Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-1134
(655 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 38 0.008
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 35 0.054
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 33 0.13
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 33 0.13
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 33 0.17
At2g27100.1 68415.m03256 C2H2 zinc-finger protein SERRATE (SE) i... 32 0.38
At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 30 1.2
At3g49540.1 68416.m05414 expressed protein 30 1.2
At5g55670.1 68418.m06941 RNA recognition motif (RRM)-containing ... 29 2.0
At4g14830.1 68417.m02280 expressed protein 29 2.7
At4g36850.1 68417.m05225 PQ-loop repeat family protein / transme... 29 3.6
At2g28680.1 68415.m03486 cupin family protein similar to legumin... 29 3.6
At5g18190.1 68418.m02135 protein kinase family protein contains ... 28 4.7
At3g56100.1 68416.m06235 leucine-rich repeat transmembrane prote... 28 4.7
At2g20360.1 68415.m02377 expressed protein 28 4.7
At4g17520.1 68417.m02621 nuclear RNA-binding protein, putative s... 28 6.2
At1g76770.1 68414.m08934 heat shock protein-related contains sim... 28 6.2
At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containi... 28 6.2
At5g42820.2 68418.m05216 U2 snRNP auxiliary factor small subunit... 27 8.2
At5g42820.1 68418.m05215 U2 snRNP auxiliary factor small subunit... 27 8.2
At4g19930.1 68417.m02920 F-box family protein similar to F-box p... 27 8.2
At3g56790.1 68416.m06316 RNA splicing factor-related contains we... 27 8.2
At1g65710.1 68414.m07458 expressed protein ; expression supporte... 27 8.2
At1g28060.1 68414.m03435 small nuclear ribonucleoprotein family ... 27 8.2
At1g26140.1 68414.m03191 hypothetical protein 27 8.2
>At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein
(HSP22.0-ER) identical to endomembrane-localized small
heat shock protein GI:511795 from [Arabidopsis thaliana]
Length = 195
Score = 37.5 bits (83), Expect = 0.008
Identities = 20/48 (41%), Positives = 31/48 (64%)
Frame = +3
Query: 396 QFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 539
+F R++ LP+ E+V+++L + GVLTI + P+ VKG R V IA
Sbjct: 132 KFWRQFKLPDNVDMESVKAKLEN-GVLTINLTKLSPEKVKGPRVVNIA 178
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 34.7 bits (76), Expect = 0.054
Identities = 18/50 (36%), Positives = 31/50 (62%)
Frame = +3
Query: 390 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 539
S +F RR+ LPE A E +++ + +GVL++T P KVP+ + + I+
Sbjct: 109 SGKFTRRFRLPENAKMEEIKASM-ENGVLSVTVP-KVPEKKPEVKSIDIS 156
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 33.5 bits (73), Expect = 0.13
Identities = 18/50 (36%), Positives = 30/50 (60%)
Frame = +3
Query: 390 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 539
S +F+RR+ LPE A E V++ + +GVLT+ P K P+ + + I+
Sbjct: 111 SGKFMRRFRLPENAKMEEVKATM-ENGVLTVVVP-KAPEKKPQVKSIDIS 158
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 33.5 bits (73), Expect = 0.13
Identities = 19/50 (38%), Positives = 32/50 (64%)
Frame = +3
Query: 390 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 539
S +F+RR+ LPE A E V++ + +GVL++T P KV ++ + V I+
Sbjct: 108 SGKFMRRFRLPENAKVEEVKASM-ENGVLSVTVP-KVQESKPEVKSVDIS 155
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 33.1 bits (72), Expect = 0.17
Identities = 15/35 (42%), Positives = 23/35 (65%)
Frame = +3
Query: 390 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPR 494
S QF RR+ LPE + V++ + +GVLT+T P+
Sbjct: 105 SGQFTRRFRLPENVKMDQVKAAM-ENGVLTVTVPK 138
>At2g27100.1 68415.m03256 C2H2 zinc-finger protein SERRATE (SE)
identical to C2H2 zinc-finger protein SERRATE
GI:14486602 from [Arabidopsis thaliana]
Length = 720
Score = 31.9 bits (69), Expect = 0.38
Identities = 15/32 (46%), Positives = 16/32 (50%)
Frame = +2
Query: 497 GTRRRQGREKGAHRTDRSRSQGDQGPERGNPG 592
G R R GR G R DRS Q + GNPG
Sbjct: 582 GRRERDGRANGNDRNDRSEDQQRGDNDGGNPG 613
>At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock
protein (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family: identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 134
Score = 30.3 bits (65), Expect = 1.2
Identities = 18/79 (22%), Positives = 33/79 (41%)
Frame = +3
Query: 264 DKFQVNLDVQHFSPEEISVKTADGYIVVXXXXXXXXXXXXYISRQFVRRYALPEGAAPET 443
D ++D+ EEI V+ D ++ + F R++ LPE
Sbjct: 35 DSHTFSVDLPGLRKEEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESIDMIG 94
Query: 444 VESRLSSDGVLTITAPRKV 500
+ + DGVLT+ P+++
Sbjct: 95 ISAGYE-DGVLTVIVPKRI 112
>At3g49540.1 68416.m05414 expressed protein
Length = 166
Score = 30.3 bits (65), Expect = 1.2
Identities = 20/65 (30%), Positives = 35/65 (53%), Gaps = 4/65 (6%)
Frame = +3
Query: 423 EGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKV---PI-AQTGPVRKEIKDQSEGTQ 590
E AA E E+++ + + + AP KV AV+ E+K P+ A+ PV+ E + E ++
Sbjct: 100 ENAATENAEAKVEA---VAVAAPEKVEVAVEAEKKAEAEPVKAEAEPVKAEAEPVKEESK 156
Query: 591 DAENK 605
E +
Sbjct: 157 QEEKE 161
>At5g55670.1 68418.m06941 RNA recognition motif (RRM)-containing
protein
Length = 710
Score = 29.5 bits (63), Expect = 2.0
Identities = 15/39 (38%), Positives = 20/39 (51%), Gaps = 5/39 (12%)
Frame = +2
Query: 464 RRGSHHHRAEEGTR-----RRQGREKGAHRTDRSRSQGD 565
R HHH+ E +R R + RE+ HR +R R GD
Sbjct: 619 REREHHHKDRERSREHVRDRERERERDRHREERERYGGD 657
>At4g14830.1 68417.m02280 expressed protein
Length = 152
Score = 29.1 bits (62), Expect = 2.7
Identities = 13/31 (41%), Positives = 16/31 (51%)
Frame = +3
Query: 402 VRRYALPEGAAPETVESRLSSDGVLTITAPR 494
V R+ LPE PE V DG L +T P+
Sbjct: 109 VWRFRLPESTRPELVTVDCDGDGELIVTVPK 139
>At4g36850.1 68417.m05225 PQ-loop repeat family protein /
transmembrane family protein similar to SP|Q10482 Seven
transmembrane protein 1 {Schizosaccharomyces pombe};
contains Pfam profile PF04193: PQ loop repeat
Length = 374
Score = 28.7 bits (61), Expect = 3.6
Identities = 13/52 (25%), Positives = 30/52 (57%), Gaps = 2/52 (3%)
Frame = -1
Query: 604 LFSASWVPSLWSLISLRTGPVCAMGT--FLSPLTASGTFLGAVMVRTPSDDS 455
++ +P +W ++++ G V + F+ L A+ T++G+++VRT D+
Sbjct: 269 IYMGGRIPQIW--LNIKRGSVEGLNPLMFIFALVANATYVGSILVRTTEWDN 318
>At2g28680.1 68415.m03486 cupin family protein similar to legumin
(11S-globulin) from Ginkgo biloba [GI:949869], 11S
globulin from Avena sativa [GI:472867]; contains a 11-S
plant seed storage protein signature (PS00305)
Length = 356
Score = 28.7 bits (61), Expect = 3.6
Identities = 21/64 (32%), Positives = 35/64 (54%), Gaps = 3/64 (4%)
Frame = +3
Query: 390 SRQFV-RRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERK--VPIAQTGPVRK 560
S +FV R + L E + V S+ + +G++ + A K+P+ KG+RK V P+
Sbjct: 141 STEFVGRAWDLDETTVKKLVGSQ-TGNGIVKVDASLKMPEPKKGDRKGFVLNCLEAPLDV 199
Query: 561 EIKD 572
+IKD
Sbjct: 200 DIKD 203
>At5g18190.1 68418.m02135 protein kinase family protein contains
Pfam domains, PF00069: Protein kinase domain
Length = 691
Score = 28.3 bits (60), Expect = 4.7
Identities = 11/22 (50%), Positives = 12/22 (54%)
Frame = +2
Query: 200 QTVETTRGCIS*PRLQHKGRQG 265
Q T GC P + HKGRQG
Sbjct: 185 QVYNTLNGCYGVPAVHHKGRQG 206
>At3g56100.1 68416.m06235 leucine-rich repeat transmembrane protein
kinase, putative hypothetical proteins - Arabidopsis
thaliana
Length = 719
Score = 28.3 bits (60), Expect = 4.7
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = +1
Query: 469 GFSPSPRRGRYPTPSRERERCPSHR 543
G+S S P+PS E+ER PSHR
Sbjct: 342 GYSVSTPCPTLPSPSPEKERKPSHR 366
>At2g20360.1 68415.m02377 expressed protein
Length = 402
Score = 28.3 bits (60), Expect = 4.7
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = -3
Query: 554 NGTGLCDGHLSLSLDGVGYLPRRGDG 477
NGT C SL+ GVG+L R+G G
Sbjct: 36 NGTDNCRYSSSLATKGVGHLARKGTG 61
>At4g17520.1 68417.m02621 nuclear RNA-binding protein, putative
similar to nuclear RNA binding protein GI:6492264 from
[Arabidopsis thaliana]
Length = 360
Score = 27.9 bits (59), Expect = 6.2
Identities = 15/44 (34%), Positives = 21/44 (47%)
Frame = +2
Query: 470 GSHHHRAEEGTRRRQGREKGAHRTDRSRSQGDQGPERGNPGCRK 601
G + R+EEG R+G G +R DR S + G + RK
Sbjct: 95 GGGYRRSEEGDGARRGGPVGGYRGDRRGSYSNGGDSGDSERPRK 138
>At1g76770.1 68414.m08934 heat shock protein-related contains
similarity to 17.9 kDa heat-shock protein [Helianthus
annuus] gi|11990130|emb|CAB55634
Length = 244
Score = 27.9 bits (59), Expect = 6.2
Identities = 11/37 (29%), Positives = 24/37 (64%), Gaps = 1/37 (2%)
Frame = +3
Query: 393 RQFVRRYALPEGAAPETVESRLSS-DGVLTITAPRKV 500
++F + + +P+ + +++R + DG LT+T P+KV
Sbjct: 93 KEFKKVFRIPDIVILDKIKARFNEEDGTLTVTMPKKV 129
>At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containing
protein low similarity to fertility restorer [Petunia x
hybrida] GI:22128587, post-transcriptional control of
chloroplast gene expression CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 991
Score = 27.9 bits (59), Expect = 6.2
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = -3
Query: 545 GLCDGHLSLSLDGVGYLPRRGDGENPV 465
G C G + L LD + P+ G ++PV
Sbjct: 240 GWCAGKVDLDLDSIDDFPKNGSAQSPV 266
>At5g42820.2 68418.m05216 U2 snRNP auxiliary factor small subunit,
putative strong similarity to U2 snRNP auxiliary factor,
small subunit [Oryza sativa] GI:3850816
Length = 283
Score = 27.5 bits (58), Expect = 8.2
Identities = 19/43 (44%), Positives = 22/43 (51%)
Frame = +1
Query: 400 SSDVTRCLKARRLRLWNRGCHQTGFSPSPRRGRYPTPSRERER 528
S ++ R L R R + RG S SPRR R SRERER
Sbjct: 177 SRELRRKLFGRYRRSYRRGSRSRSRSISPRRKR--EHSRERER 217
>At5g42820.1 68418.m05215 U2 snRNP auxiliary factor small subunit,
putative strong similarity to U2 snRNP auxiliary factor,
small subunit [Oryza sativa] GI:3850816
Length = 283
Score = 27.5 bits (58), Expect = 8.2
Identities = 19/43 (44%), Positives = 22/43 (51%)
Frame = +1
Query: 400 SSDVTRCLKARRLRLWNRGCHQTGFSPSPRRGRYPTPSRERER 528
S ++ R L R R + RG S SPRR R SRERER
Sbjct: 177 SRELRRKLFGRYRRSYRRGSRSRSRSISPRRKR--EHSRERER 217
>At4g19930.1 68417.m02920 F-box family protein similar to F-box
protein family, AtFBX8 (GI:20197464) {Arabidopsis
thaliana}; contains Pfam PF00646: F-box domain
Length = 431
Score = 27.5 bits (58), Expect = 8.2
Identities = 14/52 (26%), Positives = 21/52 (40%), Gaps = 5/52 (9%)
Frame = +1
Query: 514 RERERCPSHRPVPFAXXXXXXXXXXXMP-----KISRVVLQWCILICSKVLS 654
R R+ C SH P+P+ +P + V W LICS+ +
Sbjct: 29 RRRDMCKSHEPMPYIPFDLVIEILTRLPAKSLMRFKSVSKLWSSLICSRTFT 80
>At3g56790.1 68416.m06316 RNA splicing factor-related contains weak
similarity to U4/U6-associated RNA splicing factor [Homo
sapiens] gi|2853287|gb|AAC09069
Length = 206
Score = 27.5 bits (58), Expect = 8.2
Identities = 14/34 (41%), Positives = 18/34 (52%), Gaps = 2/34 (5%)
Frame = +2
Query: 464 RRGSHHHRAEEGTRR--RQGREKGAHRTDRSRSQ 559
R HH R+ EG++R R E GA RT S+
Sbjct: 21 RTKHHHERSHEGSKRKDRSEEENGAARTTNKSSR 54
>At1g65710.1 68414.m07458 expressed protein ; expression supported
by MPSS
Length = 455
Score = 27.5 bits (58), Expect = 8.2
Identities = 16/30 (53%), Positives = 18/30 (60%)
Frame = +1
Query: 448 NRGCHQTGFSPSPRRGRYPTPSRERERCPS 537
+RG + SP RR R TPSRERER S
Sbjct: 155 HRGVERVHGSPRERRRR--TPSRERERSGS 182
>At1g28060.1 68414.m03435 small nuclear ribonucleoprotein family
protein / snRNP family protein contains similarity to
U4/U6 small nuclear ribonucleoprotein hPrp3 [Homo
sapiens] gi|2708307|gb|AAC51926
Length = 786
Score = 27.5 bits (58), Expect = 8.2
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = +2
Query: 464 RRGSHHHRAEEGTRRRQGREKGAHRTDRSRSQG 562
R HH R+ EG++ ++ R K R DR G
Sbjct: 80 REKHHHERSHEGSKEKESRSK---RKDREEENG 109
>At1g26140.1 68414.m03191 hypothetical protein
Length = 162
Score = 27.5 bits (58), Expect = 8.2
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = -3
Query: 227 CSRELSPRSEVVFGEHWTGDG 165
C + L P E V EHW+G+G
Sbjct: 94 CGKTLQPTMEDVKEEHWSGNG 114
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,728,179
Number of Sequences: 28952
Number of extensions: 307706
Number of successful extensions: 1050
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 994
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1049
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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