BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-1049 (686 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g17265.1 68415.m01994 homoserine kinase (HSK) identical to ho... 29 3.8 At1g79690.1 68414.m09294 MutT/nudix family protein contains Pfam... 28 6.7 At5g40470.1 68418.m04908 expressed protein 27 8.8 At1g65200.1 68414.m07392 ubiquitin carboxyl-terminal hydrolase-r... 27 8.8 >At2g17265.1 68415.m01994 homoserine kinase (HSK) identical to homoserine kinase [Arabidopsis thaliana] gi|4927412|gb|AAD33097 Length = 370 Score = 28.7 bits (61), Expect = 3.8 Identities = 18/63 (28%), Positives = 28/63 (44%) Frame = -1 Query: 419 IRSQNLESHEHEGAQQGSAVGTTLQNADDATLTAETLFSEIQEQHEAVLAAGLQDPGHAE 240 IRS L H+G GS +G++ +A A + +F + VL AGL+ Sbjct: 130 IRSVGLSLDLHKGLPLGSGLGSSAASAAAAAVAVNEIFGRKLGSDQLVL-AGLESEAKVS 188 Query: 239 VHH 231 +H Sbjct: 189 GYH 191 >At1g79690.1 68414.m09294 MutT/nudix family protein contains Pfam NUDIX domain [PF00293]; very low similarity to Chain A and Chain B of Escherichia coli isopentenyl diphosphate:dimethylallyl diphosphate isomerase [gi:15826361] [gi:15826360] Length = 772 Score = 27.9 bits (59), Expect = 6.7 Identities = 8/22 (36%), Positives = 14/22 (63%) Frame = -3 Query: 72 DSRRCWPKSWSLTIAGHLASRD 7 D + WP W ++ AGH+++ D Sbjct: 54 DDKDSWPGQWDISSAGHISAGD 75 >At5g40470.1 68418.m04908 expressed protein Length = 496 Score = 27.5 bits (58), Expect = 8.8 Identities = 11/25 (44%), Positives = 17/25 (68%) Frame = -1 Query: 374 QGSAVGTTLQNADDATLTAETLFSE 300 +G +GTT +D+ L AET+FS+ Sbjct: 87 RGVTIGTTPSRSDEEHLKAETIFSD 111 >At1g65200.1 68414.m07392 ubiquitin carboxyl-terminal hydrolase-related contains Pfam profiles PF00443: Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein of unknown function (DUF629), PF04781: Protein of unknown function (DUF627) Length = 1101 Score = 27.5 bits (58), Expect = 8.8 Identities = 27/97 (27%), Positives = 42/97 (43%), Gaps = 5/97 (5%) Frame = -1 Query: 395 HEHEGAQQGSAVGTTLQNADDATLTAET----LFSEIQEQHEAVLAAGLQDPGHAEVHHV 228 HEH + + +L+ ++ TLT+E+ + S I Q E A L H V Sbjct: 739 HEHSANLELDSTSPSLKPVEEDTLTSESGLLEMKSNINNQEETTKA--LHGEDSLSKHPV 796 Query: 227 VVH-NSLPAYNCVFSEINLELNFNGVLVLALQDLVHN 120 H + YN + + L+ N L + +DLVHN Sbjct: 797 SAHEEATTRYNSILDMV-LKALCN--LKVLKEDLVHN 830 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,582,579 Number of Sequences: 28952 Number of extensions: 259640 Number of successful extensions: 578 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 569 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 578 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1457719448 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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