Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0626
         (720 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC17G6.16c |ysh1||mRNA cleavage and polyadenylation specificit...   181   1e-46
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||...    29   0.51 
SPBC1709.15c |cft2||cleavage factor two Cft2/polyadenylation fac...    29   0.51 
SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr...    28   1.2  
SPAC1002.19 |urg1||GTP cyclohydrolase II |Schizosaccharomyces po...    26   4.7  
SPAC22A12.01c |pso2|snm1, SPAC56F8.17c, snm1|DNA 5' exonuclease ...    26   4.7  
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po...    25   8.2  

>SPAC17G6.16c |ysh1||mRNA cleavage and polyadenylation specificity
           factor complex subunit Ysh1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 775

 Score =  181 bits (440), Expect = 1e-46
 Identities = 78/145 (53%), Positives = 104/145 (71%)
 Frame = +1

Query: 232 DQLTIRPLGAGQEVGRSCIMLEFKGKKIMLDCGIHPGLSGMDALPFIDLIEADEVDLLLV 411
           D L    LGAG EVGRSC ++++KGK +MLD G+HP  +G+ ALPF D  +   VD+LL+
Sbjct: 35  DLLEFINLGAGNEVGRSCHVIQYKGKTVMLDAGVHPAYTGLSALPFFDEFDLSTVDVLLI 94

Query: 412 SHFHLDHSGALPWFLTKTSFKGRVFMTHATKAIYRWLVSDYIKVSNISTEQMLYTESDLE 591
           SHFHLDH  +LP+ + KT+F+GRVFMTH TKA+ +WL+SDY+KVSN+  E  LY E DL 
Sbjct: 95  SHFHLDHVASLPYVMQKTNFRGRVFMTHPTKAVCKWLLSDYVKVSNVGMEDQLYDEKDLL 154

Query: 592 NSMDRIETINFHEERDVPGCKFWAY 666
            + DRIE +++H   +V G KF  Y
Sbjct: 155 AAFDRIEAVDYHSTIEVEGIKFTPY 179



 Score = 26.6 bits (56), Expect = 3.6
 Identities = 10/19 (52%), Positives = 14/19 (73%)
 Frame = +2

Query: 647 GVNFGLTNAGHVLGAAMFY 703
           G+ F   +AGHVLGA M++
Sbjct: 173 GIKFTPYHAGHVLGACMYF 191


>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 3227

 Score = 29.5 bits (63), Expect = 0.51
 Identities = 20/68 (29%), Positives = 34/68 (50%), Gaps = 3/68 (4%)
 Frame = +1

Query: 460  KTSFKGRVFMTHATKAIYRWLVSDYIKVSNISTEQMLYTES---DLENSMDRIETINFHE 630
            K  + GR+   H ++A+Y+ ++   + V +I +    Y +S    L N +  I T  F  
Sbjct: 2970 KALYDGRLLDCHFSRAVYKHMLHRSVSVKDIESLDPDYYKSLVWMLNNDITDIITEEFAV 3029

Query: 631  ERDVPGCK 654
            E+DV G K
Sbjct: 3030 EKDVFGEK 3037


>SPBC1709.15c |cft2||cleavage factor two Cft2/polyadenylation factor
           CPSF-73 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 797

 Score = 29.5 bits (63), Expect = 0.51
 Identities = 22/82 (26%), Positives = 41/82 (50%), Gaps = 1/82 (1%)
 Frame = +1

Query: 388 DEVDLLLVSHFHLDHSGALPWFLTKTSFKGR-VFMTHATKAIYRWLVSDYIKVSNISTEQ 564
           ++ DL+L+SH  L H G L +   K  +K   ++ T  T  + R  + D IK + IS   
Sbjct: 42  EQPDLILLSHSDLAHIGGLVYAYYKYDWKNAYIYATLPTINMGRMTMLDAIKSNYISD-- 99

Query: 565 MLYTESDLENSMDRIETINFHE 630
              +++D++   D I  + + +
Sbjct: 100 --MSKADVDAVFDSIIPLRYQQ 119


>SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 703

 Score = 28.3 bits (60), Expect = 1.2
 Identities = 14/40 (35%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
 Frame = -1

Query: 249 SYCQLIAFLRRHWISAFSWICSHFVYLQIFIY-RDLTNSL 133
           SY  L A L + WIS +S +C  ++ +Q F++ +DL   +
Sbjct: 4   SYLSLAARLSQCWISPWS-LCCLYILMQFFLFTKDLNTKI 42


>SPAC1002.19 |urg1||GTP cyclohydrolase II |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 439

 Score = 26.2 bits (55), Expect = 4.7
 Identities = 14/42 (33%), Positives = 20/42 (47%)
 Frame = -2

Query: 404 RRSTSSASIRSMKGRASIPDKPGWMPQSNIIFFPLNSNMMHD 279
           +R+   A +R M+ +A +PD   W     I      SNM HD
Sbjct: 343 KRTEELAGVRDMRFQALMPDILHWFGIKKIDRMLSMSNMKHD 384


>SPAC22A12.01c |pso2|snm1, SPAC56F8.17c, snm1|DNA 5' exonuclease
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 560

 Score = 26.2 bits (55), Expect = 4.7
 Identities = 10/19 (52%), Positives = 12/19 (63%)
 Frame = +1

Query: 388 DEVDLLLVSHFHLDHSGAL 444
           D V+   +SHFH DH G L
Sbjct: 206 DGVEAYFLSHFHSDHYGGL 224


>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 492

 Score = 25.4 bits (53), Expect = 8.2
 Identities = 13/43 (30%), Positives = 21/43 (48%)
 Frame = +1

Query: 511 YRWLVSDYIKVSNISTEQMLYTESDLENSMDRIETINFHEERD 639
           Y  L  +Y   SN+   ++  TE+D+  S+    TI F +  D
Sbjct: 395 YEKLAEEYSDDSNVVVAKIDATENDISVSISGFPTIMFFKAND 437


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,980,723
Number of Sequences: 5004
Number of extensions: 62441
Number of successful extensions: 165
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 165
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)