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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0584
         (707 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g09330.1 68414.m01044 expressed protein contains 3 transmembr...    54   1e-07
At1g16180.1 68414.m01938 TMS membrane family protein / tumour di...    33   0.25 
At4g06676.1 68417.m01071 expressed protein ; expression supporte...    31   0.57 
At1g07010.1 68414.m00746 calcineurin-like phosphoesterase family...    29   2.3  
At1g27040.2 68414.m03296 nitrate transporter, putative contains ...    28   5.3  
At1g27040.1 68414.m03297 nitrate transporter, putative contains ...    28   5.3  
At2g34720.1 68415.m04264 CCAAT-binding transcription factor (CBF...    28   7.0  
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot...    28   7.0  
At2g20210.1 68415.m02363 leucine-rich repeat family protein cont...    27   9.2  

>At1g09330.1 68414.m01044 expressed protein contains 3 transmembrane
           domains; contains Pfam profile PF05832: Eukaryotic
           protein of unknown function
          Length = 186

 Score = 53.6 bits (123), Expect = 1e-07
 Identities = 21/54 (38%), Positives = 35/54 (64%)
 Frame = +2

Query: 368 KQFVHPYIVFFHLVFRCSAIIVYILCGWFSDSFIASFVLVILLLSADFWTVKNI 529
           + + +P    FH++F+ +A+  YIL   F +SF+  FV+ +LL + DFW VKN+
Sbjct: 12  ENYANPRTCLFHVLFKGAALAFYILSALFFNSFVIIFVVTVLLAALDFWVVKNV 65



 Score = 53.6 bits (123), Expect = 1e-07
 Identities = 23/44 (52%), Positives = 32/44 (72%), Gaps = 4/44 (9%)
 Frame = +3

Query: 531 SGRLLVGLRWWNYVDDNGKSPWVFEARQ----SRVNRNESRLFW 650
           SGR+LVGLRWWN ++D G+S W FE+      +R+N+ +S LFW
Sbjct: 66  SGRILVGLRWWNEINDLGESVWKFESLDQESLARMNKKDSWLFW 109


>At1g16180.1 68414.m01938 TMS membrane family protein / tumour
           differentially expressed (TDE) family protein contains
           Pfam domain, PF03348: TMS membrane protein/tumour
           differentially expressed protein (TDE)
          Length = 412

 Score = 32.7 bits (71), Expect = 0.25
 Identities = 19/58 (32%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
 Frame = +2

Query: 281 LMNHPAMNSATVPLLDDDTIAFGEEDNANKQFVHPYIVFFHLVFRCSAII-VYILCGW 451
           L++ P    A  PLL  D  A  +E+  NK+ V     FFH++F  +++    +L GW
Sbjct: 308 LLSPPDSPRAEKPLLPIDGKAEEKEEKENKKPVSYSYAFFHIIFSLASMYSAMLLTGW 365


>At4g06676.1 68417.m01071 expressed protein ; expression supported
           by MPSS
          Length = 258

 Score = 31.5 bits (68), Expect = 0.57
 Identities = 17/51 (33%), Positives = 24/51 (47%)
 Frame = -3

Query: 543 VTDH*IFLTVQKSADSSRMTNTKLAMNESENHPHRMYTIIAEHRNTRWKNT 391
           V  H +FL +  S DS   +N + A+  S+ H H      A  + T  KNT
Sbjct: 204 VDSHTVFLVILSSTDSC-FSNFRAALRSSDTHAHGRRHAAANSKATDMKNT 253


>At1g07010.1 68414.m00746 calcineurin-like phosphoesterase family
           protein contains Pfam profile: PF00149 calcineurin-like
           phosphoesterase
          Length = 389

 Score = 29.5 bits (63), Expect = 2.3
 Identities = 9/20 (45%), Positives = 15/20 (75%)
 Frame = +3

Query: 594 WVFEARQSRVNRNESRLFWD 653
           W+FE+RQ + +R  S+ +WD
Sbjct: 179 WIFESRQWKEDRRSSQTYWD 198


>At1g27040.2 68414.m03296 nitrate transporter, putative contains
           Pfam profile: PF00854 POT family; similar to nitrate
           transporter (NTL1) GI:3377517 [Arabidopsis thaliana]
          Length = 563

 Score = 28.3 bits (60), Expect = 5.3
 Identities = 10/34 (29%), Positives = 23/34 (67%)
 Frame = +2

Query: 410 FRCSAIIVYILCGWFSDSFIASFVLVILLLSADF 511
           F  +A ++ +L G+ +D+F ++FV+ ++  S +F
Sbjct: 73  FMATAFLLALLGGFLADAFFSTFVIFLISASIEF 106


>At1g27040.1 68414.m03297 nitrate transporter, putative contains
           Pfam profile: PF00854 POT family; similar to nitrate
           transporter (NTL1) GI:3377517 [Arabidopsis thaliana]
          Length = 567

 Score = 28.3 bits (60), Expect = 5.3
 Identities = 10/34 (29%), Positives = 23/34 (67%)
 Frame = +2

Query: 410 FRCSAIIVYILCGWFSDSFIASFVLVILLLSADF 511
           F  +A ++ +L G+ +D+F ++FV+ ++  S +F
Sbjct: 77  FMATAFLLALLGGFLADAFFSTFVIFLISASIEF 110


>At2g34720.1 68415.m04264 CCAAT-binding transcription factor
           (CBF-B/NF-YA) family protein contains Pfam profile:
           PF02045 CCAAT-binding transcription factor (CBF-B/NF-YA)
           subunit B
          Length = 198

 Score = 27.9 bits (59), Expect = 7.0
 Identities = 14/32 (43%), Positives = 17/32 (53%)
 Frame = +1

Query: 403 PGVPMLCDNCVHPVWVVLRLIHCKLRISHSTA 498
           PGVP+ CD    PV+V  +  H  LR   S A
Sbjct: 87  PGVPLQCDAVEEPVFVNAKQYHGILRRRQSRA 118


>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
           / VPS11 family protein similar to Vacuolar protein
           sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
           sapiens]; similar to Vacuolar biogenesis protein END1
           (PEP5 protein) (Vacuolar protein sorting 11)
           (Swiss-Prot:P12868) [Saccharomyces cerevisiae]
          Length = 932

 Score = 27.9 bits (59), Expect = 7.0
 Identities = 13/44 (29%), Positives = 25/44 (56%)
 Frame = +3

Query: 132 IVCDLSNFQDYLLFLYNSICLTAVEIIDQYLSTYSDTHLKLICE 263
           I+C++++F+D LL+LY  +     E+I  Y+  +    L   C+
Sbjct: 684 ILCEMNSFKDGLLYLYEKMKFYK-EVIACYMQNHDHEGLIACCK 726


>At2g20210.1 68415.m02363 leucine-rich repeat family protein
           contains leucine rich repeat (LRR) domains, Pfam:PF00560
          Length = 604

 Score = 27.5 bits (58), Expect = 9.2
 Identities = 11/20 (55%), Positives = 14/20 (70%)
 Frame = +1

Query: 454 LRLIHCKLRISHSTAVCRLL 513
           L LIHCKL +S  +A+C  L
Sbjct: 233 LELIHCKLSLSSISAICTSL 252


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,051,310
Number of Sequences: 28952
Number of extensions: 310972
Number of successful extensions: 658
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 654
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 658
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1526202912
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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