Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0947
(709 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g17510.1 68417.m02620 ubiquitin carboxyl-terminal hydrolase, ... 140 8e-34
At1g65650.1 68414.m07448 ubiquitin carboxyl-terminal hydrolase f... 65 4e-11
At5g16310.1 68418.m01907 ubiquitin carboxyl-terminal hydrolase f... 62 3e-10
At1g65010.1 68414.m07368 expressed protein similar to endosome-a... 41 0.001
At5g10500.1 68418.m01216 kinase interacting family protein simil... 31 0.99
At3g02930.1 68416.m00288 expressed protein ; expression support... 29 4.0
At2g19910.1 68415.m02327 RNA-dependent RNA polymerase family pro... 28 7.0
At1g53020.1 68414.m06002 ubiquitin-conjugating enzyme family pro... 28 7.0
At3g27590.1 68416.m03447 hypothetical protein 27 9.2
>At4g17510.1 68417.m02620 ubiquitin carboxyl-terminal hydrolase,
putative / ubiquitin thiolesterase, putative similar to
SP|Q9JKB1 Ubiquitin carboxyl-terminal hydrolase isozyme
L3 (EC 3.4.19.12) (UCH- L3) (Ubiquitin thiolesterase L3)
{Mus musculus}; contains Pfam profile PF01088: Ubiquitin
carboxyl-terminal hydrolase, family 1
Length = 234
Score = 140 bits (339), Expect = 8e-34
Identities = 77/183 (42%), Positives = 110/183 (60%), Gaps = 2/183 (1%)
Frame = +2
Query: 164 VPLESNPDVLNKFLQKLGV-PNKWNIVDVMGLDPETLSWVPRPVLSVMLLFPISDAYENH 340
+PLESNPDV+N++L LG+ P++ DV GLD E L VP+PVL+V+ L+PI+ E
Sbjct: 14 LPLESNPDVMNQYLWGLGLAPDEAECNDVYGLDDELLEMVPKPVLAVLFLYPITKKSEEE 73
Query: 341 KKTEENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANNTDIIELSDG-HMQKFLNE 517
+ ++ EI K S +++MKQ + NACGTI L+H++ N T I+LSDG + +F
Sbjct: 74 RIEQDKEIKEKVH--SDKVYFMKQTVGNACGTIGLLHAIGNITSEIKLSDGSFLDRFFKS 131
Query: 518 AKGLDATARGKLLEKSEGIINAHKELAQEGQTNTPSAEDPVNHHFISFVQKDGALYELNG 697
+ R K LE I +AH G +TP++ED + HFI +G LYEL+G
Sbjct: 132 TANMTPMERAKFLENDSQIEDAHSVAVIAG--DTPASED-ADTHFICLACVEGELYELDG 188
Query: 698 RKA 706
RKA
Sbjct: 189 RKA 191
>At1g65650.1 68414.m07448 ubiquitin carboxyl-terminal hydrolase
family 1 protein similar to 26S proteasome regulatory
complex subunit p37A [Drosophila melanogaster]
GI:6434962; contains Pfam profile PF01088: Ubiquitin
carboxyl-terminal hydrolase, family 1
Length = 330
Score = 65.3 bits (152), Expect = 4e-11
Identities = 49/183 (26%), Positives = 91/183 (49%), Gaps = 5/183 (2%)
Frame = +2
Query: 170 LESNPDVLNKFLQKLGVPNKWNIVDVMGLDPETLSWVPRPVLSVMLLFPISDAYENHKKT 349
+ES+P V + +Q++ V + ++ LD ++L+ + RPV ++ LF ++ + T
Sbjct: 6 IESDPGVFTELIQQMQVKGV-QVEELYSLDSDSLNNL-RPVYGLIFLFKWQAGEKDERPT 63
Query: 350 EENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANNTDIIELSDGHMQKFLNE-AKG 526
++++ N+F+ Q I+NAC T A++ + N+ E+ G L E K
Sbjct: 64 IQDQV--------SNLFFANQVINNACATQAILAILLNSP---EVDIGPELSALKEFTKN 112
Query: 527 LDATARGKLLEKSEGIINAHKELAQEG----QTNTPSAEDPVNHHFISFVQKDGALYELN 694
+ +G + S+ I AH A+ + + +D +HFIS++ DG LYEL+
Sbjct: 113 FPSDLKGLAINNSDSIRAAHNSFARPEPFVPEEQKAATKDDDVYHFISYIPVDGVLYELD 172
Query: 695 GRK 703
G K
Sbjct: 173 GLK 175
>At5g16310.1 68418.m01907 ubiquitin carboxyl-terminal hydrolase
family 1 protein similar to 26S proteasome regulatory
complex subunit p37A [Drosophila melanogaster]
GI:6434962; contains Pfam profile PF01088: Ubiquitin
carboxyl-terminal hydrolase, family 1
Length = 334
Score = 62.5 bits (145), Expect = 3e-10
Identities = 52/190 (27%), Positives = 93/190 (48%), Gaps = 10/190 (5%)
Frame = +2
Query: 164 VPLESNPDVLNKFLQKLGVPNKWNIVDVMGLDPETLSWVPRPVLSVMLLFPISDAYENHK 343
+P+ES+P + + +Q++ V + ++ LD +L + RPV ++LL Y+
Sbjct: 4 LPVESDPGIFTEIIQQMQVKGV-QVEELYSLDFNSLDEI-RPVYGLILL------YKWRP 55
Query: 344 KTEENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANNTDIIELSDGHMQKFLNEAK 523
+ +EN ++ E + N F+ Q I+NAC T A++ SV N+ I++ + + AK
Sbjct: 56 EEKENRVVIT--EPNPNFFFASQIINNACATQAIL-SVLMNSSSIDIGS-ELSELKQFAK 111
Query: 524 GLDATARGKLLEKSEGIINAHKELAQEGQTNTPSAE----------DPVNHHFISFVQKD 673
+G + +E I AH A+ ++ E D +H+IS++ D
Sbjct: 112 EFPPELKGLAINNNEAIRAAHNTFARPDPSSIMEDEELAAAKNLDEDDDVYHYISYLPVD 171
Query: 674 GALYELNGRK 703
G LYEL+G K
Sbjct: 172 GILYELDGLK 181
>At1g65010.1 68414.m07368 expressed protein similar to
endosome-associated protein (GI:1016368) [Homo sapiens];
similar to Centromeric protein E (CENP-E protein)
(Swiss-Prot:Q02224) [Homo sapiens]
Length = 1318
Score = 40.7 bits (91), Expect = 0.001
Identities = 27/86 (31%), Positives = 44/86 (51%)
Frame = +2
Query: 341 KKTEENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANNTDIIELSDGHMQKFLNEA 520
KK+EE E S +EVS + +K++ +AC S+ NN + E ++Q+ L EA
Sbjct: 522 KKSEE-ENSSSQEEVSRLVNLLKESEEDACARKEEEASLKNNLKVAEGEVKYLQETLGEA 580
Query: 521 KGLDATARGKLLEKSEGIINAHKELA 598
K + LL+K E + N E++
Sbjct: 581 KAESMKLKESLLDKEEDLKNVTAEIS 606
>At5g10500.1 68418.m01216 kinase interacting family protein similar
to kinase interacting protein 1 (GI:13936326) [Petunia
integrifolia]
Length = 848
Score = 30.7 bits (66), Expect = 0.99
Identities = 20/79 (25%), Positives = 39/79 (49%), Gaps = 2/79 (2%)
Frame = +2
Query: 440 ALVHSVANNTDIIELSDGHMQKFLNEAKGLDATARGKLLEKSEGIINAHKELAQEGQTNT 619
AL+H + D ++ +QK N ++ D GK L++ E +N K++ QE + +
Sbjct: 358 ALIHRLREEIDDLKAQIRALQKENNSSQTDDNMDMGKKLKEMEEKVNGVKDIDQEVEEKS 417
Query: 620 PSAEDPVN--HHFISFVQK 670
+ + + H +SF+ K
Sbjct: 418 DNIDKHLTRAHMKLSFLSK 436
>At3g02930.1 68416.m00288 expressed protein ; expression supported
by MPSS
Length = 806
Score = 28.7 bits (61), Expect = 4.0
Identities = 24/92 (26%), Positives = 46/92 (50%), Gaps = 5/92 (5%)
Frame = +2
Query: 335 NHKKTEENEILSKGQEVS--GNIFYMKQNISNACGTIALVHSVANNTDIIELSDGHMQKF 508
NH K + E+ S G+E++ GN+ +K+ A + + + +E ++Q+
Sbjct: 543 NHVKEFDEEVSSMGKEMNRLGNL--VKRTKEEADASWEKESQMRDCLKEVEDEVIYLQET 600
Query: 509 LNEAKGLDATARGKLLEKS---EGIINAHKEL 595
L EAK +GK+L+K + I++ + EL
Sbjct: 601 LREAKAETLKLKGKMLDKETEFQSIVHENDEL 632
>At2g19910.1 68415.m02327 RNA-dependent RNA polymerase family
protein contains Pfam domain, PF05183: RNA dependent RNA
polymerase
Length = 992
Score = 27.9 bits (59), Expect = 7.0
Identities = 13/55 (23%), Positives = 26/55 (47%), Gaps = 1/55 (1%)
Frame = -2
Query: 312 KSSITESTGRGTHESVSGSSPMTSTIFHLFGTPSFCKNLFRT-SGLDSRGTKVSV 151
K + E+TG H+ + + +T + G ++C +L+ T G+ G V +
Sbjct: 229 KGHLLENTGTHLHKVLGDDNVLTVKFDKVLGVETYCNDLYSTYKGIAKNGIMVGL 283
>At1g53020.1 68414.m06002 ubiquitin-conjugating enzyme family protein
similar to ubiquitin-conjugating enzyme GB:3319990 from
[Mus musculus]; contains Pfam profile PF00179:
Ubiquitin-conjugating enzyme
Length = 1163
Score = 27.9 bits (59), Expect = 7.0
Identities = 13/39 (33%), Positives = 23/39 (58%), Gaps = 2/39 (5%)
Frame = +3
Query: 567 KASLMLTRNWLKKAKQIHQVL--KIPSTIILSALSRKME 677
KAS ++NW+KK + ++L +P TI + A +M+
Sbjct: 909 KASKQHSKNWVKKVQDEWKILNQNLPETIFVRACESRMD 947
>At3g27590.1 68416.m03447 hypothetical protein
Length = 92
Score = 27.5 bits (58), Expect = 9.2
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = +2
Query: 515 EAKGLDATARGKLLEKSEGI 574
+A+GL ATARG+ L S G+
Sbjct: 5 DARGLPATARGRRLSSSSGV 24
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,412,819
Number of Sequences: 28952
Number of extensions: 314777
Number of successful extensions: 803
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 779
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 797
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1526202912
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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