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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0667
         (423 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g22080.1 68414.m02761 hypothetical protein                          31   0.43 
At2g17036.1 68415.m01966 F-box family protein  ; similar to SKP1...    29   1.7  
At1g21400.1 68414.m02678 2-oxoisovalerate dehydrogenase, putativ...    29   1.7  
At1g69540.1 68414.m07996 MADS-box family protein contains Pfam p...    27   5.2  
At1g18880.1 68414.m02350 proton-dependent oligopeptide transport...    27   6.9  
At1g03310.2 68414.m00310 isoamylase, putative / starch debranchi...    27   6.9  
At1g03310.1 68414.m00309 isoamylase, putative / starch debranchi...    27   6.9  
At5g65900.1 68418.m08295 DEAD/DEAH box helicase, putative contai...    26   9.1  

>At1g22080.1 68414.m02761 hypothetical protein
          Length = 475

 Score = 30.7 bits (66), Expect = 0.43
 Identities = 14/28 (50%), Positives = 17/28 (60%)
 Frame = -2

Query: 344 TARRSVFIRVDVGRFAFDCWKTSRCIFS 261
           TARR  F   D GR AF CW+ +R  F+
Sbjct: 53  TARRGPFHLSDDGRVAFLCWRVARPAFA 80


>At2g17036.1 68415.m01966 F-box family protein  ; similar to SKP1
           interacting partner 2 (SKIP2) TIGR_Ath1:At5g67250
          Length = 428

 Score = 28.7 bits (61), Expect = 1.7
 Identities = 18/51 (35%), Positives = 29/51 (56%)
 Frame = +3

Query: 3   KTEYLSKVPDPIALPFPESLYLQTYVKMAKVQVLYHTFGPGRKVLKTLPEK 155
           KTE ++K+P+   L +P   ++ T  +++  Q L H   P R VLK + EK
Sbjct: 362 KTELVNKLPEYAKLFWPPPPWITTSHEVSGFQSLNH---PNRVVLKIILEK 409


>At1g21400.1 68414.m02678 2-oxoisovalerate dehydrogenase, putative /
           3-methyl-2-oxobutanoate dehydrogenase, putative /
           branched-chain alpha-keto acid dehydrogenase E1 alpha
           subunit, putative similar to branched-chain alpha-keto
           acid dehydrogenase E1-alpha subunit [Gallus gallus]
           GI:12964598; contains Pfam profile PF00676:
           Dehydrogenase E1 component
          Length = 472

 Score = 28.7 bits (61), Expect = 1.7
 Identities = 12/31 (38%), Positives = 19/31 (61%)
 Frame = +3

Query: 24  VPDPIALPFPESLYLQTYVKMAKVQVLYHTF 116
           +PD   +P  E L ++ Y +MA +QV+ H F
Sbjct: 117 IPDSDFIPVSEKLAVRMYEQMATLQVMDHIF 147


>At1g69540.1 68414.m07996 MADS-box family protein contains Pfam
           profile: PF00319: SRF-type transcription factor
           (DNA-binding and dimerisation domain)
          Length = 378

 Score = 27.1 bits (57), Expect = 5.2
 Identities = 12/44 (27%), Positives = 19/44 (43%)
 Frame = -2

Query: 263 STSIFVYCSIFSDNNLHFIFLIECRPLLFHFFSNYLFFWQCF*D 132
           S+    Y ++F D      F +     + H FS   ++W CF D
Sbjct: 336 SSETLPYVAVFDDP---LYFWVNNGLFIIHLFSKLCYYWSCFAD 376


>At1g18880.1 68414.m02350 proton-dependent oligopeptide transport
           (POT) family protein contains Pfam profile: PF00854 POT
           family
          Length = 587

 Score = 26.6 bits (56), Expect = 6.9
 Identities = 11/23 (47%), Positives = 18/23 (78%)
 Frame = -1

Query: 156 VFLAMFLRLYVPVQMYDRVLVPW 88
           VFL + + +++P+  YDRVLVP+
Sbjct: 375 VFLMLGMTIFIPI--YDRVLVPF 395


>At1g03310.2 68414.m00310 isoamylase, putative / starch debranching
           enzyme, putative similar to isoamylase isoform 2
           [Solanum tuberosum] GI:27728147, isoamylase from
           [Triticum aestivum] GI:17932898, [Hordeum vulgare]
           GI:21314275, [Oryza sativa] GI:3252794; contains Pfam
           profiles PF00128: Alpha amylase catalytic domain,
           PF02922: Isoamylase N-terminal domain; ESTs gb|H36690,
           gb|AA712462, gb|AA651230 and gb|N95932 come from this
           gene
          Length = 882

 Score = 26.6 bits (56), Expect = 6.9
 Identities = 12/35 (34%), Positives = 17/35 (48%)
 Frame = +3

Query: 183 QRSTFYKKYKMKIVVGEDGTVHEYGCAKDTPRCFP 287
           + S FY  + +K+V G D    E    +DT  C P
Sbjct: 190 KESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIP 224


>At1g03310.1 68414.m00309 isoamylase, putative / starch debranching
           enzyme, putative similar to isoamylase isoform 2
           [Solanum tuberosum] GI:27728147, isoamylase from
           [Triticum aestivum] GI:17932898, [Hordeum vulgare]
           GI:21314275, [Oryza sativa] GI:3252794; contains Pfam
           profiles PF00128: Alpha amylase catalytic domain,
           PF02922: Isoamylase N-terminal domain; ESTs gb|H36690,
           gb|AA712462, gb|AA651230 and gb|N95932 come from this
           gene
          Length = 882

 Score = 26.6 bits (56), Expect = 6.9
 Identities = 12/35 (34%), Positives = 17/35 (48%)
 Frame = +3

Query: 183 QRSTFYKKYKMKIVVGEDGTVHEYGCAKDTPRCFP 287
           + S FY  + +K+V G D    E    +DT  C P
Sbjct: 190 KESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIP 224


>At5g65900.1 68418.m08295 DEAD/DEAH box helicase, putative contains
           Pfam profiles  PF00270:DEAD/DEAH box helicase and
           PF00271:  Helicase conserved C-terminal domain;
           identical to cDNA RH27 helicase, partial GI:4033334
          Length = 633

 Score = 26.2 bits (55), Expect = 9.1
 Identities = 18/56 (32%), Positives = 28/56 (50%), Gaps = 3/56 (5%)
 Frame = +3

Query: 51  PESLYLQTYVKMAKVQVLYHTFGPGRKVLKTLP-EKQIVAEK--VKEQRSTFYKKY 209
           P+ L    Y+K AK+ V  H F   +K+L   P  + +++E   +KE     YK Y
Sbjct: 507 PQELKFIQYLKAAKIPVEEHEF-EEKKLLDVKPFVENLISENYALKESAKEAYKTY 561


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,995,301
Number of Sequences: 28952
Number of extensions: 216399
Number of successful extensions: 684
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 674
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 684
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 655255392
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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