Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0655
(664 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 180 6e-46
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 180 6e-46
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 180 6e-46
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 180 6e-46
At1g35550.1 68414.m04414 elongation factor Tu C-terminal domain-... 141 4e-34
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 69 3e-12
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 52 5e-07
At4g27900.2 68417.m04005 expressed protein 32 0.39
At4g27900.1 68417.m04004 expressed protein 32 0.39
At1g55420.1 68414.m06339 DC1 domain-containing protein contains ... 29 3.6
At4g25650.1 68417.m03693 Rieske [2Fe-2S] domain-containing prote... 28 4.8
At2g04740.1 68415.m00484 ankyrin repeat family protein contains ... 28 6.4
At1g55380.1 68414.m06334 DC1 domain-containing protein contains ... 28 6.4
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 180 bits (439), Expect = 6e-46
Identities = 79/117 (67%), Positives = 100/117 (85%)
Frame = +1
Query: 1 AGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGK 180
A +SK++P KGAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+GK
Sbjct: 314 ASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGK 373
Query: 181 STEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 351
E PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+
Sbjct: 374 EIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 180 bits (439), Expect = 6e-46
Identities = 79/117 (67%), Positives = 100/117 (85%)
Frame = +1
Query: 1 AGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGK 180
A +SK++P KGAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+GK
Sbjct: 314 ASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGK 373
Query: 181 STEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 351
E PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+
Sbjct: 374 EIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 180 bits (439), Expect = 6e-46
Identities = 79/117 (67%), Positives = 100/117 (85%)
Frame = +1
Query: 1 AGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGK 180
A +SK++P KGAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+GK
Sbjct: 314 ASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGK 373
Query: 181 STEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 351
E PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+
Sbjct: 374 EIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 180 bits (439), Expect = 6e-46
Identities = 79/117 (67%), Positives = 100/117 (85%)
Frame = +1
Query: 1 AGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGK 180
A +SK++P KGAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+GK
Sbjct: 314 ASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGK 373
Query: 181 STEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 351
E PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+
Sbjct: 374 EIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430
>At1g35550.1 68414.m04414 elongation factor Tu C-terminal
domain-containing protein similar to SP|P13905
Elongation factor 1-alpha (EF-1-alpha) {Arabidopsis
thaliana}; contains Pfam profile PF03143: Elongation
factor Tu C-terminal domain
Length = 104
Score = 141 bits (341), Expect = 4e-34
Identities = 61/95 (64%), Positives = 77/95 (81%)
Frame = +1
Query: 64 LNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVP 243
+NH GQI NGYTPVLDCHT+HIA KF+EI K+D RTG E PK +K+ +AAI+N+ P
Sbjct: 1 MNHLGQIKNGYTPVLDCHTSHIAVKFSEILTKIDWRTGHEIEKEPKFLKNSEAAIINMTP 60
Query: 244 SKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 348
+KP+ VE++ +PPLGRFA+RDMRQTV VGVIK+V
Sbjct: 61 TKPMVVEAYSAYPPLGRFAIRDMRQTVGVGVIKSV 95
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 68.9 bits (161), Expect = 3e-12
Identities = 33/109 (30%), Positives = 56/109 (51%), Gaps = 2/109 (1%)
Frame = +1
Query: 19 NPPKGAADFTAQVIVLN--HPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEV 192
NP +F AQ+ +L + GY +L H C+ E+K ++D +T K +
Sbjct: 414 NPVPAVTEFVAQLQILELLDNAIFTAGYKAILHIHAVVEECEIIELKSQIDLKTRKPMKK 473
Query: 193 NPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVI 339
+K+G A + + + +C+E F +FP LGRF +R +T+AVG +
Sbjct: 474 KVLFVKNGAAVVCRIQVTNSICIEKFSDFPQLGRFTLRTEGKTIAVGKV 522
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 51.6 bits (118), Expect = 5e-07
Identities = 30/109 (27%), Positives = 52/109 (47%)
Frame = +1
Query: 22 PPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPK 201
P A V+VL I G H A A ++ +D +TG+ T+ +P+
Sbjct: 555 PVSVATHLELMVLVLEGATPILLGSQLEFHVHHAKEAATVVKLVAMLDPKTGQPTKKSPR 614
Query: 202 SIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 348
+ + +A++ + P+CVE+F E LGR +R +TVA+G + +
Sbjct: 615 CLTAKQSAMLEVSLQNPVCVETFSESRALGRVFLRSSGRTVAMGKVTRI 663
>At4g27900.2 68417.m04005 expressed protein
Length = 261
Score = 31.9 bits (69), Expect = 0.39
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = +1
Query: 196 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 288
PKS+ SG+ + +++V K ++ F +FPP+
Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174
>At4g27900.1 68417.m04004 expressed protein
Length = 261
Score = 31.9 bits (69), Expect = 0.39
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = +1
Query: 196 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 288
PKS+ SG+ + +++V K ++ F +FPP+
Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174
>At1g55420.1 68414.m06339 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 725
Score = 28.7 bits (61), Expect = 3.6
Identities = 15/38 (39%), Positives = 21/38 (55%), Gaps = 5/38 (13%)
Frame = +3
Query: 519 IFYKACN---VTLFYNLYKVIHNI--SETFCYDCKLKC 617
I+ K C+ V LFY ++ N S FCY C+L+C
Sbjct: 619 IYLKPCHIFKVGLFYKEVEIARNDGNSRLFCYICRLRC 656
>At4g25650.1 68417.m03693 Rieske [2Fe-2S] domain-containing protein
similar to cell death suppressor protein lls1 from Zea
mays [gi:1935909], Rieske iron-sulfur protein Tic55 from
Pisum sativum [gi:2764524]; contains Pfam PF00355 Rieske
[2Fe-2S] domain
Length = 536
Score = 28.3 bits (60), Expect = 4.8
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +1
Query: 136 KFAEIKEKVDRRTGKSTEVNPKSI 207
+F + KEK+DR GK E+N K +
Sbjct: 258 RFPKPKEKIDREGGKPLEINVKKL 281
>At2g04740.1 68415.m00484 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 578
Score = 27.9 bits (59), Expect = 6.4
Identities = 19/67 (28%), Positives = 31/67 (46%), Gaps = 1/67 (1%)
Frame = -3
Query: 503 VCSPFFLRNTFR*MKYRS-CMKNCAVNSSSYFLPLVAFSAALVTLPPPASLKLTALMTPT 327
+C L + + +K R C+ A N + F+ F A L+TLPPP+ P+
Sbjct: 453 LCQWLVLSDMYGVLKIREYCLDLVACNFEA-FVETHEFRAMLLTLPPPSGDSSLRTTVPS 511
Query: 326 ATVCLMS 306
A +M+
Sbjct: 512 APGAMMT 518
>At1g55380.1 68414.m06334 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 661
Score = 27.9 bits (59), Expect = 6.4
Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 5/38 (13%)
Frame = +3
Query: 519 IFYKACN---VTLFYNLYKVIHNI--SETFCYDCKLKC 617
I+ K C+ V L+Y ++ N S FCY C+L+C
Sbjct: 579 IYLKPCHIFKVGLYYKEVEIARNDGNSRLFCYTCELRC 616
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,673,743
Number of Sequences: 28952
Number of extensions: 273333
Number of successful extensions: 779
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 755
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 779
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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