Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0872
(723 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g55410.1 68416.m06154 2-oxoglutarate dehydrogenase E1 compone... 90 1e-28
At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 compone... 86 2e-27
At2g31400.1 68415.m03837 pentatricopeptide (PPR) repeat-containi... 35 0.047
At5g20230.1 68418.m02408 plastocyanin-like domain-containing pro... 30 1.4
At2g43410.1 68415.m05395 RNA recognition motif (RRM)-containing ... 30 1.8
At1g64450.1 68414.m07306 proline-rich family protein contains pr... 30 1.8
At3g24550.1 68416.m03083 protein kinase family protein contains ... 29 3.1
At2g15530.2 68415.m01778 zinc finger (C3HC4-type RING finger) fa... 29 4.1
At2g15530.1 68415.m01777 zinc finger (C3HC4-type RING finger) fa... 29 4.1
At1g20110.1 68414.m02516 zinc finger (FYVE type) family protein ... 29 4.1
At1g11820.1 68414.m01358 glycosyl hydrolase family 17 protein si... 28 5.5
At4g08390.2 68417.m01386 L-ascorbate peroxidase, stromal (sAPX) ... 28 7.2
At4g08390.1 68417.m01385 L-ascorbate peroxidase, stromal (sAPX) ... 28 7.2
At5g41680.2 68418.m05065 protein kinase family protein contains ... 27 9.5
At5g41680.1 68418.m05064 protein kinase family protein contains ... 27 9.5
At5g31752.1 68418.m03682 hypothetical protein 27 9.5
At4g03610.1 68417.m00496 phosphonate metabolism protein-related ... 27 9.5
At1g56000.1 68414.m06425 amine oxidase-related contains Pfam pro... 27 9.5
At1g55980.1 68414.m06421 expressed protein 27 9.5
At1g55570.1 68414.m06360 multi-copper oxidase type I family prot... 27 9.5
>At3g55410.1 68416.m06154 2-oxoglutarate dehydrogenase E1 component,
putative / oxoglutarate decarboxylase, putative /
alpha-ketoglutaric dehydrogenase, putative similar to
SP|P20967 2-oxoglutarate dehydrogenase E1 component,
mitochondrial precursor (EC 1.2.4.2)
(Alpha-ketoglutarate dehydrogenase) {Saccharomyces
cerevisiae}; contains Pfam profiles PF02779:
Transketolase, pyridine binding domain, PF00676:
Dehydrogenase E1 component
Length = 1017
Score = 89.8 bits (213), Expect(2) = 1e-28
Identities = 50/129 (38%), Positives = 75/129 (58%), Gaps = 4/129 (3%)
Frame = +2
Query: 323 SAGSP-INEKIIDDHLAVQAIIRSYQARGHLAADVDPLGITTATLPELGMRAPSSELIMR 499
+A SP I+ + I + + + ++R+YQ GH+ A +DPLG+ +PE +L +
Sbjct: 104 AATSPGISGQTIQESMRLLLLVRAYQVNGHMKAKLDPLGLEQREIPE--------DLDLA 155
Query: 500 KYFNFDEADMDRVFKL---PSTTFIGEKEKALPLREILNRLEQAYCNNIGIEFMFINSLE 670
Y F EAD+DR F L + F+ E LR IL RLEQAYC NIG E+M I +
Sbjct: 156 LY-GFTEADLDREFFLGVWQMSGFMSENRPVQTLRSILTRLEQAYCGNIGFEYMHIADRD 214
Query: 671 QCNWIRQRM 697
+CNW+R+++
Sbjct: 215 KCNWLREKI 223
Score = 54.4 bits (125), Expect(2) = 1e-28
Identities = 23/40 (57%), Positives = 28/40 (70%)
Frame = +2
Query: 83 STAAEPFLNGSSSAYVETMYNAWLADPNSVHASWDAFFRN 202
S + FL+G+SS Y+E + AW ADPNSV SWD FFRN
Sbjct: 60 SKLTDSFLDGTSSVYLEELQRAWEADPNSVDESWDNFFRN 99
>At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 component,
putative / oxoglutarate decarboxylase, putative /
alpha-ketoglutaric dehydrogenase, putative similar to
SP|P20967 2-oxoglutarate dehydrogenase E1 component,
mitochondrial precursor (EC 1.2.4.2)
(Alpha-ketoglutarate dehydrogenase) {Saccharomyces
cerevisiae}; contains Pfam profiles PF02779:
Transketolase, pyridine binding domain, PF00676:
Dehydrogenase E1 component
Length = 1025
Score = 85.8 bits (203), Expect(2) = 2e-27
Identities = 50/133 (37%), Positives = 73/133 (54%), Gaps = 3/133 (2%)
Frame = +2
Query: 308 GMPSISAGSPINEKIIDDHLAVQAIIRSYQARGHLAADVDPLGITTATLPELGMRAPSSE 487
G S S G I+ + I + + + ++R+YQ GH+ A +DPLG+ +PE +
Sbjct: 105 GQASTSPG--ISGQTIQESMRLLLLVRAYQVNGHMKAKLDPLGLEKREIPE--------D 154
Query: 488 LIMRKYFNFDEADMDRVFKLP---STTFIGEKEKALPLREILNRLEQAYCNNIGIEFMFI 658
L Y F EAD+DR F L + F+ E LR IL+RLEQAYC IG E+M I
Sbjct: 155 LTPGLY-GFTEADLDREFFLGVWRMSGFLSENRPVQTLRSILSRLEQAYCGTIGYEYMHI 213
Query: 659 NSLEQCNWIRQRM 697
++CNW+R ++
Sbjct: 214 ADRDKCNWLRDKI 226
Score = 54.8 bits (126), Expect(2) = 2e-27
Identities = 28/63 (44%), Positives = 36/63 (57%)
Frame = +2
Query: 14 FASWLLNKPQTAAVSVNANRLKSSTAAEPFLNGSSSAYVETMYNAWLADPNSVHASWDAF 193
F S +L +A V + S + FL+G+SS Y+E + AW ADPNSV SWD F
Sbjct: 41 FHSTILKSKAESAAPV-PRPVPLSKLTDSFLDGTSSVYLEELQRAWEADPNSVDESWDNF 99
Query: 194 FRN 202
FRN
Sbjct: 100 FRN 102
>At2g31400.1 68415.m03837 pentatricopeptide (PPR) repeat-containing
protein low similarity to fertility restorer [Petunia x
hybrida] GI:22128587, post-transcriptional control of
chloroplast gene expression CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 918
Score = 35.1 bits (77), Expect = 0.047
Identities = 24/66 (36%), Positives = 31/66 (46%)
Frame = +3
Query: 36 NHRLPRFQ*MPIG*RAPPRQNPSSMAPVQLTSRPCTMHGSPTQTPYTRLGMHFSAMRQTE 215
NHR P G APP + +++AP QL+ P + SP QTP + L FS R T
Sbjct: 88 NHRPYGASSSPRG-SAPPPSSVATVAPAQLSQPP---NFSPLQTPKSDLSSDFSGRRSTR 143
Query: 216 LSQAPH 233
H
Sbjct: 144 FVSKMH 149
>At5g20230.1 68418.m02408 plastocyanin-like domain-containing
protein
Length = 196
Score = 30.3 bits (65), Expect = 1.4
Identities = 19/46 (41%), Positives = 23/46 (50%), Gaps = 2/46 (4%)
Frame = +2
Query: 203 ATNGAQPGAAYTPPPNLAPYNKNEVPLTSLVPSSGG--MPSISAGS 334
AT GA PGA TP P P P P++GG PS S+G+
Sbjct: 128 ATGGATPGAGATPAPGSTPSTGGTTP-----PTAGGTTTPSGSSGT 168
>At2g43410.1 68415.m05395 RNA recognition motif (RRM)-containing
protein contains InterPro entry IPR000504: RNA-binding
region RNP-1 (RNA recognition motif) (RRM)
Length = 1056
Score = 29.9 bits (64), Expect = 1.8
Identities = 16/53 (30%), Positives = 21/53 (39%)
Frame = +2
Query: 131 ETMYNAWLADPNSVHASWDAFFRNATNGAQPGAAYTPPPNLAPYNKNEVPLTS 289
E AW P +VH + + F+ N P PPP+ P N TS
Sbjct: 841 EAPSQAWKRGPQTVHDASNQSFQQYGNQYTPAGQLPPPPSRYPPASNNPNYTS 893
>At1g64450.1 68414.m07306 proline-rich family protein contains
proline rich extensins, INTERPRO:IPR0002965
Length = 342
Score = 29.9 bits (64), Expect = 1.8
Identities = 31/111 (27%), Positives = 49/111 (44%), Gaps = 2/111 (1%)
Frame = +2
Query: 35 KPQTAAVSVNANRLKSSTAAEPFLNGSSSAYVETMYNAWLADPN-SVHASWDAFFRNATN 211
KP+ +SVNA +L S + N S S YV + +PN +V + +D+ + +
Sbjct: 42 KPKDPKISVNAVQLPSFAVSNNTANFSFSQYVA------VRNPNRAVFSHYDSSIQLLYS 95
Query: 212 GAQPGAAYTPPPNLAPYNKNEVPLTSLVPSSG-GMPSISAGSPINEKIIDD 361
G Q G + P + + T V S PS SA S ++ +I D
Sbjct: 96 GNQVGFMFIPAGKIDSGRIQYMAATFTVHSFPISPPSSSAISTVSAAVIPD 146
>At3g24550.1 68416.m03083 protein kinase family protein contains
Pfam domain PF00069: Protein kinase domain
Length = 652
Score = 29.1 bits (62), Expect = 3.1
Identities = 19/49 (38%), Positives = 23/49 (46%), Gaps = 3/49 (6%)
Frame = +2
Query: 200 NATNGAQPGAAYTPPPNLAPYNKNEVP---LTSLVPSSGGMPSISAGSP 337
N+T P AA +PPP P + P TS PSS PS+ SP
Sbjct: 21 NSTTTTPPPAASSPPPTTTPSSPPPSPSTNSTSPPPSSPLPPSLPPPSP 69
>At2g15530.2 68415.m01778 zinc finger (C3HC4-type RING finger)
family protein similar to Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 704
Score = 28.7 bits (61), Expect = 4.1
Identities = 18/50 (36%), Positives = 25/50 (50%), Gaps = 3/50 (6%)
Frame = +2
Query: 200 NATNGAQPGAAYTPPPNLAPYN---KNEVPLTSLVPSSGGMPSISAGSPI 340
+A + QP + PP N P+N +E+ SL PS PS S G P+
Sbjct: 446 SAIHTPQPNPTWIPPQNAPPHNPSRTSELSPWSLFPSIES-PSASHGGPL 494
>At2g15530.1 68415.m01777 zinc finger (C3HC4-type RING finger)
family protein similar to Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 704
Score = 28.7 bits (61), Expect = 4.1
Identities = 18/50 (36%), Positives = 25/50 (50%), Gaps = 3/50 (6%)
Frame = +2
Query: 200 NATNGAQPGAAYTPPPNLAPYN---KNEVPLTSLVPSSGGMPSISAGSPI 340
+A + QP + PP N P+N +E+ SL PS PS S G P+
Sbjct: 446 SAIHTPQPNPTWIPPQNAPPHNPSRTSELSPWSLFPSIES-PSASHGGPL 494
>At1g20110.1 68414.m02516 zinc finger (FYVE type) family protein
contains Pfam profile: PF01363 FYVE zinc finger
Length = 601
Score = 28.7 bits (61), Expect = 4.1
Identities = 29/102 (28%), Positives = 40/102 (39%), Gaps = 7/102 (6%)
Frame = +2
Query: 38 PQTAAVSVNANRLKSSTAAEP---FLNGSSSAYVETMYNAWLADPNSVHASWDAFFRNAT 208
P A S+N N + P S+Y A P S H + + ++ T
Sbjct: 96 PSPPATSLNPNSYSTFNQPPPPPTIHPQPLSSYGSFDSTAPYQQPTSQHMYYSPYDQHQT 155
Query: 209 NGAQ----PGAAYTPPPNLAPYNKNEVPLTSLVPSSGGMPSI 322
+G P +A P PN APY+ + L S P S G SI
Sbjct: 156 SGYSSAPPPSSAPAPNPNPAPYSSS---LYSAPPYSSGGSSI 194
>At1g11820.1 68414.m01358 glycosyl hydrolase family 17 protein
similar to elicitor inducible chitinase Nt-SubE76
GI:11071974 from [Nicotiana tabacum]
Length = 511
Score = 28.3 bits (60), Expect = 5.5
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +2
Query: 113 SSSAYVETMYNAWLADPNSVHASWDAFFRNAT 208
+SS Y+ ++N L P ASW F+ N+T
Sbjct: 327 TSSVYIYELFNEDLRAPPVSEASWGLFYGNST 358
>At4g08390.2 68417.m01386 L-ascorbate peroxidase, stromal (sAPX)
identical to stromal ascorbate peroxidase [Arabidopsis
thaliana] gi|1419388|emb|CAA67425
Length = 372
Score = 27.9 bits (59), Expect = 7.2
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +2
Query: 26 LLNKPQTAAVSVNANRLKSSTAAEPFLNGSSSAYVETM 139
LL+ P T + ++ L+S+TAA L SSS+ T+
Sbjct: 13 LLSPPPTTTTTTMSSSLRSTTAASLLLRSSSSSSRSTL 50
>At4g08390.1 68417.m01385 L-ascorbate peroxidase, stromal (sAPX)
identical to stromal ascorbate peroxidase [Arabidopsis
thaliana] gi|1419388|emb|CAA67425
Length = 372
Score = 27.9 bits (59), Expect = 7.2
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +2
Query: 26 LLNKPQTAAVSVNANRLKSSTAAEPFLNGSSSAYVETM 139
LL+ P T + ++ L+S+TAA L SSS+ T+
Sbjct: 13 LLSPPPTTTTTTMSSSLRSTTAASLLLRSSSSSSRSTL 50
>At5g41680.2 68418.m05065 protein kinase family protein contains
protein kinase domain, Pfam:PF00069; similar to
receptor-like protein kinase (GI:4008006) [Arabidopsis
thaliana]; similar to receptor-like kinase RHG1
(GI:21239380) (GI:21239382) [Glycine max]
Length = 333
Score = 27.5 bits (58), Expect = 9.5
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = -2
Query: 236 CMRRLAELRLSHCGKMHPKTRVRSLGRRAMHCTWSRRKLNWS 111
C + +L L+H K P+T +RS G A T +R+ +S
Sbjct: 194 CYGCICDLGLTHITKSLPQTTLRSSGYHAPEITDTRKSTQFS 235
>At5g41680.1 68418.m05064 protein kinase family protein contains
protein kinase domain, Pfam:PF00069; similar to
receptor-like protein kinase (GI:4008006) [Arabidopsis
thaliana]; similar to receptor-like kinase RHG1
(GI:21239380) (GI:21239382) [Glycine max]
Length = 359
Score = 27.5 bits (58), Expect = 9.5
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = -2
Query: 236 CMRRLAELRLSHCGKMHPKTRVRSLGRRAMHCTWSRRKLNWS 111
C + +L L+H K P+T +RS G A T +R+ +S
Sbjct: 220 CYGCICDLGLTHITKSLPQTTLRSSGYHAPEITDTRKSTQFS 261
>At5g31752.1 68418.m03682 hypothetical protein
Length = 416
Score = 27.5 bits (58), Expect = 9.5
Identities = 15/48 (31%), Positives = 25/48 (52%)
Frame = +2
Query: 455 PELGMRAPSSELIMRKYFNFDEADMDRVFKLPSTTFIGEKEKALPLRE 598
PE + P+S+ K D+ +++ K T+ E+EKALP+ E
Sbjct: 183 PEAVVALPASDKAADKRVWIDDESSNKMKKKKKKTYGPEEEKALPIFE 230
>At4g03610.1 68417.m00496 phosphonate metabolism protein-related
weak similarity to PhnP protein. (Swiss-Prot:P16692)
[Escherichia coli]
Length = 290
Score = 27.5 bits (58), Expect = 9.5
Identities = 20/60 (33%), Positives = 26/60 (43%), Gaps = 7/60 (11%)
Frame = +2
Query: 320 ISAGSPINEKIIDDHLAVQAI-----IRSYQARGHLAADVDPLGI--TTATLPELGMRAP 478
I G E+II H A+ IRS Q RG D DPL + + T+ + R P
Sbjct: 79 IDVGKSFREQIILTHEHADAVHGLDEIRSLQPRGATIVDTDPLPVFLSQFTMESIATRFP 138
>At1g56000.1 68414.m06425 amine oxidase-related contains Pfam
profile PF01593: amine oxidase, flavin-containing
Length = 384
Score = 27.5 bits (58), Expect = 9.5
Identities = 8/32 (25%), Positives = 18/32 (56%)
Frame = +2
Query: 602 LNRLEQAYCNNIGIEFMFINSLEQCNWIRQRM 697
+N + +A CN G++ MF + + W+ + +
Sbjct: 136 MNSISKALCNESGVKSMFGTGIAKMEWLEEEI 167
>At1g55980.1 68414.m06421 expressed protein
Length = 464
Score = 27.5 bits (58), Expect = 9.5
Identities = 8/32 (25%), Positives = 18/32 (56%)
Frame = +2
Query: 602 LNRLEQAYCNNIGIEFMFINSLEQCNWIRQRM 697
+N + +A CN G++ MF + + W+ + +
Sbjct: 274 MNSISKALCNESGVKSMFGTGIAKMEWLEEEI 305
>At1g55570.1 68414.m06360 multi-copper oxidase type I family protein
nearly identical to pollen-specific BP10 protein
[SP|Q00624][Brassica napus]; contains Multicopper
oxidase domain PF00394
Length = 555
Score = 27.5 bits (58), Expect = 9.5
Identities = 11/25 (44%), Positives = 20/25 (80%)
Frame = -3
Query: 439 DAERVHIGRQVTASLVTPDDSLHGE 365
+AER ++G+Q+ AS+++P+ SL E
Sbjct: 506 NAERRYLGQQLYASVLSPEKSLRDE 530
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,281,981
Number of Sequences: 28952
Number of extensions: 381387
Number of successful extensions: 1213
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1206
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1575119672
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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