BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0584 (707 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g09330.1 68414.m01044 expressed protein contains 3 transmembr... 54 1e-07 At1g16180.1 68414.m01938 TMS membrane family protein / tumour di... 33 0.25 At4g06676.1 68417.m01071 expressed protein ; expression supporte... 31 0.57 At1g07010.1 68414.m00746 calcineurin-like phosphoesterase family... 29 2.3 At1g27040.2 68414.m03296 nitrate transporter, putative contains ... 28 5.3 At1g27040.1 68414.m03297 nitrate transporter, putative contains ... 28 5.3 At2g34720.1 68415.m04264 CCAAT-binding transcription factor (CBF... 28 7.0 At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 28 7.0 At2g20210.1 68415.m02363 leucine-rich repeat family protein cont... 27 9.2 >At1g09330.1 68414.m01044 expressed protein contains 3 transmembrane domains; contains Pfam profile PF05832: Eukaryotic protein of unknown function Length = 186 Score = 53.6 bits (123), Expect = 1e-07 Identities = 21/54 (38%), Positives = 35/54 (64%) Frame = +2 Query: 368 KQFVHPYIVFFHLVFRCSAIIVYILCGWFSDSFIASFVLVILLLSADFWTVKNI 529 + + +P FH++F+ +A+ YIL F +SF+ FV+ +LL + DFW VKN+ Sbjct: 12 ENYANPRTCLFHVLFKGAALAFYILSALFFNSFVIIFVVTVLLAALDFWVVKNV 65 Score = 53.6 bits (123), Expect = 1e-07 Identities = 23/44 (52%), Positives = 32/44 (72%), Gaps = 4/44 (9%) Frame = +3 Query: 531 SGRLLVGLRWWNYVDDNGKSPWVFEARQ----SRVNRNESRLFW 650 SGR+LVGLRWWN ++D G+S W FE+ +R+N+ +S LFW Sbjct: 66 SGRILVGLRWWNEINDLGESVWKFESLDQESLARMNKKDSWLFW 109 >At1g16180.1 68414.m01938 TMS membrane family protein / tumour differentially expressed (TDE) family protein contains Pfam domain, PF03348: TMS membrane protein/tumour differentially expressed protein (TDE) Length = 412 Score = 32.7 bits (71), Expect = 0.25 Identities = 19/58 (32%), Positives = 30/58 (51%), Gaps = 1/58 (1%) Frame = +2 Query: 281 LMNHPAMNSATVPLLDDDTIAFGEEDNANKQFVHPYIVFFHLVFRCSAII-VYILCGW 451 L++ P A PLL D A +E+ NK+ V FFH++F +++ +L GW Sbjct: 308 LLSPPDSPRAEKPLLPIDGKAEEKEEKENKKPVSYSYAFFHIIFSLASMYSAMLLTGW 365 >At4g06676.1 68417.m01071 expressed protein ; expression supported by MPSS Length = 258 Score = 31.5 bits (68), Expect = 0.57 Identities = 17/51 (33%), Positives = 24/51 (47%) Frame = -3 Query: 543 VTDH*IFLTVQKSADSSRMTNTKLAMNESENHPHRMYTIIAEHRNTRWKNT 391 V H +FL + S DS +N + A+ S+ H H A + T KNT Sbjct: 204 VDSHTVFLVILSSTDSC-FSNFRAALRSSDTHAHGRRHAAANSKATDMKNT 253 >At1g07010.1 68414.m00746 calcineurin-like phosphoesterase family protein contains Pfam profile: PF00149 calcineurin-like phosphoesterase Length = 389 Score = 29.5 bits (63), Expect = 2.3 Identities = 9/20 (45%), Positives = 15/20 (75%) Frame = +3 Query: 594 WVFEARQSRVNRNESRLFWD 653 W+FE+RQ + +R S+ +WD Sbjct: 179 WIFESRQWKEDRRSSQTYWD 198 >At1g27040.2 68414.m03296 nitrate transporter, putative contains Pfam profile: PF00854 POT family; similar to nitrate transporter (NTL1) GI:3377517 [Arabidopsis thaliana] Length = 563 Score = 28.3 bits (60), Expect = 5.3 Identities = 10/34 (29%), Positives = 23/34 (67%) Frame = +2 Query: 410 FRCSAIIVYILCGWFSDSFIASFVLVILLLSADF 511 F +A ++ +L G+ +D+F ++FV+ ++ S +F Sbjct: 73 FMATAFLLALLGGFLADAFFSTFVIFLISASIEF 106 >At1g27040.1 68414.m03297 nitrate transporter, putative contains Pfam profile: PF00854 POT family; similar to nitrate transporter (NTL1) GI:3377517 [Arabidopsis thaliana] Length = 567 Score = 28.3 bits (60), Expect = 5.3 Identities = 10/34 (29%), Positives = 23/34 (67%) Frame = +2 Query: 410 FRCSAIIVYILCGWFSDSFIASFVLVILLLSADF 511 F +A ++ +L G+ +D+F ++FV+ ++ S +F Sbjct: 77 FMATAFLLALLGGFLADAFFSTFVIFLISASIEF 110 >At2g34720.1 68415.m04264 CCAAT-binding transcription factor (CBF-B/NF-YA) family protein contains Pfam profile: PF02045 CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B Length = 198 Score = 27.9 bits (59), Expect = 7.0 Identities = 14/32 (43%), Positives = 17/32 (53%) Frame = +1 Query: 403 PGVPMLCDNCVHPVWVVLRLIHCKLRISHSTA 498 PGVP+ CD PV+V + H LR S A Sbjct: 87 PGVPLQCDAVEEPVFVNAKQYHGILRRRQSRA 118 >At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein / VPS11 family protein similar to Vacuolar protein sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo sapiens]; similar to Vacuolar biogenesis protein END1 (PEP5 protein) (Vacuolar protein sorting 11) (Swiss-Prot:P12868) [Saccharomyces cerevisiae] Length = 932 Score = 27.9 bits (59), Expect = 7.0 Identities = 13/44 (29%), Positives = 25/44 (56%) Frame = +3 Query: 132 IVCDLSNFQDYLLFLYNSICLTAVEIIDQYLSTYSDTHLKLICE 263 I+C++++F+D LL+LY + E+I Y+ + L C+ Sbjct: 684 ILCEMNSFKDGLLYLYEKMKFYK-EVIACYMQNHDHEGLIACCK 726 >At2g20210.1 68415.m02363 leucine-rich repeat family protein contains leucine rich repeat (LRR) domains, Pfam:PF00560 Length = 604 Score = 27.5 bits (58), Expect = 9.2 Identities = 11/20 (55%), Positives = 14/20 (70%) Frame = +1 Query: 454 LRLIHCKLRISHSTAVCRLL 513 L LIHCKL +S +A+C L Sbjct: 233 LELIHCKLSLSSISAICTSL 252 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,051,310 Number of Sequences: 28952 Number of extensions: 310972 Number of successful extensions: 658 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 654 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 658 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1526202912 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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