Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-1003
(641 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 44 9e-05
At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 43 2e-04
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 41 6e-04
At5g12030.1 68418.m01406 17.7 kDa class II heat shock protein 17... 39 0.002
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 37 0.010
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 37 0.013
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 34 0.070
At1g78910.1 68414.m09199 pseudouridine synthase family protein l... 29 2.6
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 29 3.5
At5g04290.1 68418.m00422 KOW domain-containing transcription fac... 29 3.5
At3g49540.1 68416.m05414 expressed protein 28 4.6
At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containi... 27 8.0
At1g34180.1 68414.m04239 no apical meristem (NAM) family protein... 27 8.0
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 44.0 bits (99), Expect = 9e-05
Identities = 32/93 (34%), Positives = 50/93 (53%), Gaps = 8/93 (8%)
Frame = +1
Query: 196 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 351
D+ EE+ V+ DG I+ + G+ +EEK D+ + R +FTRR+ LPE E
Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125
Query: 352 SVESRLSSDGVLSVIAPRKVPPAVEGERKIPIA 450
+++ + +GVLSV P KVP + I I+
Sbjct: 126 EIKASM-ENGVLSVTVP-KVPEKKPEVKSIDIS 156
>At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein
(HSP22.0-ER) identical to endomembrane-localized small
heat shock protein GI:511795 from [Arabidopsis thaliana]
Length = 195
Score = 43.2 bits (97), Expect = 2e-04
Identities = 31/104 (29%), Positives = 56/104 (53%), Gaps = 8/104 (7%)
Frame = +1
Query: 163 KSDKDKFQVNLDVQHFAPEEISVKTAD-GYIVVEG---KHEEKK-DQHGYISR---QFTR 318
K + ++ LD+ +E+ ++ + G + V G + EEKK DQ + R +F R
Sbjct: 76 KETAEGHEIMLDIPGLKKDEVKIEVEENGVLRVSGERKREEEKKGDQWHRVERSYGKFWR 135
Query: 319 RYALPEGCTAESVESRLSSDGVLSVIAPRKVPPAVEGERKIPIA 450
++ LP+ ESV+++L +GVL++ + P V+G R + IA
Sbjct: 136 QFKLPDNVDMESVKAKL-ENGVLTINLTKLSPEKVKGPRVVNIA 178
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 41.1 bits (92), Expect = 6e-04
Identities = 28/78 (35%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
Frame = +1
Query: 196 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 351
DV EE+ V+ DG I+ + G+ +EEK D + R +F RR+ LPE E
Sbjct: 65 DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124
Query: 352 SVESRLSSDGVLSVIAPR 405
V++ + +GVLSV P+
Sbjct: 125 EVKASM-ENGVLSVTVPK 141
>At5g12030.1 68418.m01406 17.7 kDa class II heat shock protein 17.6A
(HSP17.7-CII) identical to heat shock protein 17.6A
GI:3256075 from [Arabidopsis thaliana]
Length = 156
Score = 39.1 bits (87), Expect = 0.002
Identities = 34/126 (26%), Positives = 58/126 (46%), Gaps = 9/126 (7%)
Frame = +1
Query: 97 SREYYRPWRHLAAAARDLGSSIKSDKDKFQVNLDVQHFAPEEISVKTA-DGYIVVEGKHE 273
SR Y R + +AA D + D + +D+ +EI V+ + +VV GK +
Sbjct: 33 SRAYMRDAKAMAATPAD----VIEHPDAYVFAVDMPGIKGDEIQVQIENENVLVVSGKRQ 88
Query: 274 -EKKDQHGY----ISRQ---FTRRYALPEGCTAESVESRLSSDGVLSVIAPRKVPPAVEG 429
+ K+ G + R+ F R++ LP+ E + S +DGVL V P+ PP +
Sbjct: 89 RDNKENEGVKFVRMERRMGKFMRKFQLPDNADLEKI-SAACNDGVLKVTIPKLPPPEPKK 147
Query: 430 ERKIPI 447
+ I +
Sbjct: 148 PKTIQV 153
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 37.1 bits (82), Expect = 0.010
Identities = 24/78 (30%), Positives = 43/78 (55%), Gaps = 8/78 (10%)
Frame = +1
Query: 196 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 351
D+ EE+ V+ D ++ + G+ +EEK D+ + R +F RR+ LPE E
Sbjct: 68 DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127
Query: 352 SVESRLSSDGVLSVIAPR 405
V++ + +GVL+V+ P+
Sbjct: 128 EVKATM-ENGVLTVVVPK 144
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 36.7 bits (81), Expect = 0.013
Identities = 25/78 (32%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
Frame = +1
Query: 196 DVQHFAPEEISVKTA-DGYIVVEG-KHEEKKDQHGYISR------QFTRRYALPEGCTAE 351
D+ EE+ V+ D + + G +H EK+D++ R QFTRR+ LPE +
Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121
Query: 352 SVESRLSSDGVLSVIAPR 405
V++ + +GVL+V P+
Sbjct: 122 QVKAAM-ENGVLTVTVPK 138
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 34.3 bits (75), Expect = 0.070
Identities = 23/78 (29%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
Frame = +1
Query: 196 DVQHFAPEEISVKTADGYIV-VEGKH----EEKKDQHGYISR---QFTRRYALPEGCTAE 351
D+ EE+ V+ D ++ + G+ EEK+D + R QF+R++ LPE +
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 352 SVESRLSSDGVLSVIAPR 405
V++ + +GVL+V P+
Sbjct: 124 QVKASM-ENGVLTVTVPK 140
>At1g78910.1 68414.m09199 pseudouridine synthase family protein low
similarity to SP|P23851 Ribosomal large subunit
pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
synthase) (Uracil hydrolyase) {Escherichia coli};
contains Pfam profile PF00849: RNA pseudouridylate
synthase
Length = 478
Score = 29.1 bits (62), Expect = 2.6
Identities = 23/59 (38%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = -2
Query: 361 TPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPR--CSRPPF*QRFLPGRNAGRPG 191
T I+ YS A I N A L P+ PR S PPF LPG+NAG G
Sbjct: 14 TVLIRRYSRVAPPPSSVIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKNAGADG 72
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 28.7 bits (61), Expect = 3.5
Identities = 17/54 (31%), Positives = 23/54 (42%)
Frame = +1
Query: 217 EEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSD 378
+EI D Y EG EE+ ++ R + L E C E+VES D
Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDD 101
>At5g04290.1 68418.m00422 KOW domain-containing transcription factor
family protein
Length = 1493
Score = 28.7 bits (61), Expect = 3.5
Identities = 16/44 (36%), Positives = 20/44 (45%)
Frame = +3
Query: 138 GSRPWLQHQK*QG*VPSKPGRPAFRPGRNLC*NGGRLHRG*RQT 269
GS+PW +H + G FR GRN GGR G R +
Sbjct: 1321 GSKPWNEHSGGGRGFGERRGGGGFRGGRNQSGRGGRSFDGGRSS 1364
>At3g49540.1 68416.m05414 expressed protein
Length = 166
Score = 28.3 bits (60), Expect = 4.6
Identities = 17/49 (34%), Positives = 28/49 (57%)
Frame = +1
Query: 334 EGCTAESVESRLSSDGVLSVIAPRKVPPAVEGERKIPIAQTGPVRKEVK 480
E E+ E+++ + ++V AP KV AVE E+K A+ PV+ E +
Sbjct: 100 ENAATENAEAKVEA---VAVAAPEKVEVAVEAEKK---AEAEPVKAEAE 142
>At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containing
protein low similarity to fertility restorer [Petunia x
hybrida] GI:22128587, post-transcriptional control of
chloroplast gene expression CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 991
Score = 27.5 bits (58), Expect = 8.0
Identities = 15/48 (31%), Positives = 20/48 (41%)
Frame = -2
Query: 382 HHLKTAGTPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPRCSRP 239
HHL + ++ P S P S + H SS PRCS+P
Sbjct: 26 HHLALSSKARVFPLSLPCNFSSRVSFKLQLHCAASSSSSVSPPRCSKP 73
>At1g34180.1 68414.m04239 no apical meristem (NAM) family protein
contains Pfam PF02365: No apical meristem (NAM) domain;
similar to NAM-like protein GI:8809651 from (Arabidopsis
thaliana)
Length = 564
Score = 27.5 bits (58), Expect = 8.0
Identities = 16/50 (32%), Positives = 24/50 (48%)
Frame = +1
Query: 256 VEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSDGVLSVIAPR 405
+E E D G RQF +P+G +AE ++S L ++ I PR
Sbjct: 229 IEKLLNEIPDAPGVNQRQFDEFVGVPQGNSAEVIQSTLLNNSSGEYIDPR 278
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,423,999
Number of Sequences: 28952
Number of extensions: 326214
Number of successful extensions: 916
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 879
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 916
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1324661040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -