Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0646
(600 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g08310.1 68417.m01372 expressed protein glutamic acid-rich pr... 33 0.14
At5g17930.1 68418.m02102 MA3 domain-containing protein low simil... 32 0.33
At4g19230.2 68417.m02837 cytochrome P450 family protein cytochro... 31 0.44
At4g02710.1 68417.m00366 kinase interacting family protein simil... 31 0.77
At5g17240.1 68418.m02020 SET domain-containing protein contains ... 29 2.4
At5g31412.1 68418.m03722 hypothetical protein contains Pfam doma... 29 3.1
At2g07240.1 68415.m00831 Ulp1 protease family protein contains P... 29 3.1
At1g66130.1 68414.m07505 oxidoreductase N-terminal domain-contai... 28 4.1
At5g64710.2 68418.m08135 expressed protein contains Pfam profile... 28 5.4
At5g64710.1 68418.m08134 expressed protein contains Pfam profile... 28 5.4
At3g04810.2 68416.m00521 protein kinase, putative similar to LST... 28 5.4
At3g04810.1 68416.m00520 protein kinase, putative similar to LST... 28 5.4
At1g33670.1 68414.m04165 leucine-rich repeat family protein cont... 28 5.4
At5g60620.1 68418.m07608 phospholipid/glycerol acyltransferase f... 27 7.2
At3g20860.1 68416.m02637 protein kinase family protein contains ... 27 7.2
At2g04230.1 68415.m00410 F-box family protein contains F-box dom... 27 7.2
>At4g08310.1 68417.m01372 expressed protein glutamic acid-rich
protein precursor - Plasmodium falciparum, PIR2:A54514
Length = 504
Score = 33.1 bits (72), Expect = 0.14
Identities = 22/59 (37%), Positives = 30/59 (50%)
Frame = +1
Query: 46 DEQKENLKKHRADCLAETKADEQLVNKLKTGDFKTENEPLKKYALCMLIKSQLMTKDGK 222
D K +K+H CLA + DE N L+T + K + P+K+ A L K KDGK
Sbjct: 77 DVHKSFVKQHLVQCLAGAENDETSENSLET-EKKDDVTPVKEAA--ELSKEHTTKKDGK 132
>At5g17930.1 68418.m02102 MA3 domain-containing protein low
similarity to SP|Q9P6R9 Cell cycle control protein cwf22
{Schizosaccharomyces pombe}; contains Pfam profile
PF02847: MA3 domain
Length = 707
Score = 31.9 bits (69), Expect = 0.33
Identities = 26/104 (25%), Positives = 47/104 (45%), Gaps = 2/104 (1%)
Frame = +1
Query: 79 ADCLAETKADE--QLVNKLKTGDFKTENEPLKKYALCMLIKSQLMTKDGKFKKDVALAKV 252
A+ +AET E + LK + + N +K C+++ S+ F+K + L +
Sbjct: 479 AEDIAETMDAEVVEAQKMLKLAEAQRMNTDSRKAIFCVIMSSEDYID--AFEKLLRL-DL 535
Query: 253 PNAEDKLKVEKLIDACLANKGNSPHQTAWNYVKCYHEKDPKHAL 384
P +D+ + L++ CL K + T C H+K+ K L
Sbjct: 536 PGKQDREIMRVLVECCLQEKAFNKFYTVLASKLCEHDKNHKFTL 579
>At4g19230.2 68417.m02837 cytochrome P450 family protein cytochrome
P450, Arabidopsis thaliana; supported by cDNA:
gi_15293092_gb_AY050980.1_
Length = 484
Score = 31.5 bits (68), Expect = 0.44
Identities = 16/32 (50%), Positives = 19/32 (59%)
Frame = -3
Query: 382 ERASGLSRGSISRNSRLFGEGCCLCWPGKRQS 287
ERA+G S G +SR C LCWPG R+S
Sbjct: 439 ERATGFSMG----HSRFPKTDCPLCWPGSRRS 466
>At4g02710.1 68417.m00366 kinase interacting family protein similar
to kinase interacting protein 1 (GI:13936326) [Petunia
integrifolia]
Length = 1111
Score = 30.7 bits (66), Expect = 0.77
Identities = 18/52 (34%), Positives = 28/52 (53%), Gaps = 1/52 (1%)
Frame = -3
Query: 463 LDKHVRASYLTLMRYGTVGL-DYVFTRKERASGLSRGSISRNSRLFGEGCCL 311
++K R+ + L+ L DYVFT +E A +S+G+ + L EG CL
Sbjct: 760 IEKLKRSQTIVLLNESIKSLEDYVFTHRESAGEVSKGADLMDEFLKLEGMCL 811
>At5g17240.1 68418.m02020 SET domain-containing protein contains
Pfam profile PF00856: SET domain
Length = 491
Score = 29.1 bits (62), Expect = 2.4
Identities = 17/67 (25%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
Frame = +1
Query: 163 LKKYALCMLI-KSQLMTKDGKFKKDVALAKVPNAEDKLKVEKLIDACLANKGNSPHQTAW 339
LKK L + + + LMT + KD+ L+ N + L +++ CL + + ++ W
Sbjct: 57 LKKGELVLKVPRKALMTTESIIAKDLKLSDAVNLHNSLSSTQILSVCLLYEMSKEKKSFW 116
Query: 340 NYVKCYH 360
Y +H
Sbjct: 117 -YPYLFH 122
>At5g31412.1 68418.m03722 hypothetical protein contains Pfam domain,
PF04937: Protein of unknown function (DUF 659)
Length = 433
Score = 28.7 bits (61), Expect = 3.1
Identities = 20/64 (31%), Positives = 31/64 (48%), Gaps = 2/64 (3%)
Frame = +1
Query: 73 HRADCLAETKADEQLVNKLKT-GDFKTENEPLKKYALCMLIKSQLMTKD-GKFKKDVALA 246
H D + E + + K++T GD+K EN L +Y C+L + +D G K + LA
Sbjct: 227 HTVDLMLEAISKLPKMGKMQTFGDYKGENLFLNRYESCVLEWCDVCVEDFGPLMKLLRLA 286
Query: 247 KVPN 258
N
Sbjct: 287 DGEN 290
>At2g07240.1 68415.m00831 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain
Length = 928
Score = 28.7 bits (61), Expect = 3.1
Identities = 17/46 (36%), Positives = 27/46 (58%), Gaps = 2/46 (4%)
Frame = -1
Query: 321 AVAFVGQASVNQLLYFQFVFSIRHFSQS--DVLLEFSVLGHQLRFD 190
+++ VG+AS++ F + +HFS DVL++FS H LR D
Sbjct: 691 SISIVGEASLSHKEIFDIIERKKHFSSKVMDVLIKFS--RHLLRTD 734
>At1g66130.1 68414.m07505 oxidoreductase N-terminal
domain-containing protein similar to AX110P [Daucus
carota] GI:285739; contains Pfam profile PF01408:
Oxidoreductase family NAD-binding Rossmann fold
Length = 364
Score = 28.3 bits (60), Expect = 4.1
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = +1
Query: 226 KKDVALAKVPNAEDKLKVEKLIDACLANKGNSPHQTAW 339
KK L + P A+D ++EK+++AC N T W
Sbjct: 95 KKKHVLVEKPPAQDATELEKIVEACEYNGVQFMDGTIW 132
>At5g64710.2 68418.m08135 expressed protein contains Pfam profile
PF04396: Protein of unknown function, DUF537
Length = 716
Score = 27.9 bits (59), Expect = 5.4
Identities = 19/73 (26%), Positives = 32/73 (43%), Gaps = 3/73 (4%)
Frame = +1
Query: 22 VVLAQALTDEQKENLKKHRADCLAETKADEQLVNKLKTGDFKTENEPLKKYALCMLI--- 192
VV + + K+N K + + ++ ++ V +K D K + EP+K L +
Sbjct: 237 VVSVEKMCQNMKQNDKDVKEESHQLQESSQEFVQVMKLMDVKAKEEPVKANQLAITAVDD 296
Query: 193 KSQLMTKDGKFKK 231
S KDG KK
Sbjct: 297 VSSFEEKDGFLKK 309
>At5g64710.1 68418.m08134 expressed protein contains Pfam profile
PF04396: Protein of unknown function, DUF537
Length = 841
Score = 27.9 bits (59), Expect = 5.4
Identities = 19/73 (26%), Positives = 32/73 (43%), Gaps = 3/73 (4%)
Frame = +1
Query: 22 VVLAQALTDEQKENLKKHRADCLAETKADEQLVNKLKTGDFKTENEPLKKYALCMLI--- 192
VV + + K+N K + + ++ ++ V +K D K + EP+K L +
Sbjct: 362 VVSVEKMCQNMKQNDKDVKEESHQLQESSQEFVQVMKLMDVKAKEEPVKANQLAITAVDD 421
Query: 193 KSQLMTKDGKFKK 231
S KDG KK
Sbjct: 422 VSSFEEKDGFLKK 434
>At3g04810.2 68416.m00521 protein kinase, putative similar to
LSTK-1-like kinase [Lycopersicon esculentum]
GI:15637110; contains Pfam profile: PF00069 Eukaryotic
protein kinase domain
Length = 578
Score = 27.9 bits (59), Expect = 5.4
Identities = 23/85 (27%), Positives = 36/85 (42%), Gaps = 1/85 (1%)
Frame = +1
Query: 16 VCVVLAQALTDEQKENLKKHRADCLAETKADEQLVNKLKTGDFKTENEPLKKYALCMLIK 195
VC+++ + E +KK E K + LV L ++ N L + C I
Sbjct: 77 VCIIIGYCKGGDMAEAIKKTNGVHFTEEKLCKWLVQILLALEYLHANHILHRDVKCSNI- 135
Query: 196 SQLMTKDGKFK-KDVALAKVPNAED 267
+TKD + D LAKV ++D
Sbjct: 136 --FLTKDQDIRLGDFGLAKVLTSDD 158
>At3g04810.1 68416.m00520 protein kinase, putative similar to
LSTK-1-like kinase [Lycopersicon esculentum]
GI:15637110; contains Pfam profile: PF00069 Eukaryotic
protein kinase domain
Length = 606
Score = 27.9 bits (59), Expect = 5.4
Identities = 23/85 (27%), Positives = 36/85 (42%), Gaps = 1/85 (1%)
Frame = +1
Query: 16 VCVVLAQALTDEQKENLKKHRADCLAETKADEQLVNKLKTGDFKTENEPLKKYALCMLIK 195
VC+++ + E +KK E K + LV L ++ N L + C I
Sbjct: 77 VCIIIGYCKGGDMAEAIKKTNGVHFTEEKLCKWLVQILLALEYLHANHILHRDVKCSNI- 135
Query: 196 SQLMTKDGKFK-KDVALAKVPNAED 267
+TKD + D LAKV ++D
Sbjct: 136 --FLTKDQDIRLGDFGLAKVLTSDD 158
>At1g33670.1 68414.m04165 leucine-rich repeat family protein
contains leucine rich-repeat domains Pfam:PF00560,
INTERPRO:IPR001611; similar to receptor kinase-like
protein GB:AAB82755 GI:2586083 from [Oryza
longistaminata] (Science 270 (5243), 1804-1806 (1995))
Length = 455
Score = 27.9 bits (59), Expect = 5.4
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = -1
Query: 258 IRHFSQSDVLLEFSVLGHQLRFD 190
IR F+Q D ++EF G++L+FD
Sbjct: 362 IRFFNQMDFMVEFHAPGNKLQFD 384
>At5g60620.1 68418.m07608 phospholipid/glycerol acyltransferase
family protein contains Pfam PF01553: Acyltransferase
Length = 376
Score = 27.5 bits (58), Expect = 7.2
Identities = 8/15 (53%), Positives = 13/15 (86%)
Frame = +3
Query: 519 NFNLYLFGKFCFGII 563
N+N+YLF +CFG++
Sbjct: 76 NWNIYLFPLYCFGVV 90
>At3g20860.1 68416.m02637 protein kinase family protein contains
protein kinase domain, Pfam:PF00069; contains
serine/threonine protein kinase domain,
INTERPRO:IPR002290
Length = 427
Score = 27.5 bits (58), Expect = 7.2
Identities = 22/85 (25%), Positives = 37/85 (43%), Gaps = 1/85 (1%)
Frame = +1
Query: 16 VCVVLAQALTDEQKENLKKHRADCLAETKADEQLVNKLKTGDFKTENEPLKKYALCMLIK 195
VC+V + + + +KK R +E K +V L D+ N L + C I
Sbjct: 87 VCIVTSYCEGGDMTQMIKKSRGVFASEEKLCRWMVQLLLAIDYLHNNRVLHRDLKCSNI- 145
Query: 196 SQLMTKDGKFK-KDVALAKVPNAED 267
+TK+ + + D LAK+ +D
Sbjct: 146 --FLTKENEVRLGDFGLAKLLGKDD 168
>At2g04230.1 68415.m00410 F-box family protein contains F-box domain
Pfam:PF00646
Length = 448
Score = 27.5 bits (58), Expect = 7.2
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = +1
Query: 256 NAEDKLKVEKLIDACLANKGNSPHQTAWNYVKCYHE 363
++ KL++ KL D L + +P + WN KC E
Sbjct: 333 DSSPKLQILKLTDVYLHDNKTNPDERKWNPPKCAPE 368
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,710,339
Number of Sequences: 28952
Number of extensions: 258124
Number of successful extensions: 724
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 713
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 724
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1187288784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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