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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0958
         (593 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_19071| Best HMM Match : Ribosomal_L4 (HMM E-Value=0)               122   3e-28
SB_31207| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   1.2  
SB_31407| Best HMM Match : TolA (HMM E-Value=2.5)                      30   1.6  
SB_25665| Best HMM Match : TolA (HMM E-Value=1.7)                      30   1.6  
SB_23808| Best HMM Match : zf-AN1 (HMM E-Value=5.5)                    28   6.5  
SB_31780| Best HMM Match : Pkinase (HMM E-Value=0)                     28   6.5  
SB_23350| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   6.5  
SB_22853| Best HMM Match : No HMM Matches (HMM E-Value=.)              27   8.6  

>SB_19071| Best HMM Match : Ribosomal_L4 (HMM E-Value=0)
          Length = 299

 Score =  122 bits (293), Expect = 3e-28
 Identities = 55/104 (52%), Positives = 72/104 (69%)
 Frame = +2

Query: 2   EKIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQR 181
           EKI E+PLV++D ++ + KT  AV  L+ + A+ D+ K   S+++RAGKGKMRNRR + R
Sbjct: 142 EKIAEVPLVISDAIESVTKTSAAVKLLKAVNAYEDVEKCIDSKKIRAGKGKMRNRRTVMR 201

Query: 182 KGPLIIFNKDQGLTRAFRNIPGVEXXXXXXXXXXXXAPGGHLGR 313
           KGPLII+N DQGL +AFRN+PGVE             PGGHLGR
Sbjct: 202 KGPLIIYNNDQGLRQAFRNLPGVELQHVDRLNLLKLCPGGHLGR 245



 Score = 34.7 bits (76), Expect = 0.057
 Identities = 17/31 (54%), Positives = 19/31 (61%)
 Frame = +3

Query: 501 RATRKLNPLTNNKAMLKLNPYAAVLKRKAIL 593
           RA  K NPL N   ML+LNPYA   KR  +L
Sbjct: 250 RAIHKKNPLKNLGTMLRLNPYAKSAKRAEML 280


>SB_31207| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 194

 Score = 30.3 bits (65), Expect = 1.2
 Identities = 17/50 (34%), Positives = 24/50 (48%)
 Frame = -2

Query: 172 TTTVAHFTLTSTKTLRLVHLKDIRPCLEAPQEDDSLFGLVDLLDFVGYNQ 23
           +T + HF     KT R +H+K        P E     GL+D+LD  G+ Q
Sbjct: 18  STVLHHFIDKHAKTPRFLHMKP-----NGPGEGGGSSGLLDMLDAAGFEQ 62


>SB_31407| Best HMM Match : TolA (HMM E-Value=2.5)
          Length = 315

 Score = 29.9 bits (64), Expect = 1.6
 Identities = 16/35 (45%), Positives = 21/35 (60%), Gaps = 1/35 (2%)
 Frame = -3

Query: 465 LISSDLRI-RVRSVLAIFGWGRLKFFFCFDGVFHD 364
           +IS+DL I R+  +L I  W R+  F CFDG   D
Sbjct: 281 IISADLEIQRITDLLTI--WARISDFDCFDGRISD 313


>SB_25665| Best HMM Match : TolA (HMM E-Value=1.7)
          Length = 697

 Score = 29.9 bits (64), Expect = 1.6
 Identities = 25/99 (25%), Positives = 47/99 (47%), Gaps = 2/99 (2%)
 Frame = -2

Query: 316 ETSKMASRSQLQEVQLVNI--QELHTGDVAEGASQTLILVEDYEGSLTLDTTTVAHFTLT 143
           +  +M  R QLQ++Q      Q+ H     +   Q  + ++  + S T DT+ V   +  
Sbjct: 290 QQQQMQQRQQLQQLQQQQQPQQQHHQQQQQQQQQQQQLFMQGIQLSST-DTSQVNMMSPL 348

Query: 142 STKTLRLVHLKDIRPCLEAPQEDDSLFGLVDLLDFVGYN 26
           S+ T ++  +    P L APQ++++L  + +LL     N
Sbjct: 349 SSPTQQVNPIPLNSPILPAPQQNNTLQNIQNLLTISSQN 387


>SB_23808| Best HMM Match : zf-AN1 (HMM E-Value=5.5)
          Length = 165

 Score = 27.9 bits (59), Expect = 6.5
 Identities = 21/65 (32%), Positives = 30/65 (46%), Gaps = 2/65 (3%)
 Frame = -2

Query: 247 TGDVAEGASQTLILVEDYE--GSLTLDTTTVAHFTLTSTKTLRLVHLKDIRPCLEAPQED 74
           T   AE A +    +E+    G +   +      T  STK LR + LKDI  C+E    +
Sbjct: 99  TSAAAEAALEPFQDIEETSALGQILPSSVDAVRLTKASTK-LRDIELKDIERCIEV---E 154

Query: 73  DSLFG 59
           D+ FG
Sbjct: 155 DTAFG 159


>SB_31780| Best HMM Match : Pkinase (HMM E-Value=0)
          Length = 964

 Score = 27.9 bits (59), Expect = 6.5
 Identities = 13/45 (28%), Positives = 26/45 (57%)
 Frame = -2

Query: 331 LSPDDETSKMASRSQLQEVQLVNIQELHTGDVAEGASQTLILVED 197
           L+ D+E   +   + L++V + +   + T  VA G S TL+L+++
Sbjct: 557 LALDNEDGPVEEVAVLRQVSVPDFNSVGTSSVAMGTSHTLVLLQN 601


>SB_23350| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 704

 Score = 27.9 bits (59), Expect = 6.5
 Identities = 12/31 (38%), Positives = 20/31 (64%)
 Frame = -2

Query: 220 QTLILVEDYEGSLTLDTTTVAHFTLTSTKTL 128
           Q LIL+  Y   + L+T+++  F+L S KT+
Sbjct: 235 QELILIPYYTEKVALETSSLIEFSLLSAKTI 265


>SB_22853| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 134

 Score = 27.5 bits (58), Expect = 8.6
 Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 1/30 (3%)
 Frame = -2

Query: 319 DETSKMASRSQLQEVQLVNIQE-LHTGDVA 233
           DETS  +SRS++Q   LV   E LH  D++
Sbjct: 39  DETSSPSSRSRIQASSLVKTNEFLHARDIS 68


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,948,867
Number of Sequences: 59808
Number of extensions: 400359
Number of successful extensions: 1410
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 1186
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1397
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1427401750
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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