BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0601 (363 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g40290.1 68418.m04887 metal-dependent phosphohydrolase HD dom... 29 1.2 At4g35100.1 68417.m04986 plasma membrane intrinsic protein (SIMI... 28 1.6 At5g04950.1 68418.m00524 nicotianamine synthase, putative simila... 27 3.7 At5g41540.1 68418.m05048 disease resistance protein (TIR-NBS-LRR... 27 4.9 At5g20320.1 68418.m02418 DEAD/DEAH box helicase, putative simila... 26 6.5 At3g20770.1 68416.m02627 ethylene-insensitive 3 (EIN3) identical... 26 6.5 At2g16850.1 68415.m01937 plasma membrane intrinsic protein, puta... 26 8.6 At1g07690.1 68414.m00826 hypothetical protein 26 8.6 >At5g40290.1 68418.m04887 metal-dependent phosphohydrolase HD domain-containing protein similar to SP|Q60710 Interferon-gamma inducible protein MG11 {Mus musculus}; contains Pfam profile PF01966: HD domain Length = 448 Score = 28.7 bits (61), Expect = 1.2 Identities = 22/88 (25%), Positives = 41/88 (46%) Frame = +1 Query: 100 YVAKRDFVDHITTVEPIDGVVLLDEEYVRGRKVFGQVVCTFRYGREEDEVMGLNFYKELF 279 Y +D +DH + P D ++ EE + V GRE + + +NFYK+ Sbjct: 337 YAVPKDKIDHFKPITPED-IICFSEEDI----AVTNVKIDLARGRE-NPLECINFYKDYN 390 Query: 280 LASKQIYPAPEKRSYELTRTQERLMKKL 363 A K + P ++ S+ L T + ++ ++ Sbjct: 391 SAEKFVIP-EDRVSHLLPTTYQEMIVRV 417 >At4g35100.1 68417.m04986 plasma membrane intrinsic protein (SIMIP) nearly identical to plasma membrane intrinsic protein [Arabidopsis thaliana] GI:2306917 Length = 280 Score = 28.3 bits (60), Expect = 1.6 Identities = 16/43 (37%), Positives = 19/43 (44%) Frame = +1 Query: 91 LTLYVAKRDFVDHITTVEPIDGVVLLDEEYVRGRKVFGQVVCT 219 L LYV + H P DGV LL + G +F V CT Sbjct: 49 LFLYVTVATVIGHKKQTGPCDGVGLLGIAWAFGGMIFVLVYCT 91 >At5g04950.1 68418.m00524 nicotianamine synthase, putative similar to nicotianamine synthase [Lycopersicon esculentum][GI:4753801], nicotianamine synthase 2 [Hordeum vulgare][GI:4894912] Length = 320 Score = 27.1 bits (57), Expect = 3.7 Identities = 10/24 (41%), Positives = 16/24 (66%) Frame = -1 Query: 222 KRAHHLTEHLAPADVLLVQQHHAV 151 K HL +H+AP VL+++ HA+ Sbjct: 215 KAIEHLEKHMAPGAVLMLRSAHAL 238 >At5g41540.1 68418.m05048 disease resistance protein (TIR-NBS-LRR class), putative domain signature TIR-NBS-LRR exists, suggestive of a disease resistance protein. Length = 1038 Score = 26.6 bits (56), Expect = 4.9 Identities = 15/53 (28%), Positives = 27/53 (50%), Gaps = 1/53 (1%) Frame = +1 Query: 109 KRDFVDHITTVEPIDGVVLL-DEEYVRGRKVFGQVVCTFRYGREEDEVMGLNF 264 +R F+ H+ I G+V D+E RG+++ ++V R R V+ N+ Sbjct: 23 RRKFLSHLRFHFAIKGIVAFKDQEIERGQRIGPELVQAIRESRVSLVVLSKNY 75 >At5g20320.1 68418.m02418 DEAD/DEAH box helicase, putative similar to CAF protein [Arabidopsis thaliana] GI:6102610; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain, PF03368: Domain of unknown function, PF00636: RNase3 domain, PF00035: Double-stranded RNA binding motif Length = 1676 Score = 26.2 bits (55), Expect = 6.5 Identities = 30/103 (29%), Positives = 45/103 (43%) Frame = -1 Query: 363 EFLHQSLLRASQFIGSLLGGRVDLFGGEEQLLVEVEPHHLILLAPVAKRAHHLTEHLAPA 184 E H + S+ IGS L + E LLV +E H +L A + + A ++ H Sbjct: 1039 ELSHLKIKGLSKDIGSSLSLLPSIMHRMENLLVAIELKH-VLSASIPEIA-EVSGHRV-L 1095 Query: 183 DVLLVQQHHAVDRLHRRDVVHEVPLGDVQRQLAVGRALLEHFE 55 + L ++ H L R +V LGD + AV R L H + Sbjct: 1096 EALTTEKCHERLSLERLEV-----LGDAFLKFAVSRHLFLHHD 1133 >At3g20770.1 68416.m02627 ethylene-insensitive 3 (EIN3) identical to ethylene-insensitive3 GI:2224933 from [Arabidopsis thaliana] Length = 628 Score = 26.2 bits (55), Expect = 6.5 Identities = 13/40 (32%), Positives = 19/40 (47%) Frame = -1 Query: 222 KRAHHLTEHLAPADVLLVQQHHAVDRLHRRDVVHEVPLGD 103 K +H+ E L P V+ V ++H V EVP G+ Sbjct: 334 KESHYEVEELKPEKVMNSSNFGMVAKMHDFPVKEEVPAGN 373 >At2g16850.1 68415.m01937 plasma membrane intrinsic protein, putative very strong similarity to plasma membrane intrinsic protein (SIMIP) [Arabidopsis thaliana] GI:2306917 Length = 278 Score = 25.8 bits (54), Expect = 8.6 Identities = 15/43 (34%), Positives = 18/43 (41%) Frame = +1 Query: 91 LTLYVAKRDFVDHITTVEPIDGVVLLDEEYVRGRKVFGQVVCT 219 L LYV + H P GV LL + G +F V CT Sbjct: 47 LFLYVTVATVIGHKNQTGPCGGVGLLGIAWAFGGMIFVLVYCT 89 >At1g07690.1 68414.m00826 hypothetical protein Length = 164 Score = 25.8 bits (54), Expect = 8.6 Identities = 12/37 (32%), Positives = 23/37 (62%) Frame = +1 Query: 103 VAKRDFVDHITTVEPIDGVVLLDEEYVRGRKVFGQVV 213 V+K+D +I T D +++ E+ V+GR++ +VV Sbjct: 30 VSKQDDESYIMTTSDQDSSMVVLEDVVKGRRLLTRVV 66 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 6,154,051 Number of Sequences: 28952 Number of extensions: 99363 Number of successful extensions: 333 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 328 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 333 length of database: 12,070,560 effective HSP length: 73 effective length of database: 9,957,064 effective search space used: 467982008 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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