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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-1049
         (686 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g17265.1 68415.m01994 homoserine kinase (HSK) identical to ho...    29   3.8  
At1g79690.1 68414.m09294 MutT/nudix family protein contains Pfam...    28   6.7  
At5g40470.1 68418.m04908 expressed protein                             27   8.8  
At1g65200.1 68414.m07392 ubiquitin carboxyl-terminal hydrolase-r...    27   8.8  

>At2g17265.1 68415.m01994 homoserine kinase (HSK) identical to
           homoserine kinase [Arabidopsis thaliana]
           gi|4927412|gb|AAD33097
          Length = 370

 Score = 28.7 bits (61), Expect = 3.8
 Identities = 18/63 (28%), Positives = 28/63 (44%)
 Frame = -1

Query: 419 IRSQNLESHEHEGAQQGSAVGTTLQNADDATLTAETLFSEIQEQHEAVLAAGLQDPGHAE 240
           IRS  L    H+G   GS +G++  +A  A +    +F       + VL AGL+      
Sbjct: 130 IRSVGLSLDLHKGLPLGSGLGSSAASAAAAAVAVNEIFGRKLGSDQLVL-AGLESEAKVS 188

Query: 239 VHH 231
            +H
Sbjct: 189 GYH 191


>At1g79690.1 68414.m09294 MutT/nudix family protein contains Pfam
           NUDIX domain [PF00293]; very low similarity to Chain A
           and Chain B of Escherichia coli isopentenyl
           diphosphate:dimethylallyl diphosphate isomerase
           [gi:15826361] [gi:15826360]
          Length = 772

 Score = 27.9 bits (59), Expect = 6.7
 Identities = 8/22 (36%), Positives = 14/22 (63%)
 Frame = -3

Query: 72  DSRRCWPKSWSLTIAGHLASRD 7
           D +  WP  W ++ AGH+++ D
Sbjct: 54  DDKDSWPGQWDISSAGHISAGD 75


>At5g40470.1 68418.m04908 expressed protein
          Length = 496

 Score = 27.5 bits (58), Expect = 8.8
 Identities = 11/25 (44%), Positives = 17/25 (68%)
 Frame = -1

Query: 374 QGSAVGTTLQNADDATLTAETLFSE 300
           +G  +GTT   +D+  L AET+FS+
Sbjct: 87  RGVTIGTTPSRSDEEHLKAETIFSD 111


>At1g65200.1 68414.m07392 ubiquitin carboxyl-terminal
            hydrolase-related contains Pfam profiles PF00443:
            Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein
            of unknown function (DUF629), PF04781: Protein of unknown
            function (DUF627)
          Length = 1101

 Score = 27.5 bits (58), Expect = 8.8
 Identities = 27/97 (27%), Positives = 42/97 (43%), Gaps = 5/97 (5%)
 Frame = -1

Query: 395  HEHEGAQQGSAVGTTLQNADDATLTAET----LFSEIQEQHEAVLAAGLQDPGHAEVHHV 228
            HEH    +  +   +L+  ++ TLT+E+    + S I  Q E   A  L        H V
Sbjct: 739  HEHSANLELDSTSPSLKPVEEDTLTSESGLLEMKSNINNQEETTKA--LHGEDSLSKHPV 796

Query: 227  VVH-NSLPAYNCVFSEINLELNFNGVLVLALQDLVHN 120
              H  +   YN +   + L+   N  L +  +DLVHN
Sbjct: 797  SAHEEATTRYNSILDMV-LKALCN--LKVLKEDLVHN 830


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,582,579
Number of Sequences: 28952
Number of extensions: 259640
Number of successful extensions: 578
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 569
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 578
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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