Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0675
         (662 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g16340.1 68417.m02476 adapter protein SPIKE1 (SPK1) One model...    51   6e-07
At5g20310.1 68418.m02417 hypothetical protein                          30   1.6  
At4g19000.1 68417.m02798 IWS1 C-terminus family protein contains...    29   2.8  
At5g66490.1 68418.m08384 expressed protein                             28   6.4  
At1g64900.1 68414.m07357 cytochrome P450, putative similar to cy...    28   6.4  
At5g66570.1 68418.m08392 oxygen-evolving enhancer protein 1-1, c...    27   8.4  

>At4g16340.1 68417.m02476 adapter protein SPIKE1 (SPK1) One model
            reflects the alignment of a full-length cDNA sequence
            gi:18496702.  There are multiple frame shifts in the gene
            model resulting in a truncated protein.  The alternate
            model includes modifications in exons 14, 17 and 29 to
            compensate for frame shifts and maximize the protein
            length.  It is not based on EST data.  adapter protein
            SPIKE1 [Arabidopsis thaliana] GI:18496703
          Length = 1757

 Score = 51.2 bits (117), Expect = 6e-07
 Identities = 40/145 (27%), Positives = 66/145 (45%), Gaps = 7/145 (4%)
 Frame = +2

Query: 26   TAHHFPYVKTRIQVVQKTQIILSPIEVAIEDIQKKVA----ELSAATSAEPADA---KML 184
            T   FP +  R+ V +   +  SP+E AI  I+ +      EL    S++       + L
Sbjct: 1603 TEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTTALRNELEEPRSSDGDHLPRLQSL 1662

Query: 185  QMVLQGCVTTTVNQGPLELAQVFLAPVADGTQPPTRLTNKLRLTFKDFYKKCQDALKKNK 364
            Q +LQG V   VN G L +   FL+     T+  ++   +L     +F   C+ A++ + 
Sbjct: 1663 QRILQGSVAVQVNSGVLSVCTAFLSG-EPATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1721

Query: 365  NLIGLDQREYQRELERNLQRLAASL 439
             LIG + +E+  +L    Q L A L
Sbjct: 1722 RLIGEEDQEFHTQLVNGFQSLTAEL 1746


>At5g20310.1 68418.m02417 hypothetical protein
          Length = 394

 Score = 29.9 bits (64), Expect = 1.6
 Identities = 15/51 (29%), Positives = 27/51 (52%)
 Frame = +2

Query: 254 LAPVADGTQPPTRLTNKLRLTFKDFYKKCQDALKKNKNLIGLDQREYQREL 406
           L+PV D T    RL  +L+ T   ++  C++AL + +  + L   + + EL
Sbjct: 276 LSPVDDATAEMRRLKKELKETMNMYHAACKEALMEKERAVELVMWKKKAEL 326


>At4g19000.1 68417.m02798 IWS1 C-terminus family protein contains
           Pfam profile PF05909: IWS1 C-terminus
          Length = 406

 Score = 29.1 bits (62), Expect = 2.8
 Identities = 20/100 (20%), Positives = 49/100 (49%), Gaps = 7/100 (7%)
 Frame = +2

Query: 50  KTRIQVVQKTQIILSPIEVAIEDIQKKVAELSAATSAEPADAKMLQMVLQGCVTTT---- 217
           KT +++  + + +++ +E+A+ED      ++      +PA  K++++ L     +     
Sbjct: 138 KTSMEIGMQVEQVMANLEIAVED------DVICNREGKPAINKLMKLPLLNETLSKKPLQ 191

Query: 218 ---VNQGPLELAQVFLAPVADGTQPPTRLTNKLRLTFKDF 328
              ++ G L L + +L P+ DG+ P   + + + +   DF
Sbjct: 192 GEFLDHGVLNLLKNWLEPLPDGSLPNINIRSAVLMILNDF 231


>At5g66490.1 68418.m08384 expressed protein
          Length = 109

 Score = 27.9 bits (59), Expect = 6.4
 Identities = 16/55 (29%), Positives = 28/55 (50%)
 Frame = +2

Query: 68  VQKTQIILSPIEVAIEDIQKKVAELSAATSAEPADAKMLQMVLQGCVTTTVNQGP 232
           V+KT+    P+    E   K+  +  + T+A+P  A+ L+ V +G V    + GP
Sbjct: 50  VEKTRGSAHPLFSFFEMSLKRKKKKKSTTTAKPEFARYLEYVKEGGVWDNTSNGP 104


>At1g64900.1 68414.m07357 cytochrome P450, putative similar to
           cytochrome p450 GI:438240 from [Solanum melongena]
          Length = 506

 Score = 27.9 bits (59), Expect = 6.4
 Identities = 16/52 (30%), Positives = 29/52 (55%), Gaps = 2/52 (3%)
 Frame = +2

Query: 329 YKKCQDALKKN-KNLIGLDQREYQRE-LERNLQRLAASLQPLLQHPPSHAAL 478
           Y + Q+ L +  K+++G + +E + E +E+     A  L+ L +HPP H  L
Sbjct: 325 YPEIQERLHEEIKSVVGEEAKEVEEEDVEKMPYLKAVVLEGLRRHPPGHFLL 376


>At5g66570.1 68418.m08392 oxygen-evolving enhancer protein 1-1,
           chloroplast / 33 kDa subunit of oxygen evolving system
           of photosystem II (PSBO1) (PSBO) identical to SP:P23321
           Oxygen-evolving enhancer protein 1-1, chloroplast
           precursor (OEE1) (33 kDa subunit of oxygen evolving
           system of photosystem II) (OEC 33 kDa subunit) (33 kDa
           thylakoid membrane protein) [Arabidopsis thaliana]
          Length = 332

 Score = 27.5 bits (58), Expect = 8.4
 Identities = 12/22 (54%), Positives = 14/22 (63%)
 Frame = +2

Query: 290 RLTNKLRLTFKDFYKKCQDALK 355
           RLT   +  FKDF  KC DA+K
Sbjct: 46  RLTCSFQSDFKDFTGKCSDAVK 67


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,424,983
Number of Sequences: 28952
Number of extensions: 166774
Number of successful extensions: 752
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 720
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 750
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)