BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0932 (707 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 34 0.11 At1g52560.1 68414.m05933 26.5 kDa class I small heat shock prote... 31 0.57 At1g19260.1 68414.m02396 hAT dimerisation domain-containing prot... 29 3.0 At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 29 4.0 At3g02420.1 68416.m00229 expressed protein 29 4.0 At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 29 4.0 At1g08560.1 68414.m00949 syntaxin-related protein KNOLLE (KN) / ... 28 5.3 At5g55300.1 68418.m06891 DNA topoisomerase I identical to Swiss-... 28 7.0 At4g31130.1 68417.m04419 expressed protein 27 9.2 At3g29765.1 68416.m03762 hAT dimerisation domain-containing prot... 27 9.2 At2g14247.1 68415.m01591 Expressed protein 27 9.2 >At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HSP18.1-CI) identical to 18.2 kDa class I heat shock protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana]; contains Pfam profile: PF00011 Hsp20/alpha crystallin family Length = 161 Score = 33.9 bits (74), Expect = 0.11 Identities = 22/66 (33%), Positives = 38/66 (57%), Gaps = 8/66 (12%) Frame = +1 Query: 265 DEIKVTVKNKYIT-VEGKHKEAGDIK-------KFLTNHFVQRFVLPPGSKQEEVRAIYK 420 +E+KV V++K + + G+ + + K + + F++RF LP +K EEV+A Sbjct: 75 EEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKMEEVKAT-M 133 Query: 421 ENGILT 438 ENG+LT Sbjct: 134 ENGVLT 139 >At1g52560.1 68414.m05933 26.5 kDa class I small heat shock protein-like (HSP26.5-P) contains Pfam profile: PF00011 Hsp20/alpha crystallin family; identified in Scharf, K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237. Length = 232 Score = 31.5 bits (68), Expect = 0.57 Identities = 22/86 (25%), Positives = 46/86 (53%), Gaps = 9/86 (10%) Frame = +1 Query: 205 QIIANDDKLEVNMNVKRFSPDEIKVTVKNKYITVEGKHK---EAG--DIKKFLTN----H 357 Q+ DD ++ V + +++K+TV + +T++G HK E G + ++ ++ + Sbjct: 128 QVKEQDDCYKLRYEVPGLTKEDVKITVNDGILTIKGDHKAEEEKGSPEEDEYWSSKSYGY 187 Query: 358 FVQRFVLPPGSKQEEVRAIYKENGIL 435 + LP +K E+++A K NG+L Sbjct: 188 YNTSLSLPDDAKVEDIKAELK-NGVL 212 >At1g19260.1 68414.m02396 hAT dimerisation domain-containing protein Length = 769 Score = 29.1 bits (62), Expect = 3.0 Identities = 18/50 (36%), Positives = 26/50 (52%) Frame = -2 Query: 436 LIYRFLCKSLALLPVCCRAVKRISERNDSSRTSLCRRLPYAFLRP*CICF 287 L+YR L SL +LPV V+R + +T+L R+ FL +CF Sbjct: 693 LVYRLLKVSL-ILPVATATVERCFSAMNFVKTTLRNRIGDMFLSDCLVCF 741 >At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HSP17.4-CI) identical to 17.4 kDa class I heat shock protein SP:P19036 from [Arabidopsis thaliana] Length = 156 Score = 28.7 bits (61), Expect = 4.0 Identities = 14/27 (51%), Positives = 20/27 (74%) Frame = +1 Query: 358 FVQRFVLPPGSKQEEVRAIYKENGILT 438 F++RF LP +K EEV+A ENG+L+ Sbjct: 111 FMRRFRLPENAKVEEVKA-SMENGVLS 136 >At3g02420.1 68416.m00229 expressed protein Length = 348 Score = 28.7 bits (61), Expect = 4.0 Identities = 11/34 (32%), Positives = 24/34 (70%), Gaps = 2/34 (5%) Frame = -2 Query: 421 LCKSLALLPVCCRAVKRISE--RNDSSRTSLCRR 326 LC AL+P+ CRA++++++ R + R+++ R+ Sbjct: 215 LCLKFALIPILCRALEQVAKFLRRNFGRSTIYRK 248 >At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein (HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853) (GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res. 17 (19), 7995 (1989)) Length = 157 Score = 28.7 bits (61), Expect = 4.0 Identities = 13/27 (48%), Positives = 19/27 (70%) Frame = +1 Query: 358 FVQRFVLPPGSKQEEVRAIYKENGILT 438 F +RF LP +K EE++A ENG+L+ Sbjct: 112 FTRRFRLPENAKMEEIKA-SMENGVLS 137 >At1g08560.1 68414.m00949 syntaxin-related protein KNOLLE (KN) / syntaxin 111 (SYP111) identical to SP|Q42374 Syntaxin-related protein KNOLLE (Syntaxin 111) (AtSYP111) {Arabidopsis thaliana}; BAC F22O13 has a deletion of a cytosine at position 7887 Length = 310 Score = 28.3 bits (60), Expect = 5.3 Identities = 12/30 (40%), Positives = 19/30 (63%) Frame = +1 Query: 268 EIKVTVKNKYITVEGKHKEAGDIKKFLTNH 357 E K TV+ +Y TV G+H I+K +T++ Sbjct: 160 EYKETVERRYFTVTGEHANDEMIEKIITDN 189 >At5g55300.1 68418.m06891 DNA topoisomerase I identical to Swiss-Prot:P30181 DNA topoisomerase I [Arabidopsis thaliana] Length = 916 Score = 27.9 bits (59), Expect = 7.0 Identities = 15/49 (30%), Positives = 22/49 (44%) Frame = +1 Query: 274 KVTVKNKYITVEGKHKEAGDIKKFLTNHFVQRFVLPPGSKQEEVRAIYK 420 KVT +KY T +GD +K T + PP K ++ +YK Sbjct: 347 KVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKILYK 395 >At4g31130.1 68417.m04419 expressed protein Length = 195 Score = 27.5 bits (58), Expect = 9.2 Identities = 12/24 (50%), Positives = 16/24 (66%) Frame = -1 Query: 329 PASLCFPSTVMYLFFTVTLISSGL 258 P S+ F ST +FF + LI+SGL Sbjct: 84 PNSVLFKSTSFSVFFNIALITSGL 107 >At3g29765.1 68416.m03762 hAT dimerisation domain-containing protein Length = 536 Score = 27.5 bits (58), Expect = 9.2 Identities = 18/50 (36%), Positives = 24/50 (48%) Frame = -2 Query: 436 LIYRFLCKSLALLPVCCRAVKRISERNDSSRTSLCRRLPYAFLRP*CICF 287 L+YR L SL +LPV V R + +T+L R FL +CF Sbjct: 460 LVYRLLKLSL-ILPVATATVVRCFSAMNFVKTTLRNRFGDVFLSDCLVCF 508 >At2g14247.1 68415.m01591 Expressed protein Length = 78 Score = 27.5 bits (58), Expect = 9.2 Identities = 9/29 (31%), Positives = 16/29 (55%) Frame = +1 Query: 70 HKVEDPFVALDKHVSNTLAYHYLWPWSQL 156 HK ++ ++ V +T + +LWPW L Sbjct: 17 HKAKNQSLSSPSSVKSTRVFGFLWPWKAL 45 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.314 0.131 0.379 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,207,008 Number of Sequences: 28952 Number of extensions: 251418 Number of successful extensions: 464 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 459 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 464 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1526202912 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 42 (22.0 bits)
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