Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0554
(783 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC30D11.06c |||DUF300 family protein|Schizosaccharomyces pombe... 88 1e-18
SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr 3|||M... 29 0.57
SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3 B... 27 2.3
SPCC330.11 |btb1||BTB/POZ domain protein Btb1|Schizosaccharomyce... 27 2.3
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 27 4.0
SPBC409.11 |meu18||sequence orphan|Schizosaccharomyces pombe|chr... 26 7.0
SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual 26 7.0
SPAC27F1.08 |pdt1||Nramp family manganese ion transporter|Schizo... 26 7.0
SPCC18B5.11c |cds1||replication checkpoint kinase Cds1|Schizosac... 25 9.3
>SPAC30D11.06c |||DUF300 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 426
Score = 88.2 bits (209), Expect = 1e-18
Identities = 64/227 (28%), Positives = 116/227 (51%), Gaps = 6/227 (2%)
Frame = +3
Query: 21 KHIIRILWMVPIYALNALIGLEFPEQSIYLDSLRECYEAYVIYNFMKYLLNYLNDGHDLE 200
+ ++RIL M+ IY+ + + + + + RE YEA+ +Y F L++YL G +
Sbjct: 38 RSVVRILMMIVIYSSVSFLSVYNEKIGSIFEPFREIYEAFALYCFFCLLIDYL--GGERA 95
Query: 201 AVLETKPQVYH--IFPLCCLAP-WEMGREFVH-NCKHGILQYTLVRPITTVISMICELND 368
AV+ + ++PL L ++ + + K GILQYT ++P + ++ ++
Sbjct: 96 AVISLHGHLPRPRLWPLNYLQDDIDLSDPYTFLSIKRGILQYTWLKPFLVIAVLLTKVTG 155
Query: 369 VYGESDFKPNVAFP--YIIAINNLSQFVAMYCLVLFYKANRTELKPMKPIGKFLCIKAVV 542
VY D +P A +I + N+S +++Y L F+ EL P +P KFL +KA++
Sbjct: 156 VYDRED-QPVYASADLWIGLVYNISITLSLYSLTTFWVCLHEELAPFRPFPKFLSVKAII 214
Query: 543 FFSFFQGVIITILVYCGVLKTIFDISDTDTVKIISSKLQDFLICIEM 683
F S++Q +++I + G+L T I S Q+ L+C+EM
Sbjct: 215 FASYWQQTVLSITNWLGLL--------NGTGWIYSLLNQNVLMCLEM 253
>SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 535
Score = 29.5 bits (63), Expect = 0.57
Identities = 16/66 (24%), Positives = 35/66 (53%)
Frame = +3
Query: 492 LKPMKPIGKFLCIKAVVFFSFFQGVIITILVYCGVLKTIFDISDTDTVKIISSKLQDFLI 671
L+P+ + ++ +FF F+ ++++IL++C + +F + + + S LQ L
Sbjct: 242 LEPLVHLHEYSRRSNTLFF-FYLPIVMSILIFCLPTQALFIVLSSVFLAWHSPPLQAVLF 300
Query: 672 CIEMVS 689
CI +S
Sbjct: 301 CIRRIS 306
>SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3
Brl1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 692
Score = 27.5 bits (58), Expect = 2.3
Identities = 15/41 (36%), Positives = 23/41 (56%)
Frame = -2
Query: 563 SLEKREKNNSFYT*EFPNRLHWFELSSVCLVKEYQTVHSYE 441
+LEK KNNS E P+ F+ S L+K+Y+ + + E
Sbjct: 435 ALEKLIKNNSCLEAELPSMYAAFDQSQSRLLKKYEELETKE 475
>SPCC330.11 |btb1||BTB/POZ domain protein Btb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1347
Score = 27.5 bits (58), Expect = 2.3
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +2
Query: 398 CSISIYHSHKQSITIRSYVLFGTLLQGKQN 487
C S++HSHK + RS L LLQ K++
Sbjct: 625 CGTSMFHSHKFILCARSSFLRKLLLQKKKS 654
>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1133
Score = 26.6 bits (56), Expect = 4.0
Identities = 14/52 (26%), Positives = 24/52 (46%)
Frame = -2
Query: 314 VLKYSMLAVMYKFPPHFPWC*ATQWKYMIYLRFCLQHSF*VMAIIQVV*EIL 159
V K L + KF + PWC T W+ + L + + + ++Q + E L
Sbjct: 689 VNKLDDLYSLIKFMRYEPWCNYTYWQTFVSLPYQSKDVLKALNVVQSILEFL 740
>SPBC409.11 |meu18||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 553
Score = 25.8 bits (54), Expect = 7.0
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = -3
Query: 715 NYSDALSPPETISIHMRKSCSFED 644
NY DA + PE ISI S + ED
Sbjct: 381 NYDDAFAAPEAISIEKYVSINEED 404
>SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 978
Score = 25.8 bits (54), Expect = 7.0
Identities = 8/20 (40%), Positives = 14/20 (70%)
Frame = +3
Query: 135 AYVIYNFMKYLLNYLNDGHD 194
++ +YN YL+ YL+D +D
Sbjct: 325 SFQLYNLFPYLIRYLSDDYD 344
>SPAC27F1.08 |pdt1||Nramp family manganese ion
transporter|Schizosaccharomyces pombe|chr 1|||Manual
Length = 521
Score = 25.8 bits (54), Expect = 7.0
Identities = 11/35 (31%), Positives = 23/35 (65%), Gaps = 1/35 (2%)
Frame = -3
Query: 493 SSVLFAL*KSTKQYIATNCDRLF-MAMIYGNATLG 392
+S LF++ K+Y++ +C RLF +A+++ + G
Sbjct: 352 TSDLFSIYDLLKEYVSISCGRLFAVALLFSGMSAG 386
>SPCC18B5.11c |cds1||replication checkpoint kinase
Cds1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 25.4 bits (53), Expect = 9.3
Identities = 11/36 (30%), Positives = 17/36 (47%)
Frame = +2
Query: 500 NEAYWEILMYKSCCFFLFFPRSDNHHIGLLRCLKND 607
+ +W +KSC L PR N H + + +ND
Sbjct: 56 HNGFWRFGRHKSCEVVLNGPRVSNFHFEIYQGHRND 91
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,265,064
Number of Sequences: 5004
Number of extensions: 69508
Number of successful extensions: 172
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 379359666
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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