Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-1135
         (610 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual      47   2e-06
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar...    44   1e-05
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom...    40   4e-04
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo...    38   9e-04
SPAC1F7.11c |||transcription factor zf-fungal binuclear cluster ...    28   0.93 
SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual          28   1.2  
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual    27   2.1  
SPCC31H12.08c |ccr4|SPCC5E4.02c|CCR4-Not complex subunit Ccr4 |S...    26   3.7  
SPBC19C7.08c |||leucine carboxyl methyltransferase|Schizosacchar...    26   5.0  
SPAC1486.02c |ucp14||UBA domain protein Ucp14|Schizosaccharomyce...    25   8.7  

>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 438

 Score = 46.8 bits (106), Expect = 2e-06
 Identities = 20/62 (32%), Positives = 34/62 (54%)
 Frame = +3

Query: 96  KGCPRCGGVVFAAEQVLAKGREWHRKCFKCRDCTKTLDSIIACDGPDGEVYCKTCYGKKW 275
           K C  CGG + A   + A G++ H +CFKC  C++ L+  +     +G+ YC   Y +++
Sbjct: 256 KSCHSCGGSLRAGRIISASGKKLHPQCFKCDTCSQNLEH-VGFYYREGKFYCHLDYHEQF 314

Query: 276 GP 281
            P
Sbjct: 315 SP 316



 Score = 34.3 bits (75), Expect = 0.014
 Identities = 17/63 (26%), Positives = 28/63 (44%)
 Frame = +3

Query: 405 QGCPRCGGMVFAAEQQLAKGTMWHKKCFNCAECHRPLDSMLACDGPDKEIHCRACYAKLF 584
           + C  CGG + A     A G   H +CF C  C + L+  +     + + +C   Y + F
Sbjct: 256 KSCHSCGGSLRAGRIISASGKKLHPQCFKCDTCSQNLEH-VGFYYREGKFYCHLDYHEQF 314

Query: 585 GPK 593
            P+
Sbjct: 315 SPR 317



 Score = 30.7 bits (66), Expect = 0.17
 Identities = 17/59 (28%), Positives = 25/59 (42%), Gaps = 2/59 (3%)
 Frame = +3

Query: 105 PRCGGVVFAAE-QVLAKGREW-HRKCFKCRDCTKTLDSIIACDGPDGEVYCKTCYGKKW 275
           PRC       E Q +    +W H     C  C++  +  I C   D   +C+TCY  K+
Sbjct: 316 PRCKHCKTPIEDQAVHINNDWFHENHHFCAGCSEVFNVNIPCIYRDDLYWCQTCYDNKY 374


>SPBC28E12.03 |rga4||GTPase activating protein
           Rga4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 933

 Score = 44.4 bits (100), Expect = 1e-05
 Identities = 24/73 (32%), Positives = 35/73 (47%), Gaps = 4/73 (5%)
 Frame = +3

Query: 54  TTVIDTASIKAPPGKGC--PRCGGVVFAAEQVLAKGREWHRKCFKCRDCTKTLDSIIACD 227
           T  +D  S++ P  + C   +C   V +  QV   G+ WH  CFKC +C K LD      
Sbjct: 6   TLHLDRLSLETPSERTCFCIKCWESVPSTSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDF 65

Query: 228 GPDG--EVYCKTC 260
             D   +++CK C
Sbjct: 66  SQDDQKQIFCKLC 78



 Score = 39.9 bits (89), Expect = 3e-04
 Identities = 21/69 (30%), Positives = 30/69 (43%), Gaps = 4/69 (5%)
 Frame = +3

Query: 375 DTTSIKAPKGQGC--PRCGGMVFAAEQQLAKGTMWHKKCFNCAECHRPLDSMLACDGPD- 545
           D  S++ P  + C   +C   V +  Q    G  WH  CF C  C++ LD        D 
Sbjct: 10  DRLSLETPSERTCFCIKCWESVPSTSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDD 69

Query: 546 -KEIHCRAC 569
            K+I C+ C
Sbjct: 70  QKQIFCKLC 78


>SPBC3F6.05 |rga1||GTPase activating protein
           Rga1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1150

 Score = 39.5 bits (88), Expect = 4e-04
 Identities = 19/53 (35%), Positives = 27/53 (50%)
 Frame = +3

Query: 54  TTVIDTASIKAPPGKGCPRCGGVVFAAEQVLAKGREWHRKCFKCRDCTKTLDS 212
           T+V   A  K    K C  CG V+ + + V A G  +H +CF+C DC   + S
Sbjct: 100 TSVFPKAVRKVSSSKICASCGQVI-SGQYVRALGNIYHLECFRCHDCNSLVAS 151



 Score = 30.7 bits (66), Expect = 0.17
 Identities = 12/39 (30%), Positives = 20/39 (51%)
 Frame = +3

Query: 390 KAPKGQGCPRCGGMVFAAEQQLAKGTMWHKKCFNCAECH 506
           K    + C  CG  V + +   A G ++H +CF C +C+
Sbjct: 109 KVSSSKICASCG-QVISGQYVRALGNIYHLECFRCHDCN 146


>SPAC29A4.11 |rga3||GTPase activating protein
           Rga3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 969

 Score = 38.3 bits (85), Expect = 9e-04
 Identities = 22/66 (33%), Positives = 29/66 (43%), Gaps = 6/66 (9%)
 Frame = +3

Query: 81  KAPPGKG---CPRCGGVVFAAEQVLA-KGREWHRKCFKCRDCTKTLD--SIIACDGPDGE 242
           K+P  KG   C RCG      E  ++  G  WH+ CF C  C K L+    +     DG 
Sbjct: 9   KSPSSKGSTVCFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGR 68

Query: 243 VYCKTC 260
             C +C
Sbjct: 69  PVCSSC 74



 Score = 38.3 bits (85), Expect = 9e-04
 Identities = 23/67 (34%), Positives = 32/67 (47%), Gaps = 7/67 (10%)
 Frame = +3

Query: 390 KAPKGQG---CPRCGGMVFAAEQQLA-KGTMWHKKCFNCAECHRPL---DSMLACDGPDK 548
           K+P  +G   C RCG      E  ++  G MWHK CF C +C + L   D ML      +
Sbjct: 9   KSPSSKGSTVCFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGR 68

Query: 549 EIHCRAC 569
            + C +C
Sbjct: 69  PV-CSSC 74



 Score = 31.5 bits (68), Expect = 0.100
 Identities = 13/34 (38%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
 Frame = +3

Query: 162 WHRKCFKCRDCTK-TLDSIIACDGPDGEVYCKTC 260
           +HR+CF+C DC K  +DS    D  +  ++C  C
Sbjct: 97  YHRECFRCHDCRKQIIDSNFKRD--NRTIFCNDC 128



 Score = 26.6 bits (56), Expect = 2.8
 Identities = 11/34 (32%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
 Frame = +3

Query: 471 WHKKCFNCAECHRP-LDSMLACDGPDKEIHCRAC 569
           +H++CF C +C +  +DS    D  ++ I C  C
Sbjct: 97  YHRECFRCHDCRKQIIDSNFKRD--NRTIFCNDC 128


>SPAC1F7.11c |||transcription factor zf-fungal binuclear cluster
           type |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 782

 Score = 28.3 bits (60), Expect = 0.93
 Identities = 10/17 (58%), Positives = 12/17 (70%)
 Frame = +3

Query: 459 KGTMWHKKCFNCAECHR 509
           KGT  H+K  +C ECHR
Sbjct: 11  KGTKRHRKITSCRECHR 27


>SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 674

 Score = 27.9 bits (59), Expect = 1.2
 Identities = 19/46 (41%), Positives = 24/46 (52%), Gaps = 3/46 (6%)
 Frame = -1

Query: 508 R*HSAQLK-HFLCHIVP-LANCCSAAKTIPPQRGQPW-PFGALMLV 380
           R H+ +LK HF  + VP L N     +TIPP    PW P G   +V
Sbjct: 67  RHHAFELKNHFEKYYVPSLKNVVHEGQTIPPPTVLPWYPDGLAYIV 112


>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1828

 Score = 27.1 bits (57), Expect = 2.1
 Identities = 13/40 (32%), Positives = 22/40 (55%)
 Frame = -2

Query: 348  ISLPQLSHRFARSQSRKRIRNHEVPISFRSKFCNRLRHQV 229
            ISLP +SH+F ++QS+ +           SK  + LR ++
Sbjct: 1216 ISLPGISHKFQKNQSKTKALGENTGFRKGSKRLDYLRERL 1255


>SPCC31H12.08c |ccr4|SPCC5E4.02c|CCR4-Not complex subunit Ccr4
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 690

 Score = 26.2 bits (55), Expect = 3.7
 Identities = 13/26 (50%), Positives = 15/26 (57%)
 Frame = -3

Query: 137 FSSKHNSTATRAALARRGFNGCSINH 60
           FSS H      AALAR G  G S+N+
Sbjct: 60  FSSPHQRAHNAAALARSGGPGFSMNY 85


>SPBC19C7.08c |||leucine carboxyl
           methyltransferase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 681

 Score = 25.8 bits (54), Expect = 5.0
 Identities = 12/35 (34%), Positives = 19/35 (54%)
 Frame = -3

Query: 170 AMPLAAFGQNLFSSKHNSTATRAALARRGFNGCSI 66
           ++P A+   +  S KHN ++    LA + F GC I
Sbjct: 468 SLPYASHRASSVSIKHNGSSYVVLLAGKPFGGCLI 502


>SPAC1486.02c |ucp14||UBA domain protein Ucp14|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 372

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 11/22 (50%), Positives = 12/22 (54%)
 Frame = +2

Query: 185 PRLHKNFGLHHCLRWT*WRSLL 250
           P  H NFGLH    +  WR LL
Sbjct: 35  PFFHINFGLHLLSHYQYWRILL 56


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,544,405
Number of Sequences: 5004
Number of extensions: 51731
Number of successful extensions: 172
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 268287866
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)