Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0723
(372 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to... 52 1e-07
At5g37600.1 68418.m04529 glutamine synthetase, putative similar ... 48 3e-06
At5g16570.1 68418.m01939 glutamine synthetase, putative similar ... 48 3e-06
At1g66200.1 68414.m07514 glutamine synthetase, putative similar ... 46 6e-06
At3g17820.1 68416.m02272 glutamine synthetase (GS1) identical to... 45 1e-05
At1g48470.1 68414.m05418 glutamine synthetase, putative similar ... 45 1e-05
At2g41590.1 68415.m05139 expressed protein similar to zinc finge... 29 1.3
At4g32880.1 68417.m04679 homeobox-leucine zipper transcription f... 28 2.3
At5g19280.1 68418.m02298 kinase associated protein phosphatase (... 27 3.0
At1g53180.1 68414.m06027 expressed protein 27 3.0
At1g32880.1 68414.m04051 importin alpha-1 subunit, putative simi... 27 3.0
At5g37030.1 68418.m04441 tRNA-splicing endonuclease positive eff... 27 4.0
At1g18550.1 68414.m02314 kinesin motor protein-related contains ... 27 4.0
At3g49170.1 68416.m05374 pentatricopeptide (PPR) repeat-containi... 27 5.3
At5g47430.1 68418.m05844 expressed protein 26 7.0
At5g37060.1 68418.m04448 cation/hydrogen exchanger, putative (CH... 26 7.0
At4g11540.1 68417.m01851 DC1 domain-containing protein contains ... 26 7.0
At3g28345.1 68416.m03541 ABC transporter family protein similar ... 26 7.0
At1g61600.1 68414.m06941 expressed protein 26 7.0
At1g61550.1 68414.m06934 S-locus protein kinase, putative simila... 26 7.0
At5g03140.1 68418.m00262 lectin protein kinase family protein co... 26 9.2
At4g28630.1 68417.m04093 ABC transporter family protein identica... 26 9.2
At4g17410.1 68417.m02607 expressed protein 26 9.2
At2g33060.1 68415.m04054 leucine-rich repeat family protein cont... 26 9.2
At1g66170.1 68414.m07511 PHD finger family protein (MMD1) contai... 26 9.2
At1g10910.1 68414.m01253 pentatricopeptide (PPR) repeat-containi... 26 9.2
>At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to
glutamine synthetase, chloroplast precursor (glutamate--
ammonia ligase, GS2) [Arabidopsis thaliana]
SWISS-PROT:Q43127
Length = 430
Score = 52.0 bits (119), Expect = 1e-07
Identities = 29/78 (37%), Positives = 42/78 (53%), Gaps = 1/78 (1%)
Frame = +2
Query: 140 KIEDNPKILSGPVLT-NSPNAVLSKTLLSRYNDLPLPADKILATYIWIDGSGEHLRCKDR 316
K + N K+ VL S N+ +++ D +D+I+A YIWI GSG LR K R
Sbjct: 37 KKQSNNKVRGFRVLALQSDNSTVNRVETLLNLDTKPYSDRIIAEYIWIGGSGIDLRSKSR 96
Query: 317 TLNFIPKAPKDLPIWNFD 370
T+ + P +LP WN+D
Sbjct: 97 TIEKPVEDPSELPKWNYD 114
>At5g37600.1 68418.m04529 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (Glutamate--
ammonia ligase, GS1) [Lotus japonicus] SWISS-PROT:Q42899
Length = 356
Score = 47.6 bits (108), Expect = 3e-06
Identities = 21/40 (52%), Positives = 25/40 (62%)
Frame = +2
Query: 251 DKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFD 370
DKI+A YIW+ GSG +R K RTL P LP WN+D
Sbjct: 17 DKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSQLPKWNYD 56
>At5g16570.1 68418.m01939 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase) [Alfalfa] SWISS-PROT:P04078
Length = 356
Score = 47.6 bits (108), Expect = 3e-06
Identities = 23/46 (50%), Positives = 27/46 (58%)
Frame = +2
Query: 233 DLPLPADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFD 370
DL D+I+A YIWI GSG +R K RTL P LP WN+D
Sbjct: 11 DLSDSTDQIIAEYIWIGGSGLDMRSKARTLPGPVTDPSQLPKWNYD 56
>At1g66200.1 68414.m07514 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (Glutamate--
ammonia ligase, GS1) [Lotus japonicus] SWISS-PROT:Q42899
Length = 356
Score = 46.4 bits (105), Expect = 6e-06
Identities = 21/46 (45%), Positives = 28/46 (60%)
Frame = +2
Query: 233 DLPLPADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFD 370
D+ ++KI+A YIW+ GSG +R K RTL P LP WN+D
Sbjct: 11 DISDNSEKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSKLPKWNYD 56
>At3g17820.1 68416.m02272 glutamine synthetase (GS1) identical to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase, GS1) [Arabidopsis thaliana]
SWISS-PROT:Q9LVI8
Length = 354
Score = 45.2 bits (102), Expect = 1e-05
Identities = 21/39 (53%), Positives = 24/39 (61%)
Frame = +2
Query: 254 KILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFD 370
KI+A YIWI GSG +R K RTL P LP WN+D
Sbjct: 18 KIIAEYIWIGGSGMDIRSKARTLPGPVTDPSKLPKWNYD 56
>At1g48470.1 68414.m05418 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase, GS1) [Arabidopsis thaliana]
SWISS-PROT:Q9LVI8
Length = 353
Score = 45.2 bits (102), Expect = 1e-05
Identities = 21/39 (53%), Positives = 24/39 (61%)
Frame = +2
Query: 254 KILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFD 370
KI+A YIWI GSG +R K RTL P LP WN+D
Sbjct: 18 KIIAEYIWIGGSGMDIRSKARTLPGPVSNPTKLPKWNYD 56
>At2g41590.1 68415.m05139 expressed protein similar to zinc finger
protein [Arabidopsis thaliana] GI:976277
Length = 367
Score = 28.7 bits (61), Expect = 1.3
Identities = 13/36 (36%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
Frame = +2
Query: 224 RYNDLPLPADKILATYIWID-GSGEHLRCKDRTLNF 328
R + PL D+ +A Y +D G +H+RC+ + NF
Sbjct: 263 RISHPPLNHDEFVAAYPHLDSGRNDHIRCEGESSNF 298
>At4g32880.1 68417.m04679 homeobox-leucine zipper transcription
factor (HB-8) identical to HD-zip transcription factor
(athb-8) (GI:7270235) [Arabidopsis thaliana]
Length = 833
Score = 27.9 bits (59), Expect = 2.3
Identities = 14/34 (41%), Positives = 17/34 (50%)
Frame = +1
Query: 271 HLDRRLWRTPEVQRSHLEFHT*SSQRSAYMELRW 372
H D W PEV RS E T +QR+ LR+
Sbjct: 332 HFDLEPWSVPEVLRSLYESSTLLAQRTTMAALRY 365
>At5g19280.1 68418.m02298 kinase associated protein phosphatase
(KAPP) identical to Kinase associated protein
phosphatase (SP:P46014) [Arabidopsis thaliana]; contains
Pfam PF00481: Protein phosphatase 2C domain; contains
Pfam PF00498: FHA domain
Length = 581
Score = 27.5 bits (58), Expect = 3.0
Identities = 18/51 (35%), Positives = 26/51 (50%), Gaps = 7/51 (13%)
Frame = +2
Query: 236 LPLPADK----ILATYIWIDGSGEHLRC---KDRTLNFIPKAPKDLPIWNF 367
L LPA+K + TY W + E+L+ KDR +N P+ +D W F
Sbjct: 133 LKLPAEKGSLAEVQTYDWQNNRNENLQYNLEKDRLINLSPRLVEDQRSWLF 183
>At1g53180.1 68414.m06027 expressed protein
Length = 358
Score = 27.5 bits (58), Expect = 3.0
Identities = 16/39 (41%), Positives = 21/39 (53%)
Frame = -1
Query: 294 SPEPSIQM*VARILSAGSGRSLYRLSSVLDSTAFGELVK 178
SPE + ++L A +GRSL R SS+ DS G K
Sbjct: 49 SPEKMGDSDIGKVLEASTGRSLIRASSI-DSREGGSQTK 86
>At1g32880.1 68414.m04051 importin alpha-1 subunit, putative similar
to importin alpha-1 subunit (Karyopherin alpha-1
subunit, KAP alpha) [Arabidopsis thaliana]
SWISS-PROT:Q96321
Length = 183
Score = 27.5 bits (58), Expect = 3.0
Identities = 17/59 (28%), Positives = 26/59 (44%)
Frame = -1
Query: 267 VARILSAGSGRSLYRLSSVLDSTAFGELVKTGPDRIFGLSSILVCAVSAILKQVRHDYV 91
V R++ S +L SV+D+ LVK + F + VCA+S HD +
Sbjct: 26 VHRLIQFLLDESFPKLQSVIDANLIPTLVKLTQNAEFDMKKESVCAISNATLLGSHDQI 84
>At5g37030.1 68418.m04441 tRNA-splicing endonuclease positive
effector-related contains similarity to SEN1, a positive
effector of tRNA-splicing endonuclease [Saccharomyces
cerevisiae] gi|172574|gb|AAB63976
Length = 638
Score = 27.1 bits (57), Expect = 4.0
Identities = 10/42 (23%), Positives = 23/42 (54%)
Frame = -1
Query: 228 YRLSSVLDSTAFGELVKTGPDRIFGLSSILVCAVSAILKQVR 103
+ + +L+ + + +KT PD+ ++ L C V +L++ R
Sbjct: 16 WSMEDILNKDLYKQKIKTIPDKFSSVNEYLKCFVPHLLEETR 57
>At1g18550.1 68414.m02314 kinesin motor protein-related contains
similarity to kinesin-related protein GI:4493964 from
[Plasmodium falciparum]
Length = 799
Score = 27.1 bits (57), Expect = 4.0
Identities = 26/76 (34%), Positives = 36/76 (47%), Gaps = 5/76 (6%)
Frame = -1
Query: 324 FKVRSLHLRCSPEPSIQM*VARIL-SAGSGRSLYRLSSVLDSTAFGELVKTG----PDRI 160
F +R+ + SP+P Q ++L S+ G +RL DST E K DRI
Sbjct: 52 FSLRNPNHNQSPKPEDQRFKTQLLDSSKKGDRFHRLDGK-DSTFVEEDTKENNVFEADRI 110
Query: 159 FGLSSILVCAVSAILK 112
FG+SS+ V I K
Sbjct: 111 FGVSSVPVKPSGVIRK 126
>At3g49170.1 68416.m05374 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 850
Score = 26.6 bits (56), Expect = 5.3
Identities = 27/86 (31%), Positives = 38/86 (44%), Gaps = 1/86 (1%)
Frame = -1
Query: 291 PEPSIQM*VARILSAGSGRSLYRLSSVLDSTAFGELVKTGPD-RIFGLSSILVCAVSAIL 115
P +I+ + +LS G + LSSV + A E + G + + S LV V L
Sbjct: 250 PREAIRFFLDMVLS-GFESDKFTLSSVFSACAELENLSLGKQLHSWAIRSGLVDDVECSL 308
Query: 114 KQVRHDYVLKCSLDASVEYCAKTYLR 37
D KCS D SV+ C K + R
Sbjct: 309 V----DMYAKCSADGSVDDCRKVFDR 330
>At5g47430.1 68418.m05844 expressed protein
Length = 879
Score = 26.2 bits (55), Expect = 7.0
Identities = 10/33 (30%), Positives = 17/33 (51%), Gaps = 1/33 (3%)
Frame = -2
Query: 320 RCDLCTSGVRQSR-RSKCRWRESCQPAVEDHCI 225
+C LC ++ + SKC ++ C + DH I
Sbjct: 294 KCPLCKEVMKDAALTSKCCYKSFCDKCIRDHII 326
>At5g37060.1 68418.m04448 cation/hydrogen exchanger, putative
(CHX24) similar to Na+/H+-exchanging protein NapA -
Enterococcus hirae, PIR:A42111; monovalent cation:proton
antiporter family 2 (CPA2) member, PMID:11500563
Length = 859
Score = 26.2 bits (55), Expect = 7.0
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = +3
Query: 6 IETFYAPTKPFVNTFSRNIQHS 71
I FY PT+P+ ++ R IQH+
Sbjct: 462 INFFYDPTRPYRSSKHRTIQHT 483
>At4g11540.1 68417.m01851 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 525
Score = 26.2 bits (55), Expect = 7.0
Identities = 15/43 (34%), Positives = 18/43 (41%)
Frame = -2
Query: 347 LWEL*V*NSRCDLCTSGVRQSRRSKCRWRESCQPAVEDHCIDS 219
LWE N RC CT VR C+ C + HC +S
Sbjct: 303 LWEE---NKRCSACTHPVRLQSFYACK---DCDFILHQHCAES 339
>At3g28345.1 68416.m03541 ABC transporter family protein similar to
P-glycoprotein [Arabidopsis thaliana] GI:3849833;
contains Pfam profiles PF00005: ABC transporter, PF00664:
ABC transporter transmembrane region
Length = 1240
Score = 26.2 bits (55), Expect = 7.0
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = +3
Query: 6 IETFYAPTKPFVNTFSRNIQHSHRGNISRH 95
IE FY P K V R+I+ H ++ RH
Sbjct: 1043 IERFYDPLKGIVKIDGRDIRSYHLRSLRRH 1072
>At1g61600.1 68414.m06941 expressed protein
Length = 421
Score = 26.2 bits (55), Expect = 7.0
Identities = 16/44 (36%), Positives = 23/44 (52%)
Frame = +2
Query: 221 SRYNDLPLPADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDL 352
++ LPLP DKIL+ + +D SG + L FIP + L
Sbjct: 51 TKIEKLPLPQDKILSV-VHLDSSGNRETSVKKVL-FIPALDQPL 92
>At1g61550.1 68414.m06934 S-locus protein kinase, putative similar
to receptor protein kinase [Ipomoea trifida]
gi|836954|gb|AAC23542; contains S-locus glycoprotein
family domain, Pfam:PF00954
Length = 802
Score = 26.2 bits (55), Expect = 7.0
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = +2
Query: 191 PNAVLSKTLLSRYNDLPLPADKILATYIWIDGS 289
PN + ++L+ +DLPLP + A + DGS
Sbjct: 751 PNTLELLSMLTTTSDLPLPKEPTFAVHTSDDGS 783
>At5g03140.1 68418.m00262 lectin protein kinase family protein
contains Pfam domains, PF00138: Legume lectins alpha
domain, PF00139: Legume lectins beta domain and PF00069:
Protein kinase domain
Length = 711
Score = 25.8 bits (54), Expect = 9.2
Identities = 17/51 (33%), Positives = 25/51 (49%), Gaps = 1/51 (1%)
Frame = +2
Query: 194 NAVLSKTLLSRYN-DLPLPADKILATYIWIDGSGEHLRCKDRTLNFIPKAP 343
NAV+S + N D+ L + + ++I DGSG L N PK+P
Sbjct: 171 NAVVSAAVADLGNVDIDLKSGNAVNSWITYDGSGRVLTVYVSYSNLKPKSP 221
>At4g28630.1 68417.m04093 ABC transporter family protein identical
to half-molecule ABC transporter ATM1 GI:9964117 from
[Arabidopsis thaliana]
Length = 678
Score = 25.8 bits (54), Expect = 9.2
Identities = 12/46 (26%), Positives = 22/46 (47%)
Frame = -1
Query: 183 VKTGPDRIFGLSSILVCAVSAILKQVRHDYVLKCSLDASVEYCAKT 46
+K P+ F + + L C + A V+ ++ K S+D Y + T
Sbjct: 93 MKDNPELRFRVIAALACLIGAKFLNVQVPFLFKLSIDLLSSYSSST 138
>At4g17410.1 68417.m02607 expressed protein
Length = 744
Score = 25.8 bits (54), Expect = 9.2
Identities = 11/33 (33%), Positives = 16/33 (48%), Gaps = 1/33 (3%)
Frame = -2
Query: 320 RCDLCTSGVRQSR-RSKCRWRESCQPAVEDHCI 225
+C LC +R + SKC + C + DH I
Sbjct: 205 KCPLCKEVMRDAALASKCCLKSYCDKCIRDHII 237
>At2g33060.1 68415.m04054 leucine-rich repeat family protein
contains leucine rich-repeat domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Hcr2-0B
[Lycopersicon esculentum] gi|3894387|gb|AAC78593
Length = 808
Score = 25.8 bits (54), Expect = 9.2
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = +2
Query: 185 NSPNAVLSKTLLSRYNDLPLPADKILATYIWIDGSGEHL 301
N+P + T+ +Y L + K+L +Y ID SG L
Sbjct: 576 NNPYYIYEDTVDLQYKGLFMEQGKVLTSYATIDFSGNKL 614
>At1g66170.1 68414.m07511 PHD finger family protein (MMD1) contains
Pfam profile: PF00628: PHD-finger
Length = 704
Score = 25.8 bits (54), Expect = 9.2
Identities = 22/74 (29%), Positives = 35/74 (47%)
Frame = +2
Query: 17 LRSDKTFRKYVFAQYSTLASREHFKT*SCLTCFKMADTAHTKIEDNPKILSGPVLTNSPN 196
LR K + VF Y + S H KT L F + +H + + + P+LT+SP+
Sbjct: 249 LRQSKEINQ-VFRYYREM-SEGHLKTFRDLLRFMLIIKSHASPQKLLPV-TPPLLTDSPH 305
Query: 197 AVLSKTLLSRYNDL 238
S LL + +D+
Sbjct: 306 QKRSSRLLLKKSDV 319
>At1g10910.1 68414.m01253 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 652
Score = 25.8 bits (54), Expect = 9.2
Identities = 18/72 (25%), Positives = 31/72 (43%), Gaps = 2/72 (2%)
Frame = +2
Query: 29 KTFRKYVFAQYS--TLASREHFKT*SCLTCFKMADTAHTKIEDNPKILSGPVLTNSPNAV 202
+ F Y +YS T+ H K L + A+ ++DN K++S P L A
Sbjct: 488 EAFSVYNMLRYSKRTICKELHEKILHILIQGNLLKDAYIVVKDNAKMISQPTLKKFGRAF 547
Query: 203 LSKTLLSRYNDL 238
+ ++ ND+
Sbjct: 548 MISGNINLVNDV 559
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,715,239
Number of Sequences: 28952
Number of extensions: 177029
Number of successful extensions: 478
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 474
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 478
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 497853200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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