Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0920
(719 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharom... 250 1e-67
SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyc... 31 0.22
SPBC23G7.06c |||conserved eukaryotic protein|Schizosaccharomyces... 29 0.67
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 28 1.2
SPAC3A11.05c |kms1||meiotic spindle pole body protein Kms1|Schiz... 27 2.0
SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosacch... 27 3.6
SPAC19G12.05 |||mitochondrial citrate transporter|Schizosaccharo... 26 4.7
SPAC22A12.02c |mug103||sequence orphan|Schizosaccharomyces pombe... 26 6.2
SPCC970.07c |raf2|dos2, cmc2, clr7|Rik1-associated factor Raf2|S... 26 6.2
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 6.2
SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces ... 26 6.2
>SPAC926.04c |hsp90|swo1|heat shock protein
Hsp90|Schizosaccharomyces pombe|chr 1|||Manual
Length = 704
Score = 250 bits (613), Expect = 1e-67
Identities = 111/137 (81%), Positives = 128/137 (93%)
Frame = +1
Query: 307 LNKTKPIWTRNADDITQDEYGDFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFVPRRAPF 486
LNKTKPIWTRN ++T++EY FYKSLTNDWEDHLAVKHFSVEGQLEFRA+LFVPRRAP
Sbjct: 265 LNKTKPIWTRNPSEVTKEEYASFYKSLTNDWEDHLAVKHFSVEGQLEFRAILFVPRRAPM 324
Query: 487 DLFENKKRKNNIKLYVRRVFIMDNCEDLIPEYLNFIRGVVDSEDLPLNISREMLQQNKIL 666
DLFE K++KNNIKLYVRRVFI D+CE+LIPE+L FI+GVVDSEDLPLN+SREMLQQNKI+
Sbjct: 325 DLFEAKRKKNNIKLYVRRVFITDDCEELIPEWLGFIKGVVDSEDLPLNLSREMLQQNKIM 384
Query: 667 KVIRKNLVKKCLELFEE 717
KVIRKNLV++CL++F E
Sbjct: 385 KVIRKNLVRRCLDMFNE 401
Score = 42.3 bits (95), Expect = 7e-05
Identities = 19/26 (73%), Positives = 22/26 (84%)
Frame = +1
Query: 100 AEVETFAFQAEIAQLMSLIINTFYSN 177
+ ETF F+AEI+QLMSLIINT YSN
Sbjct: 2 SNTETFKFEAEISQLMSLIINTVYSN 27
>SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1679
Score = 30.7 bits (66), Expect = 0.22
Identities = 29/125 (23%), Positives = 55/125 (44%), Gaps = 2/125 (1%)
Frame = +1
Query: 334 RNADDITQDEYGDFYKSLTN-DWEDHLAVKHFSVEG-QLEFRALLFVPRRAPFDLFENKK 507
R AD+ T+D ++L++ + +A+ F +E F + + +A L ++K
Sbjct: 212 RLADEFTRDSGLPDLENLSHVNVLLDIALDKFRIEALHANFSVSISLRNKAIQHLLKSKI 271
Query: 508 RKNNIKLYVRRVFIMDNCEDLIPEYLNFIRGVVDSEDLPLNISREMLQQNKILKVIRKNL 687
N K FI++ + P + F R VVD D + + KIL ++ N+
Sbjct: 272 AANTDKAINCIEFILEAPPSMQPRLIQFTRWVVDKADPNFLKPKAAMILEKILSILSSNI 331
Query: 688 VKKCL 702
++ L
Sbjct: 332 IQSDL 336
>SPBC23G7.06c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 745
Score = 29.1 bits (62), Expect = 0.67
Identities = 21/71 (29%), Positives = 36/71 (50%), Gaps = 2/71 (2%)
Frame = +2
Query: 419 NTSPLKVS*SSEHYFLCHVVHLLTCLKTRS--ARTISSCMYEEFSSWTTVRISSQSTLTS 592
NTSPLK + S+ F+ V L LKT++ R+I + S TT + S + +
Sbjct: 514 NTSPLKYTHSASKSFIGEVQDSLQALKTKAHKPRSIGGDSSQTTLSETTKKYGSVAKKSF 573
Query: 593 LEVLSTVRTFL 625
+ +S ++F+
Sbjct: 574 FQGVSDAKSFV 584
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 28.3 bits (60), Expect = 1.2
Identities = 16/44 (36%), Positives = 26/44 (59%)
Frame = +2
Query: 320 SLSGPETLMTLPKMSTETSTNPLPMIGRTILQSNTSPLKVS*SS 451
SLS P T T+P ST +++ P+P + +N+SPL + +S
Sbjct: 186 SLSTPIT-PTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTSTS 228
Score = 28.3 bits (60), Expect = 1.2
Identities = 16/44 (36%), Positives = 26/44 (59%)
Frame = +2
Query: 320 SLSGPETLMTLPKMSTETSTNPLPMIGRTILQSNTSPLKVS*SS 451
SLS P T T+P ST +++ P+P + +N+SPL + +S
Sbjct: 242 SLSTPIT-PTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTSTS 284
Score = 27.1 bits (57), Expect = 2.7
Identities = 15/44 (34%), Positives = 26/44 (59%)
Frame = +2
Query: 320 SLSGPETLMTLPKMSTETSTNPLPMIGRTILQSNTSPLKVS*SS 451
SLS P T T+P ST +++ P+P + +N++PL + +S
Sbjct: 129 SLSTPIT-PTVPPTSTSSTSIPIPPTSTSSTDTNSNPLPTTSTS 171
>SPAC3A11.05c |kms1||meiotic spindle pole body protein
Kms1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 607
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/28 (46%), Positives = 18/28 (64%), Gaps = 1/28 (3%)
Frame = +1
Query: 334 RNAD-DITQDEYGDFYKSLTNDWEDHLA 414
+NAD IT+D + D YK LT + +D A
Sbjct: 60 QNADLSITKDNFRDVYKKLTENEDDSFA 87
>SPBC13G1.10c |mug81||ATP-dependent RNA helicase
Slh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1935
Score = 26.6 bits (56), Expect = 3.6
Identities = 19/72 (26%), Positives = 34/72 (47%)
Frame = +1
Query: 394 DWEDHLAVKHFSVEGQLEFRALLFVPRRAPFDLFENKKRKNNIKLYVRRVFIMDNCEDLI 573
D LAVK + G L L + DL N+K +K ++ ++ +++ DL+
Sbjct: 939 DLPHDLAVKVENQCGSLSLEELSDMSTGELGDLIHNRKMGPTVKKFISKLPLLNINVDLL 998
Query: 574 PEYLNFIRGVVD 609
P N +R V++
Sbjct: 999 PLTKNVLRLVLN 1010
>SPAC19G12.05 |||mitochondrial citrate
transporter|Schizosaccharomyces pombe|chr 1|||Manual
Length = 291
Score = 26.2 bits (55), Expect = 4.7
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +2
Query: 542 FSSWTTVRISSQSTLTSLEVLSTVRTFL 625
F+++ +++ S QS L E LSTV TFL
Sbjct: 181 FTAYNSIKQSLQSRLPPDEKLSTVTTFL 208
>SPAC22A12.02c |mug103||sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 124
Score = 25.8 bits (54), Expect = 6.2
Identities = 12/31 (38%), Positives = 22/31 (70%)
Frame = +1
Query: 604 VDSEDLPLNISREMLQQNKILKVIRKNLVKK 696
++SED+PL+ R+ML++ + RK L+K+
Sbjct: 87 INSEDMPLSDDRDMLKERHVKS--RKLLLKE 115
>SPCC970.07c |raf2|dos2, cmc2, clr7|Rik1-associated factor
Raf2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 636
Score = 25.8 bits (54), Expect = 6.2
Identities = 15/76 (19%), Positives = 33/76 (43%)
Frame = +1
Query: 490 LFENKKRKNNIKLYVRRVFIMDNCEDLIPEYLNFIRGVVDSEDLPLNISREMLQQNKILK 669
++E KN + ++ F D + +Y N+I + + +LP +L Q I
Sbjct: 108 IYEQISEKNRLFYLIKTKFKDDMISGTLEDYDNYIEVLKEKLELPSCFQAILLVQKHIRF 167
Query: 670 VIRKNLVKKCLELFEE 717
++ + + L ++ E
Sbjct: 168 LLTQMVATSSLHVWSE 183
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.8 bits (54), Expect = 6.2
Identities = 14/41 (34%), Positives = 23/41 (56%), Gaps = 5/41 (12%)
Frame = +1
Query: 490 LFENKKRKNNIK-----LYVRRVFIMDNCEDLIPEYLNFIR 597
L EN KNN+ L ++ I++ CE +I + LNF++
Sbjct: 1144 LQENTSSKNNLLQKEWILITKKKVILEQCEYVILDILNFLK 1184
>SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 2493
Score = 25.8 bits (54), Expect = 6.2
Identities = 13/52 (25%), Positives = 28/52 (53%)
Frame = +1
Query: 556 NCEDLIPEYLNFIRGVVDSEDLPLNISREMLQQNKILKVIRKNLVKKCLELF 711
+C ++ E + + D+E+ N+ R++L ++ KNLV+ ++LF
Sbjct: 2122 HCREMSAELIKLVYQRADNENF--NLIRQLLSHWTSVEKAGKNLVRVSMQLF 2171
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,392,875
Number of Sequences: 5004
Number of extensions: 44076
Number of successful extensions: 139
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 130
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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