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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0601
         (363 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g40290.1 68418.m04887 metal-dependent phosphohydrolase HD dom...    29   1.2  
At4g35100.1 68417.m04986 plasma membrane intrinsic protein (SIMI...    28   1.6  
At5g04950.1 68418.m00524 nicotianamine synthase, putative simila...    27   3.7  
At5g41540.1 68418.m05048 disease resistance protein (TIR-NBS-LRR...    27   4.9  
At5g20320.1 68418.m02418 DEAD/DEAH box helicase, putative simila...    26   6.5  
At3g20770.1 68416.m02627 ethylene-insensitive 3 (EIN3) identical...    26   6.5  
At2g16850.1 68415.m01937 plasma membrane intrinsic protein, puta...    26   8.6  
At1g07690.1 68414.m00826 hypothetical protein                          26   8.6  

>At5g40290.1 68418.m04887 metal-dependent phosphohydrolase HD
           domain-containing protein similar to SP|Q60710
           Interferon-gamma inducible protein MG11 {Mus musculus};
           contains Pfam profile PF01966: HD domain
          Length = 448

 Score = 28.7 bits (61), Expect = 1.2
 Identities = 22/88 (25%), Positives = 41/88 (46%)
 Frame = +1

Query: 100 YVAKRDFVDHITTVEPIDGVVLLDEEYVRGRKVFGQVVCTFRYGREEDEVMGLNFYKELF 279
           Y   +D +DH   + P D ++   EE +        V      GRE + +  +NFYK+  
Sbjct: 337 YAVPKDKIDHFKPITPED-IICFSEEDI----AVTNVKIDLARGRE-NPLECINFYKDYN 390

Query: 280 LASKQIYPAPEKRSYELTRTQERLMKKL 363
            A K + P  ++ S+ L  T + ++ ++
Sbjct: 391 SAEKFVIP-EDRVSHLLPTTYQEMIVRV 417


>At4g35100.1 68417.m04986 plasma membrane intrinsic protein (SIMIP)
           nearly identical to plasma membrane intrinsic protein
           [Arabidopsis thaliana] GI:2306917
          Length = 280

 Score = 28.3 bits (60), Expect = 1.6
 Identities = 16/43 (37%), Positives = 19/43 (44%)
 Frame = +1

Query: 91  LTLYVAKRDFVDHITTVEPIDGVVLLDEEYVRGRKVFGQVVCT 219
           L LYV     + H     P DGV LL   +  G  +F  V CT
Sbjct: 49  LFLYVTVATVIGHKKQTGPCDGVGLLGIAWAFGGMIFVLVYCT 91


>At5g04950.1 68418.m00524 nicotianamine synthase, putative similar
           to nicotianamine synthase [Lycopersicon
           esculentum][GI:4753801], nicotianamine synthase 2
           [Hordeum vulgare][GI:4894912]
          Length = 320

 Score = 27.1 bits (57), Expect = 3.7
 Identities = 10/24 (41%), Positives = 16/24 (66%)
 Frame = -1

Query: 222 KRAHHLTEHLAPADVLLVQQHHAV 151
           K   HL +H+AP  VL+++  HA+
Sbjct: 215 KAIEHLEKHMAPGAVLMLRSAHAL 238


>At5g41540.1 68418.m05048 disease resistance protein (TIR-NBS-LRR
           class), putative domain signature TIR-NBS-LRR exists,
           suggestive of a disease resistance protein.
          Length = 1038

 Score = 26.6 bits (56), Expect = 4.9
 Identities = 15/53 (28%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
 Frame = +1

Query: 109 KRDFVDHITTVEPIDGVVLL-DEEYVRGRKVFGQVVCTFRYGREEDEVMGLNF 264
           +R F+ H+     I G+V   D+E  RG+++  ++V   R  R    V+  N+
Sbjct: 23  RRKFLSHLRFHFAIKGIVAFKDQEIERGQRIGPELVQAIRESRVSLVVLSKNY 75


>At5g20320.1 68418.m02418 DEAD/DEAH box helicase, putative similar to
            CAF protein [Arabidopsis thaliana] GI:6102610; contains
            Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
            Helicase conserved C-terminal domain, PF03368: Domain of
            unknown function, PF00636: RNase3 domain, PF00035:
            Double-stranded RNA binding motif
          Length = 1676

 Score = 26.2 bits (55), Expect = 6.5
 Identities = 30/103 (29%), Positives = 45/103 (43%)
 Frame = -1

Query: 363  EFLHQSLLRASQFIGSLLGGRVDLFGGEEQLLVEVEPHHLILLAPVAKRAHHLTEHLAPA 184
            E  H  +   S+ IGS L     +    E LLV +E  H +L A + + A  ++ H    
Sbjct: 1039 ELSHLKIKGLSKDIGSSLSLLPSIMHRMENLLVAIELKH-VLSASIPEIA-EVSGHRV-L 1095

Query: 183  DVLLVQQHHAVDRLHRRDVVHEVPLGDVQRQLAVGRALLEHFE 55
            + L  ++ H    L R +V     LGD   + AV R L  H +
Sbjct: 1096 EALTTEKCHERLSLERLEV-----LGDAFLKFAVSRHLFLHHD 1133


>At3g20770.1 68416.m02627 ethylene-insensitive 3 (EIN3) identical to
           ethylene-insensitive3 GI:2224933 from [Arabidopsis
           thaliana]
          Length = 628

 Score = 26.2 bits (55), Expect = 6.5
 Identities = 13/40 (32%), Positives = 19/40 (47%)
 Frame = -1

Query: 222 KRAHHLTEHLAPADVLLVQQHHAVDRLHRRDVVHEVPLGD 103
           K +H+  E L P  V+       V ++H   V  EVP G+
Sbjct: 334 KESHYEVEELKPEKVMNSSNFGMVAKMHDFPVKEEVPAGN 373


>At2g16850.1 68415.m01937 plasma membrane intrinsic protein,
           putative very strong similarity to plasma membrane
           intrinsic protein (SIMIP) [Arabidopsis thaliana]
           GI:2306917
          Length = 278

 Score = 25.8 bits (54), Expect = 8.6
 Identities = 15/43 (34%), Positives = 18/43 (41%)
 Frame = +1

Query: 91  LTLYVAKRDFVDHITTVEPIDGVVLLDEEYVRGRKVFGQVVCT 219
           L LYV     + H     P  GV LL   +  G  +F  V CT
Sbjct: 47  LFLYVTVATVIGHKNQTGPCGGVGLLGIAWAFGGMIFVLVYCT 89


>At1g07690.1 68414.m00826 hypothetical protein
          Length = 164

 Score = 25.8 bits (54), Expect = 8.6
 Identities = 12/37 (32%), Positives = 23/37 (62%)
 Frame = +1

Query: 103 VAKRDFVDHITTVEPIDGVVLLDEEYVRGRKVFGQVV 213
           V+K+D   +I T    D  +++ E+ V+GR++  +VV
Sbjct: 30  VSKQDDESYIMTTSDQDSSMVVLEDVVKGRRLLTRVV 66


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,154,051
Number of Sequences: 28952
Number of extensions: 99363
Number of successful extensions: 333
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 328
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 333
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 467982008
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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