Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0575
         (627 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    92   5e-20
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha...    87   3e-18
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    65   9e-12
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    55   1e-08
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    47   3e-06
SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    29   0.42 
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch...    28   0.96 
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce...    28   1.3  
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    27   2.9  
SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac...    27   2.9  
SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosa...    26   3.9  
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi...    26   5.1  
SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizo...    26   5.1  
SPCC584.16c |||sequence orphan|Schizosaccharomyces pombe|chr 3||...    25   6.8  
SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c...    25   9.0  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 92.3 bits (219), Expect = 5e-20
 Identities = 51/134 (38%), Positives = 77/134 (57%), Gaps = 6/134 (4%)
 Frame = +2

Query: 206 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 373
           + +Q+AEF+EAF L D D+DG I  N+L     SLG+  +  EL +M+ E     +G I+
Sbjct: 7   TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66

Query: 374 FTQLLTLFANRMSGGSDEDDVVINAFKTFDEXGK--IDSERLRHALMTWGDKFSADEVDE 547
           FT+ LT+ A +M   +D ++ V  AFK FD+ G   I  E L H L + G++ S +EV +
Sbjct: 67  FTEFLTMMARKMK-DTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVAD 125

Query: 548 AYDQMDIDDKGYID 589
              + D D  G I+
Sbjct: 126 MIREADTDGDGVIN 139



 Score = 28.3 bits (60), Expect = 0.96
 Identities = 19/54 (35%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
 Frame = +2

Query: 461 DEXGKID-SERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTA 619
           D  G ID +E L        D  + +EV EA+   D D  GYI   +L  +LT+
Sbjct: 60  DGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTS 113


>SPAC926.03 |rlc1||myosin II regulatory light chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 86.6 bits (205), Expect = 3e-18
 Identities = 47/130 (36%), Positives = 70/130 (53%), Gaps = 2/130 (1%)
 Frame = +2

Query: 161 SRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELD 340
           ++ AKR  S  F+  +  Q+ E KEAF L+D D DG IG+ D++    SL + ASE  ++
Sbjct: 28  AQAAKRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSIN 87

Query: 341 EMVGEASGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFD--EXGKIDSERLRHALMTW 514
            M    + PIN    LT   + +   S  +D ++ AF TFD  + GKI    +R AL + 
Sbjct: 88  HMFESINPPINLAAFLTAMGSMLCRISPRND-LLEAFSTFDDTQSGKIPISTMRDALSSM 146

Query: 515 GDKFSADEVD 544
           GD+    EV+
Sbjct: 147 GDRMDPQEVE 156


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 64.9 bits (151), Expect = 9e-12
 Identities = 40/140 (28%), Positives = 71/140 (50%), Gaps = 2/140 (1%)
 Frame = +2

Query: 206 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQL 385
           S++Q  E KEAF L D DKDG+I  + + +   SLG   ++ EL ++  E    I+  + 
Sbjct: 4   SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63

Query: 386 LTLFANRMSGGSDEDDVVINAFKTFDE--XGKIDSERLRHALMTWGDKFSADEVDEAYDQ 559
           ++  +N++     E++  I AF+ FD+   G I++ +    + T G+K S +EV     +
Sbjct: 64  MSFVSNKLRETESEEE-YIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQE 122

Query: 560 MDIDDKGYIDTTKLIAMLTA 619
            D  + G  D    +  + A
Sbjct: 123 ADPTNSGSFDYYDFVQRIMA 142


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 54.8 bits (126), Expect = 1e-08
 Identities = 37/146 (25%), Positives = 69/146 (47%), Gaps = 6/146 (4%)
 Frame = +2

Query: 206 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 373
           +++Q  +  EAF+L D DKD  I  ++LRA   +LG  A + E+ +++ +      G + 
Sbjct: 32  TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91

Query: 374 FTQLLTLFANRMSGGSDEDDVVINAFKTF--DEXGKIDSERLRHALMTWGDKFSADEVDE 547
               + +   ++    D  + +  AF+ F  DE GKI    LR       +     E++ 
Sbjct: 92  MEDFVRVMTEKIV-ERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEA 150

Query: 548 AYDQMDIDDKGYIDTTKLIAMLTASA 625
             ++ D+D  G I+  + IA++   A
Sbjct: 151 MIEEFDLDQDGEINEQEFIAIMMDEA 176


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 46.8 bits (106), Expect = 3e-06
 Identities = 34/136 (25%), Positives = 63/136 (46%), Gaps = 4/136 (2%)
 Frame = +2

Query: 227 FKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANR 406
           +K+AF L D    G I K  +     + G+  +  E+ E+       ++  Q L +  NR
Sbjct: 8   YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTLPAEVDMEQFLQVL-NR 66

Query: 407 MSGGS--DEDDVVINAFKTFDEX--GKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDD 574
            +G     + +  +  F+ FD+   G I    LR+ L + G+K S +E+DE    + + D
Sbjct: 67  PNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKD 126

Query: 575 KGYIDTTKLIAMLTAS 622
            G ++    + M+ A+
Sbjct: 127 -GMVNYHDFVQMILAN 141



 Score = 40.7 bits (91), Expect = 2e-04
 Identities = 18/42 (42%), Positives = 26/42 (61%)
 Frame = +2

Query: 224 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 349
           EF + FQ+ D D  G+IG  +LR    SLG   S +E+DE++
Sbjct: 78  EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119


>SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 452

 Score = 29.5 bits (63), Expect = 0.42
 Identities = 17/47 (36%), Positives = 26/47 (55%)
 Frame = -1

Query: 357 ASPTISSSSFSDASLPRESKVARKSFLPMMPSLSWSISWNASLNSAT 217
           AS    SSS S +++     V+  S  P   + + SISWN+S ++AT
Sbjct: 126 ASNYTQSSSNSTSTMNSTGSVSGGSVYPTNSTTNSSISWNSSTSAAT 172


>SPAC1687.14c |||EF hand family protein, unknown
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 76

 Score = 28.3 bits (60), Expect = 0.96
 Identities = 10/19 (52%), Positives = 14/19 (73%)
 Frame = +2

Query: 533 DEVDEAYDQMDIDDKGYID 589
           +E +EA+D  D+  KGYID
Sbjct: 12  EEAEEAFDLFDVTHKGYID 30



 Score = 27.1 bits (57), Expect = 2.2
 Identities = 15/42 (35%), Positives = 22/42 (52%)
 Frame = +2

Query: 224 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 349
           E +EAF L D    G I   DLR +   LG   ++++L  M+
Sbjct: 13  EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLML 54


>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1036

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 17/46 (36%), Positives = 26/46 (56%)
 Frame = -1

Query: 354 SPTISSSSFSDASLPRESKVARKSFLPMMPSLSWSISWNASLNSAT 217
           S +++SSS + +SL   S  +  S  P   + S S+S  A+ NSAT
Sbjct: 146 SSSLASSSTTSSSLASSSTNSTTSATPTSSATSSSLSSTAASNSAT 191


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 26.6 bits (56), Expect = 2.9
 Identities = 22/83 (26%), Positives = 35/83 (42%)
 Frame = -1

Query: 438 TTSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFSDASLPRESKVARKSFLPMMPSL 259
           +TSSSS      L++   S        +S  ISSSS S +S    S     S        
Sbjct: 242 STSSSSSSTSSSLSSSSSSSTASSSSSSSSIISSSSSSSSSPTSTSSTISSSSSSSSSPT 301

Query: 258 SWSISWNASLNSATCFCENIEKT 190
           S S + ++S +S++ F   +  +
Sbjct: 302 STSSTISSSSSSSSSFSSTLSSS 324


>SPCC736.14 |dis1||microtubule-associated protein Dis1
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 882

 Score = 26.6 bits (56), Expect = 2.9
 Identities = 24/56 (42%), Positives = 31/56 (55%), Gaps = 2/56 (3%)
 Frame = -1

Query: 423 SEPPDMRLANRVK-S*VKL-MGPEASPTISSSSFSDASLPRESKVARKSFLPMMPS 262
           S P   R+A+ +K S VKL + P+A   + SS+ S ASL  ES   R S  P  PS
Sbjct: 542 SRPRLPRVASPLKTSPVKLAVTPQAPSPLPSSNPSQASLTEESLSTRSS--PTKPS 595


>SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein
           Msa1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 533

 Score = 26.2 bits (55), Expect = 3.9
 Identities = 23/63 (36%), Positives = 30/63 (47%)
 Frame = -1

Query: 447 ALITTSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFSDASLPRESKVARKSFLPMM 268
           A I  S SS+PP      R  S V+     A   ++S+ FS    P ES  +  S LP +
Sbjct: 272 APIPFSVSSDPPSTM--GRSNSAVQSPSYFAHSLVNSTEFST---PNESLSSLPSILPSI 326

Query: 267 PSL 259
           PSL
Sbjct: 327 PSL 329


>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 374

 Score = 25.8 bits (54), Expect = 5.1
 Identities = 21/73 (28%), Positives = 35/73 (47%)
 Frame = -1

Query: 435 TSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFSDASLPRESKVARKSFLPMMPSLS 256
           +SSSS       ++   S        +S + SSSS S +S    S  +  S +P+  S S
Sbjct: 156 SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSVPITSSTS 215

Query: 255 WSISWNASLNSAT 217
            S S ++S +S++
Sbjct: 216 SSHSSSSSSSSSS 228


>SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase
           Ubp16|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 457

 Score = 25.8 bits (54), Expect = 5.1
 Identities = 11/33 (33%), Positives = 22/33 (66%), Gaps = 1/33 (3%)
 Frame = -1

Query: 357 ASPTISSSSFSDASLPRESKVARKSF-LPMMPS 262
           +SP++   +++  + P++S V+RKSF  P  P+
Sbjct: 35  SSPSVPEGTYTVLNNPKQSTVSRKSFSAPTSPT 67


>SPCC584.16c |||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 231

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 13/28 (46%), Positives = 16/28 (57%)
 Frame = -1

Query: 342 SSSSFSDASLPRESKVARKSFLPMMPSL 259
           SSSS S +S P   + +RK   P MP L
Sbjct: 3   SSSSSSGSSSPASKRSSRKRQTPPMPEL 30


>SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1274

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 12/38 (31%), Positives = 21/38 (55%)
 Frame = +2

Query: 215 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASE 328
           + A+FK+ F     DKD +  + +L A  + L +LA +
Sbjct: 212 EYAKFKDKFPEAPLDKDNLQTQKELEARIECLKQLAEK 249


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,688,591
Number of Sequences: 5004
Number of extensions: 26921
Number of successful extensions: 163
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 277683324
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)