Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0898
(710 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 230 6e-61
At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei... 54 1e-07
At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei... 54 1e-07
At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei... 50 1e-06
At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei... 49 3e-06
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460... 33 0.19
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329... 33 0.25
At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P460... 31 0.57
At5g67240.1 68418.m08475 exonuclease family protein contains exo... 29 3.0
At5g40740.1 68418.m04944 expressed protein 29 3.0
At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilas... 29 3.0
At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329... 29 3.0
At5g02880.1 68418.m00231 HECT-domain-containing protein / ubiqui... 29 4.0
At4g13750.1 68417.m02134 expressed protein 29 4.0
At5g06970.1 68418.m00789 expressed protein 28 7.0
At2g39980.1 68415.m04913 transferase family protein contains Pfa... 28 7.0
At4g00700.1 68417.m00096 C2 domain-containing protein contains I... 27 9.3
At2g33010.1 68415.m04045 ubiquitin-associated (UBA)/TS-N domain-... 27 9.3
At2g30650.1 68415.m03738 3-hydroxyisobutyryl-coenzyme A hydrolas... 27 9.3
At1g28610.2 68414.m03523 GDSL-motif lipase, putative similar to ... 27 9.3
At1g28610.1 68414.m03524 GDSL-motif lipase, putative similar to ... 27 9.3
>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
beta-alanine synthase, putative similar to beta-alanine
synthase [Dictyostelium discoideum] GI:14334061;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 408
Score = 230 bits (563), Expect = 6e-61
Identities = 115/209 (55%), Positives = 144/209 (68%), Gaps = 3/209 (1%)
Frame = +2
Query: 50 SLESIINNNLTGRDLEEFNRIHFGR---RNNLEIKLKESSIXXXXXXXXXXXXXXFPAKD 220
SL +++ NL +E NR+ GR R+ +I L ES+ F A
Sbjct: 22 SLHQLLSANLKPELYQEVNRLLLGRNCGRSLEQIVLPESAKALSSKHDFDLQAASFSADK 81
Query: 221 EQTRPPRIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNM 400
EQ R PR+V+VG++Q+SIA+PT P ++Q + IF+K+K IID AG GVNI+C QE W M
Sbjct: 82 EQMRNPRVVRVGLIQNSIALPTTAPFSDQTRGIFDKLKPIIDAAGVAGVNILCLQEAWTM 141
Query: 401 PFAFCTREKQPWCEFAESDEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVV 580
PFAFCTRE++ WCEFAE DG +T FL+ELA KY MVIVS ILERD H ++LWNTAV+
Sbjct: 142 PFAFCTRERR-WCEFAEP-VDGESTKFLQELAKKYNMVIVSPILERDIDHGEVLWNTAVI 199
Query: 581 ISDTGNVIGKHRKNHIPRVGDFNESNYYM 667
I + GN+IGKHRKNHIPRVGDFNES YYM
Sbjct: 200 IGNNGNIIGKHRKNHIPRVGDFNESTYYM 228
Score = 27.5 bits (58), Expect = 9.3
Identities = 10/14 (71%), Positives = 12/14 (85%)
Frame = +3
Query: 669 EGNTGHPVFATRYG 710
EG+TGHPVF T +G
Sbjct: 229 EGDTGHPVFETVFG 242
>At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 294
Score = 53.6 bits (123), Expect = 1e-07
Identities = 32/113 (28%), Positives = 55/113 (48%), Gaps = 2/113 (1%)
Frame = +2
Query: 296 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESDED-GP 469
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 470 TTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHI 628
+T L E++ + + I+ + E+ D L+NT V G + KHRK H+
Sbjct: 154 STAMLSEVSKRLKITIIGGSI--PERVGDRLYNTCCVFGSDGELKAKHRKIHL 204
>At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 369
Score = 53.6 bits (123), Expect = 1e-07
Identities = 32/113 (28%), Positives = 55/113 (48%), Gaps = 2/113 (1%)
Frame = +2
Query: 296 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESDED-GP 469
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 470 TTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHI 628
+T L E++ + + I+ + E+ D L+NT V G + KHRK H+
Sbjct: 154 STAMLSEVSKRLKITIIGGSI--PERVGDRLYNTCCVFGSDGELKAKHRKIHL 204
>At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 299
Score = 50.4 bits (115), Expect = 1e-06
Identities = 32/112 (28%), Positives = 61/112 (54%), Gaps = 1/112 (0%)
Frame = +2
Query: 332 KKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESDEDGPTTTFLRELAIKYAM 511
++++ A +G NII QEL+ + FC +++ + + A+ ++ PT +++LA + +
Sbjct: 31 ERLVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGV 89
Query: 512 VI-VSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYY 664
VI VS E + H +N+ +I G +G +RK+HIP + E Y+
Sbjct: 90 VIPVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYF 137
>At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 326
Score = 49.2 bits (112), Expect = 3e-06
Identities = 32/110 (29%), Positives = 59/110 (53%), Gaps = 1/110 (0%)
Frame = +2
Query: 338 IIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESDEDGPTTTFLRELAIKYAMVI 517
++ A +G NII QEL+ + FC +++ + + A+ ++ PT +++LA + +VI
Sbjct: 60 LVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGVVI 118
Query: 518 -VSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYY 664
VS E + H +N+ +I G +G +RK+HIP + E Y+
Sbjct: 119 PVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYF 164
>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 33.1 bits (72), Expect = 0.19
Identities = 41/142 (28%), Positives = 57/142 (40%), Gaps = 13/142 (9%)
Frame = +2
Query: 233 PPRIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELW------ 394
P V+V IVQ S V D P A +K +K I A +G ++ F E +
Sbjct: 21 PSSTVRVTIVQSS-TVYNDTP------ATLDKAEKFIVEAASKGAKLVLFPEAFIGGYPR 73
Query: 395 --NMPFAFCTREKQPWCEFAESDED-----GPTTTFLRELAIKYAMVIVSSILERDEKHS 553
A ++ EF GP L ELA K + +V +E+D
Sbjct: 74 GFRFGLAVGVHNEEGRDEFRNYHASAIKVPGPEVERLAELAGKNNVHLVMGAIEKD---G 130
Query: 554 DILWNTAVVISDTGNVIGKHRK 619
L+ TA+ S G +GKHRK
Sbjct: 131 YTLYCTALFFSPQGQFLGKHRK 152
>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 339
Score = 32.7 bits (71), Expect = 0.25
Identities = 20/52 (38%), Positives = 27/52 (51%)
Frame = +2
Query: 464 GPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 619
GP L ELA K + +V +E+D L+ TA+ S G +GKHRK
Sbjct: 97 GPEVEKLAELAGKNNVYLVMGAIEKD---GYTLYCTALFFSPQGQFLGKHRK 145
>At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P46011
Nitrilase 4 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 355
Score = 31.5 bits (68), Expect = 0.57
Identities = 18/55 (32%), Positives = 27/55 (49%)
Frame = +2
Query: 455 DEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 619
D GP L +A KY + +V ++ER+ L+ T + G +GKHRK
Sbjct: 112 DVPGPEVERLALMAKKYKVYLVMGVIERE---GYTLYCTVLFFDSQGLFLGKHRK 163
>At5g67240.1 68418.m08475 exonuclease family protein contains
exonuclease domain, Pfam:PF00929
Length = 745
Score = 29.1 bits (62), Expect = 3.0
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +1
Query: 472 HDLPSGTRHQVRNGDRVLDIRKGREAFGH 558
HD+PS H V NGD LD++ + G+
Sbjct: 441 HDVPSQELHGVLNGDFTLDVKPPKRKGGY 469
>At5g40740.1 68418.m04944 expressed protein
Length = 741
Score = 29.1 bits (62), Expect = 3.0
Identities = 15/46 (32%), Positives = 23/46 (50%)
Frame = +2
Query: 533 ERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMG 670
ER+ + ++ +++ NVIG N PRVG F SN +G
Sbjct: 8 ERELELESAMYTNCLLLGLDPNVIGLGASNGTPRVGLFRHSNPKLG 53
>At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilase 1
[Mus musculus] GI:3228668; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 307
Score = 29.1 bits (62), Expect = 3.0
Identities = 30/105 (28%), Positives = 46/105 (43%), Gaps = 2/105 (1%)
Frame = +2
Query: 320 FNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESDEDGPTTTFLRELAI 499
F +++ A G +ICF E F+F ++ + AE DGP LA
Sbjct: 54 FATCSRLVQEAALAGAKLICFPE----NFSFVGDKEGESVKIAEP-LDGPVMERYCSLAR 108
Query: 500 KYAMVI-VSSILER-DEKHSDILWNTAVVISDTGNVIGKHRKNHI 628
+ + + ER D+ H L NT VVI D G + ++K H+
Sbjct: 109 DSNIWLSLGGFQERFDDTH---LCNTHVVIDDAGMIRDTYQKMHL 150
>At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 29.1 bits (62), Expect = 3.0
Identities = 39/142 (27%), Positives = 57/142 (40%), Gaps = 13/142 (9%)
Frame = +2
Query: 233 PPRIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQE--LWNMPF 406
P V+V IVQ S V D P A +K +K I A +G ++ F E + P
Sbjct: 21 PSTTVRVTIVQSS-TVYNDTP------ATIDKAEKYIVEAASKGAELVLFPEGFIGGYPR 73
Query: 407 AFC------TREKQPWCEFAESDED-----GPTTTFLRELAIKYAMVIVSSILERDEKHS 553
F ++ EF + GP L ++A K + +V +E K
Sbjct: 74 GFRFGLAVGVHNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIE---KEG 130
Query: 554 DILWNTAVVISDTGNVIGKHRK 619
L+ T + S G +GKHRK
Sbjct: 131 YTLYCTVLFFSPQGQFLGKHRK 152
>At5g02880.1 68418.m00231 HECT-domain-containing protein /
ubiquitin-transferase family protein /
armadillo/beta-catenin-like repeat-containing protein
similar to SP|Q14669 Thyroid receptor interacting
protein 12 (TRIP12) {Homo sapiens}; contains Pfam
profiles PF00632: HECT-domain (ubiquitin-transferase),
PF00514: Armadillo/beta-catenin-like repeat
Length = 1502
Score = 28.7 bits (61), Expect = 4.0
Identities = 17/71 (23%), Positives = 36/71 (50%)
Frame = -2
Query: 472 GRPVFI*FGKLAPRLLLPCAEREGHVPQLLETDDVNTLLAGNIDDFLDFIENCFLLLVDW 293
G V++ +G L+ L C + G + +LL+ +++++LAG + + L + +
Sbjct: 454 GANVYVSYGCLSAIHKLTCLSKSGDIVELLKNTNMSSVLAGILSRKDHHVIVVALQVAEV 513
Query: 292 TIGGHRDGMLN 260
+ +RD LN
Sbjct: 514 LLEKYRDTFLN 524
>At4g13750.1 68417.m02134 expressed protein
Length = 2137
Score = 28.7 bits (61), Expect = 4.0
Identities = 14/72 (19%), Positives = 37/72 (51%), Gaps = 2/72 (2%)
Frame = +2
Query: 371 IICFQELWNMPFAFCTREKQPWCEFAESDEDGPTTTFLRELAIKYAMVI--VSSILERDE 544
++C+ + + F ++K + F E+D++G + + +++ I +S +++R+
Sbjct: 1660 LVCWCDNEKLKKRFKNKDKIEFISFGENDDEGQEVLQTKVSGLMHSLGIPSISEVVKREA 1719
Query: 545 KHSDILWNTAVV 580
K+ + NT V
Sbjct: 1720 KYEGLQDNTVTV 1731
>At5g06970.1 68418.m00789 expressed protein
Length = 1101
Score = 27.9 bits (59), Expect = 7.0
Identities = 13/59 (22%), Positives = 27/59 (45%)
Frame = -3
Query: 615 RCFPITFPVSLITTAVFQSMSECFSSLSNIEDTITIAYLMASSRRKVVVGPSSSDSANS 439
R I P + + ++ S LS +ED++ + ++ R K+V+ S + + S
Sbjct: 834 RSINIDVPATAMLCVQLNTLHYAVSQLSKLEDSMWLRWIAKKPREKIVIRKSMVEKSKS 892
>At2g39980.1 68415.m04913 transferase family protein contains Pfam
profile PF02458 transferase family
Length = 482
Score = 27.9 bits (59), Expect = 7.0
Identities = 12/28 (42%), Positives = 14/28 (50%)
Frame = -1
Query: 590 CH*LQPQCSKVCPNASRPFLISRTRSPL 507
CH +Q C CPN P LIS + L
Sbjct: 39 CHYIQKGCLFTCPNLPLPALISHLKHSL 66
>At4g00700.1 68417.m00096 C2 domain-containing protein contains
INTERPRO:IPR000008 C2 domain
Length = 1006
Score = 27.5 bits (58), Expect = 9.3
Identities = 19/63 (30%), Positives = 28/63 (44%)
Frame = +2
Query: 449 ESDEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHI 628
++DE TF L + I+S+ L HS LW V I + +VI K+ +
Sbjct: 394 QADEAFGDATFSDSLVDSDSSNIISANLRSKVYHSPRLWYLRVQILEAQDVIIVSDKSRV 453
Query: 629 PRV 637
P V
Sbjct: 454 PEV 456
>At2g33010.1 68415.m04045 ubiquitin-associated (UBA)/TS-N
domain-containing protein contains Pfam profile PF00627:
UBA/TS-N domain
Length = 649
Score = 27.5 bits (58), Expect = 9.3
Identities = 17/38 (44%), Positives = 25/38 (65%), Gaps = 2/38 (5%)
Frame = -3
Query: 591 VSLITTAVFQSMSECFSSLSNIEDT-ITIAYL-MASSR 484
+ L + + FQ+ FSSL+NI+ T T+A L MASS+
Sbjct: 482 IGLPSQSQFQASGSSFSSLTNIDPTDFTVANLPMASSQ 519
>At2g30650.1 68415.m03738 3-hydroxyisobutyryl-coenzyme A hydrolase,
putative / CoA-thioester hydrolase, putative strong
similarity to gi:8572760; contains Pfam profile PF00388
enoyl-CoA hydratase/isomerase family protein
Length = 422
Score = 27.5 bits (58), Expect = 9.3
Identities = 14/43 (32%), Positives = 19/43 (44%)
Frame = -1
Query: 638 RLSECGSCDVSRSRFRCH*LQPQCSKVCPNASRPFLISRTRSP 510
RL G C + R CH ++ SK R LI + R+P
Sbjct: 332 RLQGVGQCLIREYRMVCHVMKGDISKDFVEGCRAVLIDKDRNP 374
>At1g28610.2 68414.m03523 GDSL-motif lipase, putative similar to
lipase [Arabidopsis thaliana] GI:1145627; contains Pfam
profile: PF00657 Lipase/Acylhydrolase with GDSL-like
motif
Length = 383
Score = 27.5 bits (58), Expect = 9.3
Identities = 9/17 (52%), Positives = 14/17 (82%)
Frame = +2
Query: 581 ISDTGNVIGKHRKNHIP 631
I+DTGN++G +NH+P
Sbjct: 39 ITDTGNLVGLSDRNHLP 55
>At1g28610.1 68414.m03524 GDSL-motif lipase, putative similar to
lipase [Arabidopsis thaliana] GI:1145627; contains Pfam
profile: PF00657 Lipase/Acylhydrolase with GDSL-like
motif
Length = 250
Score = 27.5 bits (58), Expect = 9.3
Identities = 9/17 (52%), Positives = 14/17 (82%)
Frame = +2
Query: 581 ISDTGNVIGKHRKNHIP 631
I+DTGN++G +NH+P
Sbjct: 39 ITDTGNLVGLSDRNHLP 55
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,239,522
Number of Sequences: 28952
Number of extensions: 350230
Number of successful extensions: 1098
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 1057
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1094
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1535986264
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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