Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-1086
(694 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing ... 30 1.3
At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing ... 30 1.3
At1g09240.1 68414.m01031 nicotianamine synthase, putative simila... 30 1.7
At3g02330.1 68416.m00216 pentatricopeptide (PPR) repeat-containi... 29 2.2
At1g68790.1 68414.m07863 expressed protein 28 6.8
At1g06260.1 68414.m00662 cysteine proteinase, putative contains ... 28 6.8
At5g64390.2 68418.m08088 KH domain-containing RNA-binding protei... 27 8.9
At5g64390.1 68418.m08089 KH domain-containing RNA-binding protei... 27 8.9
At5g60410.2 68418.m07579 DNA-binding family protein contains Pfa... 27 8.9
At5g60410.1 68418.m07578 DNA-binding family protein contains Pfa... 27 8.9
At1g14130.1 68414.m01670 2-oxoglutarate-dependent dioxygenase, p... 27 8.9
>At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing
protein contains Pfam profile PF01465: GRIP domain;
supporting cDNA gi|20303028|gb|AF499634.1|
Length = 765
Score = 30.3 bits (65), Expect = 1.3
Identities = 26/81 (32%), Positives = 42/81 (51%), Gaps = 3/81 (3%)
Frame = +3
Query: 297 ESKCKEGISDKVPYNPKITNLESLLADISVRYIQ---LKESDFELHYKVLDTIFQNLHKK 467
E+ EGI ++ N K+ N SVR + ++E D E+ +++D + NL K
Sbjct: 555 EASPAEGIEKELE-NAKLRNKRMKEEHESVRELADRLIEEKDREIS-RLVDEM-TNLRKS 611
Query: 468 MKEIDPYYNRYSSMVHHAGSH 530
M E P +N+ S VHH G++
Sbjct: 612 M-ESKPVWNKSPSQVHHYGNN 631
>At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing
protein contains Pfam profile PF01465: GRIP domain;
supporting cDNA gi|20303028|gb|AF499634.1|
Length = 788
Score = 30.3 bits (65), Expect = 1.3
Identities = 26/81 (32%), Positives = 42/81 (51%), Gaps = 3/81 (3%)
Frame = +3
Query: 297 ESKCKEGISDKVPYNPKITNLESLLADISVRYIQ---LKESDFELHYKVLDTIFQNLHKK 467
E+ EGI ++ N K+ N SVR + ++E D E+ +++D + NL K
Sbjct: 555 EASPAEGIEKELE-NAKLRNKRMKEEHESVRELADRLIEEKDREIS-RLVDEM-TNLRKS 611
Query: 468 MKEIDPYYNRYSSMVHHAGSH 530
M E P +N+ S VHH G++
Sbjct: 612 M-ESKPVWNKSPSQVHHYGNN 631
>At1g09240.1 68414.m01031 nicotianamine synthase, putative similar
to nicotianamine synthase [Lycopersicon
esculentum][GI:4753801], nicotianamine synthase 2
[Hordeum vulgare][GI:4894912]
Length = 320
Score = 29.9 bits (64), Expect = 1.7
Identities = 21/69 (30%), Positives = 33/69 (47%), Gaps = 5/69 (7%)
Frame = +3
Query: 309 KEGISDKVPYNPKI--TNLESLLADISVRYIQ---LKESDFELHYKVLDTIFQNLHKKMK 473
K+ +S +P NP I T + + +I + I+ L E E H+ + T +Q+
Sbjct: 36 KQLVSTCIPPNPNIDVTKMCDRVQEIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHL 95
Query: 474 EIDPYYNRY 500
I PYYN Y
Sbjct: 96 NIFPYYNNY 104
>At3g02330.1 68416.m00216 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 861
Score = 29.5 bits (63), Expect = 2.2
Identities = 16/52 (30%), Positives = 28/52 (53%)
Frame = -3
Query: 692 LRGPFSVEHSPACLGSNFISLSFRLFVFLVEAKRHTYFDNHVEFIGFVYTHI 537
+RG F ++ P C +++ L L VFLV K H ++ E +G +Y+ +
Sbjct: 791 MRG-FKLKKEPGC---SWVELKDELHVFLVGDKAHPRWEEIYEELGLIYSEM 838
>At1g68790.1 68414.m07863 expressed protein
Length = 1085
Score = 27.9 bits (59), Expect = 6.8
Identities = 25/82 (30%), Positives = 37/82 (45%), Gaps = 14/82 (17%)
Frame = +3
Query: 273 FNRNSRAPESKCKEGISDKVPYNPKITN------------LESLLADISVRYIQLKESDF 416
FN +R E + +E + D + Y K+ LE ISVR LKE +
Sbjct: 625 FNERARTYEKRSQEEL-DNINYTKKLAQREMEEMQYEKLALEREREQISVRKKLLKEQEA 683
Query: 417 ELHYKV--LDTIFQNLHKKMKE 476
E+H + LD + +L +K KE
Sbjct: 684 EMHKDITELDVLRSSLKEKRKE 705
>At1g06260.1 68414.m00662 cysteine proteinase, putative contains
similarity to thiol-protease, pre-pro-TPE4A protein
GI:3688528 [Pisum sativum]
Length = 343
Score = 27.9 bits (59), Expect = 6.8
Identities = 13/37 (35%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
Frame = +3
Query: 387 RYIQLKESDFELHYKVLDTIFQNLHKKMKEI-DPYYN 494
R+ + S+F+ H+ L+T LHKK + + DP N
Sbjct: 90 RFADMTNSEFKAHFLGLNTSSLRLHKKQRPVCDPAGN 126
>At5g64390.2 68418.m08088 KH domain-containing RNA-binding protein
(HEN4) contains similarity to RNA-binding protein;
identical to cDNA HEN4 isoform 2 (HEN4) GI:28261404;
contains Pfam domain PF00013: KH domain; identical to
cDNA HEN4 (HEN4) GI:28261402
Length = 824
Score = 27.5 bits (58), Expect = 8.9
Identities = 19/75 (25%), Positives = 35/75 (46%), Gaps = 7/75 (9%)
Frame = +3
Query: 327 KVPYNPKITNLESLLADISVRYIQLKESDFELHYKVLDTIFQN-----LHKKMKEI--DP 485
KV NPK + + I+ + ++E+ F + ++ D++F N L K + +
Sbjct: 579 KVEQNPKCISENDQVVQITGEFPNVREAIFHITSRLRDSVFSNSMKNSLAKSSSALTTER 638
Query: 486 YYNRYSSMVHHAGSH 530
+Y+R S GSH
Sbjct: 639 FYDRQSDNPLSIGSH 653
>At5g64390.1 68418.m08089 KH domain-containing RNA-binding protein
(HEN4) contains similarity to RNA-binding protein;
identical to cDNA HEN4 isoform 2 (HEN4) GI:28261404;
contains Pfam domain PF00013: KH domain; identical to
cDNA HEN4 (HEN4) GI:28261402
Length = 857
Score = 27.5 bits (58), Expect = 8.9
Identities = 19/75 (25%), Positives = 35/75 (46%), Gaps = 7/75 (9%)
Frame = +3
Query: 327 KVPYNPKITNLESLLADISVRYIQLKESDFELHYKVLDTIFQN-----LHKKMKEI--DP 485
KV NPK + + I+ + ++E+ F + ++ D++F N L K + +
Sbjct: 579 KVEQNPKCISENDQVVQITGEFPNVREAIFHITSRLRDSVFSNSMKNSLAKSSSALTTER 638
Query: 486 YYNRYSSMVHHAGSH 530
+Y+R S GSH
Sbjct: 639 FYDRQSDNPLSIGSH 653
>At5g60410.2 68418.m07579 DNA-binding family protein contains Pfam
profiles: PF02037 SAP domain, PF02891 MIZ zinc finger,
PF00628 PHD-finger
Length = 885
Score = 27.5 bits (58), Expect = 8.9
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +3
Query: 477 IDPYYNRYSSMVHHAGSHYDNVRINKPD 560
+DPY+NR +S + H + + KPD
Sbjct: 416 VDPYFNRITSKMKHCDEEVTEIEV-KPD 442
>At5g60410.1 68418.m07578 DNA-binding family protein contains Pfam
profiles: PF02037 SAP domain, PF02891 MIZ zinc finger,
PF00628 PHD-finger
Length = 873
Score = 27.5 bits (58), Expect = 8.9
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +3
Query: 477 IDPYYNRYSSMVHHAGSHYDNVRINKPD 560
+DPY+NR +S + H + + KPD
Sbjct: 416 VDPYFNRITSKMKHCDEEVTEIEV-KPD 442
>At1g14130.1 68414.m01670 2-oxoglutarate-dependent dioxygenase,
putative similar to adventitious rooting related
oxygenase ARRO-1 from Malus x domestica, gi|3492806;
contains Pfam domain PF03171, 2OG-Fe(II) oxygenase
superfamily
Length = 308
Score = 27.5 bits (58), Expect = 8.9
Identities = 12/43 (27%), Positives = 24/43 (55%)
Frame = +3
Query: 363 SLLADISVRYIQLKESDFELHYKVLDTIFQNLHKKMKEIDPYY 491
SL+A++ I L + +E+ + D + + ++ EI+PYY
Sbjct: 46 SLMAEMKKTVIDLFQRPYEVKVRNTDVLLGSGYRAPNEINPYY 88
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,460,200
Number of Sequences: 28952
Number of extensions: 253414
Number of successful extensions: 684
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 675
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 684
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1477286152
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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