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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0880
         (648 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_57342| Best HMM Match : Arf (HMM E-Value=0)                         33   0.15 
SB_50497| Best HMM Match : CH (HMM E-Value=0.0084)                     32   0.35 
SB_15028| Best HMM Match : Drf_FH1 (HMM E-Value=0.84)                  32   0.35 
SB_2435| Best HMM Match : WD40 (HMM E-Value=4.1e-10)                   31   1.1  
SB_53660| Best HMM Match : COLFI (HMM E-Value=1.5)                     30   1.4  
SB_3888| Best HMM Match : No HMM Matches (HMM E-Value=.)               30   1.4  
SB_19938| Best HMM Match : TolA (HMM E-Value=0.22)                     29   2.5  
SB_57077| Best HMM Match : RhoGEF (HMM E-Value=2.1e-07)                29   2.5  
SB_1955| Best HMM Match : No HMM Matches (HMM E-Value=.)               29   2.5  
SB_50167| Best HMM Match : PRP1_N (HMM E-Value=0.87)                   29   3.3  
SB_2672| Best HMM Match : GSHPx (HMM E-Value=4.1e-14)                  29   3.3  
SB_13605| Best HMM Match : MscL (HMM E-Value=6.60012e-43)              29   4.3  
SB_54212| Best HMM Match : No HMM Matches (HMM E-Value=.)              27   9.9  
SB_25209| Best HMM Match : Astacin (HMM E-Value=7.69999e-41)           27   9.9  
SB_971| Best HMM Match : No HMM Matches (HMM E-Value=.)                27   9.9  
SB_55819| Best HMM Match : No HMM Matches (HMM E-Value=.)              27   9.9  
SB_29493| Best HMM Match : PH (HMM E-Value=0.48)                       27   9.9  
SB_23980| Best HMM Match : Mur_ligase_C (HMM E-Value=0.63)             27   9.9  

>SB_57342| Best HMM Match : Arf (HMM E-Value=0)
          Length = 457

 Score = 33.5 bits (73), Expect = 0.15
 Identities = 18/52 (34%), Positives = 29/52 (55%), Gaps = 2/52 (3%)
 Frame = +3

Query: 321 FPIEREV--ILSSGEKNSKVPILQEARKRGRLGAVAPDTPPKTLSAWTHSEN 470
           F +  E+  +L   E N  VP++  A K+  LGA+ PD   + LS + H++N
Sbjct: 194 FEVREELTRVLQHSELNG-VPVVVVANKQDLLGAIGPDKMAEELSLYKHTKN 244


>SB_50497| Best HMM Match : CH (HMM E-Value=0.0084)
          Length = 2086

 Score = 32.3 bits (70), Expect = 0.35
 Identities = 17/53 (32%), Positives = 25/53 (47%)
 Frame = +2

Query: 278  SASRTCRPAHTLRKISYRTRSDIIFRRKEFQSANTSRGSKTRQTRRRGSRHPT 436
            SA  T RPA T +K++  +       +K+ + A T+  SKTR         PT
Sbjct: 1782 SAKTTTRPASTTKKLATSSTKPATDDKKQTKPATTTNASKTRPASSAAKPKPT 1834


>SB_15028| Best HMM Match : Drf_FH1 (HMM E-Value=0.84)
          Length = 944

 Score = 32.3 bits (70), Expect = 0.35
 Identities = 17/53 (32%), Positives = 25/53 (47%)
 Frame = +2

Query: 278 SASRTCRPAHTLRKISYRTRSDIIFRRKEFQSANTSRGSKTRQTRRRGSRHPT 436
           SA  T RPA T +K++  +       +K+ + A T+  SKTR         PT
Sbjct: 640 SAKTTTRPASTTKKLATSSTKPATDDKKQTKPATTTNASKTRPASSAAKPKPT 692


>SB_2435| Best HMM Match : WD40 (HMM E-Value=4.1e-10)
          Length = 1272

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 18/71 (25%), Positives = 35/71 (49%)
 Frame = +2

Query: 311 LRKISYRTRSDIIFRRKEFQSANTSRGSKTRQTRRRGSRHPTKNTLSLDSLGEPSRHIGR 490
           L + ++  + D++   +E +    +   +    +R  S    +NT+ L  L  PSR + R
Sbjct: 648 LEEEAFYRQQDLVMSAEEQRRKMIAEEERKLTDQRVRSVTEQQNTVKLIQLAGPSRFVLR 707

Query: 491 LQTIPRELQLQ 523
           LQ + RE+ L+
Sbjct: 708 LQAMKREVHLR 718


>SB_53660| Best HMM Match : COLFI (HMM E-Value=1.5)
          Length = 471

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 13/50 (26%), Positives = 22/50 (44%)
 Frame = +3

Query: 423 PDTPPKTLSAWTHSENQAGTSADYRQYHENYNYNMQQNQSGTRNGNYDSP 572
           PD PP+ L  WT      G   D+    +N+  N   N + + +   ++P
Sbjct: 260 PDVPPRALGGWTLVARINGAGTDFSPISDNWANNETFNANTSTDITMETP 309


>SB_3888| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 986

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 15/65 (23%), Positives = 31/65 (47%)
 Frame = +2

Query: 353 RRKEFQSANTSRGSKTRQTRRRGSRHPTKNTLSLDSLGEPSRHIGRLQTIPRELQLQHAA 532
           +R+   S+    GSK+++ R+     P K T   D+     +H    +T  R + +++  
Sbjct: 81  KRQNSVSSTAQNGSKSKRKRKHSELDPVKYTAEEDAKENKRKHKKSQKTSKRVVTMENLV 140

Query: 533 ESEWY 547
           E++ Y
Sbjct: 141 ENKDY 145


>SB_19938| Best HMM Match : TolA (HMM E-Value=0.22)
          Length = 2279

 Score = 29.5 bits (63), Expect = 2.5
 Identities = 22/88 (25%), Positives = 33/88 (37%), Gaps = 1/88 (1%)
 Frame = +3

Query: 309  HSAKFPIEREVILSSGEKNSKVPILQEARKRGRLGAVA-PDTPPKTLSAWTHSENQAGTS 485
            H ++  IE E   +   K+     +   RK    G +  P TP  T  +   +  Q G S
Sbjct: 1714 HHSQSKIEAEKRSTKKGKHLNNHEVDYPRKGHACGVMGRPSTPSSTKRSSYATATQKGNS 1773

Query: 486  ADYRQYHENYNYNMQQNQSGTRNGNYDS 569
                 + E+Y  N         N +YDS
Sbjct: 1774 IGRENHRESYQTNGMVQDGARSNNSYDS 1801


>SB_57077| Best HMM Match : RhoGEF (HMM E-Value=2.1e-07)
          Length = 658

 Score = 29.5 bits (63), Expect = 2.5
 Identities = 16/35 (45%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
 Frame = +3

Query: 321 FPIEREVILSSGEKNSKVPILQEARKRG-RLGAVA 422
           FP ER +  ++GEK   V  ++EA KRG R G+ A
Sbjct: 507 FPCERYLYPTAGEKREMVRSIKEAIKRGIRAGSTA 541


>SB_1955| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 292

 Score = 29.5 bits (63), Expect = 2.5
 Identities = 18/64 (28%), Positives = 30/64 (46%), Gaps = 1/64 (1%)
 Frame = +2

Query: 323 SYRTRSDIIFRRKEFQSANT-SRGSKTRQTRRRGSRHPTKNTLSLDSLGEPSRHIGRLQT 499
           S  + S  I R++E    NT ++G K  + ++          +  DSL  PS+ IG  + 
Sbjct: 76  SLSSPSKKIDRKREVDQENTETKGLKLNKVKQIKDSEKCLEAIDYDSLSSPSKKIGNKRK 135

Query: 500 IPRE 511
           I +E
Sbjct: 136 IDQE 139


>SB_50167| Best HMM Match : PRP1_N (HMM E-Value=0.87)
          Length = 267

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 18/67 (26%), Positives = 31/67 (46%), Gaps = 1/67 (1%)
 Frame = +2

Query: 314 RKISYRTRSDIIFRRKEFQSANT-SRGSKTRQTRRRGSRHPTKNTLSLDSLGEPSRHIGR 490
           +K S  + S  I R+++    NT ++G K  +  +          +  DSL  PS+ IG 
Sbjct: 34  KKDSLSSPSKKIDRKRDVDQENTETKGLKLNKVEQIKDSEKCLEAIDYDSLSSPSKKIGN 93

Query: 491 LQTIPRE 511
            + I +E
Sbjct: 94  KRKIDQE 100


>SB_2672| Best HMM Match : GSHPx (HMM E-Value=4.1e-14)
          Length = 444

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 18/48 (37%), Positives = 28/48 (58%), Gaps = 4/48 (8%)
 Frame = +3

Query: 312 SAKFPIEREVILSSGEKNSKVPILQEARKRGRL---GAVAPD-TPPKT 443
           S KFPI R+V+L+ GE+ + + + +    +G L   G VA +  PP T
Sbjct: 209 SPKFPILRKVVLNGGERRAYLHVPKGKEVKGGLKMPGKVAANPLPPST 256


>SB_13605| Best HMM Match : MscL (HMM E-Value=6.60012e-43)
          Length = 177

 Score = 28.7 bits (61), Expect = 4.3
 Identities = 12/44 (27%), Positives = 22/44 (50%)
 Frame = -1

Query: 384 EVLALWNSFLLKIISLLVL*EILRSVCAGRQVREALGLQAAPPP 253
           EV  LW  FL  +I  +++  ++  +  G    +   ++AAP P
Sbjct: 116 EVAVLWGEFLTNVIDFVIVAFVMFMIVKGVNAMKKKEVEAAPAP 159


>SB_54212| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 622

 Score = 27.5 bits (58), Expect = 9.9
 Identities = 14/45 (31%), Positives = 20/45 (44%), Gaps = 1/45 (2%)
 Frame = +3

Query: 492 YRQYHENY-NYNMQQNQSGTRNGNYDSPKPPVPAKTYGTKIDATN 623
           Y+    NY N +     +GT    + S K  VP K    ++D TN
Sbjct: 239 YKSQSRNYDNTHSTHTSNGTHQHVHQSAKRKVPVKAPKVRLDGTN 283


>SB_25209| Best HMM Match : Astacin (HMM E-Value=7.69999e-41)
          Length = 533

 Score = 27.5 bits (58), Expect = 9.9
 Identities = 13/22 (59%), Positives = 15/22 (68%)
 Frame = +2

Query: 362 EFQSANTSRGSKTRQTRRRGSR 427
           EF    T+R SK R+ RRRGSR
Sbjct: 56  EFNRLPTNRTSKYRRIRRRGSR 77


>SB_971| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 783

 Score = 27.5 bits (58), Expect = 9.9
 Identities = 11/35 (31%), Positives = 21/35 (60%)
 Frame = +3

Query: 495 RQYHENYNYNMQQNQSGTRNGNYDSPKPPVPAKTY 599
           R+   ++N N + N  G ++GNYD P+   P +++
Sbjct: 455 REGSNSFNRNWR-NDDGYQSGNYDRPENNTPQRSF 488


>SB_55819| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 2408

 Score = 27.5 bits (58), Expect = 9.9
 Identities = 15/48 (31%), Positives = 23/48 (47%)
 Frame = +3

Query: 444  LSAWTHSENQAGTSADYRQYHENYNYNMQQNQSGTRNGNYDSPKPPVP 587
            LSA THS    GT+  YR         +++  +  R  + +SP P +P
Sbjct: 1114 LSAMTHSGGTDGTTCVYRVPRPLDRDTLKKLMTNLRRSDTESPIPTLP 1161


>SB_29493| Best HMM Match : PH (HMM E-Value=0.48)
          Length = 1064

 Score = 27.5 bits (58), Expect = 9.9
 Identities = 28/100 (28%), Positives = 47/100 (47%), Gaps = 6/100 (6%)
 Frame = +2

Query: 293 CRPAHTLRKISYRTRSDIIFRRKEFQSAN-TSRGSKTRQT----RRRGSRHPTKNTLSLD 457
           CR A  L + +Y T +    +R E Q+   +SR +  ++T     +RG + P   + S+ 
Sbjct: 343 CRGADVLLENTYVTPAGPPPQRSESQTEGVSSRPNFFKRTWLRISKRGPKRPRPRSRSVP 402

Query: 458 SLGEPSRHIGRLQT-IPRELQLQHAAESEWY*KWQLRFSQ 574
           SL    RH   +   +P   +L     SE + K + RFS+
Sbjct: 403 SLSRRGRHHEEVTAPVPLTPELPSTTTSEVF-KDETRFSR 441


>SB_23980| Best HMM Match : Mur_ligase_C (HMM E-Value=0.63)
          Length = 227

 Score = 27.5 bits (58), Expect = 9.9
 Identities = 14/34 (41%), Positives = 22/34 (64%)
 Frame = +2

Query: 440 NTLSLDSLGEPSRHIGRLQTIPRELQLQHAAESE 541
           NT+SLDS+GE +       T+ R+ QL+H  E++
Sbjct: 97  NTVSLDSMGENTDLTN--FTVTRDSQLRHCVENQ 128


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,949,260
Number of Sequences: 59808
Number of extensions: 497742
Number of successful extensions: 1704
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1553
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1697
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1645141000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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