BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0574 (614 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p... 31 0.17 SPBC21C3.10c |||5-amino-6-|Schizosaccharomyces pombe|chr 2|||Manual 27 2.2 SPAC9G1.09 |sid1||PAK-related kinase Sid1|Schizosaccharomyces po... 25 8.7 SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1 |Schizosaccharom... 25 8.7 SPCC825.03c |psy1|sso1|SNARE Psy1|Schizosaccharomyces pombe|chr ... 25 8.7 >SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1323 Score = 30.7 bits (66), Expect = 0.17 Identities = 20/72 (27%), Positives = 38/72 (52%) Frame = -3 Query: 255 LDRFISQQKHISSSYVRACINKVTLCNVTKKNTPDLNALKLSKI*NPYYRRKRALLHLQR 76 + +++ ++ +++ YV +KVT+CN + + N +L+K+ + R L HL R Sbjct: 984 MSKYLPVKRALAAEYVVDTGDKVTVCNKNAEVSAKHNYYRLAKV---WLMLGRLLGHLSR 1040 Query: 75 GRAKDKHLK*EE 40 KD H+ EE Sbjct: 1041 TENKD-HINDEE 1051 >SPBC21C3.10c |||5-amino-6-|Schizosaccharomyces pombe|chr 2|||Manual Length = 268 Score = 27.1 bits (57), Expect = 2.2 Identities = 11/27 (40%), Positives = 17/27 (62%) Frame = +1 Query: 223 DMLLLRDEAVEDASPSGTAVLPNPGNP 303 D +++ E+ +PS TA LP+P NP Sbjct: 60 DGIMVGSRTAENDNPSLTAKLPDPANP 86 >SPAC9G1.09 |sid1||PAK-related kinase Sid1|Schizosaccharomyces pombe|chr 1|||Manual Length = 471 Score = 25.0 bits (52), Expect = 8.7 Identities = 12/22 (54%), Positives = 14/22 (63%) Frame = -2 Query: 376 NATHKKRQRRSEGSFL*F*RWL 311 N H K+Q+RS S L F RWL Sbjct: 439 NRLHHKKQKRSPISQLLFSRWL 460 >SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 787 Score = 25.0 bits (52), Expect = 8.7 Identities = 13/46 (28%), Positives = 19/46 (41%) Frame = -3 Query: 267 WRGVLDRFISQQKHISSSYVRACINKVTLCNVTKKNTPDLNALKLS 130 WR V IS +K + Y + +T C+ K D L +S Sbjct: 306 WREVTKMLISGKKPSYTKYKKVTAEYITFCDKKFKEDEDAKRLYMS 351 >SPCC825.03c |psy1|sso1|SNARE Psy1|Schizosaccharomyces pombe|chr 3|||Manual Length = 284 Score = 25.0 bits (52), Expect = 8.7 Identities = 7/22 (31%), Positives = 13/22 (59%) Frame = +3 Query: 351 LCLFLCVAFVCAVLVETCLVLV 416 +C +CV +C ++ C VL+ Sbjct: 256 ICFGICVVIICVIVAVLCGVLI 277 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,399,205 Number of Sequences: 5004 Number of extensions: 46295 Number of successful extensions: 111 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 111 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 111 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 269634532 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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