Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0588
(790 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g45220.1 68416.m04880 serpin, putative / serine protease inhi... 34 0.12
At5g35750.1 68418.m04281 histidine kinase (AHK2) identical to hi... 33 0.16
At1g27320.1 68414.m03328 histidine kinase (AHK3) identical to hi... 33 0.16
At1g35930.1 68414.m04462 replication protein-related weak simila... 31 1.2
At4g11130.1 68417.m01805 RNA-dependent RNA polymerase, putative ... 30 1.5
At5g20640.1 68418.m02452 hypothetical protein contains Pfam prof... 29 3.5
At3g17560.1 68416.m02242 F-box family protein contains F-box dom... 29 4.6
At3g12930.1 68416.m01611 expressed protein contains Pfam domain ... 29 4.6
At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) M... 28 6.1
At4g21100.1 68417.m03051 UV-damaged DNA-binding protein, putativ... 28 6.1
At1g64400.1 68414.m07299 long-chain-fatty-acid--CoA ligase, puta... 28 8.1
>At3g45220.1 68416.m04880 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 393
Score = 33.9 bits (74), Expect = 0.12
Identities = 28/131 (21%), Positives = 60/131 (45%), Gaps = 2/131 (1%)
Frame = +1
Query: 304 NLIMSPITVWTVLAVIAEGASGNTRRQINHALRL-QAKHTNVTRSEFQKISEWLRVNTNT 480
NL+ SP+++ +L +IA G++ T+ QI + L + + N ++ ++ + +
Sbjct: 30 NLVFSPMSINVLLCLIAAGSNCVTKEQILSFIMLPSSDYLNAVLAKTVSVALNDGMERSD 89
Query: 481 IELAKINAIIVDKQRLPQQDFHDNAKTYYETDMITLNFEDAQNSV-NLLNSAISNFTHGK 657
+ L+ + +DK + F D + Y ++F V N +N+ T+G
Sbjct: 90 LHLSTAYGVWIDKSLSFKPSFKDLLENSYNATCNQVDFATKPAEVINEVNAWAEVHTNGL 149
Query: 658 IPKIVDTGSFQ 690
I +I+ S +
Sbjct: 150 IKEILSDDSIK 160
>At5g35750.1 68418.m04281 histidine kinase (AHK2) identical to
histidine kinase AHK2 [Arabidopsis thaliana]
gi|13537196|dbj|BAB40774
Length = 1176
Score = 33.5 bits (73), Expect = 0.16
Identities = 13/35 (37%), Positives = 22/35 (62%)
Frame = +1
Query: 592 FEDAQNSVNLLNSAISNFTHGKIPKIVDTGSFQDF 696
F + N V+ L+ +S F HGKIP +D +F+++
Sbjct: 280 FNVSLNHVHALSILVSTFHHGKIPSAIDQRTFEEY 314
>At1g27320.1 68414.m03328 histidine kinase (AHK3) identical to
histidine kinase AHK3 [Arabidopsis thaliana]
gi|13537198|dbj|BAB40775
Length = 1036
Score = 33.5 bits (73), Expect = 0.16
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = +1
Query: 577 MITLNFEDAQNSVNLLNSAISNFTHGKIPKIVDTGSFQDF 696
M+ F + N V ++ IS F HGKIP +D +F ++
Sbjct: 136 MLQDQFNVSMNHVQAMSILISTFHHGKIPSAIDQRTFSEY 175
>At1g35930.1 68414.m04462 replication protein-related weak
similarity to Replication Protein A 70 kDa DNA-binding
subunit (RP-A) (RF-A) (Replication factor-A protein 1)
(Single-stranded DNA-binding protein) (DMRPA1)
(GI:2498844)(SP:Q24492) [Drosophila melanogaster]
Length = 261
Score = 30.7 bits (66), Expect = 1.2
Identities = 14/49 (28%), Positives = 24/49 (48%)
Frame = -2
Query: 552 IVMKVLLWQTLLIDNNGIDFGEFYCVRVDSEPF*DFLEFASSDVRMLCL 406
+V V W+ +D + EF C R+ +P + +FAS +ML +
Sbjct: 211 VVCAVRFWRIASVDGENVLKSEFGCSRLHIDPSFPYFDFASGVSKMLMI 259
>At4g11130.1 68417.m01805 RNA-dependent RNA polymerase, putative
similar to RNA-directed RNA polymerase [Lycopersicon
esculentum] gi|4038592|emb|CAA71421
Length = 1133
Score = 30.3 bits (65), Expect = 1.5
Identities = 14/53 (26%), Positives = 25/53 (47%), Gaps = 5/53 (9%)
Frame = +2
Query: 161 FWFWQSCHCLSLKIFLRRLISTM-----GSPRRLEIFRLNYCITPRIWSRVKV 304
FW W+S C +++ +I T+ G ++ F L P+++ RV V
Sbjct: 145 FWVWESGDCYKIEVRFEDIIETLSCCVNGDASEIDAFLLKLKYGPKVFKRVTV 197
>At5g20640.1 68418.m02452 hypothetical protein contains Pfam profile
PF04525: Protein of unknown function (DUF567)
Length = 215
Score = 29.1 bits (62), Expect = 3.5
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +1
Query: 571 TDMITLNFEDAQNSVNLLNSAISNFTHGKIPKIVDT 678
T+ + +D ++S + NS+I HGK PKI T
Sbjct: 104 TERLVFTLKDPKDSCLVQNSSIKILVHGKPPKISST 139
>At3g17560.1 68416.m02242 F-box family protein contains F-box domain
Pfam:PF00646
Length = 413
Score = 28.7 bits (61), Expect = 4.6
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = -2
Query: 579 HISFIIGLCIVMKVLLWQTLLIDNNGIDFGEFYCVRVDSEP 457
H+S + C +KV +W T ID + + +F + VD EP
Sbjct: 307 HLSLLYQSCETLKVEIWMTKEIDTTFVSWKKF--LTVDLEP 345
>At3g12930.1 68416.m01611 expressed protein contains Pfam domain
PF02410: Domain of unknown function DUF143
Length = 238
Score = 28.7 bits (61), Expect = 4.6
Identities = 28/123 (22%), Positives = 53/123 (43%), Gaps = 1/123 (0%)
Frame = +1
Query: 253 FSIELLYHTSNLEQSKGN-LIMSPITVWTVLAVIAEGASGNTRRQINHALRLQAKHTNVT 429
F++EL S+++ L + P+ WT +IA S R QI+ + ++ ++
Sbjct: 123 FAVELAKVASDVKAGDIKVLFVKPLVYWTRFFIIATAFS---RPQID---AIGSRMRDLA 176
Query: 430 RSEFQKISEWLRVNTNTIELAKINAIIVDKQRLPQQDFHDNAKTYYETDMITLNFEDAQN 609
++ K++ V N+ L +++ PQ+ F++ Y I L FED
Sbjct: 177 EKKYGKVANG-DVKPNSWTLLDFGDVVIHLFLPPQRTFYNLEDFYGNAMQIELPFEDQSQ 235
Query: 610 SVN 618
N
Sbjct: 236 PRN 238
>At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5)
Monovalent cation:proton antiporter family 2 (CPA2
family) member, PMID:11500563; related to
glutathione-regulated potassium-efflux system protein
[Escherichia coli] GP|606284|gb|AAA58147
Length = 568
Score = 28.3 bits (60), Expect = 6.1
Identities = 17/60 (28%), Positives = 32/60 (53%), Gaps = 1/60 (1%)
Frame = +1
Query: 139 LIMCLLKFLVLAIVPLSFAQNIPKATNLHNG-LTEKIGNFSIELLYHTSNLEQSKGNLIM 315
+++ ++K + A+V +F N+ + H G L +IG F+ LL SNL +G + +
Sbjct: 453 ILVIVIKTAIAAVVVKAFRYNM--RISFHVGVLLAQIGEFAFVLLSRASNLHVIEGKMYL 510
>At4g21100.1 68417.m03051 UV-damaged DNA-binding protein, putative
similar to UV-damaged DNA binding protein (GI:12082087)
[Oryza sativa] and damage-specific DNA binding protein
1, Homo sapiens, PIR2:I38908; contains Pfam PF03178 :
CPSF A subunit region
Length = 1088
Score = 28.3 bits (60), Expect = 6.1
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = -2
Query: 696 EVLETTGVNYLWYFTMGEVGDG*IQ*INRVLCIFEVKCYHIS 571
+VL TG +L Y E+GDG + + VL +EV C I+
Sbjct: 500 QVLLATGGGHLVYL---EIGDGTLTEVKHVLLEYEVSCLDIN 538
>At1g64400.1 68414.m07299 long-chain-fatty-acid--CoA ligase,
putative / long-chain acyl-CoA synthetase, putative
similar to GI:1617270 (MF7P) from [Brassica napus]
Length = 665
Score = 27.9 bits (59), Expect = 8.1
Identities = 18/58 (31%), Positives = 29/58 (50%)
Frame = +1
Query: 238 EKIGNFSIELLYHTSNLEQSKGNLIMSPITVWTVLAVIAEGASGNTRRQINHALRLQA 411
+K+ NF+ + Y N+E+ + + SPI V + EG GN R ++ A L A
Sbjct: 345 KKLFNFAFK--YKHKNMEKGQPHEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAA 400
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,186,525
Number of Sequences: 28952
Number of extensions: 358718
Number of successful extensions: 939
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 914
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 939
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1775300800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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