Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0920
(719 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 247 7e-66
At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearl... 244 4e-65
At5g56010.1 68418.m06989 heat shock protein, putative strong sim... 244 4e-65
At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearl... 243 8e-65
At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata format... 155 4e-38
At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata format... 155 4e-38
At2g04030.2 68415.m00372 heat shock protein, putative strong sim... 148 3e-36
At2g04030.1 68415.m00371 heat shock protein, putative strong sim... 148 3e-36
At3g07770.1 68416.m00947 heat shock protein-related strong simil... 145 3e-35
At5g44750.1 68418.m05485 UMUC-like DNA repair family protein / B... 34 0.082
At3g50660.1 68416.m05541 steroid 22-alpha-hydroxylase (CYP90B1) ... 33 0.25
At3g13530.1 68416.m01701 MAP3K epsilon protein kinase identical ... 28 5.4
At2g01520.1 68415.m00076 major latex protein-related / MLP-relat... 28 7.2
At5g54660.1 68418.m06806 heat shock protein-related contains wea... 27 9.5
>At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat
shock protein 83 (HSP83) nearly identical to SP|P27323
Heat shock protein 81-1 (HSP81-1) (Heat shock protein
83) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 705
Score = 247 bits (604), Expect = 7e-66
Identities = 109/137 (79%), Positives = 127/137 (92%)
Frame = +1
Query: 307 LNKTKPIWTRNADDITQDEYGDFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFVPRRAPF 486
+NK KPIW R ++IT++EY FYKSLTNDWEDHLAVKHFSVEGQLEF+A+LFVP+RAPF
Sbjct: 267 INKQKPIWLRKPEEITKEEYAAFYKSLTNDWEDHLAVKHFSVEGQLEFKAILFVPKRAPF 326
Query: 487 DLFENKKRKNNIKLYVRRVFIMDNCEDLIPEYLNFIRGVVDSEDLPLNISREMLQQNKIL 666
DLF+ +K+ NNIKLYVRRVFIMDNCE+LIPEYL+F++GVVDS+DLPLNISRE LQQNKIL
Sbjct: 327 DLFDTRKKLNNIKLYVRRVFIMDNCEELIPEYLSFVKGVVDSDDLPLNISRETLQQNKIL 386
Query: 667 KVIRKNLVKKCLELFEE 717
KVIRKNLVKKC+E+F E
Sbjct: 387 KVIRKNLVKKCIEMFNE 403
Score = 50.0 bits (114), Expect = 2e-06
Identities = 23/30 (76%), Positives = 26/30 (86%)
Frame = +1
Query: 88 ETQPAEVETFAFQAEIAQLMSLIINTFYSN 177
+ Q A+ ETFAFQAEI QL+SLIINTFYSN
Sbjct: 3 DVQMADAETFAFQAEINQLLSLIINTFYSN 32
>At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearly
identical to SP|P55737 Heat shock protein 81-2 (HSP81-2)
{Arabidopsis thaliana}
Length = 699
Score = 244 bits (598), Expect = 4e-65
Identities = 109/137 (79%), Positives = 125/137 (91%)
Frame = +1
Query: 307 LNKTKPIWTRNADDITQDEYGDFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFVPRRAPF 486
+NK KPIW R ++I ++EY FYKSL+NDWE+HLAVKHFSVEGQLEF+A+LFVP+RAPF
Sbjct: 261 VNKQKPIWMRKPEEINKEEYAAFYKSLSNDWEEHLAVKHFSVEGQLEFKAILFVPKRAPF 320
Query: 487 DLFENKKRKNNIKLYVRRVFIMDNCEDLIPEYLNFIRGVVDSEDLPLNISREMLQQNKIL 666
DLF+ KK+ NNIKLYVRRVFIMDNCED+IPEYL F++G+VDSEDLPLNISRE LQQNKIL
Sbjct: 321 DLFDTKKKPNNIKLYVRRVFIMDNCEDIIPEYLGFVKGIVDSEDLPLNISRETLQQNKIL 380
Query: 667 KVIRKNLVKKCLELFEE 717
KVIRKNLVKKCLELF E
Sbjct: 381 KVIRKNLVKKCLELFFE 397
Score = 48.0 bits (109), Expect = 6e-06
Identities = 22/26 (84%), Positives = 24/26 (92%)
Frame = +1
Query: 100 AEVETFAFQAEIAQLMSLIINTFYSN 177
A+ ETFAFQAEI QL+SLIINTFYSN
Sbjct: 2 ADAETFAFQAEINQLLSLIINTFYSN 27
>At5g56010.1 68418.m06989 heat shock protein, putative strong
similarity to SP|P55737 Heat shock protein 81-2
(HSP81-2) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 699
Score = 244 bits (598), Expect = 4e-65
Identities = 109/137 (79%), Positives = 125/137 (91%)
Frame = +1
Query: 307 LNKTKPIWTRNADDITQDEYGDFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFVPRRAPF 486
+NK KPIW R ++I ++EY FYKSL+NDWE+HLAVKHFSVEGQLEF+A+LFVP+RAPF
Sbjct: 261 VNKQKPIWMRKPEEINKEEYAAFYKSLSNDWEEHLAVKHFSVEGQLEFKAILFVPKRAPF 320
Query: 487 DLFENKKRKNNIKLYVRRVFIMDNCEDLIPEYLNFIRGVVDSEDLPLNISREMLQQNKIL 666
DLF+ KK+ NNIKLYVRRVFIMDNCED+IPEYL F++G+VDSEDLPLNISRE LQQNKIL
Sbjct: 321 DLFDTKKKPNNIKLYVRRVFIMDNCEDIIPEYLGFVKGIVDSEDLPLNISRETLQQNKIL 380
Query: 667 KVIRKNLVKKCLELFEE 717
KVIRKNLVKKCLELF E
Sbjct: 381 KVIRKNLVKKCLELFFE 397
Score = 48.0 bits (109), Expect = 6e-06
Identities = 22/26 (84%), Positives = 24/26 (92%)
Frame = +1
Query: 100 AEVETFAFQAEIAQLMSLIINTFYSN 177
A+ ETFAFQAEI QL+SLIINTFYSN
Sbjct: 2 ADAETFAFQAEINQLLSLIINTFYSN 27
>At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearly
identical to heat shock protein hsp81.4 [Arabidopsis
thaliana] GI:1906828; contains Pfam profiles PF02518:
ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like
domain protein, PF00183: Hsp90 protein
Length = 699
Score = 243 bits (595), Expect = 8e-65
Identities = 108/137 (78%), Positives = 125/137 (91%)
Frame = +1
Query: 307 LNKTKPIWTRNADDITQDEYGDFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFVPRRAPF 486
+NK KPIW R ++I ++EY FYKSL+NDWE+HLAVKHFSVEGQLEF+A+LFVP+RAPF
Sbjct: 261 VNKQKPIWMRKPEEINKEEYAAFYKSLSNDWEEHLAVKHFSVEGQLEFKAILFVPKRAPF 320
Query: 487 DLFENKKRKNNIKLYVRRVFIMDNCEDLIPEYLNFIRGVVDSEDLPLNISREMLQQNKIL 666
DLF+ KK+ NNIKLYVRRVFIMDNCED+IP+YL F++G+VDSEDLPLNISRE LQQNKIL
Sbjct: 321 DLFDTKKKPNNIKLYVRRVFIMDNCEDIIPDYLGFVKGIVDSEDLPLNISRETLQQNKIL 380
Query: 667 KVIRKNLVKKCLELFEE 717
KVIRKNLVKKCLELF E
Sbjct: 381 KVIRKNLVKKCLELFFE 397
Score = 48.0 bits (109), Expect = 6e-06
Identities = 22/26 (84%), Positives = 24/26 (92%)
Frame = +1
Query: 100 AEVETFAFQAEIAQLMSLIINTFYSN 177
A+ ETFAFQAEI QL+SLIINTFYSN
Sbjct: 2 ADAETFAFQAEINQLLSLIINTFYSN 27
>At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 155 bits (375), Expect = 4e-38
Identities = 70/141 (49%), Positives = 104/141 (73%), Gaps = 4/141 (2%)
Frame = +1
Query: 307 LNKTKPIWTRNADDITQDEYGDFYKSLTNDWEDH--LAVKHFSVEGQLEFRALLFVPRRA 480
LN K IW R+ ++T++EY FY SL+ D+ D +A HF+ EG +EF+A+L+VP +A
Sbjct: 341 LNDVKAIWLRSPKEVTEEEYTKFYHSLSKDFTDEKPMAWSHFNAEGDVEFKAVLYVPPKA 400
Query: 481 PFDLFEN--KKRKNNIKLYVRRVFIMDNCEDLIPEYLNFIRGVVDSEDLPLNISREMLQQ 654
P DL+E+ K N+KLYVRRVFI D ++L+P+YL+F++G+VDS+ LPLN+SREMLQQ
Sbjct: 401 PHDLYESYYNSNKANLKLYVRRVFISDEFDELLPKYLSFLKGLVDSDTLPLNVSREMLQQ 460
Query: 655 NKILKVIRKNLVKKCLELFEE 717
+ LK I+K L++K L++ +
Sbjct: 461 HSSLKTIKKKLIRKALDMIRK 481
Score = 36.3 bits (80), Expect = 0.020
Identities = 15/30 (50%), Positives = 22/30 (73%)
Frame = +1
Query: 88 ETQPAEVETFAFQAEIAQLMSLIINTFYSN 177
+T + E F FQAE+++LM +IIN+ YSN
Sbjct: 70 KTLRSNAEKFEFQAEVSRLMDIIINSLYSN 99
>At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 155 bits (375), Expect = 4e-38
Identities = 70/141 (49%), Positives = 104/141 (73%), Gaps = 4/141 (2%)
Frame = +1
Query: 307 LNKTKPIWTRNADDITQDEYGDFYKSLTNDWEDH--LAVKHFSVEGQLEFRALLFVPRRA 480
LN K IW R+ ++T++EY FY SL+ D+ D +A HF+ EG +EF+A+L+VP +A
Sbjct: 341 LNDVKAIWLRSPKEVTEEEYTKFYHSLSKDFTDEKPMAWSHFNAEGDVEFKAVLYVPPKA 400
Query: 481 PFDLFEN--KKRKNNIKLYVRRVFIMDNCEDLIPEYLNFIRGVVDSEDLPLNISREMLQQ 654
P DL+E+ K N+KLYVRRVFI D ++L+P+YL+F++G+VDS+ LPLN+SREMLQQ
Sbjct: 401 PHDLYESYYNSNKANLKLYVRRVFISDEFDELLPKYLSFLKGLVDSDTLPLNVSREMLQQ 460
Query: 655 NKILKVIRKNLVKKCLELFEE 717
+ LK I+K L++K L++ +
Sbjct: 461 HSSLKTIKKKLIRKALDMIRK 481
Score = 36.3 bits (80), Expect = 0.020
Identities = 15/30 (50%), Positives = 22/30 (73%)
Frame = +1
Query: 88 ETQPAEVETFAFQAEIAQLMSLIINTFYSN 177
+T + E F FQAE+++LM +IIN+ YSN
Sbjct: 70 KTLRSNAEKFEFQAEVSRLMDIIINSLYSN 99
>At2g04030.2 68415.m00372 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 777
Score = 148 bits (359), Expect = 3e-36
Identities = 65/139 (46%), Positives = 106/139 (76%), Gaps = 3/139 (2%)
Frame = +1
Query: 310 NKTKPIWTRNADDITQDEYGDFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFVPRRAPFD 489
N+TKP+W RN+ ++ + EY +FYK N++ D LA HF+ EG++EFR++L++P P +
Sbjct: 330 NETKPLWMRNSKEVEKGEYNEFYKKAFNEFLDPLAHTHFTTEGEVEFRSILYIPGMGPLN 389
Query: 490 LFE--NKKRKNNIKLYVRRVFIMDNCE-DLIPEYLNFIRGVVDSEDLPLNISREMLQQNK 660
+ N K KN I+LYV+RVFI D+ + +L P YL+F++GVVDS+DLPLN+SRE+LQ+++
Sbjct: 390 NEDVTNPKTKN-IRLYVKRVFISDDFDGELFPRYLSFVKGVVDSDDLPLNVSREILQESR 448
Query: 661 ILKVIRKNLVKKCLELFEE 717
I++++RK L++K ++ +E
Sbjct: 449 IVRIMRKRLIRKTFDMIQE 467
Score = 31.5 bits (68), Expect = 0.58
Identities = 12/33 (36%), Positives = 23/33 (69%)
Frame = +1
Query: 79 EEMETQPAEVETFAFQAEIAQLMSLIINTFYSN 177
E+ T+ E F +QAE+++L+ LI+++ YS+
Sbjct: 67 EKETTEEGSGEKFEYQAEVSRLLDLIVHSLYSH 99
>At2g04030.1 68415.m00371 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 780
Score = 148 bits (359), Expect = 3e-36
Identities = 65/139 (46%), Positives = 106/139 (76%), Gaps = 3/139 (2%)
Frame = +1
Query: 310 NKTKPIWTRNADDITQDEYGDFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFVPRRAPFD 489
N+TKP+W RN+ ++ + EY +FYK N++ D LA HF+ EG++EFR++L++P P +
Sbjct: 330 NETKPLWMRNSKEVEKGEYNEFYKKAFNEFLDPLAHTHFTTEGEVEFRSILYIPGMGPLN 389
Query: 490 LFE--NKKRKNNIKLYVRRVFIMDNCE-DLIPEYLNFIRGVVDSEDLPLNISREMLQQNK 660
+ N K KN I+LYV+RVFI D+ + +L P YL+F++GVVDS+DLPLN+SRE+LQ+++
Sbjct: 390 NEDVTNPKTKN-IRLYVKRVFISDDFDGELFPRYLSFVKGVVDSDDLPLNVSREILQESR 448
Query: 661 ILKVIRKNLVKKCLELFEE 717
I++++RK L++K ++ +E
Sbjct: 449 IVRIMRKRLIRKTFDMIQE 467
Score = 31.5 bits (68), Expect = 0.58
Identities = 12/33 (36%), Positives = 23/33 (69%)
Frame = +1
Query: 79 EEMETQPAEVETFAFQAEIAQLMSLIINTFYSN 177
E+ T+ E F +QAE+++L+ LI+++ YS+
Sbjct: 67 EKETTEEGSGEKFEYQAEVSRLLDLIVHSLYSH 99
>At3g07770.1 68416.m00947 heat shock protein-related strong
similarity to heat-shock protein [Secale cereale]
GI:556673; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 803
Score = 145 bits (351), Expect = 3e-35
Identities = 66/136 (48%), Positives = 103/136 (75%), Gaps = 3/136 (2%)
Frame = +1
Query: 310 NKTKPIWTRNADDITQDEYGDFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFVPRRAPF- 486
N+T+PIW RN ++T EY +FY+ N++ D LA HF+ EG++EFR++L+VP +P
Sbjct: 353 NETQPIWLRNPKEVTTAEYNEFYRKAFNEYLDPLASSHFTTEGEVEFRSILYVPPVSPSG 412
Query: 487 -DLFENKKRKNNIKLYVRRVFIMDNCE-DLIPEYLNFIRGVVDSEDLPLNISREMLQQNK 660
D N+K K NI+LYV+RVFI D+ + +L P YL+F++GVVDS DLPLN+SRE+LQ+++
Sbjct: 413 KDDIVNQKTK-NIRLYVKRVFISDDFDGELFPRYLSFVKGVVDSHDLPLNVSREILQESR 471
Query: 661 ILKVIRKNLVKKCLEL 708
I+++++K LV+K ++
Sbjct: 472 IVRIMKKRLVRKAFDM 487
Score = 36.3 bits (80), Expect = 0.020
Identities = 14/27 (51%), Positives = 20/27 (74%)
Frame = +1
Query: 97 PAEVETFAFQAEIAQLMSLIINTFYSN 177
P E F +QAE+++LM LI+N+ YSN
Sbjct: 91 PPPAEKFEYQAEVSRLMDLIVNSLYSN 117
>At5g44750.1 68418.m05485 UMUC-like DNA repair family protein / BRCT
domain-containing protein low similarity to Rev1S [Homo
sapiens] GI:12483635; contains Pfam profiles PF00817:
ImpB/MucB/SamB family, PF00533: BRCA1 C Terminus (BRCT)
domain
Length = 1102
Score = 34.3 bits (75), Expect = 0.082
Identities = 17/52 (32%), Positives = 26/52 (50%)
Frame = -1
Query: 509 RFLFSNKSKGARRGTKSSARNSN*PSTEKCLTARWSSQSLVRDL*KSPYSSW 354
R L SN S + G+ ++ +N PS +K L A W + S SP+S +
Sbjct: 3 RSLGSNSSNNSGSGSNKKSKKNNNPSNQKTLGAAWGAASSRSSFRSSPFSDF 54
>At3g50660.1 68416.m05541 steroid 22-alpha-hydroxylase (CYP90B1)
(DWF4) identical to gi:2935342
Length = 513
Score = 32.7 bits (71), Expect = 0.25
Identities = 23/80 (28%), Positives = 41/80 (51%), Gaps = 6/80 (7%)
Frame = +1
Query: 496 ENKKRKNNIKLYVRRVFIMD----NCEDLIPEYLNFIRGVVDSE-DLPLNISREMLQQN- 657
+++ +K L + + MD E L EY+ F++GVV + +LP + LQ
Sbjct: 177 QDEAKKFTFNLMAKHIMSMDPGEEETEQLKKEYVTFMKGVVSAPLNLPGTAYHKALQSRA 236
Query: 658 KILKVIRKNLVKKCLELFEE 717
ILK I + + ++ L++ EE
Sbjct: 237 TILKFIERKMEERKLDIKEE 256
>At3g13530.1 68416.m01701 MAP3K epsilon protein kinase identical to
MAP3K epsilon protein kinase [Arabidopsis thaliana]
gi|3549652|emb|CAA12272
Length = 1368
Score = 28.3 bits (60), Expect = 5.4
Identities = 18/61 (29%), Positives = 29/61 (47%)
Frame = +1
Query: 343 DDITQDEYGDFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFVPRRAPFDLFENKKRKNNI 522
D+I + YG YK L + D +A+K S+E ++ + DL +N KN +
Sbjct: 24 DEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIVQEDLNTIMQE---IDLLKNLNHKNIV 80
Query: 523 K 525
K
Sbjct: 81 K 81
>At2g01520.1 68415.m00076 major latex protein-related / MLP-related
low similarity to major latex protein {Papaver
somniferum}[GI:169000] contains Pfam profile PF00407:
Pathogenesis-related protein Bet v I family
Length = 151
Score = 27.9 bits (59), Expect = 7.2
Identities = 13/34 (38%), Positives = 18/34 (52%), Gaps = 1/34 (2%)
Frame = +1
Query: 313 KTKPIWTRNADDITQ-DEYGDFYKSLTNDWEDHL 411
K IW + DD+ + Y F KSL D +DH+
Sbjct: 115 KITMIWEKQNDDMPEPSNYMKFVKSLAADMDDHV 148
>At5g54660.1 68418.m06806 heat shock protein-related contains weak
similarity to 17.6 kDa class I heat shock protein (HSP
17.6) (Swiss-Prot:P13853) [Arabidopsis thaliana]
Length = 192
Score = 27.5 bits (58), Expect = 9.5
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = -3
Query: 426 EVFDCKMVLPIIGKGFVEVSVLILGNVISVSG 331
+ + K +P++GK V+V V I G V+ +SG
Sbjct: 96 QAYVLKSDIPVVGKNNVQVYVDINGRVMEISG 127
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,393,071
Number of Sequences: 28952
Number of extensions: 226062
Number of successful extensions: 711
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 672
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 701
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1565336320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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