Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0625
(756 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 151 1e-37
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 91 2e-19
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 75 1e-14
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 62 1e-10
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 47 3e-06
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 45 1e-05
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 31 0.13
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 31 0.18
SPAC823.03 |ppk15||serine/threonine protein kinase Ppk15 |Schizo... 29 0.95
SPBC2A9.09 |||phosducin family protein|Schizosaccharomyces pombe... 28 1.3
SPBC2G2.02 |syj1||inositol-polyphosphate 5-phosphatase |Schizosa... 28 1.7
SPAC25A8.03c ||SPAC3C7.15c|DUF185 protein|Schizosaccharomyces po... 27 2.2
SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces ... 27 2.9
SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr ... 27 2.9
SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosacch... 27 3.8
SPAC167.05 ||SPAC57A7.01|Usp |Schizosaccharomyces pombe|chr 1|||... 26 5.0
SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces p... 26 5.0
SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4 |Schizosa... 25 8.8
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 151 bits (365), Expect = 1e-37
Identities = 88/222 (39%), Positives = 121/222 (54%), Gaps = 10/222 (4%)
Frame = +3
Query: 75 IQNDAHFQTEMANAGTKLVVVDFTATWCPPCQRIAPFFEQLPAKF--PRAVFLKVDVDRC 248
I++ H+ + + +G + VD A WC PC+ I+P F QL +K+ P+ VF KV+VD
Sbjct: 6 IRSYQHWISTIPKSG--YLAVDCYADWCGPCKAISPLFSQLASKYASPKFVFAKVNVDEQ 63
Query: 249 ADTASAQGVSAMPTFIFYRNRTKIDRLEGGNTTVLENKV----RQYYGSDVAEEDEDNTV 416
AS GV AMPTF+F+ N +ID L G N L+ KV + G+ V
Sbjct: 64 RQIASGLGVKAMPTFVFFENGKQIDMLTGANPQALKEKVALISSKATGTGALASSSSAPV 123
Query: 417 AGHMDLITFITKSECECLNEADDHPLAQAL-TNDRGYLASYCDEQLIINISFNQLVKLHS 593
G L I + ECLN+ DDH L A +N +L S DEQL+I I F ++VK+HS
Sbjct: 124 KGFASLQGCIENPQLECLNQQDDHDLKSAFNSNPSSFLESDVDEQLMIYIPFLEVVKVHS 183
Query: 594 IKI---KGPADKGPKSIKLFINQPRTLDFDQAAGYSSVQDLE 710
I I KG PK+IKL+INQP L F+ A ++ Q +E
Sbjct: 184 IAITPVKGETSSAPKTIKLYINQPNNLSFEDAESFTPTQVIE 225
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 91.1 bits (216), Expect = 2e-19
Identities = 41/82 (50%), Positives = 54/82 (65%)
Frame = +3
Query: 123 KLVVVDFTATWCPPCQRIAPFFEQLPAKFPRAVFLKVDVDRCADTASAQGVSAMPTFIFY 302
KLVVVDF ATWC PC+ IAP FEQ + A F+KVDVD+ ++ A+ GV AMP+F Y
Sbjct: 19 KLVVVDFFATWCGPCKAIAPKFEQFSNTYSDATFIKVDVDQLSEIAAEAGVHAMPSFFLY 78
Query: 303 RNRTKIDRLEGGNTTVLENKVR 368
+N KI+ + G N LE ++
Sbjct: 79 KNGEKIEEIVGANPAKLEASIK 100
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 74.5 bits (175), Expect = 1e-14
Identities = 31/84 (36%), Positives = 51/84 (60%)
Frame = +3
Query: 123 KLVVVDFTATWCPPCQRIAPFFEQLPAKFPRAVFLKVDVDRCADTASAQGVSAMPTFIFY 302
K+ VVDF A WC PC+ + PF E+L + +A F+ V+ D+ +D A GV A+PT + +
Sbjct: 36 KVTVVDFYADWCGPCKYLKPFLEKLSEQNQKASFIAVNADKFSDIAQKNGVYALPTMVLF 95
Query: 303 RNRTKIDRLEGGNTTVLENKVRQY 374
R ++DR+ G + L + + +Y
Sbjct: 96 RKGQELDRIVGADVKTLSSLLAKY 119
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 61.7 bits (143), Expect = 1e-10
Identities = 26/94 (27%), Positives = 51/94 (54%)
Frame = +3
Query: 93 FQTEMANAGTKLVVVDFTATWCPPCQRIAPFFEQLPAKFPRAVFLKVDVDRCADTASAQG 272
FQ + N ++++++F A W PC+++ F+Q AVFLK++ ++ +D A +
Sbjct: 11 FQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAVFLKIEAEKFSDIAESFD 70
Query: 273 VSAMPTFIFYRNRTKIDRLEGGNTTVLENKVRQY 374
V+A+P F+ + R+ G N L+ + +Y
Sbjct: 71 VNAVPLFVLIHGAKVLARISGANPQKLKAAIDEY 104
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 46.8 bits (106), Expect = 3e-06
Identities = 22/66 (33%), Positives = 36/66 (54%), Gaps = 3/66 (4%)
Frame = +3
Query: 114 AGTKLVVVDFTATWCPPCQRIAPFFEQLPAKFP---RAVFLKVDVDRCADTASAQGVSAM 284
A K +++F ATWC C+ +AP +E+L A F + K+D D +D A ++
Sbjct: 37 ASKKGALIEFYATWCGHCKSLAPVYEELGALFEDHNDVLIGKIDADTHSDVADKYHITGF 96
Query: 285 PTFIFY 302
PT I++
Sbjct: 97 PTLIWF 102
Score = 45.6 bits (103), Expect = 8e-06
Identities = 30/103 (29%), Positives = 49/103 (47%), Gaps = 5/103 (4%)
Frame = +3
Query: 123 KLVVVDFTATWCPPCQRIAPFFEQLPAKF---PRAVFLKVDVDRCADTASAQGVSAMPTF 293
K V+V+F A WC C+R+AP +E L F P +K++ D AD V++ PT
Sbjct: 159 KDVLVEFYADWCGYCKRLAPTYETLGKVFKNEPNVEIVKINADVFADIGRLHEVASFPTI 218
Query: 294 IFY--RNRTKIDRLEGGNTTVLENKVRQYYGSDVAEEDEDNTV 416
F+ ++ K + EG + LE+ + + D T+
Sbjct: 219 KFFPKDDKDKPELYEGDRS--LESLIEYINKKSGTQRSPDGTL 259
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 44.8 bits (101), Expect = 1e-05
Identities = 25/80 (31%), Positives = 42/80 (52%), Gaps = 5/80 (6%)
Frame = +3
Query: 120 TKLVVVDFTATWCPPCQRIAPFFEQLPAKF---PRAVFLKVDVDRCADTASAQGVSAMPT 290
TK V+V+F A WC C+ +AP +E+L ++ V K+D + + +S PT
Sbjct: 373 TKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAKID---ATENDISVSISGFPT 429
Query: 291 FIFYRNRTKID--RLEGGNT 344
+F++ K++ R EG T
Sbjct: 430 IMFFKANDKVNPVRYEGDRT 449
Score = 37.9 bits (84), Expect = 0.002
Identities = 19/73 (26%), Positives = 34/73 (46%), Gaps = 2/73 (2%)
Frame = +3
Query: 123 KLVVVDFTATWCPPCQRIAPFFEQLPAKFPR--AVFLKVDVDRCADTASAQGVSAMPTFI 296
K+++V F A WC C+ +AP +E + + ++VD D S + PT
Sbjct: 40 KVLMVKFYAPWCGHCKALAPEYESAADELEKDGISLVEVDCTEEGDLCSEYSIRGYPTLN 99
Query: 297 FYRNRTKIDRLEG 335
++N +I + G
Sbjct: 100 VFKNGKQISQYSG 112
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 31.5 bits (68), Expect = 0.13
Identities = 21/80 (26%), Positives = 34/80 (42%), Gaps = 3/80 (3%)
Frame = +3
Query: 60 GLATVIQNDAHFQTEMANAGTKLVVVDFTATWCPPCQRIAPFFEQLPAKFPR---AVFLK 230
G T+ N +F+ + G LVV F A WC C+++ P +++L + +
Sbjct: 30 GSNTIELNSKNFRKFVKAKGPSLVV--FYAPWCGYCKKLVPTYQKLASNLHSLLPVTAVD 87
Query: 231 VDVDRCADTASAQGVSAMPT 290
D D+ S V PT
Sbjct: 88 CDADQNRAVCSQYQVQGFPT 107
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 31.1 bits (67), Expect = 0.18
Identities = 17/68 (25%), Positives = 32/68 (47%), Gaps = 4/68 (5%)
Frame = +3
Query: 156 CPPCQRIAPFFEQLPAKFPRAV----FLKVDVDRCADTASAQGVSAMPTFIFYRNRTKID 323
C C+R+ P ++ + K V F +VD + +S + A+PT Y+N ++
Sbjct: 54 CGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSKELSSCANIRAVPTLYLYQNGEIVE 113
Query: 324 RLEGGNTT 347
+ G +T
Sbjct: 114 EVPFGAST 121
>SPAC823.03 |ppk15||serine/threonine protein kinase Ppk15
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 534
Score = 28.7 bits (61), Expect = 0.95
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = -2
Query: 176 NPLARWTPSCCKVHND*LGSSVSHFSLE 93
NPL RWTP K H G+S S + ++
Sbjct: 442 NPLKRWTPDQAKNHPFITGASFSQYCID 469
>SPBC2A9.09 |||phosducin family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 233
Score = 28.3 bits (60), Expect = 1.3
Identities = 17/71 (23%), Positives = 33/71 (46%), Gaps = 1/71 (1%)
Frame = +3
Query: 93 FQTEMANAGTKL-VVVDFTATWCPPCQRIAPFFEQLPAKFPRAVFLKVDVDRCADTASAQ 269
+ E+ +A ++ VVV P C+ +A E+L +P+ F+K+ + +
Sbjct: 97 YTAEVTDASKEVFVVVHMFQDSLPACKLLAAILERLAPMYPQIKFVKIPGKQAVENYPE- 155
Query: 270 GVSAMPTFIFY 302
+ MPT + Y
Sbjct: 156 --AMMPTLLIY 164
>SPBC2G2.02 |syj1||inositol-polyphosphate 5-phosphatase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1076
Score = 27.9 bits (59), Expect = 1.7
Identities = 15/45 (33%), Positives = 20/45 (44%)
Frame = +3
Query: 171 RIAPFFEQLPAKFPRAVFLKVDVDRCADTASAQGVSAMPTFIFYR 305
++ PFF +LP FP + D DT+ V A I YR
Sbjct: 778 KVFPFFSELPITFPPTYKFDIGTD-IYDTSDKHRVPAWTDRILYR 821
>SPAC25A8.03c ||SPAC3C7.15c|DUF185 protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 467
Score = 27.5 bits (58), Expect = 2.2
Identities = 18/64 (28%), Positives = 30/64 (46%)
Frame = +3
Query: 183 FFEQLPAKFPRAVFLKVDVDRCADTASAQGVSAMPTFIFYRNRTKIDRLEGGNTTVLENK 362
FFE+L +FP L +DV C S G++A P+ + N ++ V +N+
Sbjct: 335 FFERLSKQFPHHSLLLMDV--CHVDKSLPGINA-PSVLSMENDFSTKKMSSNIGHVFQNE 391
Query: 363 VRQY 374
+Y
Sbjct: 392 TVKY 395
>SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 561
Score = 27.1 bits (57), Expect = 2.9
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = -2
Query: 326 SVDFGPVSVENECGHCTDALSTRCIGAS 243
SV+ PVSV+NE H S+R AS
Sbjct: 138 SVELNPVSVDNEVAHGLAVFSSRDAAAS 165
>SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1337
Score = 27.1 bits (57), Expect = 2.9
Identities = 13/26 (50%), Positives = 15/26 (57%)
Frame = +3
Query: 414 VAGHMDLITFITKSECECLNEADDHP 491
V H+D I FITKS C + E D P
Sbjct: 1285 VVNHIDRIMFITKSLCGEIFEIDKWP 1310
>SPBC13G1.10c |mug81||ATP-dependent RNA helicase
Slh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1935
Score = 26.6 bits (56), Expect = 3.8
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = +1
Query: 151 LGVHRAKGLPLFSSSYQQSFREQYF 225
LGV+R KGL FSS+++ EQ+F
Sbjct: 471 LGVNRYKGLFYFSSAFRPCPIEQHF 495
>SPAC167.05 ||SPAC57A7.01|Usp |Schizosaccharomyces pombe|chr
1|||Manual
Length = 601
Score = 26.2 bits (55), Expect = 5.0
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +3
Query: 339 NTTVLENKVRQYYGSDVAEEDEDNTVAGHM 428
NT+ + + + +YGSD+ E ++D A M
Sbjct: 366 NTSFVSSNINNWYGSDLEELEQDVKTAKSM 395
>SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 521
Score = 26.2 bits (55), Expect = 5.0
Identities = 21/81 (25%), Positives = 39/81 (48%)
Frame = +3
Query: 189 EQLPAKFPRAVFLKVDVDRCADTASAQGVSAMPTFIFYRNRTKIDRLEGGNTTVLENKVR 368
E L PR + D+DR + +A GV+++ + N T +L+ +TT +N+
Sbjct: 381 EFLKTYVPRTLDEVYDIDRDTEIVNAGGVNSL-VYKHLLN-TDFQKLDLNDTT--KNQND 436
Query: 369 QYYGSDVAEEDEDNTVAGHMD 431
Q ++ +E D+D M+
Sbjct: 437 QILPNETSESDDDANSISSME 457
>SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1072
Score = 25.4 bits (53), Expect = 8.8
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = +3
Query: 192 QLPAKFPRAVFLKVDVDRCADTASAQ 269
Q PAKFP +V L+ +D +T Q
Sbjct: 386 QTPAKFPTSVLLRQPIDTYLETMFPQ 411
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,802,927
Number of Sequences: 5004
Number of extensions: 52076
Number of successful extensions: 164
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 361294920
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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