Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0955
(673 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g21170.1 68415.m02511 triosephosphate isomerase, chloroplast,... 268 2e-72
At3g55440.1 68416.m06157 triosephosphate isomerase, cytosolic, p... 252 1e-67
At5g55820.1 68418.m06956 expressed protein 31 0.53
At1g25220.1 68414.m03130 anthranilate synthase beta subunit (ASB... 30 1.2
At1g31460.1 68414.m03852 expressed protein 30 1.6
At1g63250.1 68414.m07150 DEAD box RNA helicase, putative similar... 29 2.1
At1g61670.1 68414.m06956 expressed protein similar to membrane p... 29 2.8
At1g25540.1 68414.m03171 phytochrome and flowering time regulato... 29 3.7
At4g15380.1 68417.m02350 cytochrome P450 family protein similar ... 28 4.9
At3g07430.1 68416.m00886 YGGT family protein contains Pfam profi... 28 4.9
At5g57390.1 68418.m07170 ovule development protein, putative sim... 28 6.5
At5g51380.1 68418.m06370 F-box family protein contains Pfam PF00... 28 6.5
At2g12190.1 68415.m01316 cytochrome P450, putative 28 6.5
At1g64950.1 68414.m07362 cytochrome P450, putative similar to cy... 28 6.5
At1g64940.1 68414.m07361 cytochrome P450, putative similar to cy... 28 6.5
At1g24807.1 68414.m03108 anthranilate synthase beta subunit, put... 28 6.5
At5g57890.1 68418.m07242 anthranilate synthase beta subunit, put... 27 8.6
At4g08395.1 68417.m01387 hypothetical protein 27 8.6
At3g24710.1 68416.m03102 hypothetical protein 27 8.6
>At2g21170.1 68415.m02511 triosephosphate isomerase, chloroplast,
putative similar to Triosephosphate isomerase,
chloroplast precursor: SP|P48496 from Spinacia oleracea,
SP|P46225 from Secale cereale
Length = 315
Score = 268 bits (658), Expect = 2e-72
Identities = 125/224 (55%), Positives = 160/224 (71%)
Frame = +2
Query: 2 VGGNWKMNGDKNQINEIVNNLKKGPLDPNVEVIVGVPAIYLSYVKTIIPDNVEVAAQNCW 181
VGGNWK NG K+ I +++++L L+ +V+V+V P +Y+ VK+ + D ++++ QN W
Sbjct: 68 VGGNWKCNGTKDSIAKLISDLNSATLEADVDVVVSPPFVYIDQVKSSLTDRIDISGQNSW 127
Query: 182 KSPKGAFTGEISPAMIKDVGVNXVILGHSERRTIFGEKDELVAEKVAHALESGLKVIACI 361
GAFTGEIS +KD+G VILGHSERR + GEKDE + +K A+AL GL VIACI
Sbjct: 128 VGKGGAFTGEISVEQLKDLGCKWVILGHSERRHVIGEKDEFIGKKAAYALSEGLGVIACI 187
Query: 362 GETLEERESGKTEEVVFRQLKALVSAIGDKWENIVLAYEPVWAIGTGKTATPQQAQDVHH 541
GE LEERE+GKT +V F QLKA A+ W+NIV+AYEPVWAIGTGK A+PQQAQ+VH
Sbjct: 188 GEKLEEREAGKTFDVCFAQLKAFADAV-PSWDNIVVAYEPVWAIGTGKVASPQQAQEVHV 246
Query: 542 ALRNWLSANVSGSVSDAVRIQYGGSVTAANAKELASCKDIDGFL 673
A+R WL NVS V+ RI YGGSV N+ ELA +DIDGFL
Sbjct: 247 AVRGWLKKNVSEEVASKTRIIYGGSVNGGNSAELAKEEDIDGFL 290
>At3g55440.1 68416.m06157 triosephosphate isomerase, cytosolic,
putative strong similarity to triosephosphate isomerase,
cytosolic from Petunia hybrida [SP|P48495], from Coptis
japonica [SP|P21820]
Length = 254
Score = 252 bits (618), Expect = 1e-67
Identities = 122/226 (53%), Positives = 151/226 (66%), Gaps = 2/226 (0%)
Frame = +2
Query: 2 VGGNWKMNGDKNQINEIVNNLKKG--PLDPNVEVIVGVPAIYLSYVKTIIPDNVEVAAQN 175
VGGNWK NG ++ +IVN L + P VEV+V P ++L VK+ + + VAAQN
Sbjct: 7 VGGNWKCNGTAEEVKKIVNTLNEAQVPSQDVVEVVVSPPYVFLPLVKSTLRSDFFVAAQN 66
Query: 176 CWKSPKGAFTGEISPAMIKDVGVNXVILGHSERRTIFGEKDELVAEKVAHALESGLKVIA 355
CW GAFTGE+S M+ ++ + VILGHSERR I E E V +KVA+AL GLKVIA
Sbjct: 67 CWVKKGGAFTGEVSAEMLVNLDIPWVILGHSERRAILNESSEFVGDKVAYALAQGLKVIA 126
Query: 356 CIGETLEERESGKTEEVVFRQLKALVSAIGDKWENIVLAYEPVWAIGTGKTATPQQAQDV 535
C+GETLEERE+G T +VV Q KA+ + W N+V+AYEPVWAIGTGK A+P QAQ+V
Sbjct: 127 CVGETLEEREAGSTMDVVAAQTKAIADRV-TNWSNVVIAYEPVWAIGTGKVASPAQAQEV 185
Query: 536 HHALRNWLSANVSGSVSDAVRIQYGGSVTAANAKELASCKDIDGFL 673
H LR WL+ NVS V+ RI YGGSV N KEL D+DGFL
Sbjct: 186 HDELRKWLAKNVSADVAATTRIIYGGSVNGGNCKELGGQADVDGFL 231
>At5g55820.1 68418.m06956 expressed protein
Length = 1826
Score = 31.5 bits (68), Expect = 0.53
Identities = 23/78 (29%), Positives = 39/78 (50%)
Frame = -1
Query: 409 NNFLSLARFSLFESLTNTGNDFQARFKGMSNLFSNKFIFFTKNCSSFRMTKYNPIYSNVF 230
+ +S AR SL E + ND++A G ++ ++K + + S R T P++ + F
Sbjct: 737 HGIISRARSSLIEE--ESANDYKALSDGSNHKSADKQLEVREGNSLLR-TPDRPVFVDNF 793
Query: 229 DHSG*NFTSKSSFWRLPT 176
D N KSS ++PT
Sbjct: 794 DEVPENSREKSSMEKVPT 811
>At1g25220.1 68414.m03130 anthranilate synthase beta subunit (ASB1)
identical to anthranilate synthase beta subunit
GI:403434 from [Arabidopsis thaliana]
Length = 276
Score = 30.3 bits (65), Expect = 1.2
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = -1
Query: 172 LGCNFDIIRNDSLHIRQVNSRNTNNYL 92
LGC+F++ RND L + ++ +N L
Sbjct: 95 LGCHFEVYRNDELTVEELKKKNPRGVL 121
>At1g31460.1 68414.m03852 expressed protein
Length = 301
Score = 29.9 bits (64), Expect = 1.6
Identities = 14/38 (36%), Positives = 19/38 (50%)
Frame = -2
Query: 450 LSPMADTKAFNCLKTTSSVLPDSLSSRVSPIQAMTFKP 337
LS + A NC T S LPD L SR ++++ P
Sbjct: 25 LSDLEQVAAINCSGFTDSTLPDDLESRFRRLKSLPAAP 62
>At1g63250.1 68414.m07150 DEAD box RNA helicase, putative similar to
RNA helicase (RH25) [Arabidopsis thaliana] GI:3776023;
contains Pfam profiles PF00270: DEAD/DEAH box helicase,
PF00271: Helicase conserved C-terminal domain
Length = 798
Score = 29.5 bits (63), Expect = 2.1
Identities = 27/86 (31%), Positives = 41/86 (47%)
Frame = -1
Query: 556 PVTKSMVDILSLLGSSSLACTNSPYRFIGKHNIFPFVTNGRY*SF*LSKNNFLSLARFSL 377
PV KS D S GS S+ T++ + + + G+ F S+ F S++RF
Sbjct: 125 PVKKS--DQGSRSGSDSIDSTSNSPIDVRRLVSATCDSMGKNRVFGSSRRGFSSMSRFKR 182
Query: 376 FESLTNTGNDFQARFKGMSNLFSNKF 299
ES + G+DF A+ + FS KF
Sbjct: 183 NESSCDEGDDFDAKKLDTLSPFSPKF 208
>At1g61670.1 68414.m06956 expressed protein similar to membrane
protein PTM1 precursor isolog GI:1931644 from
[Arabidopsis thaliana]
Length = 513
Score = 29.1 bits (62), Expect = 2.8
Identities = 12/46 (26%), Positives = 21/46 (45%), Gaps = 2/46 (4%)
Frame = +2
Query: 95 VIVGVPAIYLSYVKTIIP--DNVEVAAQNCWKSPKGAFTGEISPAM 226
V++ +Y Y P D + + WK+P G G+++P M
Sbjct: 175 VVINKTGMYYLYFMICDPELDGTRIKGRTVWKNPDGYLPGKVAPLM 220
>At1g25540.1 68414.m03171 phytochrome and flowering time regulatory
protein (PFT1) PMID: 12815435
Length = 836
Score = 28.7 bits (61), Expect = 3.7
Identities = 19/60 (31%), Positives = 29/60 (48%)
Frame = -3
Query: 482 QVHRQAQYFPICHQWPILKLLIV*KQLPQSCQILSLRESHQYRQ*LSSQIQGHEQPFQQQ 303
Q+H+Q Q Q + + +Q+PQ Q + Q +Q SQ+Q H+Q QQQ
Sbjct: 688 QLHQQQQQQQQIQQQQQQQQHLQQQQMPQLQQQQQQHQQQQQQQHQLSQLQHHQQQQQQQ 747
>At4g15380.1 68417.m02350 cytochrome P450 family protein similar to
CYTOCHROME P450 93A3 (P450 CP5) (SP:O81973) [Glycine
max]
Length = 517
Score = 28.3 bits (60), Expect = 4.9
Identities = 13/30 (43%), Positives = 18/30 (60%), Gaps = 1/30 (3%)
Frame = -2
Query: 258 SITXFTPTS-LIIAGEISPVKAPFGDFQQF 172
S FTPT + AG S + AP+GD+ +F
Sbjct: 108 SYRGFTPTDDSLFAGSFSFISAPYGDYWKF 137
>At3g07430.1 68416.m00886 YGGT family protein contains Pfam profile
PF02325: YGGT family (unknown function)
Length = 232
Score = 28.3 bits (60), Expect = 4.9
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = -2
Query: 261 PSITXFTPTSLIIAGEISPVKAPFGDFQQFWAATSTLSG 145
PS++ P S+ I+ SP+ P ++ A +STL+G
Sbjct: 44 PSLSIQNPKSIRISASASPITTPILQTEKSTARSSTLTG 82
>At5g57390.1 68418.m07170 ovule development protein, putative
similar to ovule development protein AINTEGUMENTA
(GI:1209099)[Arabidopsis thaliana]
Length = 555
Score = 27.9 bits (59), Expect = 6.5
Identities = 15/49 (30%), Positives = 21/49 (42%)
Frame = +2
Query: 446 DKWENIVLAYEPVWAIGTGKTATPQQAQDVHHALRNWLSANVSGSVSDA 592
D + N A PV AI T T + + NW++ N+S S A
Sbjct: 492 DGYNNHSAASNPVSAIPLSSTTTMSNGNEGYGGNINWINNNISSSYQTA 540
>At5g51380.1 68418.m06370 F-box family protein contains Pfam
PF00646: F-box domain; similar to F-box protein FBL2
(GI:6063090) [Homo sapiens]
Length = 479
Score = 27.9 bits (59), Expect = 6.5
Identities = 13/52 (25%), Positives = 26/52 (50%)
Frame = -2
Query: 483 TGS*ASTIFSHLSPMADTKAFNCLKTTSSVLPDSLSSRVSPIQAMTFKPDSR 328
T S ++ H + + +C S + +LSS S ++ +T++PD+R
Sbjct: 405 TTSGLESVILHWEELESMRVVSCKNIKDSEISAALSSLFSLLKELTWRPDTR 456
>At2g12190.1 68415.m01316 cytochrome P450, putative
Length = 512
Score = 27.9 bits (59), Expect = 6.5
Identities = 15/45 (33%), Positives = 28/45 (62%)
Frame = +2
Query: 287 GEKDELVAEKVAHALESGLKVIACIGETLEERESGKTEEVVFRQL 421
GE+ +V + + +A+ + L V+ C G+ L+E++ + E V RQL
Sbjct: 168 GEEPIVVVDHLHYAMFA-LLVLMCFGDKLDEKQIKQVEYVQRRQL 211
>At1g64950.1 68414.m07362 cytochrome P450, putative similar to
cytochrome P450 89A2 (CYPLXXXIX) (SP:Q42602)
[Arabidopsis thaliana];similar to cytochrome P450
(GI:438242) [Solanum melongena]
Length = 510
Score = 27.9 bits (59), Expect = 6.5
Identities = 15/45 (33%), Positives = 28/45 (62%)
Frame = +2
Query: 287 GEKDELVAEKVAHALESGLKVIACIGETLEERESGKTEEVVFRQL 421
GE+ +V + + +A+ + L V+ C G+ L+E++ + E V RQL
Sbjct: 168 GEEPIVVVDHLHYAMFA-LLVLMCFGDKLDEKQIKQVEYVQRRQL 211
>At1g64940.1 68414.m07361 cytochrome P450, putative similar to
cytochrome p450 GI:438242 from [Solanum melongena]
Length = 511
Score = 27.9 bits (59), Expect = 6.5
Identities = 15/45 (33%), Positives = 28/45 (62%)
Frame = +2
Query: 287 GEKDELVAEKVAHALESGLKVIACIGETLEERESGKTEEVVFRQL 421
GE+ +V + + +A+ + L V+ C G+ L+E++ + E V RQL
Sbjct: 169 GEEPIVVVDHLHYAMFA-LLVLMCFGDKLDEKQIKQVEYVQRRQL 212
>At1g24807.1 68414.m03108 anthranilate synthase beta subunit,
putative similar to anthranilate synthase beta chain
GI:403434; similar to ESTs dbj|AV540153.1,
dbj|AV557490.1, gb|AI997696.1, gb|AW004516.1,
dbj|AV521371.1
Length = 235
Score = 27.9 bits (59), Expect = 6.5
Identities = 12/41 (29%), Positives = 21/41 (51%)
Frame = -1
Query: 214 NFTSKSSFWRLPTVLGCNFDIIRNDSLHIRQVNSRNTNNYL 92
NF + F + L C+F++ RND L + ++ +N L
Sbjct: 40 NFENCYLFLQYMGELQCHFEVYRNDELTVEELKRKNPRGVL 80
>At5g57890.1 68418.m07242 anthranilate synthase beta subunit,
putative strong similarity to anthranilate synthase beta
chain GI:403434 [Arabidopsis thaliana]
Length = 273
Score = 27.5 bits (58), Expect = 8.6
Identities = 9/27 (33%), Positives = 16/27 (59%)
Frame = -1
Query: 172 LGCNFDIIRNDSLHIRQVNSRNTNNYL 92
LGC+F++ RND L + ++ + L
Sbjct: 92 LGCHFEVYRNDELTVEELKRKKPRGLL 118
>At4g08395.1 68417.m01387 hypothetical protein
Length = 232
Score = 27.5 bits (58), Expect = 8.6
Identities = 17/46 (36%), Positives = 24/46 (52%)
Frame = +3
Query: 528 KMSTMLFVTGCRPM*VGVYLTLYVFSTVARSQLLMLRSLHPAKTLM 665
K S+ML V CR M VG L++ S+ L + +LH K +M
Sbjct: 185 KESSMLVVEKCRRMVVGKLCKLWITSSEFLVYDLTVHALHYLKLIM 230
>At3g24710.1 68416.m03102 hypothetical protein
Length = 126
Score = 27.5 bits (58), Expect = 8.6
Identities = 17/48 (35%), Positives = 26/48 (54%)
Frame = +2
Query: 296 DELVAEKVAHALESGLKVIACIGETLEERESGKTEEVVFRQLKALVSA 439
D V + E V++ +GE + E E+G ++EV R+LKAL A
Sbjct: 11 DGAVVYVAGSSTEMPSDVMSALGEIVSE-ETGGSKEVASRRLKALEKA 57
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,465,475
Number of Sequences: 28952
Number of extensions: 315571
Number of successful extensions: 910
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 883
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 907
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1422784080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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