Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0655
         (664 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef...   197   1e-51
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef...   197   1e-51
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef...   197   1e-51
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa...    73   3e-14
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote...    36   0.004
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein...    26   5.6  
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos...    25   7.4  
SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe...    25   9.7  
SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2...    25   9.7  
SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc...    25   9.7  

>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
           Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 460

 Score =  197 bits (480), Expect = 1e-51
 Identities = 88/115 (76%), Positives = 101/115 (87%)
 Frame = +1

Query: 4   GDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKS 183
           GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK 
Sbjct: 325 GDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKK 384

Query: 184 TEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 348
            E +PK +KSGDA I  +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 385 IEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439


>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
           Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 460

 Score =  197 bits (480), Expect = 1e-51
 Identities = 88/115 (76%), Positives = 101/115 (87%)
 Frame = +1

Query: 4   GDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKS 183
           GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK 
Sbjct: 325 GDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKK 384

Query: 184 TEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 348
            E +PK +KSGDA I  +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 385 IEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439


>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
           Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 460

 Score =  197 bits (480), Expect = 1e-51
 Identities = 88/115 (76%), Positives = 101/115 (87%)
 Frame = +1

Query: 4   GDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKS 183
           GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK 
Sbjct: 325 GDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKK 384

Query: 184 TEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 348
            E +PK +KSGDA I  +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 385 IEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439


>SPCC584.04 |sup35|erf3|translation release factor eRF3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 73.3 bits (172), Expect = 3e-14
 Identities = 39/115 (33%), Positives = 62/115 (53%), Gaps = 1/115 (0%)
 Frame = +1

Query: 10  SKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTE 189
           S  NP      F AQ+ +L  P  ++ GY+ V+  HTA     FA++  K+D+ T + ++
Sbjct: 549 STKNPVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLDK-TNRKSK 607

Query: 190 VNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKAVN 351
             P     G   I  L    P+C+E F+++  +GRF +RD   TVAVG V+K ++
Sbjct: 608 KPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662


>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 592

 Score = 36.3 bits (80), Expect = 0.004
 Identities = 32/108 (29%), Positives = 47/108 (43%)
 Frame = +1

Query: 19  NPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNP 198
           NP +    F A++   +  G I +G T VL     H+      +  K+     K +  + 
Sbjct: 490 NPVRRVRSFVAEIQTFDIHGPILSGSTLVL-----HLGRTVTSVSLKIVTVNNKRSR-HI 543

Query: 199 KSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 342
            S K     I  L    PLC+   +E P LGRF +R    TVA G++K
Sbjct: 544 ASRKRALVRISFLDGLFPLCLA--EECPALGRFILRRSGDTVAAGIVK 589


>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 670

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
 Frame = -3

Query: 392 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 219
           S  +VTLPPPAS   ++  T T T  + S ++     G+ + +++        +  ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241

Query: 218 SPDLMDFGLTSVDLPVRRSTFSLIS 144
           S  L    +TS   PV  S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266


>SPAC631.01c |acp2||F-actin capping protein beta subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 268

 Score = 25.4 bits (53), Expect = 7.4
 Identities = 11/25 (44%), Positives = 18/25 (72%)
 Frame = -3

Query: 227 MAASPDLMDFGLTSVDLPVRRSTFS 153
           ++ +PDL D  L+SVD P++ +T S
Sbjct: 27  LSVAPDLADVLLSSVDQPLKVNTCS 51


>SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 972

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 13/32 (40%), Positives = 14/32 (43%)
 Frame = +3

Query: 474 GVSKEKRATNSFLFYIFYKACNVTLFYNLYKV 569
           G S E     S+   IFY       FYNL KV
Sbjct: 762 GASSEMECYFSYCSLIFYYQATTLQFYNLPKV 793


>SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 244

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 10/37 (27%), Positives = 19/37 (51%)
 Frame = -3

Query: 407 PLVAFSAALVTLPPPASLKLTALMTPTATVCLMSRTA 297
           P  + ++   T+PP  S+  T  + PT ++   + TA
Sbjct: 73  PSTSHNSTTTTVPPTTSMNTTTTVPPTTSLNTTTTTA 109


>SPBC18H10.04c |sce3|tif48|translation initiation factor
           eIF4B|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 388

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 13/41 (31%), Positives = 20/41 (48%)
 Frame = -3

Query: 380 VTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDST 258
           + L P +S  +    TP+AT    S+  + P GG    D+T
Sbjct: 244 LNLKPRSSSNVNTEATPSATTTTSSKPKRDPFGGAKPVDNT 284


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,599,861
Number of Sequences: 5004
Number of extensions: 51295
Number of successful extensions: 150
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 149
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 301829700
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)