Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0914
(680 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 38 0.008
At3g49540.1 68416.m05414 expressed protein 36 0.025
At3g23900.1 68416.m03003 RNA recognition motif (RRM)-containing ... 36 0.033
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 36 0.033
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 35 0.057
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 33 0.13
At4g15410.1 68417.m02355 UBX domain-containing protein low simil... 33 0.13
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 33 0.13
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 33 0.17
At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 30 1.2
At1g02840.3 68414.m00246 pre-mRNA splicing factor SF2 (SF2) / SR... 30 1.2
At1g02840.1 68414.m00245 pre-mRNA splicing factor SF2 (SF2) / SR... 30 1.2
At3g56100.1 68416.m06235 leucine-rich repeat transmembrane prote... 30 1.6
At4g14830.1 68417.m02280 expressed protein 29 2.8
At1g76770.1 68414.m08934 heat shock protein-related contains sim... 29 2.8
At2g34640.1 68415.m04255 expressed protein 29 3.8
At2g28680.1 68415.m03486 cupin family protein similar to legumin... 29 3.8
At2g20360.1 68415.m02377 expressed protein 28 5.0
At1g66720.1 68414.m07584 S-adenosyl-L-methionine:carboxyl methyl... 28 5.0
At4g22590.1 68417.m03259 trehalose-6-phosphate phosphatase, puta... 27 8.7
At1g66880.1 68414.m07601 serine/threonine protein kinase family ... 27 8.7
At1g60650.2 68414.m06828 glycine-rich RNA-binding protein, putat... 27 8.7
At1g60650.1 68414.m06827 glycine-rich RNA-binding protein, putat... 27 8.7
At1g05830.1 68414.m00610 trithorax protein, putative / PHD finge... 27 8.7
>At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein
(HSP22.0-ER) identical to endomembrane-localized small
heat shock protein GI:511795 from [Arabidopsis thaliana]
Length = 195
Score = 37.5 bits (83), Expect = 0.008
Identities = 20/48 (41%), Positives = 31/48 (64%)
Frame = +3
Query: 318 QFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 461
+F R++ LP+ E+V+++L + GVLTI + P+ VKG R V IA
Sbjct: 132 KFWRQFKLPDNVDMESVKAKLEN-GVLTINLTKLSPEKVKGPRVVNIA 178
>At3g49540.1 68416.m05414 expressed protein
Length = 166
Score = 35.9 bits (79), Expect = 0.025
Identities = 22/64 (34%), Positives = 37/64 (57%), Gaps = 4/64 (6%)
Frame = +3
Query: 345 EGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKV---PI-AQTGPVRKEIKDQSEEAN 512
E AA E E+++ + + + AP KV AV+ E+K P+ A+ PV+ E + EE+
Sbjct: 100 ENAATENAEAKVEA---VAVAAPEKVEVAVEAEKKAEAEPVKAEAEPVKAEAEPVKEESK 156
Query: 513 EKEK 524
++EK
Sbjct: 157 QEEK 160
>At3g23900.1 68416.m03003 RNA recognition motif (RRM)-containing
protein
Length = 987
Score = 35.5 bits (78), Expect = 0.033
Identities = 24/92 (26%), Positives = 43/92 (46%), Gaps = 6/92 (6%)
Frame = +1
Query: 292 KTSTGIFQGSSSDVTRCLKARRLRLWNRDCHQTGYSPSLR------RGRCPTPSRERERC 453
K++T + +++++R L+ + ++ Q SPS + + P R R R
Sbjct: 474 KSATELAAARAAEISRKLRPDGVGNDEKEADQKSRSPSKSPARSRSKSKSPISYRRRRRS 533
Query: 454 PSHRPVPFARRSRTRAKKPTRRKSRKVYEELR 549
P++ P PF R R++ P R + R YE R
Sbjct: 534 PTYSP-PFRRPRSHRSRSPLRYQRRSTYEGRR 564
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 35.5 bits (78), Expect = 0.033
Identities = 21/48 (43%), Positives = 25/48 (52%), Gaps = 5/48 (10%)
Frame = +1
Query: 397 SPSLRRGRCPTPSRERERCPS-----HRPVPFARRSRTRAKKPTRRKS 525
SP RR R PTP + R PS HR P ARR R+ + RR+S
Sbjct: 334 SPPARRHRSPTPPARQRRSPSPPARRHRSPPPARRRRSPSPPARRRRS 381
Score = 28.3 bits (60), Expect = 5.0
Identities = 23/82 (28%), Positives = 30/82 (36%)
Frame = +1
Query: 277 STKRRKTSTGIFQGSSSDVTRCLKARRLRLWNRDCHQTGYSPSLRRGRCPTPSRERERCP 456
S ++R+ S + S V R L RR R+ +SP R R P R
Sbjct: 248 SPRKRRLSNSRRRSRSRSVRRSLSPRRRRI---------HSPFRSRSRSPIRRHRRPTHE 298
Query: 457 SHRPVPFARRSRTRAKKPTRRK 522
R P R R P RR+
Sbjct: 299 GRRQSPAPSRRRRSPSPPARRR 320
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 34.7 bits (76), Expect = 0.057
Identities = 18/50 (36%), Positives = 31/50 (62%)
Frame = +3
Query: 312 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 461
S +F RR+ LPE A E +++ + +GVL++T P KVP+ + + I+
Sbjct: 109 SGKFTRRFRLPENAKMEEIKASM-ENGVLSVTVP-KVPEKKPEVKSIDIS 156
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 33.5 bits (73), Expect = 0.13
Identities = 18/50 (36%), Positives = 30/50 (60%)
Frame = +3
Query: 312 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 461
S +F+RR+ LPE A E V++ + +GVLT+ P K P+ + + I+
Sbjct: 111 SGKFMRRFRLPENAKMEEVKATM-ENGVLTVVVP-KAPEKKPQVKSIDIS 158
>At4g15410.1 68417.m02355 UBX domain-containing protein low
similarity to XY40 protein [Rattus norvegicus]
GI:2547025; contains Pfam profile PF00789: UBX domain
Length = 421
Score = 33.5 bits (73), Expect = 0.13
Identities = 21/65 (32%), Positives = 28/65 (43%)
Frame = +1
Query: 382 HQTGYSPSLRRGRCPTPSRERERCPSHRPVPFARRSRTRAKKPTRRKSRKVYEELRLLQN 561
H Y+PS P+PSR R PS R P+ RSR A + ++ R Q+
Sbjct: 75 HSPDYTPS---ETSPSPSRSRSASPSSRAAPYGLRSRGGAGENKETENPSGIRSSRSRQH 131
Query: 562 GGATR 576
G R
Sbjct: 132 AGNIR 136
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 33.5 bits (73), Expect = 0.13
Identities = 19/50 (38%), Positives = 32/50 (64%)
Frame = +3
Query: 312 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 461
S +F+RR+ LPE A E V++ + +GVL++T P KV ++ + V I+
Sbjct: 108 SGKFMRRFRLPENAKVEEVKASM-ENGVLSVTVP-KVQESKPEVKSVDIS 155
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 33.1 bits (72), Expect = 0.17
Identities = 15/35 (42%), Positives = 23/35 (65%)
Frame = +3
Query: 312 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPR 416
S QF RR+ LPE + V++ + +GVLT+T P+
Sbjct: 105 SGQFTRRFRLPENVKMDQVKAAM-ENGVLTVTVPK 138
>At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock
protein (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family: identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 134
Score = 30.3 bits (65), Expect = 1.2
Identities = 18/79 (22%), Positives = 33/79 (41%)
Frame = +3
Query: 186 DKFQVNLDVQHFAPEEISVKTADGYIVVXXXXXXXXXXXXYISRQFVRRYALPEGAAPET 365
D ++D+ EEI V+ D ++ + F R++ LPE
Sbjct: 35 DSHTFSVDLPGLRKEEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESIDMIG 94
Query: 366 VESRLSSDGVLTITAPRKV 422
+ + DGVLT+ P+++
Sbjct: 95 ISAGYE-DGVLTVIVPKRI 112
>At1g02840.3 68414.m00246 pre-mRNA splicing factor SF2 (SF2) / SR1
protein identical to SP|O22315 Pre-mRNA splicing factor
SF2 (SR1 protein) {Arabidopsis thaliana}
Length = 303
Score = 30.3 bits (65), Expect = 1.2
Identities = 27/92 (29%), Positives = 42/92 (45%), Gaps = 2/92 (2%)
Frame = +1
Query: 256 GTS*WKASTKRRKTSTGIFQGSSSDVTRCLKARRLRLWNRDCHQTGYSPSLR-RGRCPTP 432
G S K+ ++ R S + S +R KA+ R T SP R + R P+P
Sbjct: 207 GRSYSKSRSRSRGRSVSRSRSRSRSRSRSPKAKSSR--RSPAKSTSRSPGPRSKSRSPSP 264
Query: 433 SRERERCPSHRPVPFARRSRTRA-KKPTRRKS 525
R R R S P+P ++ +++ KP+ KS
Sbjct: 265 RRSRSR--SRSPLPSVQKEGSKSPSKPSPAKS 294
>At1g02840.1 68414.m00245 pre-mRNA splicing factor SF2 (SF2) / SR1
protein identical to SP|O22315 Pre-mRNA splicing factor
SF2 (SR1 protein) {Arabidopsis thaliana}
Length = 303
Score = 30.3 bits (65), Expect = 1.2
Identities = 27/92 (29%), Positives = 42/92 (45%), Gaps = 2/92 (2%)
Frame = +1
Query: 256 GTS*WKASTKRRKTSTGIFQGSSSDVTRCLKARRLRLWNRDCHQTGYSPSLR-RGRCPTP 432
G S K+ ++ R S + S +R KA+ R T SP R + R P+P
Sbjct: 207 GRSYSKSRSRSRGRSVSRSRSRSRSRSRSPKAKSSR--RSPAKSTSRSPGPRSKSRSPSP 264
Query: 433 SRERERCPSHRPVPFARRSRTRA-KKPTRRKS 525
R R R S P+P ++ +++ KP+ KS
Sbjct: 265 RRSRSR--SRSPLPSVQKEGSKSPSKPSPAKS 294
>At3g56100.1 68416.m06235 leucine-rich repeat transmembrane protein
kinase, putative hypothetical proteins - Arabidopsis
thaliana
Length = 719
Score = 29.9 bits (64), Expect = 1.6
Identities = 14/25 (56%), Positives = 16/25 (64%)
Frame = +1
Query: 391 GYSPSLRRGRCPTPSRERERCPSHR 465
GYS S P+PS E+ER PSHR
Sbjct: 342 GYSVSTPCPTLPSPSPEKERKPSHR 366
>At4g14830.1 68417.m02280 expressed protein
Length = 152
Score = 29.1 bits (62), Expect = 2.8
Identities = 13/31 (41%), Positives = 16/31 (51%)
Frame = +3
Query: 324 VRRYALPEGAAPETVESRLSSDGVLTITAPR 416
V R+ LPE PE V DG L +T P+
Sbjct: 109 VWRFRLPESTRPELVTVDCDGDGELIVTVPK 139
>At1g76770.1 68414.m08934 heat shock protein-related contains
similarity to 17.9 kDa heat-shock protein [Helianthus
annuus] gi|11990130|emb|CAB55634
Length = 244
Score = 29.1 bits (62), Expect = 2.8
Identities = 19/73 (26%), Positives = 40/73 (54%), Gaps = 3/73 (4%)
Frame = +3
Query: 315 RQFVRRYALPEGAAPETVESRLSS-DGVLTITAPRKVPDAVKGERKVPIAQTGPVRKE-- 485
++F + + +P+ + +++R + DG LT+T P+KV + G K+ KE
Sbjct: 93 KEFKKVFRIPDIVILDKIKARFNEEDGTLTVTMPKKV-KGITG-LKIEEEDEEEEMKEPI 150
Query: 486 IKDQSEEANEKEK 524
+++++EE E E+
Sbjct: 151 VEEKTEEKTEPEE 163
>At2g34640.1 68415.m04255 expressed protein
Length = 527
Score = 28.7 bits (61), Expect = 3.8
Identities = 17/63 (26%), Positives = 25/63 (39%)
Frame = +1
Query: 310 FQGSSSDVTRCLKARRLRLWNRDCHQTGYSPSLRRGRCPTPSRERERCPSHRPVPFARRS 489
+ S+S R + D G S +R R P P+ E PS+ P +RR
Sbjct: 90 YMDSTSGKLEPASGARASIPGEDYWPEGTSSRVRAARAPQPAGESSSFPSYGKNPGSRRK 149
Query: 490 RTR 498
+ R
Sbjct: 150 KNR 152
>At2g28680.1 68415.m03486 cupin family protein similar to legumin
(11S-globulin) from Ginkgo biloba [GI:949869], 11S
globulin from Avena sativa [GI:472867]; contains a 11-S
plant seed storage protein signature (PS00305)
Length = 356
Score = 28.7 bits (61), Expect = 3.8
Identities = 21/64 (32%), Positives = 35/64 (54%), Gaps = 3/64 (4%)
Frame = +3
Query: 312 SRQFV-RRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERK--VPIAQTGPVRK 482
S +FV R + L E + V S+ + +G++ + A K+P+ KG+RK V P+
Sbjct: 141 STEFVGRAWDLDETTVKKLVGSQ-TGNGIVKVDASLKMPEPKKGDRKGFVLNCLEAPLDV 199
Query: 483 EIKD 494
+IKD
Sbjct: 200 DIKD 203
>At2g20360.1 68415.m02377 expressed protein
Length = 402
Score = 28.3 bits (60), Expect = 5.0
Identities = 13/26 (50%), Positives = 15/26 (57%)
Frame = -1
Query: 476 NGTGLCDGHLSLSLDGVGHLPRRSDG 399
NGT C SL+ GVGHL R+ G
Sbjct: 36 NGTDNCRYSSSLATKGVGHLARKGTG 61
>At1g66720.1 68414.m07584 S-adenosyl-L-methionine:carboxyl
methyltransferase family protein similar to
defense-related protein cjs1 [Brassica
carinata][GI:14009292][Mol Plant Pathol (2001)
2(3):159-169]
Length = 352
Score = 28.3 bits (60), Expect = 5.0
Identities = 10/31 (32%), Positives = 19/31 (61%)
Frame = +1
Query: 322 SSDVTRCLKARRLRLWNRDCHQTGYSPSLRR 414
+S + + + R LWNRD H TG++ +++
Sbjct: 151 TSKIPKGISDRNSPLWNRDMHCTGFNNKVKK 181
>At4g22590.1 68417.m03259 trehalose-6-phosphate phosphatase,
putative similar to trehalose-6-phosphate phosphatase
(AtTPPA) GI:2944178; contains Pfam profile PF02358:
Trehalose-phosphatase
Length = 377
Score = 27.5 bits (58), Expect = 8.7
Identities = 16/57 (28%), Positives = 28/57 (49%)
Frame = +3
Query: 18 SRRPRRLLDQHFGLALTPDDLLSVAAGPLLNREYYRPWRHLAAAARDVGSSIKVDKD 188
S PR+ L + F + + P+D S A L + H+AA A++ ++ +D D
Sbjct: 59 SSPPRKRLVKDFNIEIAPEDDFSQRAWMLKYPSAITSFAHIAAQAKNKKIAVFLDYD 115
>At1g66880.1 68414.m07601 serine/threonine protein kinase family
protein contains protein kinase domain, Pfam:PF00069;
contains serine/threonine protein kinase domain,
INTERPRO:IPR002290
Length = 1296
Score = 27.5 bits (58), Expect = 8.7
Identities = 13/38 (34%), Positives = 17/38 (44%)
Frame = -2
Query: 118 YSLFSKGPAATLRRSSGVKARPKCWSNRRRGRLEPKSS 5
Y S G A + +ARP CWS R +E S+
Sbjct: 1041 YEYISNGTLAEHLHGNRAEARPLCWSTRLNIAIETASA 1078
>At1g60650.2 68414.m06828 glycine-rich RNA-binding protein, putative
similar to RNA binding protein(RZ-1) GI:1435061 from
[Nicotiana sylvestris]; contains InterPro entry
IPR000504: RNA-binding region RNP-1 (RNA recognition
motif) (RRM)
Length = 292
Score = 27.5 bits (58), Expect = 8.7
Identities = 15/35 (42%), Positives = 15/35 (42%)
Frame = +1
Query: 340 CLKARRLRLWNRDCHQTGYSPSLRRGRCPTPSRER 444
C K RR W RDC TG R R P R R
Sbjct: 117 CFKCRRPGHWARDCPSTG--DDRERFRVPLAMRSR 149
>At1g60650.1 68414.m06827 glycine-rich RNA-binding protein, putative
similar to RNA binding protein(RZ-1) GI:1435061 from
[Nicotiana sylvestris]; contains InterPro entry
IPR000504: RNA-binding region RNP-1 (RNA recognition
motif) (RRM)
Length = 292
Score = 27.5 bits (58), Expect = 8.7
Identities = 15/35 (42%), Positives = 15/35 (42%)
Frame = +1
Query: 340 CLKARRLRLWNRDCHQTGYSPSLRRGRCPTPSRER 444
C K RR W RDC TG R R P R R
Sbjct: 117 CFKCRRPGHWARDCPSTG--DDRERFRVPLAMRSR 149
>At1g05830.1 68414.m00610 trithorax protein, putative / PHD finger
family protein / SET domain-containing protein similar
to trithorax-like protein 1 [Arabidopsis thaliana]
GI:12659210; contains Pfam domain, PF00628: PHD-finger
and PF00856: SET domain
Length = 1056
Score = 27.5 bits (58), Expect = 8.7
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +1
Query: 502 KKPTRRKSRKVYEELRLLQNGGATRD 579
+K RR+ R+ + EL +LQN G RD
Sbjct: 89 RKRRRRRRRESFLELAILQNEGVERD 114
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,123,141
Number of Sequences: 28952
Number of extensions: 246439
Number of successful extensions: 820
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 782
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 810
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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