Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0634
         (544 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC902.05c |idh2|glu2|isocitrate dehydrogenase |Schizosaccharom...   187   1e-48
SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase |Schizosaccharom...   153   1e-38
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ...    76   3e-15
SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase Le...    37   0.002
SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces pomb...    27   1.8  
SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||...    26   3.1  
SPAC6B12.10c |spp1|pri1|DNA primase catalytic subunit Spp1 |Schi...    26   4.1  
SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1||...    26   4.1  
SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr 1|...    25   5.5  
SPBC11G11.07 ||SPBC18H10.01|karyopherin|Schizosaccharomyces pomb...    25   7.2  
SPBC215.09c |erg10||acetyl-CoA C-acetyltransferase Erg10 |Schizo...    25   9.5  
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces...    25   9.5  

>SPBC902.05c |idh2|glu2|isocitrate dehydrogenase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 378

 Score =  187 bits (455), Expect = 1e-48
 Identities = 95/162 (58%), Positives = 116/162 (71%), Gaps = 5/162 (3%)
 Frame = +1

Query: 73  ATRAGAAQY-----STGVRKVTLIPGHGIGPEITVAVQKIFEAAKVPIEWEEVDVTAVRG 237
           +T+A A  Y     + G   VT+I G GIGPEI  +V++IF+AAKVPIEWE V V  +  
Sbjct: 28  STKAAAGTYEGVKNANGNYTVTMIAGDGIGPEIAQSVERIFKAAKVPIEWERVKVYPIL- 86

Query: 238 PDGKFGIPQKAIDSVNANKIGLKGPLMTPVGKGYRSLNLALRKEFDLYANVRPCKSLEGI 417
            +G   IP  A +SV  NK+ LKGPL TP+GKG+ S+NL LR+ F L+ANVRPC S+ G 
Sbjct: 87  KNGTTTIPDDAKESVRKNKVALKGPLATPIGKGHVSMNLTLRRTFGLFANVRPCVSITGY 146

Query: 418 KTLYDNVDVVTIRENTEGEYSGIEHEIVDGVVQSIKLITEEA 543
           KT YDNV+ V IRENTEGEYSGIEHE++ GVVQSIKLIT  A
Sbjct: 147 KTPYDNVNTVLIRENTEGEYSGIEHEVIPGVVQSIKLITRAA 188


>SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 356

 Score =  153 bits (372), Expect = 1e-38
 Identities = 80/146 (54%), Positives = 103/146 (70%), Gaps = 2/146 (1%)
 Frame = +1

Query: 106 GVRKVTLIPGHGIGPEITVAVQKIFEAAKVPIEWEEVDVTAVRGPDGKFGIP-QKAIDSV 282
           G   VTLIPG GIG E + AV +IF+ A VPIE+EE+DVT +   +   G    +AI S+
Sbjct: 19  GKYTVTLIPGDGIGRETSNAVTEIFKTANVPIEFEEIDVTGMEKNNKSSGDALHEAIQSL 78

Query: 283 NANKIGLKGPLMTPVGKG-YRSLNLALRKEFDLYANVRPCKSLEGIKTLYDNVDVVTIRE 459
             NK+GLKG L TP  KG + S N+ALRKE D+YA++   K++ G KT +DNVD   IRE
Sbjct: 79  KRNKVGLKGILFTPFEKGGHTSFNVALRKELDIYASLVLIKNIPGFKTRHDNVDFAIIRE 138

Query: 460 NTEGEYSGIEHEIVDGVVQSIKLITE 537
           NTEGEYSG+EH+ V GVV+S+K+ITE
Sbjct: 139 NTEGEYSGLEHQSVPGVVESLKIITE 164


>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 362

 Score = 76.2 bits (179), Expect = 3e-15
 Identities = 55/151 (36%), Positives = 85/151 (56%), Gaps = 11/151 (7%)
 Frame = +1

Query: 124 LIPGHGIGPEITVAVQKIFE--AAKVPIEWEEVDVTAVRGPDGKFG--IPQKAIDSVNAN 291
           LIP  GIG E+  A +++ E   AK  ++++ +D+ A  G   + G  +P++ ++ +   
Sbjct: 11  LIPADGIGKEVVPAARRLMENLPAKHKLKFDFIDLDAGWGTFERTGKALPERTVERLKTE 70

Query: 292 -KIGLKGPLMTPVGK--GYRSLNLALRKEFDLYANVRPCKSLEGIKTLYDNVDVVTIREN 462
               L G + +P  K  GY S  +ALRK+  LYANVRP KSL+G K     VD+V +REN
Sbjct: 71  CNAALFGAVQSPTHKVAGYSSPIVALRKKMGLYANVRPVKSLDGAKG--KPVDLVIVREN 128

Query: 463 TEGEYSGIEHEIVD----GVVQSIKLITEEA 543
           TE  Y   E  + +     V ++I+ I+EEA
Sbjct: 129 TECLYVKEERMVQNTPGKRVAEAIRRISEEA 159


>SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase
           Leu1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 371

 Score = 37.1 bits (82), Expect = 0.002
 Identities = 43/142 (30%), Positives = 64/142 (45%), Gaps = 18/142 (12%)
 Frame = +1

Query: 112 RKVTLIPGHGIGPEITVA---VQKIFEAAKVPIEWEEVDVTAVRGPDGKFGIP-----QK 267
           +K+ ++PG  IGPEI  +   V K+ E  +  ++ E  +          +G P      K
Sbjct: 4   KKIVVLPGDHIGPEIVASALEVLKVVEKKRPELKLEFEEHKIGGASIDAYGTPLTDETVK 63

Query: 268 AIDSVNANKIG-LKGPLMT-PVGKGYRSLNLALRKEFDLYANVRPC----KSLEGIKTL- 426
           A    +   +G + GP  T P  +  + L L LRK   ++AN+RPC    KSL     L 
Sbjct: 64  ACLEADGVLLGAVGGPEWTNPNCRPEQGL-LKLRKSMGVWANLRPCNFASKSLVKYSPLK 122

Query: 427 ---YDNVDVVTIRENTEGEYSG 483
               + VD   +RE T G Y G
Sbjct: 123 PEIVEGVDFCVVRELTGGCYFG 144


>SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 713

 Score = 27.1 bits (57), Expect = 1.8
 Identities = 17/57 (29%), Positives = 30/57 (52%)
 Frame = -3

Query: 485 IPEYSPSVFSLIVTTSTLSYSVLIPSKLLQGLTLAYKSNSFLRAKLSDL*PLPTGVI 315
           IPEYS    + +  T+   +++  P+K + G  +   +N +L  K++    LPTG I
Sbjct: 516 IPEYSKLNVTAVNLTNHSYWNLASPNKTIDGTIIKSTTNVYL--KVNSETSLPTGDI 570


>SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 3971

 Score = 26.2 bits (55), Expect = 3.1
 Identities = 33/133 (24%), Positives = 59/133 (44%), Gaps = 7/133 (5%)
 Frame = -3

Query: 527  SLMDCTTPSTISCSIPEYSPSVFSLIVTTST--LSYSVLIPSKLLQGLTLAYKSNSFLRA 354
            S+++ +TP T S  +   +P   S +V TST   S SVL  S  +   T+   S     +
Sbjct: 760  SILNSSTPITSSSVLNSSTPITSSTVVNTSTPITSSSVLNSSTPITSSTVLNSSTPITSS 819

Query: 353  K-LSDL*PLPTGVIRGPFKPILLAFTESIAFWGIPNLPSGPLTAVTS-TSSHSIGT---L 189
              L+   P+ +  +     PI    + ++     P   S  L + T  TSS ++ T   +
Sbjct: 820  SVLNSSTPITSSTVVNTSTPIT---SSTVVNSSTPITSSSVLNSSTPITSSTALNTSTPI 876

Query: 188  AASKIF*TATVIS 150
             +S +  ++T I+
Sbjct: 877  TSSSVLNSSTPIT 889



 Score = 26.2 bits (55), Expect = 3.1
 Identities = 34/137 (24%), Positives = 57/137 (41%), Gaps = 3/137 (2%)
 Frame = -3

Query: 521  MDCTTPSTISCSIPEYSPSVFSLIVTTST--LSYSVLIPSKLLQGLTLAYKSNSFLRAK- 351
            ++ +TP T S  +   +P   S +V TST   S +V+  S  +   T+   S     +  
Sbjct: 2778 LNTSTPITSSSVLNSSTPITSSTVVNTSTPITSSTVVNSSTPITSSTVLNSSTPITSSSV 2837

Query: 350  LSDL*PLPTGVIRGPFKPILLAFTESIAFWGIPNLPSGPLTAVTSTSSHSIGTLAASKIF 171
            L+   P+ +        PI    + S+     P   S  L   TS +S S+  L +S   
Sbjct: 2838 LNSSTPITSSTALNTSTPIT---SSSVLNSSTPITSSTALNTSTSITSSSV--LNSSTPI 2892

Query: 170  *TATVISGPIPCPGMSV 120
             ++TV++   P    SV
Sbjct: 2893 TSSTVVNTSTPITSSSV 2909



 Score = 25.8 bits (54), Expect = 4.1
 Identities = 34/132 (25%), Positives = 56/132 (42%), Gaps = 5/132 (3%)
 Frame = -3

Query: 527  SLMDCTTPSTISCSIPEYSPSVFSLIVTTST--LSYSVLIPSKLLQGLTLAYKSNSFLRA 354
            S+++ +TP T S  +   +P   S  + TST   S SVL  S  +   T+   S     +
Sbjct: 3364 SVLNSSTPITSSTVVNSSTPITSSTALNTSTPITSSSVLNSSTPITSSTVVNSSTPITSS 3423

Query: 353  -KLSDL*PLPTGVIRGPFKPILLA--FTESIAFWGIPNLPSGPLTAVTSTSSHSIGTLAA 183
              L+   P+ +  +     PI  +     S A      L S   T +TS+S  +  T  +
Sbjct: 3424 TALNTSTPITSSTVVNSSTPITSSSVLNSSTAIASSSILNSS--TPITSSSVLNSSTPIS 3481

Query: 182  SKIF*TATVISG 147
            S    T++V+ G
Sbjct: 3482 SSTVITSSVVIG 3493



 Score = 25.4 bits (53), Expect = 5.5
 Identities = 33/133 (24%), Positives = 58/133 (43%), Gaps = 7/133 (5%)
 Frame = -3

Query: 527 SLMDCTTPSTISCSIPEYSPSVFSLIVTTST--LSYSVLIPSKLLQGLTLAYKSNSFLRA 354
           ++++ +TP T S  +   +P   S +V TST    YSVL  S  +   T+   S     +
Sbjct: 556 TVVNTSTPITSSSVLNSSTPITSSTVVNTSTPITRYSVLNSSTPITSSTVLNSSTPITSS 615

Query: 353 K-LSDL*PLPTGVIRGPFKPILLAFTESIAFWGIPNLPSGPLTAVTS-TSSHSIGT---L 189
             L+   P+ +        PI    + S+     P   S  L + T  TSS ++ T   +
Sbjct: 616 SVLNSSTPITSSTALNTSTPIT---SSSVLNSSTPITSSSVLNSSTPITSSTALNTSTPI 672

Query: 188 AASKIF*TATVIS 150
            +S +  ++T I+
Sbjct: 673 TSSSVLNSSTPIT 685



 Score = 24.6 bits (51), Expect = 9.5
 Identities = 35/147 (23%), Positives = 67/147 (45%), Gaps = 11/147 (7%)
 Frame = -3

Query: 527  SLMDCTTPSTISCSIPEYSPSVFSLIVTTST-LSYSVLIPSKLLQGLTLAYKSNSFLRAK 351
            ++++ +TP T S  +   +P   S +V TST ++ S ++ S      + A  +++ + + 
Sbjct: 2260 TVVNTSTPITSSTVVNSSTPITSSTVVNTSTPITSSTVVNSSTPITSSTALNTSTPITSS 2319

Query: 350  --LSDL*PLPTGVIRGPFKPI----LLAFTESIAFWGIPNLPSGPLTAV----TSTSSHS 201
              L+   P+ +  +     PI    +L  +  I    + N  S P+T+     TSTS  S
Sbjct: 2320 SVLNSSTPITSSTVVNTSTPITSSSVLNSSTPITSSTVVN-TSTPITSSTALNTSTSITS 2378

Query: 200  IGTLAASKIF*TATVISGPIPCPGMSV 120
               L +S    ++TV++   P    SV
Sbjct: 2379 SSVLNSSTPITSSTVVNTSTPITSSSV 2405


>SPAC6B12.10c |spp1|pri1|DNA primase catalytic subunit Spp1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 454

 Score = 25.8 bits (54), Expect = 4.1
 Identities = 11/29 (37%), Positives = 15/29 (51%)
 Frame = -2

Query: 252 EFAIWTPNSCYIHFLPFDRYFSCFEDFLN 166
           EFA   PN  YI +L F  +    ++ LN
Sbjct: 66  EFAFTLPNDAYIRYLSFSNWEELKKEALN 94


>SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1116

 Score = 25.8 bits (54), Expect = 4.1
 Identities = 9/32 (28%), Positives = 21/32 (65%)
 Frame = -2

Query: 525 LDGLHDPVDDLVLDTGILTLGVLSDRDHVNVV 430
           ++G HD +   ++D+    + +++D DH+N+V
Sbjct: 862 INGRHDQLRKDIVDSINAEISMIADLDHLNIV 893


>SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 927

 Score = 25.4 bits (53), Expect = 5.5
 Identities = 11/26 (42%), Positives = 14/26 (53%)
 Frame = -2

Query: 156 DLGPNTMSRDERNLAHASAVLSSAGS 79
           DL PN   +++RNL    A L   GS
Sbjct: 2   DLKPNIRRKEKRNLLKGEAALEKKGS 27


>SPBC11G11.07 ||SPBC18H10.01|karyopherin|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 955

 Score = 25.0 bits (52), Expect = 7.2
 Identities = 16/46 (34%), Positives = 26/46 (56%), Gaps = 2/46 (4%)
 Frame = +1

Query: 322 PVGKGYRSLN--LALRKEFDLYANVRPCKSLEGIKTLYDNVDVVTI 453
           PV   Y+S++  LA   +  L A V+   +   ++ L DN+D+VTI
Sbjct: 584 PVANVYQSVHSFLAPSLQSILLAQVKLNPTQAELEALADNIDIVTI 629


>SPBC215.09c |erg10||acetyl-CoA C-acetyltransferase Erg10
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 395

 Score = 24.6 bits (51), Expect = 9.5
 Identities = 12/32 (37%), Positives = 17/32 (53%)
 Frame = +1

Query: 220 VTAVRGPDGKFGIPQKAIDSVNANKIGLKGPL 315
           V+AVR P G FG    ++ +     I +KG L
Sbjct: 9   VSAVRTPMGSFGGSFASLPATKLGSIAIKGAL 40


>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1485

 Score = 24.6 bits (51), Expect = 9.5
 Identities = 10/27 (37%), Positives = 17/27 (62%)
 Frame = -2

Query: 210 LPFDRYFSCFEDFLNGDRDLGPNTMSR 130
           +  D  FS +ED  +GD ++ PNT ++
Sbjct: 1   MSIDADFSDYEDEASGDENVLPNTTTK 27


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.316    0.136    0.382 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,137,358
Number of Sequences: 5004
Number of extensions: 42628
Number of successful extensions: 193
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 223909422
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)