Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0898
(710 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual 50 2e-07
SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr 1|||... 38 0.001
SPBC336.05c |||S-adenosylmethionine-dependentmethyltransferase|S... 33 0.053
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 28 1.5
SPAC17H9.01 |cid16||poly|Schizosaccharomyces pombe|chr 1|||Manual 27 2.0
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 27 2.6
SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosa... 26 6.1
SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |... 26 6.1
SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase Met... 25 8.1
>SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual
Length = 272
Score = 50.4 bits (115), Expect = 2e-07
Identities = 28/87 (32%), Positives = 49/87 (56%)
Frame = +2
Query: 368 NIICFQELWNMPFAFCTREKQPWCEFAESDEDGPTTTFLRELAIKYAMVIVSSILERDEK 547
N+I F EL + C + + AE +GP+ + LA KY + I+ E++EK
Sbjct: 39 NLILFPELITSGYE-CGNT---FTQIAEIAGEGPSFKTMSNLAAKYHVNIIYGFPEKEEK 94
Query: 548 HSDILWNTAVVISDTGNVIGKHRKNHI 628
S+I++N+ + I++ GN+ G +RK H+
Sbjct: 95 QSNIIYNSCIYITENGNLGGVYRKVHL 121
>SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 322
Score = 37.9 bits (84), Expect = 0.001
Identities = 35/130 (26%), Positives = 60/130 (46%)
Frame = +2
Query: 239 RIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCT 418
R ++G+VQ +A D+ N Q + K+++ A + G N+I E++N P+ T
Sbjct: 42 RAFRIGLVQ--LANTKDKSENLQLARL-----KVLEAA-KNGSNVIVLPEIFNSPYG--T 91
Query: 419 REKQPWCEFAESDEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGN 598
+ E E E P+ L +A + + E+ L+NTA+V +G
Sbjct: 92 GYFNQYAEPIE--ESSPSYQALSSMAKDTKTYLFGGSIP--ERKDGKLYNTAMVFDPSGK 147
Query: 599 VIGKHRKNHI 628
+I HRK H+
Sbjct: 148 LIAVHRKIHL 157
>SPBC336.05c |||S-adenosylmethionine-
dependentmethyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 378
Score = 32.7 bits (71), Expect = 0.053
Identities = 21/64 (32%), Positives = 31/64 (48%)
Frame = -3
Query: 480 KVVVGPSSSDSANSHHGCFSLVQNAKGMFHNSWKQMMLTPSWPATSMIFLTLLKIAFFCS 301
++ V S ++A SH CF QN+ + + + +P T F+ LLK AFF
Sbjct: 232 QIAVFHQSKNNAASH--CFLKDQNSSILLYKKITYPFMEQLFPPTVQQFMNLLKKAFFDH 289
Query: 300 LTGR 289
L GR
Sbjct: 290 LFGR 293
>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1610
Score = 27.9 bits (59), Expect = 1.5
Identities = 9/18 (50%), Positives = 16/18 (88%)
Frame = +2
Query: 509 MVIVSSILERDEKHSDIL 562
+++VS++LE DEKH D++
Sbjct: 998 LIVVSNLLEMDEKHVDVV 1015
>SPAC17H9.01 |cid16||poly|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1202
Score = 27.5 bits (58), Expect = 2.0
Identities = 29/116 (25%), Positives = 48/116 (41%)
Frame = -2
Query: 433 RLLLPCAEREGHVPQLLETDDVNTLLAGNIDDFLDFIENCFLLLVDWTIGGHRDGMLNYS 254
R LL C +R P +L DV ++DD + F+ T GH++ + N S
Sbjct: 119 RALLSCCKRSKD-PSILFPTDVPC----SLDDDVSFL----------TFKGHKNHLENRS 163
Query: 253 YLHNSRGSGLLVLGRESVCGDVEVSLLSCSDRGFFQFDFKVIPPPKMNSVELFQVA 86
+ H+S V+ E + L DR K++ P N ++L +V+
Sbjct: 164 FFHDSESDNFKVVLSNCAINSKEDNNLVTEDR--VNLGAKLLLVPVQNLIKLLKVS 217
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 27.1 bits (57), Expect = 2.6
Identities = 17/52 (32%), Positives = 28/52 (53%)
Frame = -2
Query: 331 DFIENCFLLLVDWTIGGHRDGMLNYSYLHNSRGSGLLVLGRESVCGDVEVSL 176
+FI+ CF + + GH D +++ S +S GS +G S D++VSL
Sbjct: 661 EFIQRCFHFADEASPDGHSDTLIDISDHMSSTGSENRSVGANS---DIKVSL 709
>SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase
Ubp10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 502
Score = 25.8 bits (54), Expect = 6.1
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +2
Query: 71 NNLTGRDLEEFNRIHFGRRNNLEI 142
NN+ R +EE N I G+R LE+
Sbjct: 8 NNILKRHIEEDNNIDNGKRKKLEL 31
>SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 499
Score = 25.8 bits (54), Expect = 6.1
Identities = 13/38 (34%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = +2
Query: 41 ETHSLESII-NNNLTGRDLEEFNRIHFGRRNNLEIKLK 151
E H +E +I + N+T DL F + FG+ N ++ K
Sbjct: 369 EFHQVEGVICDRNITLGDLIGFLEVFFGKMNVKNLRFK 406
>SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase
Met11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 641
Score = 25.4 bits (53), Expect = 8.1
Identities = 14/43 (32%), Positives = 22/43 (51%)
Frame = -2
Query: 331 DFIENCFLLLVDWTIGGHRDGMLNYSYLHNSRGSGLLVLGRES 203
D N F+ ++ GH+D + + S GSG VLGR++
Sbjct: 353 DKSSNLFVTSKQSSVSGHKDNLTEEAPFSVSEGSG--VLGRQA 393
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,030,136
Number of Sequences: 5004
Number of extensions: 63415
Number of successful extensions: 219
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 204
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 218
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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