Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-1025
(698 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g63430.1 68416.m07142 expressed protein similarity to predict... 31 0.97
At1g60070.1 68414.m06767 gamma-adaptin, putative similar to gamm... 30 1.7
At1g48970.1 68414.m05489 eukaryotic translation initiation facto... 30 1.7
At3g62900.1 68416.m07066 expressed protein ; expression supporte... 29 2.2
At1g64170.1 68414.m07269 cation/hydrogen exchanger, putative (CH... 29 3.9
At2g43900.1 68415.m05457 endonuclease/exonuclease/phosphatase fa... 28 6.8
At1g15290.1 68414.m01830 tetratricopeptide repeat (TPR)-containi... 28 6.8
At5g08200.1 68418.m00959 peptidoglycan-binding LysM domain-conta... 27 9.0
At4g00030.1 68417.m05681 plastid-lipid associated protein PAP / ... 27 9.0
At3g10350.1 68416.m01241 anion-transporting ATPase family protei... 27 9.0
At1g22190.1 68414.m02775 AP2 domain-containing transcription fac... 27 9.0
>At3g63430.1 68416.m07142 expressed protein similarity to predicted
protein, Arabidopsis thaliana
Length = 540
Score = 30.7 bits (66), Expect = 0.97
Identities = 24/57 (42%), Positives = 34/57 (59%), Gaps = 1/57 (1%)
Frame = +1
Query: 295 SGTQNRKSVHSRTLLTSPAAVIRRSKQSS*ISAKR-QNRKLAPQLTSPTSPAVVQSR 462
SG N++SV+S L SPA +RS SS S++ + K AP+L S S AVV ++
Sbjct: 46 SGRTNQQSVYSTPELRSPAPAQQRSSSSSSSSSRPWRFSKEAPRL-SLDSRAVVDAK 101
>At1g60070.1 68414.m06767 gamma-adaptin, putative similar to
gamma-adaptin GI:2765190 from [Homo sapiens]; contains
Pfam profiles PF01602: Adaptin N terminal region,
PF02883: Adaptin C-terminal domain
Length = 867
Score = 29.9 bits (64), Expect = 1.7
Identities = 14/35 (40%), Positives = 20/35 (57%)
Frame = +1
Query: 343 SPAAVIRRSKQSS*ISAKRQNRKLAPQLTSPTSPA 447
+PA V S +S + N +AP LTSPT+P+
Sbjct: 694 TPAPVQNGSANGDLLSIQDNNAPIAPSLTSPTAPS 728
>At1g48970.1 68414.m05489 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
guanine nucleotide exchange factor, eIF-2B, delta
subunit [Mus musculus] GI:529428; contains Pfam profile
PF01008: Initiation factor 2 subunit family
Length = 756
Score = 29.9 bits (64), Expect = 1.7
Identities = 13/29 (44%), Positives = 18/29 (62%)
Frame = -2
Query: 502 SSDFGSCEAGGSGLLIAPPPGLWVMLAVE 416
+SD +C A G+G+L P G+W M VE
Sbjct: 199 TSDEAACSAVGTGVLGRPYSGIWRMFKVE 227
>At3g62900.1 68416.m07066 expressed protein ; expression supported by
MPSS
Length = 1401
Score = 29.5 bits (63), Expect = 2.2
Identities = 38/164 (23%), Positives = 60/164 (36%)
Frame = +3
Query: 51 PTEKKKVPMNKVQVGNAPSPNLKAVKSKIGSLDNATYKPGGGKVKIENRKLEFGNITPKI 230
PT VP+ + P + K K + G+ D GG I+ RKL + +I
Sbjct: 771 PTSSSGVPITSADIKPKPRVSSKMPKEEGGASDTGNSNSTGG---IKKRKLRESH-GSRI 826
Query: 231 AAKNEAYTPSGGAKKIVTNKLEWNAKSKVGSLQNASYKPGGGDKKIETVKLDFGEKAKSK 410
++NE + KK K E G+ K KIE + A S
Sbjct: 827 YSENENHE----RKKARVRKEEKEPSYSQGN--GKLEKKNRSHSKIEYANVQNSIAATSS 880
Query: 411 VGSTANITHKPGGGAIKSPLPPASQLPKSDENLNEEQS*ITTRK 542
S + +HKP + ++ P + S L+ + I+ K
Sbjct: 881 -SSKISDSHKPRNSSREAKCSPVESVSSSPMRLSNPEKRISVSK 923
>At1g64170.1 68414.m07269 cation/hydrogen exchanger, putative
(CHX16) monovalent cation:proton antiporter family 2
(CPA2) member, PMID:11500563
Length = 847
Score = 28.7 bits (61), Expect = 3.9
Identities = 12/37 (32%), Positives = 23/37 (62%)
Frame = -2
Query: 292 SLLVTIFLAPPLGVYASFLAAILGVMFPNSSFLFSIL 182
++L+ F +G++A F A ++GV+FP F +I+
Sbjct: 317 AVLLAGFATDAIGIHAIFGAFVMGVLFPKGHFSDAIV 353
>At2g43900.1 68415.m05457 endonuclease/exonuclease/phosphatase family
protein belongs to Pfam:PF03372:
Endonuclease/Exonuclease/phosphatase family; contains 3
WD-40 repeats (PF00400);similar to Type II
inositol-1,4,5-trisphosphate 5-phosphatase EC 3.1.3.56
(5PTASE) (INPP5B) (SP:P32019) [Homo sapiens]
Length = 1305
Score = 27.9 bits (59), Expect = 6.8
Identities = 20/90 (22%), Positives = 36/90 (40%)
Frame = +3
Query: 111 NLKAVKSKIGSLDNATYKPGGGKVKIENRKLEFGNITPKIAAKNEAYTPSGGAKKIVTNK 290
N K+ K G + + K G +++K G+ + K KN+ + S KK +
Sbjct: 1193 NSKSSKKSDGDSCSKSQKKSDGDTNSKSQKKGDGDSSSKSHKKNDGDSSSKSHKKNDGDS 1252
Query: 291 LEWNAKSKVGSLQNASYKPGGGDKKIETVK 380
+ K G + S+K GD + +
Sbjct: 1253 SSKSHKKSDGDSSSKSHKKSEGDSSLSLTR 1282
>At1g15290.1 68414.m01830 tetratricopeptide repeat (TPR)-containing
protein ESTs gb|F20110 and gb|F20109 come from this gene;
contains Pfam profile PF00515: TPR Domain
Length = 1558
Score = 27.9 bits (59), Expect = 6.8
Identities = 16/53 (30%), Positives = 23/53 (43%)
Frame = +3
Query: 228 IAAKNEAYTPSGGAKKIVTNKLEWNAKSKVGSLQNASYKPGGGDKKIETVKLD 386
++ K A P G K + KLE N + + S G + K +TV LD
Sbjct: 1255 LSYKEVALAPPGTVLKPMLEKLELNLERTETQIYRTSSASSGEESKSDTVMLD 1307
>At5g08200.1 68418.m00959 peptidoglycan-binding LysM
domain-containing protein contains Pfam profile PF01476:
LysM domain
Length = 409
Score = 27.5 bits (58), Expect = 9.0
Identities = 22/75 (29%), Positives = 33/75 (44%), Gaps = 2/75 (2%)
Frame = +3
Query: 141 SLDNATYKPGGGKVKIENRKLE--FGNITPKIAAKNEAYTPSGGAKKIVTNKLEWNAKSK 314
S DN T +P K +K E F + TP++ K E + +GG I L +SK
Sbjct: 254 SPDNNTQEPTSDKFMRRRQKSEADFSSRTPELVLKEENGSSNGGFLSIAGKGLA--LRSK 311
Query: 315 VGSLQNASYKPGGGD 359
S N + + G+
Sbjct: 312 ASSRTNLAAESENGN 326
>At4g00030.1 68417.m05681 plastid-lipid associated protein PAP /
fibrillin family protein contains Pfam profile PF04755:
PAP_fibrillin
Length = 212
Score = 27.5 bits (58), Expect = 9.0
Identities = 11/44 (25%), Positives = 23/44 (52%)
Frame = -2
Query: 277 IFLAPPLGVYASFLAAILGVMFPNSSFLFSILTLPPPGLYVALS 146
+F+ G++ + +L V+ N L +++T PP G++ S
Sbjct: 99 LFIIEKAGLFGTTAGDVLQVIDVNKRILNNVITFPPDGVFFVRS 142
>At3g10350.1 68416.m01241 anion-transporting ATPase family protein
similar to SP|O43681 Arsenical pump-driving ATPase (EC
3.6.3.16) (Arsenite-translocating ATPase) (Arsenical
resistance ATPase) (Arsenite-transporting ATPase) (ARSA)
(ASNA-I) {Homo sapiens}; contains Pfam profile PF02374:
Anion-transporting ATPase; contains non-consensus GA
donor splice site at intron 5
Length = 411
Score = 27.5 bits (58), Expect = 9.0
Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = -2
Query: 463 LLIAPPPGLWVMLAVEPTFDFAFSPKSSL-TVSIFLSPPPG 344
LL PPPGL +A+ F SP+ ++ T +F + P G
Sbjct: 202 LLDTPPPGLDEAIAISKVIQFLESPEYNMFTRIVFDTAPTG 242
>At1g22190.1 68414.m02775 AP2 domain-containing transcription
factor, putative similar to AP2 domain containing
protein RAP2.4 GI:2281633 from [Arabidopsis thaliana]
Length = 261
Score = 27.5 bits (58), Expect = 9.0
Identities = 16/48 (33%), Positives = 21/48 (43%)
Frame = +3
Query: 327 QNASYKPGGGDKKIETVKLDFGEKAKSKVGSTANITHKPGGGAIKSPL 470
Q S K K+ TV + E+ S GS+ +T G G SPL
Sbjct: 172 QVRSTKKSSSRKRSSTVAVKLPEEDYSSAGSSPLLTESYGSGGSSSPL 219
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,627,982
Number of Sequences: 28952
Number of extensions: 353042
Number of successful extensions: 1007
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 969
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1006
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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