Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0908
         (705 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1751.03 ||SPAC31A2.01|translation initiation factor eIF3m|Sc...    43   5e-05
SPAC25G10.08 |||translation initiation factor eIF3b |Schizosacch...    29   0.86 
SPBC337.03 |||conserved eukaryotic protein|Schizosaccharomyces p...    29   0.86 
SPAC6F6.08c |cdc16|bub2|two-component GAP Cdc16|Schizosaccharomy...    27   3.5  
SPAC27F1.04c |nuf2||spindle pole body protein Nuf2|Schizosacchar...    26   6.0  
SPBC1289.06c |||sequence orphan|Schizosaccharomyces pombe|chr 2|...    26   6.0  
SPCC4G3.19 |alp16||gamma tubulin complex subunit Alp16 |Schizosa...    25   8.0  
SPCC1919.01 |ppk34|SPCC830.12|serine/threonine protein kinase Pp...    25   8.0  

>SPAC1751.03 ||SPAC31A2.01|translation initiation factor
           eIF3m|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 402

 Score = 42.7 bits (96), Expect = 5e-05
 Identities = 37/148 (25%), Positives = 72/148 (48%), Gaps = 9/148 (6%)
 Frame = +1

Query: 172 LEQFVLLAKGAKGSACAELIKQVLEAPGVHVFGELLEMPNIKELESGPYATHLKTLNLFA 351
           L + V +       A  EL+++ + +P    F +++ +P +++LE       L+ L + +
Sbjct: 196 LLEAVKMDNSTADEAVRELVQRAVNSPKYFFFDDIVTLPPVQQLEQST----LQLLGILS 251

Query: 352 YGTYKDYI----ENKSDYLELTTVQ---CKKLQHLTIATLATQ--EKCIPYSVLLEELDI 504
            G   DY+    EN +        +    +K++ LTIA+LATQ     + Y  + + L I
Sbjct: 252 GGMTDDYVSWVAENHAHCQHQKFDEDAIARKMKLLTIASLATQAPNNTLSYGDVAKSLKI 311

Query: 505 KNVRDLEDLIIEAIYADIIHGKLDQECK 588
            +  ++E  II+ I A ++ G++ Q  K
Sbjct: 312 -DENEVELWIIDVIRAGLVEGRMSQLTK 338


>SPAC25G10.08 |||translation initiation factor eIF3b
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 725

 Score = 28.7 bits (61), Expect = 0.86
 Identities = 25/84 (29%), Positives = 40/84 (47%), Gaps = 3/84 (3%)
 Frame = +1

Query: 271 ELLEMPNIKELESGPYATHLKTLNLFAYGTYKDYIENKSDYLELTTVQCKKLQHLTIATL 450
           E+   P    L S P  + ++T NLF     K Y ++  DYL +   +  K +  T + L
Sbjct: 379 EITNQPARVALISIPSKSTIRTKNLFNVSDCKLYWQSNGDYLCVKVDRHTKTKKSTFSNL 438

Query: 451 ---ATQEKCIPYSVLLEELDIKNV 513
                +EK IP    +E +D+K+V
Sbjct: 439 EIFRIREKNIP----VEVVDLKDV 458


>SPBC337.03 |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 387

 Score = 28.7 bits (61), Expect = 0.86
 Identities = 18/63 (28%), Positives = 27/63 (42%), Gaps = 1/63 (1%)
 Frame = +1

Query: 316 YATHLKTLNLFAYGTYKDYIENKSDYLELTTVQCKKLQHLTIATLATQEKCI-PYSVLLE 492
           YA    +        YK Y+E +SDY EL     K+L   + +     + CI   S L+ 
Sbjct: 161 YAKSAHSTKTVVDALYKSYLEKQSDYSELLDNYKKQLNKASESCKGNYQACIESRSTLIT 220

Query: 493 ELD 501
            L+
Sbjct: 221 SLE 223


>SPAC6F6.08c |cdc16|bub2|two-component GAP Cdc16|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 299

 Score = 26.6 bits (56), Expect = 3.5
 Identities = 12/37 (32%), Positives = 22/37 (59%), Gaps = 3/37 (8%)
 Frame = +1

Query: 400 LTTVQCKKLQHLTIATLATQEKCIP---YSVLLEELD 501
           + ++ CK+LQ L   +   Q KCI    Y V+L++++
Sbjct: 1   MPSIDCKRLQKLAPKSPENQSKCISRLRYMVMLDQVE 37


>SPAC27F1.04c |nuf2||spindle pole body protein
           Nuf2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 441

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 13/46 (28%), Positives = 23/46 (50%)
 Frame = +1

Query: 394 LELTTVQCKKLQHLTIATLATQEKCIPYSVLLEELDIKNVRDLEDL 531
           LE   V+  KL+H T+     QE C    +  ++L+ +  + L+ L
Sbjct: 295 LEECLVELDKLEHATVLLSTNQELCDQIEINKKKLEFRKEQLLKQL 340


>SPBC1289.06c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 481

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 12/32 (37%), Positives = 20/32 (62%)
 Frame = +1

Query: 505 KNVRDLEDLIIEAIYADIIHGKLDQECKRVGS 600
           K+ R L +L I A+ A I+ G   ++C ++GS
Sbjct: 173 KDERVLGNLYISALQAKILGGASFEQCGKIGS 204


>SPCC4G3.19 |alp16||gamma tubulin complex subunit Alp16
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 759

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 11/27 (40%), Positives = 19/27 (70%)
 Frame = -1

Query: 378 LNVVLICAIGKQIKSF*MSCIRSTLQL 298
           LN+ + C I + I+SF +S +RST ++
Sbjct: 491 LNLNVCCCIAEPIQSFILSFLRSTYKV 517


>SPCC1919.01 |ppk34|SPCC830.12|serine/threonine protein kinase
           Ppk34|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 354

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 11/29 (37%), Positives = 18/29 (62%)
 Frame = +1

Query: 448 LATQEKCIPYSVLLEELDIKNVRDLEDLI 534
           +  QEK  PY  + +ELD+  V D E+++
Sbjct: 106 IRNQEKKDPYYYIRKELDVLKVLDHENVV 134


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,933,092
Number of Sequences: 5004
Number of extensions: 58366
Number of successful extensions: 152
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 327172622
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)