Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0709
(346 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g42190.1 68418.m05135 E3 ubiquitin ligase SCF complex subunit... 70 4e-13
At1g75950.1 68414.m08821 E3 ubiquitin ligase SCF complex subunit... 68 2e-12
At4g34470.1 68417.m04901 E3 ubiquitin ligase SCF complex subunit... 66 4e-12
At4g34210.1 68417.m04856 E3 ubiquitin ligase SCF complex subunit... 66 4e-12
At1g10230.1 68414.m01153 E3 ubiquitin ligase SCF complex subunit... 58 2e-09
At2g03170.1 68415.m00270 E3 ubiquitin ligase SCF complex subunit... 56 5e-09
At1g20140.1 68414.m02519 E3 ubiquitin ligase SCF complex subunit... 54 3e-08
At3g60010.1 68416.m06700 E3 ubiquitin ligase SCF complex subunit... 54 3e-08
At3g25650.1 68416.m03192 Skp1 family protein similar toSkp1 [Med... 54 3e-08
At3g21850.1 68416.m02754 E3 ubiquitin ligase SCF complex subunit... 53 6e-08
At2g03160.1 68415.m00269 E3 ubiquitin ligase SCF complex subunit... 53 6e-08
At3g21830.1 68416.m02752 E3 ubiquitin ligase SCF complex subunit... 52 1e-07
At2g25700.1 68415.m03080 E3 ubiquitin ligase SCF complex subunit... 52 1e-07
At2g03190.1 68415.m00272 E3 ubiquitin ligase SCF complex subunit... 52 1e-07
At3g60020.1 68416.m06702 E3 ubiquitin ligase SCF complex subunit... 51 2e-07
At3g21860.1 68416.m02755 E3 ubiquitin ligase SCF complex subunit... 50 5e-07
At2g20160.1 68415.m02357 E3 ubiquitin ligase SCF complex subunit... 47 4e-06
At3g61415.1 68416.m06878 SKP1 family protein low similarity to S... 35 0.017
At2g45950.1 68415.m05713 SKP1 family protein similar to glycopro... 33 0.067
At1g52970.1 68414.m05992 hypothetical protein 27 2.5
At5g02820.1 68418.m00224 DNA topoisomerase VIA (SPO11) identical... 26 7.7
At4g27970.1 68417.m04013 C4-dicarboxylate transporter/malic acid... 26 7.7
At2g26260.1 68415.m03152 3-beta hydroxysteroid dehydrogenase/iso... 26 7.7
>At5g42190.1 68418.m05135 E3 ubiquitin ligase SCF complex subunit
SKP1/ASK1 (At2) / UFO-binding protein (UIP2) E3
ubiquitin ligase; skp1b; identical to UIP2 GI:3719211
from [Arabidopsis thaliana]; contains Pfam profiles
PF01466: Skp1 family, dimerisation domain and
PF03931:Skp1 family, tetramerisation domain; identical
to cDNA UFO binding protein UIP2 mRNA, partial cds
GI:3719210
Length = 171
Score = 70.1 bits (164), Expect = 4e-13
Identities = 30/39 (76%), Positives = 35/39 (89%)
Frame = +2
Query: 11 ANMIKGKTPEEIRKTFNIKNDFTAAEEDQVRKENEWCEE 127
A+MIKGKTPEEIRKTFNIKNDFT EE++VR+EN+W E
Sbjct: 133 ADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 171
>At1g75950.1 68414.m08821 E3 ubiquitin ligase SCF complex subunit
SKP1/ASK1 (At1) E3 ubiquitin ligase; skp1a; identical to
Skp1a GI:3068807, Skp1p GI:1432083 and UIP1 GI:3719209
from [Arabidopsis thaliana]; contains Pfam profiles
PF01466: Skp1 family, dimerisation domain and PF03931:
Skp1 family, tetramerisation domain;
Length = 160
Score = 67.7 bits (158), Expect = 2e-12
Identities = 29/39 (74%), Positives = 34/39 (87%)
Frame = +2
Query: 11 ANMIKGKTPEEIRKTFNIKNDFTAAEEDQVRKENEWCEE 127
A+MIKGKTPEEIR TFNIKNDFT EE++VR+EN+W E
Sbjct: 122 ADMIKGKTPEEIRTTFNIKNDFTPEEEEEVRRENQWAFE 160
>At4g34470.1 68417.m04901 E3 ubiquitin ligase SCF complex subunit
SKP1/ASK1 (At12), putative E3 ubiquitin ligase; similar
to Skp1 homolog Skp1a GI:3068807 from [Arabidopsis
thaliana]
Length = 152
Score = 66.5 bits (155), Expect = 4e-12
Identities = 29/39 (74%), Positives = 33/39 (84%)
Frame = +2
Query: 11 ANMIKGKTPEEIRKTFNIKNDFTAAEEDQVRKENEWCEE 127
A+MIKGKTPEEIR TFNI+NDFT EE+ VRKEN+W E
Sbjct: 114 ADMIKGKTPEEIRSTFNIENDFTPEEEEAVRKENQWAFE 152
>At4g34210.1 68417.m04856 E3 ubiquitin ligase SCF complex subunit
SKP1/ASK1 (At11), putative E3 ubiquitin ligase; similar
to Skp1 homolog Skp1a GI:3068807 from [Arabidopsis
thaliana]
Length = 152
Score = 66.5 bits (155), Expect = 4e-12
Identities = 29/39 (74%), Positives = 33/39 (84%)
Frame = +2
Query: 11 ANMIKGKTPEEIRKTFNIKNDFTAAEEDQVRKENEWCEE 127
A+MIKGKTPEEIR TFNI+NDFT EE+ VRKEN+W E
Sbjct: 114 ADMIKGKTPEEIRSTFNIENDFTPEEEEAVRKENQWAFE 152
>At1g10230.1 68414.m01153 E3 ubiquitin ligase SCF complex subunit
SKP1/ASK1 (At18), putative E3 ubiquitin ligase; similar
to Skp1 homolog Skp1a GI:3068807 [Arabidopsis thaliana]
Length = 183
Score = 57.6 bits (133), Expect = 2e-09
Identities = 23/36 (63%), Positives = 30/36 (83%)
Frame = +2
Query: 11 ANMIKGKTPEEIRKTFNIKNDFTAAEEDQVRKENEW 118
A+ IK KTPEE+R+ FNI+NDFT EE+++RKEN W
Sbjct: 144 ADYIKDKTPEEVREIFNIENDFTPEEEEEIRKENAW 179
>At2g03170.1 68415.m00270 E3 ubiquitin ligase SCF complex subunit
SKP1/ASK1 (At14), putative E3 ubiquitin ligase; similar
to Skp1 homolog Skp1b GI:3068809, UIP2 GI:3719211 from
[Arabidopsis thaliana]
Length = 149
Score = 56.4 bits (130), Expect = 5e-09
Identities = 26/39 (66%), Positives = 29/39 (74%)
Frame = +2
Query: 11 ANMIKGKTPEEIRKTFNIKNDFTAAEEDQVRKENEWCEE 127
A+ IK KTPEEIR+ FNI+NDFT EE VRKEN W E
Sbjct: 111 ADRIKDKTPEEIREIFNIENDFTPEEEAAVRKENAWAFE 149
>At1g20140.1 68414.m02519 E3 ubiquitin ligase SCF complex subunit,
putative similar to Skp1 GI:4959710 from [Medicago
sativa]
Length = 163
Score = 54.0 bits (124), Expect = 3e-08
Identities = 22/39 (56%), Positives = 30/39 (76%)
Frame = +2
Query: 11 ANMIKGKTPEEIRKTFNIKNDFTAAEEDQVRKENEWCEE 127
A+ ++GKTPE++R FNIKND+T EE +VR EN+W E
Sbjct: 125 ADQMRGKTPEQMRAHFNIKNDYTPEEEAEVRNENKWAFE 163
>At3g60010.1 68416.m06700 E3 ubiquitin ligase SCF complex subunit
SKP1/ASK1 (At13), putative E3 ubiquitin ligase; similar
to Skp1 homolog Skp1a GI:3068807 from [Arabidopsis
thaliana]
Length = 154
Score = 53.6 bits (123), Expect = 3e-08
Identities = 22/39 (56%), Positives = 29/39 (74%)
Frame = +2
Query: 11 ANMIKGKTPEEIRKTFNIKNDFTAAEEDQVRKENEWCEE 127
A+MI GK P+EIR I+NDFT EE+++RKEN+W E
Sbjct: 116 ADMITGKKPDEIRALLGIENDFTPEEEEEIRKENQWAFE 154
>At3g25650.1 68416.m03192 Skp1 family protein similar toSkp1
[Medicago sativa] GI:4959710, fimbriata-associated
protein [Antirrhinum majus] GI:2673870, UIP2
[Arabidopsis thaliana] GI:3719211; contains Pfam profile
PF01466: Skp1 family, dimerisation domain
Length = 177
Score = 53.6 bits (123), Expect = 3e-08
Identities = 24/40 (60%), Positives = 31/40 (77%), Gaps = 1/40 (2%)
Frame = +2
Query: 11 ANMIKGKTPEEIRKTFNIKNDFTAAEEDQ-VRKENEWCEE 127
A+ IK KTPEE+R+ FNI+NDFT EE++ +RKEN W E
Sbjct: 128 ADYIKDKTPEEVRELFNIENDFTHEEEEEAIRKENAWAFE 167
>At3g21850.1 68416.m02754 E3 ubiquitin ligase SCF complex subunit
SKP1/ASK1 (At9), putative E3 ubiquitin ligase; similar
to Skp1 homolog SKP1a GI:3068807 from [Arabidopsis
thaliana]
Length = 153
Score = 52.8 bits (121), Expect = 6e-08
Identities = 22/39 (56%), Positives = 29/39 (74%)
Frame = +2
Query: 11 ANMIKGKTPEEIRKTFNIKNDFTAAEEDQVRKENEWCEE 127
A +IKG TPE+IR+ FNI+ND T EE +R+EN+W E
Sbjct: 115 AEIIKGNTPEQIREFFNIENDLTPEEEAAIRRENKWAFE 153
>At2g03160.1 68415.m00269 E3 ubiquitin ligase SCF complex subunit
SKP1/ASK1 (At19), putative E3 ubiquitin ligase; similar
to Skp1 GI:4959710 from [Medicago sativa]
Length = 200
Score = 52.8 bits (121), Expect = 6e-08
Identities = 22/40 (55%), Positives = 29/40 (72%)
Frame = +2
Query: 11 ANMIKGKTPEEIRKTFNIKNDFTAAEEDQVRKENEWCEEK 130
A+ IK TPEE+R+ FNI+NDFT EE+ +R EN W E+
Sbjct: 152 ADYIKDMTPEEVRELFNIENDFTPEEEEAIRNENAWTFEQ 191
>At3g21830.1 68416.m02752 E3 ubiquitin ligase SCF complex subunit
SKP1/ASK1 (At8), putative E3 ubiquitin ligase; similar
to Skp1 homolog Skp1b GI:3068809, UIP2 GI:3719211 from
[Arabidopsis thaliana]
Length = 152
Score = 52.0 bits (119), Expect = 1e-07
Identities = 21/39 (53%), Positives = 30/39 (76%)
Frame = +2
Query: 11 ANMIKGKTPEEIRKTFNIKNDFTAAEEDQVRKENEWCEE 127
A+MIKG TP+++R+ FNI+ND T EE +R+EN+W E
Sbjct: 114 ADMIKGNTPKQMREFFNIENDLTPEEEAAIRRENKWAFE 152
>At2g25700.1 68415.m03080 E3 ubiquitin ligase SCF complex subunit
SKP1/ASK1 (At3), putative E3 ubiquitin ligase; similar
to fimbriata-associated protein fap1 GI:2673868 from
[Antirrhinum majus]
Length = 163
Score = 52.0 bits (119), Expect = 1e-07
Identities = 21/39 (53%), Positives = 29/39 (74%)
Frame = +2
Query: 11 ANMIKGKTPEEIRKTFNIKNDFTAAEEDQVRKENEWCEE 127
A+ ++GKTP ++R+ FNIKND+T EE +VR EN W E
Sbjct: 125 ADQMRGKTPAQMREHFNIKNDYTPEEEAEVRNENRWAFE 163
>At2g03190.1 68415.m00272 E3 ubiquitin ligase SCF complex subunit
SKP1/ASK1 (At16), putative E3 ubiquitin ligase; similar
to Skp1 homolog Skp1a GI:3068807 from [Arabidopsis
thaliana]
Length = 170
Score = 52.0 bits (119), Expect = 1e-07
Identities = 21/39 (53%), Positives = 29/39 (74%)
Frame = +2
Query: 11 ANMIKGKTPEEIRKTFNIKNDFTAAEEDQVRKENEWCEE 127
A+ +K +PEE+R+ FNI+ND+T EED +RKEN W E
Sbjct: 129 ADHMKDMSPEEVRELFNIENDYTPEEEDAIRKENAWAFE 167
>At3g60020.1 68416.m06702 E3 ubiquitin ligase SCF complex subunit
SKP1/ASK1 (At5), putative E3 ubiquitin ligase; similar
to Skp1 GI:4959710 from [Medicago sativa]
Length = 153
Score = 51.2 bits (117), Expect = 2e-07
Identities = 20/36 (55%), Positives = 29/36 (80%)
Frame = +2
Query: 11 ANMIKGKTPEEIRKTFNIKNDFTAAEEDQVRKENEW 118
A+++ GKTP+EIR FNI+ND TA E ++R+EN+W
Sbjct: 115 ADLLSGKTPDEIRAHFNIENDLTAEEVAKIREENQW 150
>At3g21860.1 68416.m02755 E3 ubiquitin ligase SCF complex subunit
SKP1/ASK1 (At10), putative E3 ubiquitin ligase; similar
to Skp1 homolog Skp1b GI:3068809, UIP2 GI:3719211 from
[Arabidopsis thaliana]
Length = 152
Score = 49.6 bits (113), Expect = 5e-07
Identities = 21/36 (58%), Positives = 27/36 (75%)
Frame = +2
Query: 11 ANMIKGKTPEEIRKTFNIKNDFTAAEEDQVRKENEW 118
A+MIK T E RK FNI+ND+T EE+ VR+EN+W
Sbjct: 114 ADMIKDNTVEHTRKFFNIENDYTHEEEEAVRRENQW 149
>At2g20160.1 68415.m02357 E3 ubiquitin ligase SCF complex subunit
SKP1/ASK1 (At17), putative E3 ubiquitin ligase; similar
to Skp1 homolog Skp1b GI:3068809, UIP2 GI:3719211 from
[Arabidopsis thaliana]
Length = 150
Score = 46.8 bits (106), Expect = 4e-06
Identities = 19/36 (52%), Positives = 27/36 (75%)
Frame = +2
Query: 11 ANMIKGKTPEEIRKTFNIKNDFTAAEEDQVRKENEW 118
A+ KT EIR+ FNI+ND+T EE+++RK+NEW
Sbjct: 112 ADYTADKTVNEIRELFNIENDYTPEEEEELRKKNEW 147
>At3g61415.1 68416.m06878 SKP1 family protein low similarity to
SP|P52285 Glycoprotein FP21 precursor {Dictyostelium
discoideum}; contains Pfam profile PF01466: Skp1 family,
dimerisation domain
Length = 351
Score = 34.7 bits (76), Expect = 0.017
Identities = 14/27 (51%), Positives = 21/27 (77%)
Frame = +2
Query: 11 ANMIKGKTPEEIRKTFNIKNDFTAAEE 91
A +I+GKTPEEIR+ F++ +D T E+
Sbjct: 128 ARIIEGKTPEEIREIFHLPDDLTEEEK 154
>At2g45950.1 68415.m05713 SKP1 family protein similar to
glycoprotein FP21 SP:P52285 from [Dictyostelium
discoideum]; contains Pfam profile PF01466: Skp1 family,
dimerisation domain
Length = 342
Score = 32.7 bits (71), Expect = 0.067
Identities = 13/27 (48%), Positives = 20/27 (74%)
Frame = +2
Query: 11 ANMIKGKTPEEIRKTFNIKNDFTAAEE 91
A +I+GK PEEIR+ F++ +D T E+
Sbjct: 128 ARIIEGKNPEEIREIFHLPDDLTEEEK 154
>At1g52970.1 68414.m05992 hypothetical protein
Length = 149
Score = 27.5 bits (58), Expect = 2.5
Identities = 10/19 (52%), Positives = 12/19 (63%)
Frame = +2
Query: 230 KPSKFVCVVNYIKIGTSCH 286
KP C VN++KIG CH
Sbjct: 84 KPVSEDCCVNFLKIGRECH 102
>At5g02820.1 68418.m00224 DNA topoisomerase VIA (SPO11) identical to
topoisomerase 6 subunit A (spo11) [Arabidopsis thaliana]
GI:12331186
Length = 427
Score = 25.8 bits (54), Expect = 7.7
Identities = 16/44 (36%), Positives = 22/44 (50%), Gaps = 3/44 (6%)
Frame = +2
Query: 5 RAANMIKGKTPEEIRKTF---NIKNDFTAAEEDQVRKENEWCEE 127
R +++ K K PE+ R +IK EED V+K W EE
Sbjct: 345 RPSDLDKYKIPEQCRLPMTEQDIKTGKDMLEEDFVKKNPGWVEE 388
>At4g27970.1 68417.m04013 C4-dicarboxylate transporter/malic acid
transport family protein contains Pfam profile PF03595:
C4-dicarboxylate transporter/malic acid transport
protein
Length = 519
Score = 25.8 bits (54), Expect = 7.7
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +3
Query: 150 FASIFMYYHYVCNIKLF 200
F S+F+Y+ VC I LF
Sbjct: 372 FISLFLYFSLVCRINLF 388
>At2g26260.1 68415.m03152 3-beta hydroxysteroid
dehydrogenase/isomerase family protein contains Pfam
profile PF01073 3-beta hydroxysteroid
dehydrogenase/isomerase domain; similar to
NAD(P)-dependent steroid dehydrogenase from Homo sapiens
[SP|Q15738], Mus musculus [SP|Q9R1J0]
Length = 564
Score = 25.8 bits (54), Expect = 7.7
Identities = 15/58 (25%), Positives = 30/58 (51%)
Frame = +3
Query: 27 ERLLKKSVKRSTLRMISLLQKKIRFVKRMNGVKKSKP**MLFASIFMYYHYVCNIKLF 200
+ LL K +K++ + + L+ FV + V + +L AS+F++ H + K+F
Sbjct: 386 DTLLWKDLKQTLIAIFILISIYYNFVATGSTVVTALSKALLVASVFLFLHGILPEKIF 443
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,195,918
Number of Sequences: 28952
Number of extensions: 110589
Number of successful extensions: 284
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 280
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 283
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 419412672
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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