BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0838 (744 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g17180.1 68417.m02584 glycosyl hydrolase family 17 protein si... 29 4.3 At1g28060.1 68414.m03435 small nuclear ribonucleoprotein family ... 28 7.5 At5g10790.1 68418.m01254 ubiquitin-specific protease 22 (UBP22) ... 27 9.9 At4g19490.2 68417.m02867 expressed protein 27 9.9 At4g19490.1 68417.m02866 expressed protein 27 9.9 At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 27 9.9 At1g49580.1 68414.m05559 calcium-dependent protein kinase, putat... 27 9.9 >At4g17180.1 68417.m02584 glycosyl hydrolase family 17 protein similar to 3-glucanase GI:18483232 from [Sorghum bicolor] Length = 475 Score = 28.7 bits (61), Expect = 4.3 Identities = 17/55 (30%), Positives = 24/55 (43%) Frame = -1 Query: 336 FCVDLKTKRTLNCLSQSDFLETLINIQYE*SDWCVQQEAGDTASR*KHVLASCPE 172 F D + K LN + L+ N+QY S WCV + D H+ +C E Sbjct: 327 FSFDGQAKYRLNLGLGNRGLKNAKNVQYLPSRWCVAHPSRDMTQVGDHLRLACSE 381 >At1g28060.1 68414.m03435 small nuclear ribonucleoprotein family protein / snRNP family protein contains similarity to U4/U6 small nuclear ribonucleoprotein hPrp3 [Homo sapiens] gi|2708307|gb|AAC51926 Length = 786 Score = 27.9 bits (59), Expect = 7.5 Identities = 21/56 (37%), Positives = 31/56 (55%), Gaps = 2/56 (3%) Frame = +2 Query: 347 RARSIAPQ*KAARQLRRRHDALVNA-RRDSTEC-ASRRGDEKDAGE*RSPPQRNRA 508 R R +P R+ RR D V++ RRDS +SRRGD +D + R+ +R R+ Sbjct: 14 RDRDSSPDHSPQREGGRRRDRDVDSKRRDSDHYRSSRRGDREDERD-RTKDRRGRS 68 >At5g10790.1 68418.m01254 ubiquitin-specific protease 22 (UBP22) almost identical to ubiquitin-specific protease 22 GI:11993484 [Arabidopsis thaliana], one amino acid difference Length = 557 Score = 27.5 bits (58), Expect = 9.9 Identities = 9/24 (37%), Positives = 15/24 (62%) Frame = -1 Query: 159 IWFILECDWISDVEQPVSLYCACY 88 +W+ + WI++VE+ V C CY Sbjct: 501 LWYRCDDAWINEVEEEVVRGCECY 524 >At4g19490.2 68417.m02867 expressed protein Length = 1054 Score = 27.5 bits (58), Expect = 9.9 Identities = 15/45 (33%), Positives = 23/45 (51%) Frame = +1 Query: 166 YCFGTRREHVFSP*CSVSSLLLNAPVTLLILNVNQSFQEVALRKT 300 YCF R+HV S S++S L T++IL F +++ T Sbjct: 308 YCFRHLRDHVTSSIDSINSELFRENFTIIIL--TSEFMRISIHDT 350 >At4g19490.1 68417.m02866 expressed protein Length = 1054 Score = 27.5 bits (58), Expect = 9.9 Identities = 15/45 (33%), Positives = 23/45 (51%) Frame = +1 Query: 166 YCFGTRREHVFSP*CSVSSLLLNAPVTLLILNVNQSFQEVALRKT 300 YCF R+HV S S++S L T++IL F +++ T Sbjct: 308 YCFRHLRDHVTSSIDSINSELFRENFTIIIL--TSEFMRISIHDT 350 >At2g29210.1 68415.m03550 splicing factor PWI domain-containing protein contains Pfam profile PF01480: PWI domain Length = 878 Score = 27.5 bits (58), Expect = 9.9 Identities = 15/34 (44%), Positives = 16/34 (47%) Frame = +1 Query: 487 PPAAKPCCPLHRAHRPRSPSTRCRAAAVPLSLYR 588 PPA + P A R RSPS R P LYR Sbjct: 364 PPARRRRSPSPPARRRRSPSPPARRRRSPSPLYR 397 >At1g49580.1 68414.m05559 calcium-dependent protein kinase, putative / CDPK, putative similar to calcium/calmodulin-dependent protein kinase CaMK3 [Nicotiana tabacum] gi|16904226|gb|AAL30820; contains protein kinase domain, Pfam:PF00069; contains serine/threonine protein kinase domain, INTERPRO:IPR002290 Length = 606 Score = 27.5 bits (58), Expect = 9.9 Identities = 16/43 (37%), Positives = 20/43 (46%) Frame = +1 Query: 436 RVRKQTGR*KGCGGIEEPPAAKPCCPLHRAHRPRSPSTRCRAA 564 R R+ R G GG + + P L RA P SP+ RAA Sbjct: 61 RHRRNKSRDVGGGGESKSLTSTPLRQLRRAFHPPSPAKHIRAA 103 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,162,332 Number of Sequences: 28952 Number of extensions: 269446 Number of successful extensions: 614 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 597 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 614 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1643603136 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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