Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0949
(759 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 46 4e-05
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 45 5e-05
At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chlo... 45 6e-05
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 44 1e-04
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 44 1e-04
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 44 1e-04
At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein (HS... 43 3e-04
At1g52560.1 68414.m05933 26.5 kDa class I small heat shock prote... 38 0.007
At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 33 0.27
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 32 0.48
At3g15250.1 68416.m01926 expressed protein ; expression supporte... 29 3.4
At3g12020.1 68416.m01490 kinesin motor protein-related similar t... 29 3.4
At4g34020.1 68417.m04827 DJ-1 family protein similar to CAP1 [Ra... 29 4.4
At5g55300.1 68418.m06891 DNA topoisomerase I identical to Swiss-... 28 7.7
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 45.6 bits (103), Expect = 4e-05
Identities = 42/163 (25%), Positives = 75/163 (46%), Gaps = 8/163 (4%)
Frame = +3
Query: 72 MSLLPFILGYQPNRLRDQDFGLALTPDDLLTAVITPTMSRDYYRPWKQMAMANRDVGSTI 251
MSL+P I G + + D +L D +TP+ + A N V
Sbjct: 1 MSLIPSIFGGRRTNVFDP---FSLDVFDPFEGFLTPSGLANA-PAMDVAAFTNAKVDWRE 56
Query: 252 TSNKDKFQVNLDVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTR 407
T F+ D+ EE+ V+ DG ++ + G+ +EE+ D+ + R +FTR
Sbjct: 57 TPEAHVFKA--DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTR 114
Query: 408 RYALPENCNPDTVESRLSSDGVLTVIAPRTPAATKNERAVPIT 536
R+ LPEN + +++ + +GVL+V P+ P +++ I+
Sbjct: 115 RFRLPENAKMEEIKASM-ENGVLSVTVPKVPEKKPEVKSIDIS 156
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 45.2 bits (102), Expect = 5e-05
Identities = 31/95 (32%), Positives = 49/95 (51%), Gaps = 8/95 (8%)
Frame = +3
Query: 285 DVQHFSPEEISVKTADGYVI-VEGKH----EERQDEHGYISR---QFTRRYALPENCNPD 440
D+ EE+ V+ D V+ + G+ EE+QD + R QF+R++ LPEN D
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 441 TVESRLSSDGVLTVIAPRTPAATKNERAVPITQTG 545
V++ + +GVLTV P+ A K + I +G
Sbjct: 124 QVKASM-ENGVLTVTVPKVEEAKKKAQVKSIDISG 157
>At4g27670.1 68417.m03979 25.3 kDa small heat shock protein,
chloroplast precursor (HSP25.3-P) identical to small
heat shock protein, chloroplast precursor SP:P31170 from
[Arabidopsis thaliana]; identified in Scharf, K-D., et
al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 227
Score = 44.8 bits (101), Expect = 6e-05
Identities = 20/91 (21%), Positives = 45/91 (49%), Gaps = 3/91 (3%)
Frame = +3
Query: 249 ITSNKDKFQVNLDVQHFSPEEISVKTADGYVIVEGKHEERQDEHGYISRQ---FTRRYAL 419
I + + ++ D+ S E++ + D ++++G+ ++ + + R + R L
Sbjct: 131 IKEEEHEIKMRFDMPGLSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQL 190
Query: 420 PENCNPDTVESRLSSDGVLTVIAPRTPAATK 512
P+NC D +++ L +GVL + P+T K
Sbjct: 191 PDNCEKDKIKAEL-KNGVLFITIPKTKVERK 220
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 44.0 bits (99), Expect = 1e-04
Identities = 27/92 (29%), Positives = 50/92 (54%), Gaps = 8/92 (8%)
Frame = +3
Query: 285 DVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPD 440
D+ EE+ V+ D V+ + G+ +EE+ D+ + R +F RR+ LPEN +
Sbjct: 68 DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127
Query: 441 TVESRLSSDGVLTVIAPRTPAATKNERAVPIT 536
V++ + +GVLTV+ P+ P +++ I+
Sbjct: 128 EVKATM-ENGVLTVVVPKAPEKKPQVKSIDIS 158
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 43.6 bits (98), Expect = 1e-04
Identities = 44/164 (26%), Positives = 75/164 (45%), Gaps = 9/164 (5%)
Frame = +3
Query: 72 MSLLPFILGYQPNRLRDQDFGLALTPDDLLTAVITPTMSRDYYRPWKQMA-MANRDVGST 248
MSL+P G + + D +L D +TP ++ P K +A N V
Sbjct: 1 MSLVPSFFGGRRTNVFDP---FSLDVWDPFEGFLTPGLTN---APAKDVAAFTNAKVDWR 54
Query: 249 ITSNKDKFQVNLDVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFT 404
T F+ DV EE+ V+ DG ++ + G+ +EE+ D + R +F
Sbjct: 55 ETPEAHVFKA--DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFM 112
Query: 405 RRYALPENCNPDTVESRLSSDGVLTVIAPRTPAATKNERAVPIT 536
RR+ LPEN + V++ + +GVL+V P+ + ++V I+
Sbjct: 113 RRFRLPENAKVEEVKASM-ENGVLSVTVPKVQESKPEVKSVDIS 155
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 43.6 bits (98), Expect = 1e-04
Identities = 28/92 (30%), Positives = 50/92 (54%), Gaps = 8/92 (8%)
Frame = +3
Query: 285 DVQHFSPEEISVKTADGYVI-VEG-KHEERQDEHGYISR------QFTRRYALPENCNPD 440
D+ EE+ V+ + V+ + G +H E++D++ R QFTRR+ LPEN D
Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121
Query: 441 TVESRLSSDGVLTVIAPRTPAATKNERAVPIT 536
V++ + +GVLTV P+ + +++ I+
Sbjct: 122 QVKAAM-ENGVLTVTVPKAETKKADVKSIQIS 152
>At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein
(HSP17.6A-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]
Length = 155
Score = 42.7 bits (96), Expect = 3e-04
Identities = 35/117 (29%), Positives = 56/117 (47%), Gaps = 8/117 (6%)
Frame = +3
Query: 219 AMANRDVGSTITSNKDKFQVNLDVQHFSPEEISVKTADGYVI-VEGKH----EERQDEHG 383
A+AN V T+ F+ D+ EE+ V+ D V+ + G+ EE+QD
Sbjct: 42 AIANARVDWKETAEAHVFKA--DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWH 99
Query: 384 YISRQ---FTRRYALPENCNPDTVESRLSSDGVLTVIAPRTPAATKNERAVPITQTG 545
+ R F+R++ LPEN D V++ + +GVLTV P+ K + I +G
Sbjct: 100 RVERSSGGFSRKFRLPENVKMDQVKASM-ENGVLTVTVPKVETNKKKAQVKSIDISG 155
>At1g52560.1 68414.m05933 26.5 kDa class I small heat shock
protein-like (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family; identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 232
Score = 37.9 bits (84), Expect = 0.007
Identities = 21/104 (20%), Positives = 51/104 (49%), Gaps = 9/104 (8%)
Frame = +3
Query: 249 ITSNKDKFQVNLDVQHFSPEEISVKTADGYVIVEGKHEERQD-----EHGYISRQ----F 401
+ D +++ +V + E++ + DG + ++G H+ ++ E Y S + +
Sbjct: 129 VKEQDDCYKLRYEVPGLTKEDVKITVNDGILTIKGDHKAEEEKGSPEEDEYWSSKSYGYY 188
Query: 402 TRRYALPENCNPDTVESRLSSDGVLTVIAPRTPAATKNERAVPI 533
+LP++ + +++ L + GVL ++ PRT KN + + +
Sbjct: 189 NTSLSLPDDAKVEDIKAELKN-GVLNLVIPRTEKPKKNVQEISV 231
>At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock
protein (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family: identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 134
Score = 32.7 bits (71), Expect = 0.27
Identities = 20/81 (24%), Positives = 36/81 (44%)
Frame = +3
Query: 252 TSNKDKFQVNLDVQHFSPEEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENC 431
+ + D ++D+ EEI V+ D ++ + F R++ LPE+
Sbjct: 31 SQSPDSHTFSVDLPGLRKEEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESI 90
Query: 432 NPDTVESRLSSDGVLTVIAPR 494
+ + + DGVLTVI P+
Sbjct: 91 DMIGISAGYE-DGVLTVIVPK 110
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 31.9 bits (69), Expect = 0.48
Identities = 28/103 (27%), Positives = 39/103 (37%), Gaps = 4/103 (3%)
Frame = +3
Query: 306 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTVI 485
+EI D Y EG EE ++ R + L E+C + VES D
Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDDDDDDDD 107
Query: 486 APRTPAATKNERAVPIT----QTGPVRKEIKEPTAEVESNETK 602
R KN +PI+ ++ R EP E E N T+
Sbjct: 108 DDRHSRMLKNVTELPISAFQGESKNKRVVFTEPYPESEFNPTR 150
>At3g15250.1 68416.m01926 expressed protein ; expression supported
by MPSS
Length = 217
Score = 29.1 bits (62), Expect = 3.4
Identities = 16/53 (30%), Positives = 25/53 (47%)
Frame = +3
Query: 429 CNPDTVESRLSSDGVLTVIAPRTPAATKNERAVPITQTGPVRKEIKEPTAEVE 587
CN S S+ T +PRT +T N+ PI Q P+ ++K P + +
Sbjct: 75 CNSTNSSSSFGSNNSSTSQSPRTSYSTSNKNNSPINQE-PLDLKLKPPISRCD 126
>At3g12020.1 68416.m01490 kinesin motor protein-related similar to
putative kinesin heavy chain GB:AAD23684 GI:4567271 from
[Arabidopsis thaliana]
Length = 1030
Score = 29.1 bits (62), Expect = 3.4
Identities = 24/95 (25%), Positives = 43/95 (45%), Gaps = 6/95 (6%)
Frame = +3
Query: 330 DGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTVI----APRT 497
D YV VEG HE R ++ Y + TR++ L P + S+ +V+ P T
Sbjct: 524 DLYVSVEGNHEIR--DNAYREEKKTRKHGLLNWLKPKKRDHSSSASDQSSVVKSNSTPST 581
Query: 498 P--AATKNERAVPITQTGPVRKEIKEPTAEVESNE 596
P + +++ P+ +++ EP + E+ E
Sbjct: 582 PQGGGSHLHTESRLSEGSPLMEQLSEPREDREALE 616
>At4g34020.1 68417.m04827 DJ-1 family protein similar to CAP1
[Rattus norvegicus] GI:3250916, ThiJ protein,
Escherichia coli, PIR:H64771; contains Pfam profile
PF01965 ThiJ/PfpI family
Length = 472
Score = 28.7 bits (61), Expect = 4.4
Identities = 16/43 (37%), Positives = 20/43 (46%)
Frame = -2
Query: 455 PRFDSVRVAVFRQSVTPRELTRDVAMLVLPLLVLTFDDDVAVG 327
P F SV V T R+ T + + P +V T D DV VG
Sbjct: 36 PSFSSVSVVSSSLGTTRRDRTLKLRSSMSPGMVTTLDSDVGVG 78
>At5g55300.1 68418.m06891 DNA topoisomerase I identical to
Swiss-Prot:P30181 DNA topoisomerase I [Arabidopsis
thaliana]
Length = 916
Score = 27.9 bits (59), Expect = 7.7
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = +3
Query: 507 TKNERAVPITQTGPVRKEIKEPTAEVESNET 599
++ +AVP T+ P+R + P SN+T
Sbjct: 43 SQRSKAVPTTKVSPMRSPVTSPNGTTPSNKT 73
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,592,565
Number of Sequences: 28952
Number of extensions: 294263
Number of successful extensions: 963
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 935
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 962
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1692519896
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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