Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0836
(680 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 312 1e-85
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 195 3e-50
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 195 3e-50
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 189 1e-48
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 67 1e-11
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 48 4e-06
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 48 4e-06
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 38 0.005
At5g26210.1 68418.m03119 PHD finger family protein contains Pfam... 30 1.6
At5g64220.1 68418.m08067 calmodulin-binding protein similar to a... 29 2.8
At1g67560.1 68414.m07697 lipoxygenase family protein similar to ... 29 2.8
At1g70160.1 68414.m08073 expressed protein similar to hypothetic... 29 3.8
At5g17300.1 68418.m02026 myb family transcription factor similar... 28 5.0
At5g15580.1 68418.m01824 expressed protein unknown protein F14P3... 28 5.0
At1g51930.1 68414.m05854 zinc finger (C3HC4-type RING finger) fa... 28 5.0
At5g41430.1 68418.m05032 zinc finger (C3HC4-type RING finger) fa... 28 6.6
At3g59570.1 68416.m06647 RabGAP/TBC domain-containing protein si... 28 6.6
At1g70890.1 68414.m08179 major latex protein-related / MLP-relat... 28 6.6
At5g38500.1 68418.m04654 hypothetical protein contains Pfam prof... 27 8.7
At5g07990.1 68418.m00930 flavonoid 3'-monooxygenase / flavonoid ... 27 8.7
At3g42790.1 68416.m04474 PHD finger family protein contains PHD-... 27 8.7
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 312 bits (767), Expect = 1e-85
Identities = 146/216 (67%), Positives = 175/216 (81%), Gaps = 1/216 (0%)
Frame = +2
Query: 2 LKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDH-ACIPIKKSDPVVSYRET 178
LKRLAKSDPMV C EESGEHIVAGAGELHLEICLKDL++D I KSDPVVS+RET
Sbjct: 508 LKRLAKSDPMVVCTMEESGEHIVAGAGELHLEICLKDLQDDFMGGAEIIKSDPVVSFRET 567
Query: 179 VAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYD 358
V + S + +SKSPNKHNRL+M+A+PM +GL E ID+GR+ PRDD K R++ L E++ +D
Sbjct: 568 VCDRSTRTVMSKSPNKHNRLYMEARPMEEGLAEAIDDGRIGPRDDPKIRSKILAEEFGWD 627
Query: 359 VTEARKIWCFGPEGTGPNILVDCSKGVQYLNEIKDSVVAGFQWAAKEGVMAEENLRGVRF 538
A+KIW FGPE TGPN++VD KGVQYLNEIKDSVVAGFQWA+KEG +AEEN+RG+ F
Sbjct: 628 KDLAKKIWAFGPETTGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGPLAEENMRGICF 687
Query: 539 NIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAHP 646
+ DV LH+DAIHRGGGQ+IPT RR +YA +TA P
Sbjct: 688 EVCDVVLHSDAIHRGGGQVIPTARRVIYASQITAKP 723
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 195 bits (475), Expect = 3e-50
Identities = 93/219 (42%), Positives = 139/219 (63%), Gaps = 4/219 (1%)
Frame = +2
Query: 2 LKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETV 181
L++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K +DPVVS+ ETV
Sbjct: 621 LRKISKSYPLAITKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKVADPVVSFCETV 680
Query: 182 AEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDV 361
E S C +++PNK N++ M A+P+ GL EDI+ G V+ + K + KY++D+
Sbjct: 681 VESSSMKCFAETPNKKNKITMIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDL 740
Query: 362 TEARKIWCFGPEGTGPNILVDCSKGVQ----YLNEIKDSVVAGFQWAAKEGVMAEENLRG 529
AR IW FGP+ GPNIL+D + + + +KDS+V GFQW A+EG + +E +R
Sbjct: 741 LAARSIWAFGPDKQGPNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGAREGPLCDEPIRN 800
Query: 530 VRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAHP 646
V+F I D + + +HRG GQ+IPT RR Y+ L A P
Sbjct: 801 VKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATP 839
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 195 bits (475), Expect = 3e-50
Identities = 93/219 (42%), Positives = 139/219 (63%), Gaps = 4/219 (1%)
Frame = +2
Query: 2 LKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETV 181
L++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K +DPVVS+ ETV
Sbjct: 621 LRKISKSYPLAITKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKVADPVVSFCETV 680
Query: 182 AEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDV 361
E S C +++PNK N++ M A+P+ GL EDI+ G V+ + K + KY++D+
Sbjct: 681 VESSSMKCFAETPNKKNKITMIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDL 740
Query: 362 TEARKIWCFGPEGTGPNILVDCSKGVQ----YLNEIKDSVVAGFQWAAKEGVMAEENLRG 529
AR IW FGP+ GPNIL+D + + + +KDS+V GFQW A+EG + +E +R
Sbjct: 741 LAARSIWAFGPDKQGPNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGAREGPLCDEPIRN 800
Query: 530 VRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAHP 646
V+F I D + + +HRG GQ+IPT RR Y+ L A P
Sbjct: 801 VKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATP 839
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 189 bits (461), Expect = 1e-48
Identities = 92/219 (42%), Positives = 137/219 (62%), Gaps = 4/219 (1%)
Frame = +2
Query: 2 LKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETV 181
L++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K +DPVVS+ ETV
Sbjct: 607 LRKISKSYPLAITKVEESGEHTILGTGELYLDSIIKDLRELYSEVQVKVADPVVSFCETV 666
Query: 182 AEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDV 361
E S C +++PNK N+L M A+P+ GL EDI+ G V+ + + KY++D+
Sbjct: 667 VESSSMKCFAETPNKKNKLTMIAEPLDRGLAEDIENGVVSIDWNRVQLGDFFRTKYDWDL 726
Query: 362 TEARKIWCFGPEGTGPNILVDCSKGVQ----YLNEIKDSVVAGFQWAAKEGVMAEENLRG 529
AR IW FGP+ G NIL+D + + + +KDS+V GFQW A+EG + +E +R
Sbjct: 727 LAARSIWAFGPDKQGTNILLDDTLPTEVDRNLMMGVKDSIVQGFQWGAREGPLCDEPIRN 786
Query: 530 VRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAHP 646
V+F I D + + +HRG GQ+IPT RR Y+ L A P
Sbjct: 787 VKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATP 825
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 66.9 bits (156), Expect = 1e-11
Identities = 30/67 (44%), Positives = 45/67 (67%)
Frame = +2
Query: 2 LKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETV 181
L+ L ++DP V+ GEH++A AGE+HLE C+KDL+E A + ++ S P+VSYRET+
Sbjct: 558 LRLLNRADPFVEITVSARGEHVLAAAGEVHLERCVKDLKERFAKVNLEVSPPLVSYRETI 617
Query: 182 AEESDQL 202
+ L
Sbjct: 618 EGDGSNL 624
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 48.4 bits (110), Expect = 4e-06
Identities = 30/100 (30%), Positives = 54/100 (54%), Gaps = 5/100 (5%)
Frame = +2
Query: 2 LKRLAKSDPMVQC-INEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRET 178
L R K DP + ++ ESG+ I++G GELHL+I ++ + ++ + P V++RET
Sbjct: 482 LNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERMRREYK-VDATVGKPRVNFRET 540
Query: 179 VAE--ESDQLCLSKS--PNKHNRLFMKAQPMPDGLPEDID 286
+ + E D L +S ++ R+ +P+P G E +
Sbjct: 541 ITQRAEFDYLHKKQSGGAGQYGRVTGYVEPLPPGSKEKFE 580
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 48.4 bits (110), Expect = 4e-06
Identities = 30/100 (30%), Positives = 54/100 (54%), Gaps = 5/100 (5%)
Frame = +2
Query: 2 LKRLAKSDPMVQC-INEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRET 178
L R K DP + ++ ESG+ I++G GELHL+I ++ + ++ + P V++RET
Sbjct: 482 LNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERMRREYK-VDATVGKPRVNFRET 540
Query: 179 VAE--ESDQLCLSKS--PNKHNRLFMKAQPMPDGLPEDID 286
+ + E D L +S ++ R+ +P+P G E +
Sbjct: 541 ITQRAEFDYLHKKQSGGAGQYGRVTGYVEPLPPGSKEKFE 580
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 38.3 bits (85), Expect = 0.005
Identities = 21/63 (33%), Positives = 36/63 (57%), Gaps = 1/63 (1%)
Frame = +2
Query: 2 LKRLAKSDPMVQCI-NEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRET 178
L +LA+ DP +EE + ++ G GELHLEI + L+ + + P V+YRE+
Sbjct: 514 LIKLAQEDPSFHFSRDEEMNQTVIEGMGELHLEIIVDRLKREFK-VEANVGAPQVNYRES 572
Query: 179 VAE 187
+++
Sbjct: 573 ISK 575
>At5g26210.1 68418.m03119 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 255
Score = 29.9 bits (64), Expect = 1.6
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = +2
Query: 188 ESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDI 283
E + LCL PN+H + + A+ +P LPE +
Sbjct: 44 EKENLCLYGHPNEHWEVNLPAEEVPPELPEPV 75
>At5g64220.1 68418.m08067 calmodulin-binding protein similar to
anther ethylene-upregulated calmodulin-binding protein
ER1 GI:11612392 from[Nicotiana tabacum]
Length = 1050
Score = 29.1 bits (62), Expect = 2.8
Identities = 24/84 (28%), Positives = 38/84 (45%)
Frame = +2
Query: 245 KAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPNILVD 424
K P+P + +D+ E + + R + E++E D I EG GPNIL +
Sbjct: 610 KEPPLPGTIEKDLTE--------LEAKERLIREEFE-DKLYLWLIHKVTEEGKGPNILDE 660
Query: 425 CSKGVQYLNEIKDSVVAGFQWAAK 496
+GV +L + G+ WA K
Sbjct: 661 DGQGVLHL-----AAALGYDWAIK 679
>At1g67560.1 68414.m07697 lipoxygenase family protein similar to
13-lipoxygenase GB:CAA65269 [Solanum tuberosum],
gi:1654140 [Lycopersicon esculentum]
Length = 917
Score = 29.1 bits (62), Expect = 2.8
Identities = 18/56 (32%), Positives = 28/56 (50%), Gaps = 5/56 (8%)
Frame = +2
Query: 209 SKSPNKHNRLFMKAQPM-----PDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDV 361
SK+ N R+ ++QP PDG+ E ++ V+ R D K + Y+YDV
Sbjct: 204 SKNDNPQARIIFRSQPCLPSETPDGIKELREKDLVSVRGDGKGERKPHERIYDYDV 259
>At1g70160.1 68414.m08073 expressed protein similar to hypothetical
protein GI:4455225 from [Arabidopsis thaliana]
Length = 523
Score = 28.7 bits (61), Expect = 3.8
Identities = 21/81 (25%), Positives = 30/81 (37%)
Frame = +2
Query: 44 NEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVAEESDQLCLSKSPN 223
NE+ E IV + E+ LKD + D + T A E + L K
Sbjct: 272 NEKGEEIIVVIPWDEWWELTLKDNSNPQVALLPLHPDIRAKFNNTAAWEYARSMLGKPYG 331
Query: 224 KHNRLFMKAQPMPDGLPEDID 286
HN +F + D P +D
Sbjct: 332 YHNMIFSWIDTLGDNYPPPLD 352
>At5g17300.1 68418.m02026 myb family transcription factor similar to
CCA1 [Arabidopsis thaliana] GI:4090569; contains Pfam
profile PF00249: Myb-like DNA-binding domain
Length = 387
Score = 28.3 bits (60), Expect = 5.0
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = -3
Query: 192 DSSATVSRYDTTGSDFLIGMQAWSSSRSLRQISRWSSPAPAT 67
D+ + S T GS+ L + + S +RSL +S S PA T
Sbjct: 154 DTQSPTSVLSTVGSEALCSLDSSSPNRSLSPVSSASPPAALT 195
>At5g15580.1 68418.m01824 expressed protein unknown protein F14P3.18
- Arabidopsis thaliana, EMBL:AC009755
Length = 927
Score = 28.3 bits (60), Expect = 5.0
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = +1
Query: 295 RESPR*LQDSRSVSYRKVRIRCYRSP*DLV 384
+E+PR DSRS S+R R C P +LV
Sbjct: 223 KETPRLSLDSRSNSFRSARSSCSPEPQELV 252
>At1g51930.1 68414.m05854 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 132
Score = 28.3 bits (60), Expect = 5.0
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = +2
Query: 41 INEESGEHIVAGAGELHLEICLKDLEEDH 127
I EE G G G+ ICL++ E+DH
Sbjct: 63 IKEEEGGREEEGGGKRFCPICLEEYEDDH 91
>At5g41430.1 68418.m05032 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 161
Score = 27.9 bits (59), Expect = 6.6
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +2
Query: 74 GAGELHLEICLKDLEEDHACIPIKK 148
G E+ ICL++LE+ H I IKK
Sbjct: 111 GFDEIGCSICLEELEDGHEIIRIKK 135
>At3g59570.1 68416.m06647 RabGAP/TBC domain-containing protein
similar to GTPase activating protein [Yarrowia
lipolytica] GI:2370595; contains Pfam profile PF00566:
TBC domain
Length = 720
Score = 27.9 bits (59), Expect = 6.6
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = -2
Query: 178 GLTVRHDRVRLLDWNASMVLLKILKTDLKMEFSSTSDNV 62
G TV+ DRV W +++ +++TD +EF N+
Sbjct: 348 GGTVKEDRVSEWLWTLHRIVVDVVRTDSHLEFYEDPGNL 386
>At1g70890.1 68414.m08179 major latex protein-related / MLP-related
low similarity to major latex protein {Papaver
somniferum}[GI:294062] ; contains Pfam profile PF00407:
Pathogenesis-related protein Bet v I family
Length = 158
Score = 27.9 bits (59), Expect = 6.6
Identities = 18/62 (29%), Positives = 33/62 (53%), Gaps = 1/62 (1%)
Frame = -2
Query: 259 HGLSLHE*TVVLVGRL*ETELVRFLSYG-LTVRHDRVRLLDWNASMVLLKILKTDLKMEF 83
HG LHE VG + + +++ G LTV +++ +D +++ K+L+ DL E+
Sbjct: 44 HGCELHEGDWGKVGSI---VIWKYVHDGKLTVGKNKIEAVDPEKNLITFKVLEGDLMNEY 100
Query: 82 SS 77
S
Sbjct: 101 KS 102
>At5g38500.1 68418.m04654 hypothetical protein contains Pfam profile
PF03754: Domain of unknown function (DUF313)
Length = 411
Score = 27.5 bits (58), Expect = 8.7
Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 2/54 (3%)
Frame = +2
Query: 116 EEDHACIPIKKSDPVVSYRETVAEESD--QLCLSKSPNKHNRLFMKAQPMPDGL 271
EE + +K + S ++TV+EE D + LS P K +K Q P+G+
Sbjct: 25 EEQRRRLLAEKREDSKSQKKTVSEEDDSEKRFLSHVPRKKRSSLVKRQQKPNGV 78
>At5g07990.1 68418.m00930 flavonoid 3'-monooxygenase / flavonoid
3'-hydroxylase (F3'H) / cytochrome P450 75B1 (CYP75B1) /
transparent testa 7 protein (TT7) identical to SP|Q9SD85
Flavonoid 3'-monooxygenase (EC 1.14.13.21) (Flavonoid
3'-hydroxylase) (AtF3'H) (Cytochrome P450 75B1)
(TRANSPARENT TESTA 7 protein) {Arabidopsis thaliana};
similar to gi:10334806, gi:10334808
Length = 513
Score = 27.5 bits (58), Expect = 8.7
Identities = 13/42 (30%), Positives = 20/42 (47%)
Frame = +2
Query: 392 PEGTGPNILVDCSKGVQYLNEIKDSVVAGFQWAAKEGVMAEE 517
P G G I S G++ + + ++V GF W GV E+
Sbjct: 437 PFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEK 478
>At3g42790.1 68416.m04474 PHD finger family protein contains
PHD-finger domain, INTERPRO:IPR001965
Length = 250
Score = 27.5 bits (58), Expect = 8.7
Identities = 16/62 (25%), Positives = 27/62 (43%)
Frame = +2
Query: 92 LEICLKDLEEDHACIPIKKSDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGL 271
+E KD + I + V + + E + LCL PN+ + + A+ +P L
Sbjct: 13 VEEVFKDFKGRRTAIVKALTTDVQEFYQQCDPEKENLCLYGLPNEEWEVNLPAEEVPPEL 72
Query: 272 PE 277
PE
Sbjct: 73 PE 74
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,381,874
Number of Sequences: 28952
Number of extensions: 374560
Number of successful extensions: 1161
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 1116
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1156
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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