Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0811
(631 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces po... 202 3e-53
SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosacch... 180 1e-46
SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces... 89 5e-19
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.10
SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizos... 31 0.14
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 0.97
SPAC4D7.11 |||conserved fungal protein|Schizosaccharomyces pombe... 28 1.3
SPBC530.02 |||membrane transporter|Schizosaccharomyces pombe|chr... 27 3.0
SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyce... 26 3.9
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 6.8
SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large... 25 9.0
SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit ... 25 9.0
>SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 161
Score = 202 bits (494), Expect = 3e-53
Identities = 102/133 (76%), Positives = 119/133 (89%)
Frame = +1
Query: 1 AIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIYGLVVAVLIAGALQ 180
AI+F++ GAAYGTAK+G GI+AM V+RP+LI+K+ IPVVMAGIIAIYGLVV+VLI+G L+
Sbjct: 21 AIVFASFGAAYGTAKAGVGISAMGVLRPDLIVKNTIPVVMAGIIAIYGLVVSVLISGNLK 80
Query: 181 EPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAE 360
+ + LY GFI LGAGL+VG +GLAAGFAIGIVGDAGVRGTAQQPRLFV MILILIFAE
Sbjct: 81 QILS--LYSGFIQLGAGLSVGLAGLAAGFAIGIVGDAGVRGTAQQPRLFVAMILILIFAE 138
Query: 361 VLGLYGLIVAIYL 399
VLGLYGLIVA+ L
Sbjct: 139 VLGLYGLIVALLL 151
>SPAC732.01 |vma11||V-type ATPase proteolipid
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 162
Score = 180 bits (439), Expect = 1e-46
Identities = 84/133 (63%), Positives = 108/133 (81%)
Frame = +1
Query: 1 AIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIYGLVVAVLIAGALQ 180
+++FS LGA YGTA +G GIAA+ RPE++MKS+IPVVM+GII +YGLV++VLIAG +
Sbjct: 21 SMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVMSGIIGVYGLVMSVLIAGDMS 80
Query: 181 EPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAE 360
+Y L+ GFIHL AGLAVG +G+AAG+AIG+VGD GV+ +Q R+FV M+LILIFAE
Sbjct: 81 PDNDYSLFSGFIHLSAGLAVGLTGVAAGYAIGVVGDRGVQSFMRQDRIFVSMVLILIFAE 140
Query: 361 VLGLYGLIVAIYL 399
VLGLYGLIV + L
Sbjct: 141 VLGLYGLIVGLIL 153
Score = 35.9 bits (79), Expect = 0.005
Identities = 16/69 (23%), Positives = 37/69 (53%), Gaps = 1/69 (1%)
Frame = +1
Query: 196 PLYKGFIHL-GAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAEVLGL 372
P+Y F G ++ FS L AG+ + G A +P + + ++ ++ + ++G+
Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVMSGIIGV 66
Query: 373 YGLIVAIYL 399
YGL++++ +
Sbjct: 67 YGLVMSVLI 75
>SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 199
Score = 89.0 bits (211), Expect = 5e-19
Identities = 45/138 (32%), Positives = 74/138 (53%), Gaps = 6/138 (4%)
Frame = +1
Query: 4 IIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIYGLVVAVLIAGALQE 183
+ F +GAA+G GT I +V P + K++I ++ ++AIY L++A++ + + +
Sbjct: 54 VAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIYSLIIAIVFSAKIND 113
Query: 184 --PANY----PLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILI 345
PA + Y GF G+ VG L G +GI G + AQ LFV ++++
Sbjct: 114 INPAGFYTKSHYYTGFALFWGGITVGLCNLICGVCVGITGSSAALADAQDASLFVKVLVV 173
Query: 346 LIFAEVLGLYGLIVAIYL 399
IF VLGL+GLIV + +
Sbjct: 174 EIFGSVLGLFGLIVGLLI 191
Score = 40.3 bits (90), Expect = 2e-04
Identities = 20/58 (34%), Positives = 32/58 (55%)
Frame = +1
Query: 220 LGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAEVLGLYGLIVAI 393
LG V F + A + I I G + + G + PR+ ++ +IF EV+ +Y LI+AI
Sbjct: 48 LGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIYSLIIAI 105
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 31.5 bits (68), Expect = 0.10
Identities = 20/57 (35%), Positives = 28/57 (49%)
Frame = -3
Query: 377 P*RPNTSAKIRIRIIPTNNLGC*AVPRTPASPTMPMAKPAARPENPTAKPAPKWMNP 207
P RP ++A ++ PT +VP P++P MP P+A P A AP NP
Sbjct: 1691 PVRPQSAAPPQMSA-PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPAC212.04c |||S. pombe specific DUF999 family protein
1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 288
Score = 31.1 bits (67), Expect = 0.14
Identities = 21/68 (30%), Positives = 30/68 (44%)
Frame = +1
Query: 103 IIPVVMAGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIV 282
II +AG+IA + +++ IAG + G ++ G L LA GF I
Sbjct: 193 IITATIAGVIAAFSVIITATIAGVIAAMV------GILYFGHWLVYKILILAFGFKIVTS 246
Query: 283 GDAGVRGT 306
GD V T
Sbjct: 247 GDVCVSNT 254
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 28.3 bits (60), Expect = 0.97
Identities = 26/65 (40%), Positives = 30/65 (46%), Gaps = 2/65 (3%)
Frame = -3
Query: 308 AVPRTPAS--PTMPMAKPAARPENPTAKPAPKWMNPL*RG*LAGSWRAPAIRTATTRP*M 135
A P P S P++PM PAA P P+A AP PL AG AP + A P
Sbjct: 427 APPSLPPSAPPSLPMGAPAAPPLPPSAPIAP----PL----PAGMPAAPPLPPAAPAPPP 478
Query: 134 AIIPA 120
A PA
Sbjct: 479 APAPA 483
>SPAC4D7.11 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 281
Score = 27.9 bits (59), Expect = 1.3
Identities = 17/39 (43%), Positives = 25/39 (64%), Gaps = 3/39 (7%)
Frame = -2
Query: 405 CVQVDGDD-KSV--KTQYFSENKNKNHSDE*PRLLSSTT 298
C++VD +D K + K+QY +EN N N + P L S+TT
Sbjct: 233 CIEVDSEDWKDLVWKSQYATENANTNSINNSP-LSSNTT 270
>SPBC530.02 |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 541
Score = 26.6 bits (56), Expect = 3.0
Identities = 10/19 (52%), Positives = 12/19 (63%)
Frame = +1
Query: 553 VYYICSLLVFTYLQTGAPC 609
V Y C+LL+FT Q G C
Sbjct: 166 VVYFCTLLMFTLFQIGGGC 184
>SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 760
Score = 26.2 bits (55), Expect = 3.9
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = -2
Query: 216 DEPFVKGVVGWLLEGTSNQDSHDQTVDGNNTRHDDRND 103
DEPF+K +L+ SN++S+ VD N+ D +D
Sbjct: 48 DEPFLKSKYMDILQKISNRESNVINVDLNDLYEFDPSD 85
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 25.4 bits (53), Expect = 6.8
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 4/34 (11%)
Frame = -3
Query: 308 AVPRTPASPTMPMA----KPAARPENPTAKPAPK 219
+VP+ PA+P +P A +P A P P AP+
Sbjct: 513 SVPQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQ 546
>SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large
subunit Rpc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1405
Score = 25.0 bits (52), Expect = 9.0
Identities = 8/19 (42%), Positives = 13/19 (68%)
Frame = -1
Query: 283 PRCLWRNRQPDQRILQPNQ 227
PRCLW +Q +++PN+
Sbjct: 582 PRCLWTGKQVFTVLMKPNR 600
>SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit
Bgs2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1894
Score = 25.0 bits (52), Expect = 9.0
Identities = 10/27 (37%), Positives = 14/27 (51%)
Frame = +2
Query: 362 YWVFTDLSSPSTCTQNKRPEHTPLPSP 442
YW +T +SP+ +N R P P P
Sbjct: 497 YWYYTVFNSPTIIEKNFRQSVGPKPIP 523
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,800,350
Number of Sequences: 5004
Number of extensions: 60978
Number of successful extensions: 199
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 279695522
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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