BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0801
(372 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 29 0.31
SPCC338.16 |pof3||F-box protein Pof3|Schizosaccharomyces pombe|c... 29 0.31
SPAC22F3.13 |tsc1||hamartin|Schizosaccharomyces pombe|chr 1|||Ma... 27 1.2
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 25 3.8
SPBC31F10.13c |hip1|hir1|hira protein Hip1|Schizosaccharomyces p... 25 3.8
SPBC17G9.08c |csx2||Arf GAP protein|Schizosaccharomyces pombe|ch... 25 5.0
SPBC14C8.12 |rpb8||DNA-directed RNA polymerase I, II and III sub... 25 5.0
SPBC11C11.03 |ndc80|ndc10, tid3|spindle pole body protein Ndc80|... 24 6.7
SPBC16A3.12c |||triglyceride lipase-cholesterol esterase |Schizo... 24 6.7
SPAC4G9.04c |||cleavage and polyadenylation specificity factor |... 24 6.7
SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomy... 24 8.8
SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces po... 24 8.8
>SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 670
Score = 28.7 bits (61), Expect = 0.31
Identities = 12/57 (21%), Positives = 28/57 (49%)
Frame = -2
Query: 254 AQLEDLSTTLNLRMAQHEEDLKNAATTDLPHKTIASLSREFAEFKSFVCNAISTLKT 84
+ ++D T +N R+ + E +N +++ +T+ +++ +CN LKT
Sbjct: 519 SSMKDDLTEMNQRLKEQIESYENEVNSEITSRTLKEFETLKTQYEKNLCNLREQLKT 575
>SPCC338.16 |pof3||F-box protein Pof3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 577
Score = 28.7 bits (61), Expect = 0.31
Identities = 22/76 (28%), Positives = 36/76 (47%)
Frame = +1
Query: 76 SI*VFKVLIALHTKDLNSANSRDKLAMVLCGRSVVAAFFKSSSCCAILKFSVVDKSSSCA 255
S+ V+K + LHT+ S DKL + S + F+ C + FS+ K SC
Sbjct: 235 SLKVYKTISPLHTQ------STDKLYTIWTPFSELHYFY----CATPITFSIASKILSCC 284
Query: 256 NSLE*ISMVMIIKFLV 303
L+ + +V +I L+
Sbjct: 285 KKLKQVELVDLIPDLI 300
>SPAC22F3.13 |tsc1||hamartin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 899
Score = 26.6 bits (56), Expect = 1.2
Identities = 25/96 (26%), Positives = 37/96 (38%)
Frame = -2
Query: 320 SSELTQTKNLIIITMDIYSKLFAQLEDLSTTLNLRMAQHEEDLKNAATTDLPHKTIASLS 141
S+ L T + T D +L L L L ++ L+N H I ++
Sbjct: 530 SNNLELTSESLSSTNDTIRRLQRDLLFLQNELRFEKFVRQQHLQNIGKLHREH--ILDMA 587
Query: 140 REFAEFKSFVCNAISTLKTQIELLTLGLDRHEMMSR 33
E K + N K QIELL +D+H S+
Sbjct: 588 VESERQKLLLTN--KRYKAQIELLNSEIDKHRSESQ 621
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 25.0 bits (52), Expect = 3.8
Identities = 14/59 (23%), Positives = 26/59 (44%)
Frame = -2
Query: 341 SHFNNYFSSELTQTKNLIIITMDIYSKLFAQLEDLSTTLNLRMAQHEEDLKNAATTDLP 165
S FN SE I MD++ K +E + NL+ ++ ++ + +T +P
Sbjct: 316 SFFNGQDFSETLSRAKFEEINMDLFKKTLKPVEQVLKDSNLKKSEIDDIVLVGGSTRIP 374
>SPBC31F10.13c |hip1|hir1|hira protein Hip1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 932
Score = 25.0 bits (52), Expect = 3.8
Identities = 9/15 (60%), Positives = 13/15 (86%)
Frame = -2
Query: 179 TTDLPHKTIASLSRE 135
+TD+PH IAS+S+E
Sbjct: 730 STDIPHVVIASISKE 744
>SPBC17G9.08c |csx2||Arf GAP protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 870
Score = 24.6 bits (51), Expect = 5.0
Identities = 10/22 (45%), Positives = 18/22 (81%), Gaps = 1/22 (4%)
Frame = -1
Query: 243 RLVYNTELKNGTTRRR-FEECS 181
R +Y+T++KN +T+++ FEE S
Sbjct: 347 RKIYDTDIKNVSTKKKDFEETS 368
>SPBC14C8.12 |rpb8||DNA-directed RNA polymerase I, II and III
subunit Rpb8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 125
Score = 24.6 bits (51), Expect = 5.0
Identities = 12/40 (30%), Positives = 24/40 (60%)
Frame = -2
Query: 299 KNLIIITMDIYSKLFAQLEDLSTTLNLRMAQHEEDLKNAA 180
+N + +T+DI S+++ +D + +L + + DLK AA
Sbjct: 33 QNDMNLTLDINSQIYPLEKDATFSLQITSNLNSPDLKEAA 72
>SPBC11C11.03 |ndc80|ndc10, tid3|spindle pole body protein
Ndc80|Schizosaccharomyces pombe|chr 2|||Manual
Length = 624
Score = 24.2 bits (50), Expect = 6.7
Identities = 16/57 (28%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
Frame = -2
Query: 284 ITMDIYSKLFAQLEDLSTTLNLRMAQHEEDLKNAATTD-LPHKTIASLSREFAEFKS 117
I++ + K+ ++ E L LN+ ++ E K TD L +I SL ++ +F S
Sbjct: 366 ISISEFEKMVSEREQLDRNLNMIGSKISELRKEVFDTDLLIQASIDSLEKKVQKFNS 422
>SPBC16A3.12c |||triglyceride lipase-cholesterol esterase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 443
Score = 24.2 bits (50), Expect = 6.7
Identities = 7/18 (38%), Positives = 13/18 (72%)
Frame = +3
Query: 222 VQCCRQVFQLCK*FGVNI 275
VQ CR ++++C+ FG +
Sbjct: 69 VQKCRNIYEICEAFGYRV 86
>SPAC4G9.04c |||cleavage and polyadenylation specificity factor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 638
Score = 24.2 bits (50), Expect = 6.7
Identities = 10/30 (33%), Positives = 14/30 (46%)
Frame = +2
Query: 89 SKYLLHYTQRI*IQQTHGINSLWFYVGDLW 178
S + +RI HGIN WF + + W
Sbjct: 444 SDWHFRINKRIRESSLHGINRCWFVMEEEW 473
>SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1201
Score = 23.8 bits (49), Expect = 8.8
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = -2
Query: 209 QHEEDLKNAATTDLPHKTIASLSREFAEFKSF 114
+H+E+ K ++ KT+A + EFAE +F
Sbjct: 1125 EHKEENKKYDNVNI-QKTLAKCAEEFAEHTNF 1155
>SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 949
Score = 23.8 bits (49), Expect = 8.8
Identities = 17/56 (30%), Positives = 25/56 (44%)
Frame = -2
Query: 179 TTDLPHKTIASLSREFAEFKSFVCNAISTLKTQIELLTLGLDRHEMMSRRKVLLLH 12
T D K +A SR EF++ + + TL +++ L LG KVL H
Sbjct: 722 TLDDYGKLLAHFSRLAPEFQTGMLDMFRTLNSRVYQLILGAGTVRSSGLSKVLGKH 777
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,510,494
Number of Sequences: 5004
Number of extensions: 28376
Number of successful extensions: 83
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 81
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 83
length of database: 2,362,478
effective HSP length: 65
effective length of database: 2,037,218
effective search space used: 118158644
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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