [Arabidopsis thaliana] GI:5823579; contains Pfam profile
           PF00271: Helicase conserved C-terminal domain
          Length = 776

 Score = 28.3 bits (60), Expect = 4.8
 Identities = 20/75 (26%), Positives = 36/75 (48%), Gaps = 9/75 (12%)
 Frame = +1

Query: 94  RVYCTIRNCLERKE--YCSAAFLDVQQAFDRVWHRGLLCKI-----KKYLPHSYFL--LL 246
           + Y  ++  +E K   YCS   L   + FD+V   G+ C +     KKY+P +  +   +
Sbjct: 287 KTYNALQRFMEAKNGLYCSPLRLLAMEVFDKVNALGIYCSLLTGQEKKYVPFANHVSCTV 346

Query: 247 ESYLSDRRFQVKLRD 291
           E   +D  ++V + D
Sbjct: 347 EMVSTDELYEVAVLD 361


>At3g54860.1 68416.m06078 vacuolar protein sorting protein, putative
           similar to Swiss-Prot:Q63615 vacuolar protein sorting
           33A (r-vps33a) [Rattus norvegicus]; contains Pfam
           domain, PF00995: Sec1 family
          Length = 592

 Score = 28.3 bits (60), Expect = 4.8
 Identities = 16/42 (38%), Positives = 22/42 (52%), Gaps = 1/42 (2%)
 Frame = +2

Query: 248 SLISVTEGFKSSSV-MPDLLSLPAEPECPKAPYLVRSSTTFL 370
           SLI  T   K   + +  L + P + EC K  YLVRS  +F+
Sbjct: 49  SLIIPTSKLKELGLELRHLTAEPVQTECTKVVYLVRSQLSFM 90


>At3g09040.1 68416.m01063 pentatricopeptide (PPR) repeat-containing
           protein contains INTERPRO:IPR002885 PPR repeats
          Length = 1028

 Score = 28.3 bits (60), Expect = 4.8
 Identities = 12/38 (31%), Positives = 22/38 (57%), Gaps = 1/38 (2%)
 Frame = +1

Query: 73  STIEQVHRVYCT-IRNCLERKEYCSAAFLDVQQAFDRV 183
           + +E   +++C+ I+  LER  YC  A +D+    DR+
Sbjct: 174 TNVEFGRQIHCSMIKMGLERNSYCGGALVDMYAKCDRI 211


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,956,070
Number of Sequences: 28952
Number of extensions: 340676
Number of successful extensions: 919
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 887
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 919
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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