Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0986
(544 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g36520.1 68417.m05185 trichohyalin-related low similarity to ... 36 0.013
At3g57780.1 68416.m06436 expressed protein 31 0.50
At5g60500.1 68418.m07587 undecaprenyl pyrophosphate synthetase f... 30 0.87
At4g08710.1 68417.m01439 hypothetical protein contains Pfam prof... 30 0.87
At3g28770.1 68416.m03591 expressed protein 30 0.87
At2g02720.1 68415.m00214 pectate lyase family protein similar to... 30 0.87
At5g11110.1 68418.m01297 sucrose-phosphate synthase, putative si... 29 1.5
At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam d... 29 2.0
At1g32750.1 68414.m04038 HAC13 protein (HAC13) identical to HAC1... 29 2.0
At5g06430.1 68418.m00720 thioredoxin-related contains weak simil... 29 2.7
At3g54290.1 68416.m06000 expressed protein contains Pfam PF03794... 29 2.7
At2g22795.1 68415.m02704 expressed protein 29 2.7
At2g17410.1 68415.m02009 ARID/BRIGHT DNA-binding domain-containi... 29 2.7
At1g09720.1 68414.m01091 kinase interacting family protein simil... 29 2.7
At1g11790.1 68414.m01353 prephenate dehydratase family protein s... 28 4.6
At5g45520.1 68418.m05591 hypothetical protein 27 6.1
At4g13780.1 68417.m02137 methionine--tRNA ligase, putative / met... 27 6.1
At1g23230.1 68414.m02906 expressed protein 27 6.1
At5g20540.1 68418.m02439 expressed protein 27 8.1
At5g15430.1 68418.m01806 calmodulin-binding protein-related has ... 27 8.1
At4g15820.1 68417.m02407 wound-responsive protein-related contai... 27 8.1
At1g34160.1 68414.m04237 pentatricopeptide (PPR) repeat-containi... 27 8.1
>At4g36520.1 68417.m05185 trichohyalin-related low similarity to
SP|Q07283 Trichohyalin {Homo sapiens}
Length = 1400
Score = 36.3 bits (80), Expect = 0.013
Identities = 25/114 (21%), Positives = 49/114 (42%), Gaps = 1/114 (0%)
Frame = +3
Query: 159 RDVDTNEKIVLPSAEDVATEKTQKSLFDGIEKFDSSQLKHTETQEKNPLPDKDAIEAEKE 338
R+ + NE+ + + E E+ K+ + EK Q+K + +E+N K+ +E +
Sbjct: 744 REKEENERRIKEAREKAELEQRLKATLEQEEK--ERQIKERQEREENERRAKEVLEQAEN 801
Query: 339 KNKFLNGIENFD-PTKLKHTETCEKNPLPTKDVXEQEKSAXXXXXXXXXANVSR 497
+ K +E + +LK T E+N ++ E E+ A + R
Sbjct: 802 ERKLKEALEQKENERRLKETREKEENKKKLREAIELEEKEKRLIEAFERAEIER 855
Score = 31.5 bits (68), Expect = 0.38
Identities = 21/95 (22%), Positives = 45/95 (47%), Gaps = 1/95 (1%)
Frame = +3
Query: 168 DTNEKIVLPSAEDVATEKTQKSLFDGIEKFDSSQLKHTETQEKNPLPDKDAIEAEKEKNK 347
+ NE+ E E+ K + +K + +LK T +E+N ++AIE E+++ +
Sbjct: 786 EENERRAKEVLEQAENERKLKEALE--QKENERRLKETREKEENKKKLREAIELEEKEKR 843
Query: 348 FLNGIENFD-PTKLKHTETCEKNPLPTKDVXEQEK 449
+ E + +LK E+ + ++ E+E+
Sbjct: 844 LIEAFERAEIERRLKEDLEQEEMRMRLQEAKERER 878
>At3g57780.1 68416.m06436 expressed protein
Length = 670
Score = 31.1 bits (67), Expect = 0.50
Identities = 21/63 (33%), Positives = 30/63 (47%), Gaps = 1/63 (1%)
Frame = +3
Query: 222 TQKSLFDGIEKFDSSQLKHTETQEK-NPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTE 398
TQ L D + + + TE +E N L D E EKE++K L+ I + + E
Sbjct: 58 TQSKLSDDDDHAVNDSSEKTEKEETINGLACDDEDEEEKEESKELDAIAHEKTDSVSSPE 117
Query: 399 TCE 407
TCE
Sbjct: 118 TCE 120
>At5g60500.1 68418.m07587 undecaprenyl pyrophosphate synthetase
family protein / UPP synthetase family protein contains
putative undecaprenyl diphosphate synthase domain
[PF01255]; similar to S. cerevisiae dehydrodolichyl
diphosphate synthetase (DEDOL-PP
synthase)(Rer2)[SP|P35196], a cis-prenyltransferase
Length = 271
Score = 30.3 bits (65), Expect = 0.87
Identities = 15/40 (37%), Positives = 23/40 (57%)
Frame = -3
Query: 221 LLSGNVFSRRKHNLFIGVDVTETAGVEAFELTLQVCGDLG 102
++ GN +KHNL IG+D AG + + LQ C ++G
Sbjct: 41 IMDGNRRFAKKHNL-IGLDAGHRAGFISVKYILQYCKEIG 79
>At4g08710.1 68417.m01439 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 715
Score = 30.3 bits (65), Expect = 0.87
Identities = 17/48 (35%), Positives = 25/48 (52%)
Frame = +3
Query: 201 EDVATEKTQKSLFDGIEKFDSSQLKHTETQEKNPLPDKDAIEAEKEKN 344
EDV EK D +++ D Q + ++EK +KD E EKEK+
Sbjct: 642 EDVVQEKDGDQEEDVVQEKDGDQEEDGVSKEKEQEKEKDPKEKEKEKD 689
>At3g28770.1 68416.m03591 expressed protein
Length = 2081
Score = 30.3 bits (65), Expect = 0.87
Identities = 21/81 (25%), Positives = 34/81 (41%)
Frame = +3
Query: 171 TNEKIVLPSAEDVATEKTQKSLFDGIEKFDSSQLKHTETQEKNPLPDKDAIEAEKEKNKF 350
TNE V E+V K + + EK +S K ET++ L + + KE++
Sbjct: 763 TNENRVRNKEENVQGNKKESEKVEKGEKKESKDAKSVETKDNKKLSSTENRDEAKERSGE 822
Query: 351 LNGIENFDPTKLKHTETCEKN 413
N + + + E EKN
Sbjct: 823 DNKEDKEESKDYQSVEAKEKN 843
Score = 29.1 bits (62), Expect = 2.0
Identities = 30/125 (24%), Positives = 49/125 (39%), Gaps = 1/125 (0%)
Frame = +3
Query: 75 SDTPSLKDLPKVATDLKSQLEGFNTSCLRDVDTNEKIVLPSAEDVATEKTQKSLFDGIEK 254
S T S D K D + + + + D K +++ KT ++ E+
Sbjct: 716 SQTDSKDD--KSVDDKQEEAQIYGGESKDDKSVEAKGKKKESKENKKTKTNENRVRNKEE 773
Query: 255 FDSSQLKHTETQEKNPLPD-KDAIEAEKEKNKFLNGIENFDPTKLKHTETCEKNPLPTKD 431
K +E EK + KDA E + NK L+ EN D K + E +++ +KD
Sbjct: 774 NVQGNKKESEKVEKGEKKESKDAKSVETKDNKKLSSTENRDEAKERSGEDNKEDKEESKD 833
Query: 432 VXEQE 446
E
Sbjct: 834 YQSVE 838
>At2g02720.1 68415.m00214 pectate lyase family protein similar to
pectate lyase P59 SP:P15722 from [Lycopersicon
esculentum]
Length = 455
Score = 30.3 bits (65), Expect = 0.87
Identities = 16/52 (30%), Positives = 22/52 (42%)
Frame = +3
Query: 273 KHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCEKNPLPTK 428
K +E P P+ + + EK+ FLNG K KNP+P K
Sbjct: 375 KQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPKNPIPRK 426
>At5g11110.1 68418.m01297 sucrose-phosphate synthase, putative
similar to sucrose-phosphate synthase isoform 1, Citrus
unshiu, PIR:S72648
Length = 894
Score = 29.5 bits (63), Expect = 1.5
Identities = 26/71 (36%), Positives = 36/71 (50%), Gaps = 6/71 (8%)
Frame = +3
Query: 69 SVSDTPS--LKDLPKVATDLKSQLEGFNTSCLRDVDTNEKIVLPSAEDVA-TEKTQKSLF 239
S SD+PS L+D+ ++ +LK L+G + VDTN +AE A EK +L
Sbjct: 532 SDSDSPSDSLRDINDISLNLKLSLDGEKSGSNNGVDTNLDAEDRAAERKAEVEKAVSTLA 591
Query: 240 D---GIEKFDS 263
EKFDS
Sbjct: 592 QKSKPTEKFDS 602
>At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam
domain PF04931: DNA polymerase V
Length = 1306
Score = 29.1 bits (62), Expect = 2.0
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +3
Query: 279 TETQEKNPLPDKDAIEAEKEKNKFLNGIENFD 374
TE E + LPD ++ +K K + +N + N+D
Sbjct: 12 TELVENDNLPDSSIVKKKKSKTEKMNTVANYD 43
>At1g32750.1 68414.m04038 HAC13 protein (HAC13) identical to HAC13
[Arabidopsis thaliana] gi|21105767|gb|AAM34782; contains
Pfam domains, PF00439: Bromodomain and PF00240:
Ubiquitin family
Length = 1919
Score = 29.1 bits (62), Expect = 2.0
Identities = 23/94 (24%), Positives = 42/94 (44%)
Frame = +3
Query: 165 VDTNEKIVLPSAEDVATEKTQKSLFDGIEKFDSSQLKHTETQEKNPLPDKDAIEAEKEKN 344
V + E +LP E +T SL FD E QE+ P + ++E EK +
Sbjct: 112 VVSEEDHLLPKKEYFSTAVALGSLKSRASVFDDEDYDEEEEQEEEQAPVEKSLETEKREP 171
Query: 345 KFLNGIENFDPTKLKHTETCEKNPLPTKDVXEQE 446
L + + + + + +++ + T+DV E+E
Sbjct: 172 VVLKEDKALEYEE-EASILDKEDHMDTEDVQEEE 204
>At5g06430.1 68418.m00720 thioredoxin-related contains weak
similarity to Swiss-Prot:Q9SEU7 thioredoxin M-type 3,
chloroplast precursor (TRX-M3) [Arabidopsis thaliana]
Length = 194
Score = 28.7 bits (61), Expect = 2.7
Identities = 21/73 (28%), Positives = 28/73 (38%), Gaps = 2/73 (2%)
Frame = -2
Query: 399 FPCASA*WD--RSSRCRSGICSFPSPLR*RLCPEAGSSPESRCASAGSNQTSRYRRIKTS 226
F C WD + + S +C F P R GS S S G N R ++ S
Sbjct: 17 FFCLKWPWDSNKQPKSSSSVCDFQGPWLFRSMQTIGSIALSSLTSFGQNPNFRPKKKPLS 76
Query: 225 GSSQWQRLQQTEA 187
S Q + Q+ A
Sbjct: 77 SSEQGEAEQRAFA 89
>At3g54290.1 68416.m06000 expressed protein contains Pfam PF03794:
Domain of Unknown function
Length = 350
Score = 28.7 bits (61), Expect = 2.7
Identities = 18/45 (40%), Positives = 25/45 (55%), Gaps = 3/45 (6%)
Frame = +3
Query: 228 KSLFDGIEKFDSSQLK---HTETQEKNPLPDKDAIEAEKEKNKFL 353
++LF +F S Q+ H E +EK+ LP +A E KEK K L
Sbjct: 252 EALFSLASRFKSLQMMCKTHFEEEEKDLLPMVEAAEMGKEKQKKL 296
>At2g22795.1 68415.m02704 expressed protein
Length = 734
Score = 28.7 bits (61), Expect = 2.7
Identities = 21/98 (21%), Positives = 44/98 (44%)
Frame = +3
Query: 159 RDVDTNEKIVLPSAEDVATEKTQKSLFDGIEKFDSSQLKHTETQEKNPLPDKDAIEAEKE 338
++ + EK+ S E ++T EK +SS L+ T+ +E +++ EK
Sbjct: 469 KETEAKEKVESSSQEKNEDKET--------EKIESSFLEETKEKEDETKEKEESSSQEKT 520
Query: 339 KNKFLNGIENFDPTKLKHTETCEKNPLPTKDVXEQEKS 452
+ K +N + + + T+ E + ++ QE+S
Sbjct: 521 EEKETETKDNEESSSQEETKDKENEKIEKEEASSQEES 558
Score = 28.7 bits (61), Expect = 2.7
Identities = 29/118 (24%), Positives = 58/118 (49%), Gaps = 5/118 (4%)
Frame = +3
Query: 114 TDLKSQLEGFNTSCLRDVDTNEKIVLPSAEDVATEKTQKSLFDGIEKFDSSQLKHTETQE 293
T+ K ++E + D +T EKI E E+T++ + EK +SS + TE +E
Sbjct: 471 TEAKEKVESSSQEKNEDKET-EKI-----ESSFLEETKEKEDETKEKEESSSQEKTEEKE 524
Query: 294 KNPLPDKDAIEAEKEKNKFLNGIE-----NFDPTKLKHTETCEKNPLPTKDVXEQEKS 452
++++ E+ K+K IE + + +K TET EK +++ +++++
Sbjct: 525 TETKDNEESSSQEETKDKENEKIEKEEASSQEESKENETETKEKEESSSQEETKEKEN 582
>At2g17410.1 68415.m02009 ARID/BRIGHT DNA-binding domain-containing
protein contains Pfam profile PF01388: ARID/BRIGHT DNA
binding domain
Length = 786
Score = 28.7 bits (61), Expect = 2.7
Identities = 18/58 (31%), Positives = 28/58 (48%), Gaps = 3/58 (5%)
Frame = +3
Query: 69 SVSDTPSLKDLPKVATDLKSQLEGF---NTSCLRDVDTNEKIVLPSAEDVATEKTQKS 233
S+ + + DLPK+ + SQ E + S +DT E ++ P+AED E S
Sbjct: 78 SLEEVTNADDLPKIDDEKNSQFETSPHPSPSPSVALDTEEGLINPTAEDTVEENIVSS 135
>At1g09720.1 68414.m01091 kinase interacting family protein similar
to kinase interacting protein 1 (GI:13936326) [Petunia
integrifolia]
Length = 928
Score = 28.7 bits (61), Expect = 2.7
Identities = 25/95 (26%), Positives = 40/95 (42%), Gaps = 3/95 (3%)
Frame = +3
Query: 63 ACSVSDTPSLKDLPKVATDLKSQLEGFNTSCLRDVDTNEKIVLPSAEDVATEKTQKSLFD 242
AC VSD+ D+ K T L+ +L R D N+ + E +T K
Sbjct: 384 ACLVSDS---MDMKKRITVLEDELRKVKNLFQRVEDQNKNLHKHLTEANSTAKDLSGKLQ 440
Query: 243 GI---EKFDSSQLKHTETQEKNPLPDKDAIEAEKE 338
+ E + L + QE++ + D D+I E+E
Sbjct: 441 EVKMDEDVEGDGLNPEDIQEEDTVEDSDSISNERE 475
>At1g11790.1 68414.m01353 prephenate dehydratase family protein
similar to gi|2392772 and is a member of the PF|00800
Prephenate dehydratase family. ESTs gb|T21562 and
gb|T21062 come from this gene
Length = 392
Score = 27.9 bits (59), Expect = 4.6
Identities = 19/60 (31%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
Frame = -2
Query: 351 GICSFPSPLR*RLCP-EAGSSPESRCASAGSNQTSRYRRIKTSGSSQWQRLQQTEAQSFH 175
G+ F + R R C E SS R +A + R +K S S + Q+T++ SFH
Sbjct: 23 GLAEFDADKRRRFCLWECSSSASQRAVTAIEGEIPFSRELKKS-SDELGLTQETQSLSFH 81
>At5g45520.1 68418.m05591 hypothetical protein
Length = 1167
Score = 27.5 bits (58), Expect = 6.1
Identities = 20/77 (25%), Positives = 38/77 (49%), Gaps = 1/77 (1%)
Frame = +3
Query: 222 TQKSLFDGIEKFDSSQLKHTET-QEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTE 398
T K L D +E+ D + +T +E+ ++ + + ++ +N N + K KH E
Sbjct: 575 TDKKLKDLMEREDDQVQNYGQTSKEEKGNVEETGKQEDGDQGDGINEEANLEDGK-KHDE 633
Query: 399 TCEKNPLPTKDVXEQEK 449
E+ L + +V E+EK
Sbjct: 634 GKEERSLKSDEVVEEEK 650
>At4g13780.1 68417.m02137 methionine--tRNA ligase, putative /
methionyl-tRNA synthetase, putative / MetRS, putative
similar to methionyl-tRNA synthetase [Oryza sativa]
GI:4091008; contains Pfam profiles PF00133: tRNA
synthetases class I (I, L, M and V), PF01588: Putative
tRNA binding domain
Length = 797
Score = 27.5 bits (58), Expect = 6.1
Identities = 32/102 (31%), Positives = 45/102 (44%), Gaps = 8/102 (7%)
Frame = +3
Query: 72 VSDTP-SLKDLP-KVATDLKSQLEGFNTSCLRDVDTNEKI----VLPSAEDVATEKTQKS 233
VSDT S DL K+ +L S L F L + + V+P A D + KS
Sbjct: 383 VSDTSFSWTDLQAKLNGELLSNLGNFVNRVLSFIAKPDNAGYGSVIPDAHDAESHSLTKS 442
Query: 234 LFDGIEKFDSSQLKHTETQEKNPLPD--KDAIEAEKEKNKFL 353
L + +EKF + ++ E EK L K A+ E N +L
Sbjct: 443 LAEKVEKFVA---EYVEAMEKVKLKQGLKTAMLISSEGNYYL 481
>At1g23230.1 68414.m02906 expressed protein
Length = 1615
Score = 27.5 bits (58), Expect = 6.1
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = -2
Query: 429 PLWGAGSFRTFPCASA*WDRSSRC-RSGICSFPSP 328
PL+G + P S W+R+ RC R I + PSP
Sbjct: 393 PLYGEDLAMSIPKGSLDWERAVRCIRHAIRTTPSP 427
>At5g20540.1 68418.m02439 expressed protein
Length = 384
Score = 27.1 bits (57), Expect = 8.1
Identities = 13/49 (26%), Positives = 24/49 (48%)
Frame = +3
Query: 72 VSDTPSLKDLPKVATDLKSQLEGFNTSCLRDVDTNEKIVLPSAEDVATE 218
++ TP + + T+ S +S RD D +E++ + +A DV E
Sbjct: 282 LNSTPKVSSISVAKTETSSIDASIRSSSSRDADRSEEMSVSNASDVDNE 330
>At5g15430.1 68418.m01806 calmodulin-binding protein-related has
weak similarity to calmodulin-binding proteins
Length = 478
Score = 27.1 bits (57), Expect = 8.1
Identities = 15/36 (41%), Positives = 18/36 (50%)
Frame = -2
Query: 372 RSSRCRSGICSFPSPLR*RLCPEAGSSPESRCASAG 265
R SR SG + SPLR + + SP RC S G
Sbjct: 70 RVSRSFSGAINLDSPLRKKALTKPLLSPSRRCDSVG 105
>At4g15820.1 68417.m02407 wound-responsive protein-related contains
weak similarity to KED [Nicotiana tabacum]
gi|8096269|dbj|BAA95789
Length = 460
Score = 27.1 bits (57), Expect = 8.1
Identities = 18/50 (36%), Positives = 27/50 (54%), Gaps = 3/50 (6%)
Frame = +3
Query: 45 IY*FRMACSV---SDTPSLKDLPKVATDLKSQLEGFNTSCLRDVDTNEKI 185
I+ F+ C+V D+ + P+V++D EG N L DV+ NEKI
Sbjct: 92 IFAFQTVCAVLFLGDSTKSEKTPEVSSDS----EGNNLVLLEDVEMNEKI 137
>At1g34160.1 68414.m04237 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 558
Score = 27.1 bits (57), Expect = 8.1
Identities = 21/65 (32%), Positives = 32/65 (49%), Gaps = 4/65 (6%)
Frame = -3
Query: 230 LLGLLSGNVFSRRKHNLFIGVDVTETAGVEAFELT----LQVCGDLGEVFQGGSVTHGAG 63
+ GL+SGN S L+ + ET G+ E+T L C LG+V +G ++ HG
Sbjct: 182 IAGLVSGNRASEAME-LYKRM---ETEGIRRSEVTVVAALGACSHLGDVKEGENIFHGYS 237
Query: 62 HSESI 48
+ I
Sbjct: 238 NDNVI 242
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,831,128
Number of Sequences: 28952
Number of extensions: 226985
Number of successful extensions: 910
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 858
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 910
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1013649368
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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