Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0983
(600 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g11780.1 68414.m01352 oxidoreductase, 2OG-Fe(II) oxygenase fa... 32 0.25
At2g42780.1 68415.m05297 expressed protein 32 0.33
At1g49270.1 68414.m05524 protein kinase family protein contains ... 29 1.8
At1g61370.1 68414.m06916 S-locus lectin protein kinase family pr... 29 3.1
At4g18670.1 68417.m02762 leucine-rich repeat family protein / ex... 28 4.1
At4g15740.1 68417.m02396 C2 domain-containing protein similar to... 28 5.4
At1g31250.1 68414.m03825 proline-rich family protein contains pr... 28 5.4
At1g06710.1 68414.m00713 pentatricopeptide (PPR) repeat-containi... 28 5.4
At5g57200.1 68418.m07145 epsin N-terminal homology (ENTH) domain... 27 7.2
At5g15980.1 68418.m01868 pentatricopeptide (PPR) repeat-containi... 27 7.2
At3g24550.1 68416.m03083 protein kinase family protein contains ... 27 7.2
At3g08760.1 68416.m01018 protein kinase family protein contains ... 27 7.2
At2g22530.1 68415.m02672 phosphatidylinositolglycan-related simi... 27 7.2
At1g75260.1 68414.m08743 isoflavone reductase family protein sim... 27 7.2
At1g61360.1 68414.m06915 S-locus lectin protein kinase family pr... 27 7.2
At1g10870.1 68414.m01249 ARF GTPase-activating domain-containing... 27 7.2
At1g77220.1 68414.m08994 expressed protein contains Pfam profile... 27 9.5
At1g52340.1 68414.m05908 short-chain dehydrogenase/reductase (SD... 27 9.5
At1g19940.1 68414.m02499 glycosyl hydrolase family 9 protein sim... 27 9.5
>At1g11780.1 68414.m01352 oxidoreductase, 2OG-Fe(II) oxygenase
family protein low similarity to alkB proteins from Homo
sapiens [SP|Q13686], Caulobacter crescentus
[GI:2055386]; contains Pfam profile PF03171:
oxidoreductase, 2OG-Fe(II) oxygenase family
Length = 345
Score = 32.3 bits (70), Expect = 0.25
Identities = 22/67 (32%), Positives = 35/67 (52%)
Frame = +2
Query: 365 ISMHQQALYNQQSTYTNIPTSPNISYSTKPNGPPIEDLFDTIKSNEPSVNKSLYNDHRFS 544
+S+ +Q + ++S T+ P PN + GP I+DLFD+ K N+ V L N+
Sbjct: 94 LSLKEQCKWIKESL-TSFPQPPNRTNHNAIYGP-IDDLFDSAKENKVLVQDDLTNNK--- 148
Query: 545 WLWK*YD 565
WK Y+
Sbjct: 149 --WKFYE 153
>At2g42780.1 68415.m05297 expressed protein
Length = 293
Score = 31.9 bits (69), Expect = 0.33
Identities = 14/53 (26%), Positives = 27/53 (50%)
Frame = +2
Query: 56 LLSKLKVGTLRLKEIKRANLVAKSYTYKRFTKMEPMTLGSPTHSPSGSPNVGY 214
++ K K+ L+ +E+K + ++ K F+ P G +SPS S ++ Y
Sbjct: 169 IMKKAKIDLLKSQEVKNLTAIKRNTIQKSFSISTPKKTGLSANSPSTSRSIPY 221
>At1g49270.1 68414.m05524 protein kinase family protein contains
Pfam domain PF00069: Protein kinase domain
Length = 699
Score = 29.5 bits (63), Expect = 1.8
Identities = 13/38 (34%), Positives = 17/38 (44%)
Frame = +2
Query: 353 YRQNISMHQQALYNQQSTYTNIPTSPNISYSTKPNGPP 466
Y QN + QQ YNQ N+P P + + PP
Sbjct: 229 YNQNAATQQQQHYNQNDHIVNLPPPPGSMGTNWVSSPP 266
>At1g61370.1 68414.m06916 S-locus lectin protein kinase family
protein contains Pfam domains, PF00954: S-locus
glycoprotein family, PF00069: Protein kinase domain, and
PF01453: Lectin (probable mannose binding)
Length = 814
Score = 28.7 bits (61), Expect = 3.1
Identities = 11/51 (21%), Positives = 25/51 (49%)
Frame = +2
Query: 71 KVGTLRLKEIKRANLVAKSYTYKRFTKMEPMTLGSPTHSPSGSPNVGYLPP 223
K+G + + ++ S + T+ P+++G SP+G+ +G+ P
Sbjct: 3 KIGIVFFASLLFLLIIFPSCAFAAITRASPLSIGQTLSSPNGTYELGFFSP 53
>At4g18670.1 68417.m02762 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 839
Score = 28.3 bits (60), Expect = 4.1
Identities = 17/46 (36%), Positives = 23/46 (50%), Gaps = 2/46 (4%)
Frame = +2
Query: 158 PMTLGSP--THSPSGSPNVGYLPPFLLGEINPPTTPRTNSLSPTKG 289
P+T+ SP T SP GSP + P P +P T+ +PT G
Sbjct: 433 PITVPSPPTTPSPGGSPPSPSIVPSPPSTTPSPGSPPTSPTTPTPG 478
>At4g15740.1 68417.m02396 C2 domain-containing protein similar to
cold-regulated gene SRC2 [Glycine max] GI:2055230;
contains Pfam profile PF00168: C2 domain
Length = 468
Score = 27.9 bits (59), Expect = 5.4
Identities = 13/45 (28%), Positives = 25/45 (55%)
Frame = +2
Query: 398 QSTYTNIPTSPNISYSTKPNGPPIEDLFDTIKSNEPSVNKSLYND 532
++T + T+ ++SY KP P+E+ + + PS+ K +Y D
Sbjct: 165 KATEKSTNTTVSLSYRFKP--VPVEEFYPPSPDSLPSIGKPVYRD 207
>At1g31250.1 68414.m03825 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965;
similar to prolin rich protein GB:S44189 GI:433706 from
[Zea mays]
Length = 165
Score = 27.9 bits (59), Expect = 5.4
Identities = 14/37 (37%), Positives = 21/37 (56%), Gaps = 2/37 (5%)
Frame = +2
Query: 176 PTHSPSGSPNVGYLPPFLLGEINPP-TTPRTN-SLSP 280
P +P+ SP+V PP+ + P TTP + SL+P
Sbjct: 109 PAGTPNASPSVKLTPPYASPSVRPTGTTPNASPSLTP 145
>At1g06710.1 68414.m00713 pentatricopeptide (PPR) repeat-containing
protein low similarity to fertility restorer [Petunia x
hybrida] GI:22128587; contains Pfam profile PF01535: PPR
repeat
Length = 946
Score = 27.9 bits (59), Expect = 5.4
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +2
Query: 26 TYTCLSTRI*LLSKLKVGTLRLKEIKRANLVAKSYTY 136
TY+ + + SK+++ L +E+KR LVA YTY
Sbjct: 444 TYSKVLNYLCNASKMELAFLLFEEMKRGGLVADVYTY 480
>At5g57200.1 68418.m07145 epsin N-terminal homology (ENTH)
domain-containing protein / clathrin assembly
protein-related low similarity to clathrin assembly
protein AP180 [Xenopus laevis] GI:6492344; contains Pfam
profile PF01417: ENTH domain
Length = 591
Score = 27.5 bits (58), Expect = 7.2
Identities = 19/59 (32%), Positives = 29/59 (49%), Gaps = 1/59 (1%)
Frame = +2
Query: 356 RQNISMHQQALYNQQ-STYTNIPTSPNISYSTKPNGPPIEDLFDTIKSNEPSVNKSLYN 529
+Q + M+ Q+ YN S Y + SPN S S+ PN P D F + + S + Y+
Sbjct: 529 QQMMMMNNQSPYNNNYSPYHHHQFSPNPSTSSSPN--PFGDPFLALPAPPSSTTQQQYS 585
>At5g15980.1 68418.m01868 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 668
Score = 27.5 bits (58), Expect = 7.2
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +2
Query: 455 NGPPIEDLFDTIKSNEPSVNKSL 523
N P + +LF+T+K NE +V K L
Sbjct: 642 NNPDVLELFNTMKPNESAVEKPL 664
>At3g24550.1 68416.m03083 protein kinase family protein contains
Pfam domain PF00069: Protein kinase domain
Length = 652
Score = 27.5 bits (58), Expect = 7.2
Identities = 16/46 (34%), Positives = 21/46 (45%), Gaps = 1/46 (2%)
Frame = +2
Query: 146 TKMEPMTLGSPTHSPSGSPN-VGYLPPFLLGEINPPTTPRTNSLSP 280
T P PT+S + +P PP +PP +P TNS SP
Sbjct: 9 TTPSPSPPSPPTNSTTTTPPPAASSPPPTTTPSSPPPSPSTNSTSP 54
>At3g08760.1 68416.m01018 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 557
Score = 27.5 bits (58), Expect = 7.2
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +2
Query: 242 NPPTTPRTNSLSPTKGRNLAF 304
+P PR S+SPT+GR L F
Sbjct: 501 SPSPPPRRPSVSPTRGRTLVF 521
>At2g22530.1 68415.m02672 phosphatidylinositolglycan-related similar
to Pig-o [Mus musculus] GI:8099973
Length = 897
Score = 27.5 bits (58), Expect = 7.2
Identities = 16/45 (35%), Positives = 24/45 (53%), Gaps = 2/45 (4%)
Frame = -1
Query: 498 LLFIVSNKSSMGGPFGLVE*D--MLGEVGILVYVLCWLYSACWCI 370
LL I S +S+ P+ + D L EVG +Y++ Y CWC+
Sbjct: 688 LLSISSIGASLVLPWSALNKDKSFLAEVGDCLYLIGSAYILCWCL 732
>At1g75260.1 68414.m08743 isoflavone reductase family protein
similar to SP|P52577 Isoflavone reductase homolog P3 (EC
1.3.1.-) {Arabidopsis thaliana}; contains Pfam profile
PF02716: Isoflavone reductase
Length = 593
Score = 27.5 bits (58), Expect = 7.2
Identities = 17/52 (32%), Positives = 22/52 (42%), Gaps = 1/52 (1%)
Frame = +2
Query: 143 FTKMEPMTLGSPTHSPSGSPNVGYLPPFLLGEINPPTTPRTN-SLSPTKGRN 295
F+ P + S T SPS P PPF I P+ T SP+ R+
Sbjct: 11 FSSNSPSRISSGTSSPSPPPTPPSRPPFRPAGIAQPSKSETKPKASPSLSRS 62
>At1g61360.1 68414.m06915 S-locus lectin protein kinase family
protein contains protein kinase domain, Pfam:PF00069;
contains S-locus glycoprotein family domain,
Pfam:PF00954
Length = 821
Score = 27.5 bits (58), Expect = 7.2
Identities = 11/30 (36%), Positives = 16/30 (53%)
Frame = +2
Query: 125 SYTYKRFTKMEPMTLGSPTHSPSGSPNVGY 214
SY Y T P+++G SP GS +G+
Sbjct: 16 SYGYAAITTSSPLSIGVTLSSPGGSYELGF 45
>At1g10870.1 68414.m01249 ARF GTPase-activating domain-containing
protein
Length = 775
Score = 27.5 bits (58), Expect = 7.2
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +3
Query: 402 AHILIFRLHPTYLTRLSRMVHPLKTYSTQ*KAMSRL 509
AH+ F+L L +L +H + TY+ Q K S++
Sbjct: 198 AHLRYFKLGYDLLNQLEPFIHQILTYAQQSKEQSKI 233
>At1g77220.1 68414.m08994 expressed protein contains Pfam profile
PF03619: Domain of unknown function
Length = 484
Score = 27.1 bits (57), Expect = 9.5
Identities = 12/43 (27%), Positives = 23/43 (53%)
Frame = -1
Query: 213 YPTLGLPEGECVGLPSVIGSIFVNLLYVYDLATKFARLISFNR 85
+P LG+ G+ + P + S+FV + + + F L S+N+
Sbjct: 28 HPNLGVDSGQYLTWPILSASVFVVIAILLPMYLIFEHLASYNQ 70
>At1g52340.1 68414.m05908 short-chain dehydrogenase/reductase (SDR)
family protein similar to stem secoisolariciresinol
dehydrogenase GI:13752458 from [Forsythia x intermedia]
Length = 285
Score = 27.1 bits (57), Expect = 9.5
Identities = 20/51 (39%), Positives = 23/51 (45%)
Frame = -1
Query: 258 GVVGGFISPSRKGGKYPTLGLPEGECVGLPSVIGSIFVNLLYVYDLATKFA 106
GVVGG S G K+ LGL L I VN + Y +ATK A
Sbjct: 163 GVVGGVGPHSYVGSKHAVLGLTRSVAAELGQ--HGIRVNCVSPYAVATKLA 211
>At1g19940.1 68414.m02499 glycosyl hydrolase family 9 protein
similar to endo-beta-1,4-D-glucanase GI:4165132 from
[Lycopersicon esculentum]
Length = 515
Score = 27.1 bits (57), Expect = 9.5
Identities = 11/35 (31%), Positives = 17/35 (48%)
Frame = +2
Query: 425 SPNISYSTKPNGPPIEDLFDTIKSNEPSVNKSLYN 529
+PN++Y GP + D F ++N S YN
Sbjct: 460 NPNVAYGALVGGPFLNDTFIDARNNSMQNEPSTYN 494
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,405,744
Number of Sequences: 28952
Number of extensions: 263680
Number of successful extensions: 753
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 717
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 752
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1187288784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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