BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0667 (423 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g22080.1 68414.m02761 hypothetical protein 31 0.43 At2g17036.1 68415.m01966 F-box family protein ; similar to SKP1... 29 1.7 At1g21400.1 68414.m02678 2-oxoisovalerate dehydrogenase, putativ... 29 1.7 At1g69540.1 68414.m07996 MADS-box family protein contains Pfam p... 27 5.2 At1g18880.1 68414.m02350 proton-dependent oligopeptide transport... 27 6.9 At1g03310.2 68414.m00310 isoamylase, putative / starch debranchi... 27 6.9 At1g03310.1 68414.m00309 isoamylase, putative / starch debranchi... 27 6.9 At5g65900.1 68418.m08295 DEAD/DEAH box helicase, putative contai... 26 9.1 >At1g22080.1 68414.m02761 hypothetical protein Length = 475 Score = 30.7 bits (66), Expect = 0.43 Identities = 14/28 (50%), Positives = 17/28 (60%) Frame = -2 Query: 344 TARRSVFIRVDVGRFAFDCWKTSRCIFS 261 TARR F D GR AF CW+ +R F+ Sbjct: 53 TARRGPFHLSDDGRVAFLCWRVARPAFA 80 >At2g17036.1 68415.m01966 F-box family protein ; similar to SKP1 interacting partner 2 (SKIP2) TIGR_Ath1:At5g67250 Length = 428 Score = 28.7 bits (61), Expect = 1.7 Identities = 18/51 (35%), Positives = 29/51 (56%) Frame = +3 Query: 3 KTEYLSKVPDPIALPFPESLYLQTYVKMAKVQVLYHTFGPGRKVLKTLPEK 155 KTE ++K+P+ L +P ++ T +++ Q L H P R VLK + EK Sbjct: 362 KTELVNKLPEYAKLFWPPPPWITTSHEVSGFQSLNH---PNRVVLKIILEK 409 >At1g21400.1 68414.m02678 2-oxoisovalerate dehydrogenase, putative / 3-methyl-2-oxobutanoate dehydrogenase, putative / branched-chain alpha-keto acid dehydrogenase E1 alpha subunit, putative similar to branched-chain alpha-keto acid dehydrogenase E1-alpha subunit [Gallus gallus] GI:12964598; contains Pfam profile PF00676: Dehydrogenase E1 component Length = 472 Score = 28.7 bits (61), Expect = 1.7 Identities = 12/31 (38%), Positives = 19/31 (61%) Frame = +3 Query: 24 VPDPIALPFPESLYLQTYVKMAKVQVLYHTF 116 +PD +P E L ++ Y +MA +QV+ H F Sbjct: 117 IPDSDFIPVSEKLAVRMYEQMATLQVMDHIF 147 >At1g69540.1 68414.m07996 MADS-box family protein contains Pfam profile: PF00319: SRF-type transcription factor (DNA-binding and dimerisation domain) Length = 378 Score = 27.1 bits (57), Expect = 5.2 Identities = 12/44 (27%), Positives = 19/44 (43%) Frame = -2 Query: 263 STSIFVYCSIFSDNNLHFIFLIECRPLLFHFFSNYLFFWQCF*D 132 S+ Y ++F D F + + H FS ++W CF D Sbjct: 336 SSETLPYVAVFDDP---LYFWVNNGLFIIHLFSKLCYYWSCFAD 376 >At1g18880.1 68414.m02350 proton-dependent oligopeptide transport (POT) family protein contains Pfam profile: PF00854 POT family Length = 587 Score = 26.6 bits (56), Expect = 6.9 Identities = 11/23 (47%), Positives = 18/23 (78%) Frame = -1 Query: 156 VFLAMFLRLYVPVQMYDRVLVPW 88 VFL + + +++P+ YDRVLVP+ Sbjct: 375 VFLMLGMTIFIPI--YDRVLVPF 395 >At1g03310.2 68414.m00310 isoamylase, putative / starch debranching enzyme, putative similar to isoamylase isoform 2 [Solanum tuberosum] GI:27728147, isoamylase from [Triticum aestivum] GI:17932898, [Hordeum vulgare] GI:21314275, [Oryza sativa] GI:3252794; contains Pfam profiles PF00128: Alpha amylase catalytic domain, PF02922: Isoamylase N-terminal domain; ESTs gb|H36690, gb|AA712462, gb|AA651230 and gb|N95932 come from this gene Length = 882 Score = 26.6 bits (56), Expect = 6.9 Identities = 12/35 (34%), Positives = 17/35 (48%) Frame = +3 Query: 183 QRSTFYKKYKMKIVVGEDGTVHEYGCAKDTPRCFP 287 + S FY + +K+V G D E +DT C P Sbjct: 190 KESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIP 224 >At1g03310.1 68414.m00309 isoamylase, putative / starch debranching enzyme, putative similar to isoamylase isoform 2 [Solanum tuberosum] GI:27728147, isoamylase from [Triticum aestivum] GI:17932898, [Hordeum vulgare] GI:21314275, [Oryza sativa] GI:3252794; contains Pfam profiles PF00128: Alpha amylase catalytic domain, PF02922: Isoamylase N-terminal domain; ESTs gb|H36690, gb|AA712462, gb|AA651230 and gb|N95932 come from this gene Length = 882 Score = 26.6 bits (56), Expect = 6.9 Identities = 12/35 (34%), Positives = 17/35 (48%) Frame = +3 Query: 183 QRSTFYKKYKMKIVVGEDGTVHEYGCAKDTPRCFP 287 + S FY + +K+V G D E +DT C P Sbjct: 190 KESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIP 224 >At5g65900.1 68418.m08295 DEAD/DEAH box helicase, putative contains Pfam profiles PF00270:DEAD/DEAH box helicase and PF00271: Helicase conserved C-terminal domain; identical to cDNA RH27 helicase, partial GI:4033334 Length = 633 Score = 26.2 bits (55), Expect = 9.1 Identities = 18/56 (32%), Positives = 28/56 (50%), Gaps = 3/56 (5%) Frame = +3 Query: 51 PESLYLQTYVKMAKVQVLYHTFGPGRKVLKTLP-EKQIVAEK--VKEQRSTFYKKY 209 P+ L Y+K AK+ V H F +K+L P + +++E +KE YK Y Sbjct: 507 PQELKFIQYLKAAKIPVEEHEF-EEKKLLDVKPFVENLISENYALKESAKEAYKTY 561 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,995,301 Number of Sequences: 28952 Number of extensions: 216399 Number of successful extensions: 684 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 674 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 684 length of database: 12,070,560 effective HSP length: 74 effective length of database: 9,928,112 effective search space used: 655255392 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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