BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0721 (706 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_8083| Best HMM Match : Lectin_C (HMM E-Value=3.8e-22) 31 1.2 SB_41144| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.6 SB_49137| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.4 SB_53775| Best HMM Match : Involucrin2 (HMM E-Value=7.1e-12) 28 8.5 >SB_8083| Best HMM Match : Lectin_C (HMM E-Value=3.8e-22) Length = 3445 Score = 30.7 bits (66), Expect = 1.2 Identities = 20/68 (29%), Positives = 35/68 (51%), Gaps = 5/68 (7%) Frame = +3 Query: 240 KPSVTSIETSTTAKQESTTVVEQLKESPMLNLRSAIPTEILNAPSLV-----PADTGEPL 404 KP+VT STT ++ T + + + P+ L++ PT IL ++V P T P+ Sbjct: 1135 KPTVTP---STTISPKTETSITEATKPPVDQLKTKQPTTILGETTIVPETTIPESTAAPV 1191 Query: 405 DDGITTDV 428 + IT ++ Sbjct: 1192 ETTITPEI 1199 Score = 28.7 bits (61), Expect = 4.9 Identities = 17/60 (28%), Positives = 26/60 (43%) Frame = +3 Query: 252 TSIETSTTAKQESTTVVEQLKESPMLNLRSAIPTEILNAPSLVPADTGEPLDDGITTDVP 431 T++ TT + E++T ++ + A P E PS+ P T P D TT P Sbjct: 252 TTVVPDTTVEPETSTTLKPTDVTEKPEASPATPAEYSTTPSVQPELTEAPQTDYPTTASP 311 >SB_41144| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 159 Score = 30.3 bits (65), Expect = 1.6 Identities = 23/71 (32%), Positives = 34/71 (47%) Frame = +3 Query: 219 DIILTTEKPSVTSIETSTTAKQESTTVVEQLKESPMLNLRSAIPTEILNAPSLVPADTGE 398 D TT + S +T T K TT Q+ ++P+ +P +I +A LVP DT Sbjct: 25 DFCFTTRTTGILSQDTQTPVKT-FTTPDTQILKTPL------VPLDI-SASRLVPLDTQT 76 Query: 399 PLDDGITTDVP 431 PL++ T P Sbjct: 77 PLNNFYNTGYP 87 >SB_49137| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 323 Score = 28.3 bits (60), Expect = 6.4 Identities = 17/51 (33%), Positives = 28/51 (54%), Gaps = 3/51 (5%) Frame = +3 Query: 285 ESTTVVEQLKESPMLNL---RSAIPTEILNAPSLVPADTGEPLDDGITTDV 428 ++T V + E+ L++ RSA+ ++LNA L P D PLD + T + Sbjct: 183 KATIVQPMITEASTLSMCFCRSAMVVKLLNAQELPPKDDTGPLDVFVKTQL 233 >SB_53775| Best HMM Match : Involucrin2 (HMM E-Value=7.1e-12) Length = 1879 Score = 27.9 bits (59), Expect = 8.5 Identities = 13/31 (41%), Positives = 20/31 (64%) Frame = +3 Query: 228 LTTEKPSVTSIETSTTAKQESTTVVEQLKES 320 LT+++ S S+E T+K+ES VEQ + S Sbjct: 1794 LTSKEESAPSVEQQQTSKEESDLCVEQQQTS 1824 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 18,770,986 Number of Sequences: 59808 Number of extensions: 339496 Number of successful extensions: 792 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 720 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 790 length of database: 16,821,457 effective HSP length: 80 effective length of database: 12,036,817 effective search space used: 1853669818 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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