Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0854
(647 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g32720.1 68415.m04004 cytochrome b5, putative similar to Cyto... 96 2e-20
At1g26340.1 68414.m03212 cytochrome b5, putative similar to cyto... 92 3e-19
At5g53560.1 68418.m06655 cytochrome b5 isoform 1 identical to SP... 92 3e-19
At2g46650.1 68415.m05820 cytochrome b5, putative similar to cyto... 91 8e-19
At5g48810.1 68418.m06039 cytochrome b5 identical to cytochrome b... 88 4e-18
At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to ... 88 5e-18
At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to ... 86 2e-17
At1g60660.1 68414.m06829 cytochrome b5 domain-containing protein... 63 2e-10
At5g09680.1 68418.m01120 cytochrome b5 domain-containing protein... 61 5e-10
At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putati... 54 6e-08
At3g61580.1 68416.m06897 delta-8 sphingolipid desaturase (SLD1) ... 52 4e-07
At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibit... 33 0.16
At4g09440.1 68417.m01554 hypothetical protein contains Pfam PF04... 28 4.7
At3g48190.1 68416.m05257 ataxia-telangiectasia mutated protein (... 28 4.7
At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical... 27 8.1
At3g44820.1 68416.m04829 phototropic-responsive NPH3 family prot... 27 8.1
At2g31970.1 68415.m03906 DNA repair-recombination protein (RAD50... 27 8.1
>At2g32720.1 68415.m04004 cytochrome b5, putative similar to
Cytochrome B5 SP:P49098 from [Nicotiana tabacum]
Length = 134
Score = 96.3 bits (229), Expect = 2e-20
Identities = 41/78 (52%), Positives = 54/78 (69%)
Frame = +1
Query: 151 SKNLTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGH 330
+K TL EV +H WI+I+ VY+VTKFLE+HPGG D LL GKD T FEDVGH
Sbjct: 5 AKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGH 64
Query: 331 SDDARELLKKYKIGTLPP 384
S+ ARE++++Y +G + P
Sbjct: 65 SESAREMMEQYYVGEIDP 82
>At1g26340.1 68414.m03212 cytochrome b5, putative similar to
cytochrome b5 GB:BAA74839 GI:4240120 from [Arabidopsis
thaliana]
Length = 135
Score = 92.3 bits (219), Expect = 3e-19
Identities = 39/77 (50%), Positives = 53/77 (68%)
Frame = +1
Query: 148 MSKNLTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVG 327
++K +++E H + W++I VYDV+ +++EHPGG D LL VAGKD T FED G
Sbjct: 4 LTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAG 63
Query: 328 HSDDARELLKKYKIGTL 378
HS DAREL++KY IG L
Sbjct: 64 HSKDARELMEKYFIGEL 80
>At5g53560.1 68418.m06655 cytochrome b5 isoform 1 identical to
SP|Q42342 Cytochrome b5 isoform 1 [Arabidopsis thaliana]
Length = 134
Score = 91.9 bits (218), Expect = 3e-19
Identities = 40/75 (53%), Positives = 51/75 (68%)
Frame = +1
Query: 154 KNLTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGHS 333
K L+ +EV KH K W+II VYDVT F+++HPGG + LL GKD T FEDVGHS
Sbjct: 6 KVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVGHS 65
Query: 334 DDARELLKKYKIGTL 378
D AR+++ KY IG +
Sbjct: 66 DTARDMMDKYFIGEI 80
>At2g46650.1 68415.m05820 cytochrome b5, putative similar to
cytochome b5 GI:2695711 from [Olea europaea]
Length = 132
Score = 90.6 bits (215), Expect = 8e-19
Identities = 39/75 (52%), Positives = 53/75 (70%)
Frame = +1
Query: 148 MSKNLTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVG 327
M+ ++ +V KHK + WI+IH VYD++ F++EHPGG + LL V GKD + FEDV
Sbjct: 1 MANLISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVN 60
Query: 328 HSDDARELLKKYKIG 372
HS DA+EL+KKY IG
Sbjct: 61 HSKDAKELMKKYCIG 75
>At5g48810.1 68418.m06039 cytochrome b5 identical to cytochrome b5
[Arabidopsis thaliana] GI:4240122; strong similarity to
Cytochrome B5 SP:P49098 from [Nicotiana tabacum]
Length = 140
Score = 88.2 bits (209), Expect = 4e-18
Identities = 38/75 (50%), Positives = 49/75 (65%)
Frame = +1
Query: 154 KNLTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGHS 333
K TL EV +H K WI+I VYDVTKFL++HPGG + +L GKD T FEDVGHS
Sbjct: 6 KVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHS 65
Query: 334 DDARELLKKYKIGTL 378
A+ +L +Y +G +
Sbjct: 66 STAKAMLDEYYVGDI 80
>At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to
SP|P11832 Nitrate reductase 1 (formerly EC 1.6.6.1)
(NR1){Arabidopsis thaliana}
Length = 917
Score = 87.8 bits (208), Expect = 5e-18
Identities = 39/76 (51%), Positives = 54/76 (71%)
Frame = +1
Query: 151 SKNLTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGH 330
SK ++ EV+KH S WII+H +YD T+FL++HPGG DS+L AG D T+ FE + H
Sbjct: 545 SKMYSISEVRKHNTADSAWIIVHGHIYDCTRFLKDHPGGTDSILINAGTDCTEEFEAI-H 603
Query: 331 SDDARELLKKYKIGTL 378
SD A++LL+ Y+IG L
Sbjct: 604 SDKAKKLLEDYRIGEL 619
>At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to
SP|P11035 Nitrate reductase 2 (formerly EC 1.6.6.1)
(NR2) {Arabidopsis thaliana}
Length = 917
Score = 86.2 bits (204), Expect = 2e-17
Identities = 38/76 (50%), Positives = 54/76 (71%)
Frame = +1
Query: 151 SKNLTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGH 330
+K ++ EVKKH S WII+H +YD T+FL +HPGG+DS+L AG D T+ FE + H
Sbjct: 542 AKMYSMSEVKKHNSADSCWIIVHGHIYDCTRFLMDHPGGSDSILINAGTDCTEEFEAI-H 600
Query: 331 SDDARELLKKYKIGTL 378
SD A+++L+ Y+IG L
Sbjct: 601 SDKAKKMLEDYRIGEL 616
>At1g60660.1 68414.m06829 cytochrome b5 domain-containing protein
contains InterPro accession IPR001199: Cytochrome b5
Length = 121
Score = 62.9 bits (146), Expect = 2e-10
Identities = 29/75 (38%), Positives = 44/75 (58%)
Frame = +1
Query: 154 KNLTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGHS 333
K+ + EV H WIII + VYD+T ++EEHPGG D++L+ AG D T F H+
Sbjct: 47 KSYSKSEVAVHNKRNDCWIIIKDKVYDITSYVEEHPGG-DAILDHAGDDSTDGFFGPQHA 105
Query: 334 DDARELLKKYKIGTL 378
++++ + IG L
Sbjct: 106 TRVFDMIEDFYIGEL 120
>At5g09680.1 68418.m01120 cytochrome b5 domain-containing protein
flavohemoprotein b5+b5R, Homo sapiens, EMBL:AF169803
Length = 211
Score = 61.3 bits (142), Expect = 5e-10
Identities = 26/73 (35%), Positives = 43/73 (58%)
Frame = +1
Query: 160 LTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGHSDD 339
+ +DEVKKH+ S+W ++ VY+++ ++ HPGG D L++ G+DGT F +
Sbjct: 129 IPMDEVKKHRTGDSMWTVLKGRVYNISPYMNFHPGGVDMLMKAVGRDGTLLFNKYHAWVN 188
Query: 340 ARELLKKYKIGTL 378
LL+K +G L
Sbjct: 189 VDILLEKCLVGVL 201
>At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putative
similar to delta-8 sphingolipid desaturase GI:3819708
from [Brassica napus]
Length = 449
Score = 54.4 bits (125), Expect = 6e-08
Identities = 22/52 (42%), Positives = 35/52 (67%)
Frame = +1
Query: 160 LTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAF 315
+T +++KKH +WI I VYDV+ +++ HPGG ++L +AG+D T AF
Sbjct: 10 VTSEDLKKHNKPGDLWISIQGKVYDVSDWVKSHPGGEAAILNLAGQDVTDAF 61
>At3g61580.1 68416.m06897 delta-8 sphingolipid desaturase (SLD1)
identical to delta-8 sphingolipid desaturase GI:3819710
from [Arabidopsis thaliana]; contains Pfam profile
PF00487: Fatty acid desaturase; contains Pfam profile
PF00173: Heme/Steroid binding domain
Length = 449
Score = 51.6 bits (118), Expect = 4e-07
Identities = 21/54 (38%), Positives = 34/54 (62%)
Frame = +1
Query: 154 KNLTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAF 315
K +T +++KKH +WI I VY+V+ +++ HPGG +L + G+D T AF
Sbjct: 8 KYITNEDLKKHNKSGDLWIAIQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAF 61
>At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibitor
family protein low similarity to extensin [Volvox
carteri] GI:21992
Length = 312
Score = 33.1 bits (72), Expect = 0.16
Identities = 23/58 (39%), Positives = 29/58 (50%)
Frame = -1
Query: 422 NFEQSVMILHLSPGGNVPILYFFNNSRASSLCPTSSKAWVPSFPATSNNESAPPGCSS 249
N ILHLS + IL F S +SSL P+SS + P +S+ SAPP S
Sbjct: 2 NILSQTQILHLS----IAILLFITTS-SSSLSPSSSSPSLSPSPPSSSPSSAPPSSLS 54
>At4g09440.1 68417.m01554 hypothetical protein contains Pfam PF04510
: Family of unknown function (DUF577); common family
comprised of At4g09440, At1g66000, At1g66060
Length = 177
Score = 28.3 bits (60), Expect = 4.7
Identities = 13/47 (27%), Positives = 23/47 (48%), Gaps = 2/47 (4%)
Frame = +1
Query: 193 EKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGK--DGTQAFEDVG 327
+ S W++ + T L E P A+S+ E+A K D + ++G
Sbjct: 103 DNSCWVLAFTGAFCATIHLREFPSQAESVKEIANKMIDSVRELVEIG 149
>At3g48190.1 68416.m05257 ataxia-telangiectasia mutated protein (Atm)
identical to ataxia-telangiectasia mutated protein (Atm)
[Arabidopsis thaliana] GI:7529272; contains Pfam profile
PF00855: PWWP domain; contains GA donor splice site at
exon 73
Length = 3255
Score = 28.3 bits (60), Expect = 4.7
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +3
Query: 507 EHILRESDDLKSDYQMLMNTE*Y*NVLKYCYVIFEMFYS 623
EHI +S+ L Q+++NT N L+ CYV+ +S
Sbjct: 2644 EHIFTDSNKLTKSKQVMLNTL---NELRMCYVLERSIFS 2679
>At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical to
Ubiquitin-like protein SMT3 SP:P55852 from[Arabidopsis
thaliana]; identical to cDNA SMT3 protein GI:1707371
Length = 100
Score = 27.5 bits (58), Expect = 8.1
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +1
Query: 262 GGADSLLEVAGKDGTQAFEDVGHSDDARELLKKY 363
GGA L+V G+DG + F + S ++L+ Y
Sbjct: 14 GGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAY 47
>At3g44820.1 68416.m04829 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 661
Score = 27.5 bits (58), Expect = 8.1
Identities = 19/49 (38%), Positives = 24/49 (48%)
Frame = +1
Query: 145 KMSKNLTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVA 291
K K + E K D++S+W T LEE PGGAD+ L VA
Sbjct: 89 KCGKLARMYEDSKSTDKQSLW----------TTVLEEFPGGADNFLIVA 127
>At2g31970.1 68415.m03906 DNA repair-recombination protein (RAD50)
identical to DNA repair-recombination protein GI:7110148
from [Arabidopsis thaliana]
Length = 1316
Score = 27.5 bits (58), Expect = 8.1
Identities = 13/33 (39%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = -3
Query: 468 DPYEQGSSQRHHCPFQFRTI-CDDFASFTWRQR 373
+P+E+ + Q H CP R+ D+ ASF +QR
Sbjct: 683 EPFEKRARQEHSCPCCERSFTADEEASFIKKQR 715
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,732,247
Number of Sequences: 28952
Number of extensions: 255561
Number of successful extensions: 752
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 731
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 750
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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